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http://dx.doi.org/10.15204/jkobgy.2010.23.3.038

Anti-cancer Effects of Samultang-Gami on HeLa, HepG2, AGS Cells  

Jung, Jae-Joong (Dept. of OB & GY, Graduate school of Oriental Medicine, Dong-Guk Univ.)
Goo, Sun-Young (Dept. of Life Science, Dong-Guk Univ.)
Goo, Sun-Young (Dept. of Life Science, Dong-Guk Univ.)
Sung, Jung-Suk (Dept. of Life Science, Dong-Guk Univ.)
Kim, Dong-Il (Dept. of OB & GY, Graduate school of Oriental Medicine, Dong-Guk Univ.)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.23, no.3, 2010 , pp. 38-55 More about this Journal
Abstract
Purpose: This study was designed to find out the anti-cancer effects of Samultang-Gami which was composed of Rehmanniae Radix(RR), Angelicae Gigantis Radix(AGR), Cnidii Rhizoma(CR), Paeoniae Radix(PR), Cortex Moutan Radicis(CMR), Hedyotis Diffusa(HD) and Caesalpinia Sappan on HeLa, HepG2 and AGS cells. Methods: Various cancer cell lines including HeLa, HepG2 and AGS cells, were used. In vitro anti-cancer effects were measured by MTT assay using cancer cell lines treated with various concentrations of 70% ethanol extract of Samultang-Gami. Expression of cell cycle arrest mediators including Bax, Bcl-2, p53 and DARP-1 proteins were measured by Western blot analysis. Results: 1. Samultang-Gami decreased the viability of HeLa and HepG cells in a dosedependent manner. 2. AGR, CMR, PR and HD decreased the viability of HeLa, HepG2 and AGS cells. 3. We could observe that the decreased Bax and Bcl-2 expression level and the increased PARP-1 expression level by Samultang-Gami extracts treated in HeLa cells. 4. We could observe that the decreased Bcl-2 expression level and the increased Bax, p53 and PARP-1 expression level by RR extracts treated in HeLa cells. and also could observe that the reduction of the protein level of Bcl-2, p53 and PARP-1 and the increase of the protein level of Bax by PR in HeLa cells. 5. We could observe that the increased p53 expression level, the decreased PARP-1's that and the unchanged Bax and Bcl-2's that by Samultang-Gami extracts treated in HepG2 cells. 6. We could observe that the reduced Bcl-2 expression level by each of RR extracts and PR extracts in HepG2 cells. 7. The treatment of Samultang-Gami in AGS cells didn't have any effect on the expression level of Bax, Bcl-2, p53 and PARP-1. 8. We could observe that the increased p53 and PARP-1 expression level by each of CR, RR and PR extracts in AGS cells. Conclusion: Taken together, we suggest that Samultang-Gami exhibits cytotoxic effects on HeLa, HepG2 and AGS cells, causing apoptosis. The results showed that Samultang-Gami may do so by regulating the expression of specific target molecules that promote efficient apoptotic cell death in a dose-dependent manner.
Keywords
Samultang-Gami; HeLa; HepG2; AGS; Apoptosis; Bax; Bcl-2; p53; PARP-1; anti-cancer;
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Times Cited By KSCI : 9  (Citation Analysis)
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