• Proceedings of the Korean Society for Bioinformatics Conference
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• 2006.02a
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• pp.83-89
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• 2006

Cellular functions are carried out by a concerted action of biochemical pathways whose components have genetic interactions. Abnormalities in the activity of the genes that constitute or modulate these pathways frequently have oncogenic implications. Therefore, identifying the upstream regulatory genes for major biochemical pathways and defining their roles in carcinogenesis can have important consequences in establishing an effective target-oriented antitumor strategy We have analyzed the gene expression profiles of human liver cancer samples using cDNA microarray chips enriched in liver and/or stomach-expressed cDNA elements, and identified groups of genes that can tell tumors from non-tumors or normal liver, or classify tumors according to clinical parameters such as tumor grade, age, and inflammation grade. We also set up a high-throughput cell-based assay system (cell chip) that can monitor the activity of major biochemical pathways through a reporter assay. Then, we applied the cell chip platform for the analysis of the HCC-associated genes discovered from transcriptome profiling, and found a number of cancer marker genes having a potential of modulating the activity of cancer-related biochemical pathways such as E2F, TCF, p53, Stat, Smad, AP-1, c-Myc, HIF and NF-kB. Some of these marker genes were previously blown to modulate these pathways, while most of the others not. Upon a fast-track phenotype analysis, a subset of the genes showed increased colony forming abilities in soft agar and altered cell morphology or adherence characteristics in the presence of purified matrix proteins. We are currently analyzing these selected marker genes in more detail for their effects on various biological Processes and for Possible clinical roles in liver cancer development.

• Journal of the Korean Institute of Intelligent Systems
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• v.22 no.2
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• pp.192-197
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• 2012

With the advent of DNA microarray technologies, researches for disease diagnosis has been actively in progress. In typical experiments using microarray data, problems such as the large number of genes and the relatively small number of samples, the inherent measurement noise and the heterogeneity across different samples are the cause of the performance decrease. To overcome these problems, a new method using functional modules (e.g. signaling pathways) used as markers was proposed. They use the method using an activity of pathway summarizing values of a member gene's expression values. It showed better classification performance than the existing methods based on individual genes. The activity calculation, however, used in the method has some drawbacks such as a correlation between individual genes and each phenotype is ignored and characteristics of individual genes are removed. In this paper, we propose a method based on the ensemble classifier. It makes weak classifiers based on feature vectors using subsets of genes in selected pathways, and then infers the final classification result by combining the results of each weak classifier. In this process, we improved the performance by minimize the search space through a filtering process using gene-gene interaction information and the mRMR filter. We applied the proposed method to a classifying the lung cancer, it showed competitive classification performance compared to existing methods.

• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.298-303
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• 2010

To explore on the defense strategies against hervivory of Juglans regia and J. mandshurica, morphological characteristics of the leaf, leaf domatia structure and the number, herbivores insects and mites on the leaves, collected from the trees growing in Mt. Chiak, Mt. Cheongtae, Mt. Jungwang, Namyangju-si and Wonju-si, were investigated from May to October, 2009. Domatia of J. mandshurica revealed tuft type, these of J. regia revealed pocket+tuft type. Domatia number per leaflet proved the higher figures, 28.3/leaflet for J. mandshurica, and the lower, 19.6/leaflet for J. regia. Leaf surface trichomes of J. regia revealed stellates only on the domatia structures, and that of J. mandshurica does dense stellates and glandular hairs on the leaf-blade and vein. Predatory mites' number per leaflet proved highly significant differences among tree species, and mean of predatory mites was higher values (4.8/leaflet) in J. mandshurica and lower values (3.5/leaflet) in J. regia. Small amount of nectar are found distal veinparts on the leaf margin of J. mandshurica. Dense glandular hairs on the lower leaf surfaces of J. mandshurica estimates useful strategy against herbivory. It may be possible to breed J. regia for better expression of leaf traits such as dense glandular hairs that increase predator populations and efficacy.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.59-59
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• 2018

