• Title/Summary/Keyword: experimentation

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Probiotic Properties and Immunomodulator Evaluation of the Potential Feed Additive Pediococcus acidilactici SRCM102607 (잠재적 사료첨가제로서 Pediococcus acidilactici SRCM102607의 생균제 특성 및 면역활성 효과)

  • Shin, Su-Jin;Ha, Gwangsu;Jeong, Su-Ji;Ryu, Myeong Seon;Kim, Jinwon;Yang, Hee-Jong;Kwak, Mi-Sun;Sung, Moon-Hee;Jeong, Do-Youn
    • Journal of Life Science
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    • v.30 no.10
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    • pp.896-904
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    • 2020
  • The purpose of this study was to investigate the probiotic characteristics and immune activities of selected lactic acid bacterial (LAB) strains as feed additives in livestock. 301 LAB strains isolated from traditional fermented foods were first assessed for their antibacterial activity potential. Of the 301 isolates, five showed antibacterial activity against five livestock pathogens (Esherichia coli KCCM11234, Listeria monocytogens KCTC3710, Salmonella Typhimurium KCTC1926, Staphylococcus aureus KCCM11593, and Shigella flexneri KCTC2517). The probiotic characteristics of the five selected strains were also investigated by antioxidative activity, hemolysis, bile salt hydrolase, acid resistance and bile tolerance. The SRCM102607 strain was found to have superior probiotic properties and was selected for further experimentation. 16S rRNA gene sequencing showed that SRCM102607 is Pediococcus acidilactici, which was labeled as P. acidilactici SRCM102607 (KCCM 12246P). The survival characteristics of P. acidilactici SRCM102607 in artificial gastrointestinal conditions were assessed under exposed acidic (pH 2.0) and bile (0.5% and 1.0%) conditions. P. acidilactici SRCM102607 was also confirmed to have resistance to various antibiotics, including amikacin, gentamicin, vancomycin, and etc. The TNF-α production by P. acidilactici SRCM102607 was 171.86±4.00 ng/ml. These results show that P. acidilactici RCM102607 has excellent potential for use as a probiotic livestock feed additive.

Developing the mathematics model textbook based on storytelling with real-life context - Focusing on the coordinate geometry contents - (실생활 연계형 스토리텔링 수학 교과서 개발 -도형의 방정식 단원을 중심으로-)

  • Kim, Yujung;Kim, Ji Sun;Park, Sang Eui;Park, Kyoo-Hong;Lee, Jaesung
    • Communications of Mathematical Education
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    • v.27 no.3
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    • pp.179-203
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    • 2013
  • The purpose of this study was to discuss the example that developed geometry model textbook based on storytelling using real-life context. To achieve this purpose, we first elaborated the meaning of the textbook based on storytelling with real-life context, and then we discussed the outline of the story and the summary of each lesson. This study defined the storytelling textbook with real-life context as the textbook consisting of activities that explored and organized mathematical concepts by using real-life situations as materials of stories. The geometry textbook we developed employed two real-life materials, a map and a set square: we used a map for the coordinate geometry and a set square for the equation of a line. To attract students' interest, we introduced confrontation between a teacher and two students and a villain. We implemented experimentation with the textbook based on storytelling in order to verify its validity. The participants were 25 students that were enrolled in a high school in Seoul. Among them, 17 participants were surveyed. Students' answers from the survey questionnaire suggested that the geometry textbook we developed based on storytelling helped them learn mathematics and that the instruments such as a map and a set square helped them understand mathematical concepts. However, their opinion implied that the story of the textbook needed to be improved so that the story reflected more realistic contexts that were familiar with students.

Effects of Genistein on Cell Proliferation and Adipogenesis in Mouse 3T3-L1 Preadipocytes (이소플라본 genistein이 전지방세포 성장 및 지방세포형성과정에 미치는 영향)

  • Lim, Seung-Hyun;Kim, Hyo-Rim;Kim, Min-Jeong;Kim, Jong-Sik
    • Journal of Life Science
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    • v.22 no.1
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    • pp.49-54
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    • 2012
  • The effects of genistein on cell proliferation and adipogenesis were