Panax ginseng Meyer (Korean ginseng) is in the spotlight of Oriental medicine and is proclaimed as the king of medicinal plants owing to its adaptogenic characteristics. Ginseng root rot is a devastating disease caused by the fungus, Ilyonectria mors-panacis that generally attacks younger roots (~2 years), leading to defects in root quality, ginsenoside accumulation and also life cycle of the plant. Hence, there is an indispensable need to develop strategies resulting in tolerance against ginseng root rot. In the present study, we evaluated the effect of silica nanoparticles(N-SiO2) in Panax ginseng during I. mors-panacis infection. Long term analysis (30 dpi) revealed a striking 50% reduction in disease severity index upon 1mM and 2mM treatment of N-SiO2. However, N-SiO2 did not have any direct antifungal activity against I. mors-panacis. Membrane bound sugar efflux transporter, SWEET (Sugars Will Eventually be Exported Transporters) was identified in ginseng and as expected, its expression was suppressed upon N-SiO2 treatment in the root rot pathosystem. Furthermore, the total and reducing sugars in the apoplastic fluid clearly revealed that N-SiO2 regulates sugar efflux into apoplast. In a nut shell, N-SiO2 administration induces transcriptional reprogramming in ginseng roots, leading to regulated sugar efflux into apoplast resulting in enhanced tolerance against I. mors-panacis.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.413-419
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• 2016

Ryanodine receptors (RyRs) regulate the contractions of insect muscles by altering intracellular $Ca^{2+}$ concentration and are the targets of chlorantraniliprole. Recently, a chlorantraniliprole-resistant strain was reported in the diamondback moth Plutella xylostella by obtaining point mutations on the RyRs. In the present study, we established two resistant strains from Drosophila melanogaster, which were treated with low or high concentrations of chlorantraniliprole, and their resistance levels were determined on the basis of contact and ingestion toxicities. Compared with the control strain, the two resistant strains did not show any significant differences in contact toxicity. However, they showed significantly increased resistance ratios in ingestion toxicity than that by the control strain. The low and high concentration resistant strains exhibited 2.1- and 8.1-fold increased resistance ratios, respectively, compared with that by the control strain. Moreover, we found that the resistant strains had altered expression levels of RyRs and more enhanced Acetylcholinesterase and Glutathione-S-transferase activities than that by the non-selected strain. These results suggested that the resistance development of chlorantraniliprole in the two strains might be mediated by the activation of detoxification pathways in D. melanogaster.

• Korean journal of communication and information
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• v.47
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• pp.120-147
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• 2009

The purpose of this paper is to promote bilateral friendly relations, the elements that hinder Hate Korea wave's(Kenkanryu) status and an analyzation of the characteristics of its causes and solutions for it. The Hate Korea wave is to be discussed in the fountain Hate Korea wave 2ch(2channel, ni channeru) acquaintances of information and representation to analyze the causes of this Japanese anti-Korean thinking. All of society should be aware of the differences and see what we have in common. I researched discussions of 2ch information and the content of other similar media. The expression was very violent. However, there was both general awareness of a gap between criticism and support. The gap was about what is not unusual on the Internet and not to be greatly concerned about. I also compares anti-Korean's awareness to Korea with general Japanese's, and finds that there are gap between them. For example by analyzing general Japanese's awareness we can find 18% of people who criticize the Hate Korea wave or have positive image of Korea. futhermore the proportion of anti-Korean contents in internet is not very high, so we don't have to worrying about the phenomenon seriously. but because of the following three points, there are sufficient needs to concern about the phenomenon constantly. First, sources of Hate Korea wave are traditional media. Second, the consumers of Hate Korea wave are mainly young generation who will make future relation of two countries. And last, Hate Korea wave include potential power to explode when certain conditions are met.

• IMMUNE NETWORK
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• v.6 no.2
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• pp.59-66
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• 2006

Background: CM1 (Centrocyte/-blast Marker I) defined by a mAb developed against concanavalin-A activated PBMC, is expressed specifically on a subpopulation of centroblasts and centrocytes of human germinal center (GC) B cells. Burkitt lymphoma (BL) is a tumor consisting of tumor cells with the characteristics of GC B cell. Previously we reported that CM1 ligation with anti-CM1 mAb induced apoptosis in Ramos $(IgM^{high})$ and Raji $(IgM^{low})$ cells. Methods & Results: In the present study, we observed that CM1 ligation with anti-CM1 mAb induced Fas ligand and Fas expression in Ramos cells, but not in Raji cells. Furthermore, anti-Fas blocking antibody, ZB4, blocked CM1-mediated apoptosis effectively in Ramos cells, but not in Raji cells. Increased mitochondrial membrane permeabilization, which was measured by $DiOC_6$, was observed only in Raji cells. In contrast to no significant change of Bax known as pro-apoptotic protein, anti-apoptotic protein Bcl-2 was significantly decreased in Raji cells. In addition, we observed that CM1 ligation increased release of mitochondrial cytochrome c and upregulated caspase-9 activity in Raji cells. Conclusion: These results suggest that apoptosis induced by CM1-ligation is mediated by Fas-Fas ligand interaction in Ramos cells, whereas apoptosis is mediated by down-regulation of Bcl-2 and subsequent decrease of mitochondrial membrane potential in Raji cells.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.793-796
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• 2008

Recently early strength concrete has been required for economical assurance and the prevention of frost damage in winter through air reduction in construction of concrete structures. This study presented the optimum condition revealing compressive strength 5MPa which has the possibility of removal of form in 24 hours, and researched the changes of unit weight of cement types of high early strength AE water reducing agents, characteristic of compressive strength expression as cure temperature conditions and slump or airspace. Test results showed at $15^{\circ}C$ with compressive strength of 5MPa that premature removal of form was possible in case of using highly early strength PC water reducing agent with unit weight of cement 360 ; 22hours faster than 10, unit weight 360 ; 20hours faster than 7, unit weight 390 ; 18 hours faster than 4 comparing with OP water reducing agent. And at $5^{\circ}C$ in case of using highly early strength PC water reducing agent with unit weight of cement 330 ; 32hours faster than 10, unit weight 360 ; 30hours faster than 7, unit weight390 ; 27hours faster than 4 comparing with OP water reducing agent. Therefore as the temperature rises $10^{\circ}C$, compressive strength of 5MPa reaching hour shortens 10 hours.

• Journal of Information Technology and Architecture
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• v.10 no.1
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• pp.101-111
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• 2013

Internet traffic volume has been increasing rapidly due to popularization of various smart devices and Internet development. In particular, HTTP-based traffic volume of smart devices is increasing rapidly in addition to desktop traffic volume. The increased mobile traffic can cause serious problems such as network overload, web security, and QoS. In order to solve these problems of the Internet overload and security, it is necessary to accurately detect applications. Traditionally, well-known port based method is utilized in traffic classification. However, this method shows low accuracy since P2P applications exploit a TCP/80 port, which is used for the HTTP protocol; to avoid firewall or IDS. Signature-based method is proposed to solve the lower accuracy problem. This method shows higher analysis rate but it has overhead of signature generation. Also, previous signature-based study only analyzes applications in HTTP protocol-level not application-level. That is, it is difficult to identify application name. Therefore, previous study only performs protocol-level analysis. In this paper, we propose a signature generation method to classify HTTP-based traffics in application-level using the characteristics of typical semi HTTP header. By applying our proposed method to campus network traffic, we validate feasibility of our method.

• Korean Journal of Agricultural Science
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• v.28 no.2
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• pp.78-84
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• 2001

This study was analyzed by PCR technique with specific primer in order to investigate the characteristics of ${\beta}$-lactoglobulin (${\beta}$-LG) gene 5'flanking region in Korean native goat. This work confirmed amplified product of 1,077 bp fragments obtained from the amplification of ${\beta}$-LG promoter from genomic DNA using PCR in Korean native goat. The nucleotide sequence of ${\beta}$-LG gene 5'flanking region in Korean native goat as compared with that of sheep ${\beta}$-LG were different at 46 base of 897 nucleotides, and showed high homology as about 94.9% each breed. Especially we confirmed that the difference of nucleotide sequences between Korean native goat and sheep were consisted of $T{\rightarrow}C$ substitution and $C{\rightarrow}T$ substitution reversely. As a consequences, the sequences of ${\beta}$-LG gene 5'flanking region showed a high homology between Korean native goat and sheep. Furthermore we should be studied that relationships between the control of gene expression and nucleotide sequences of transcription factor in Korean native goat.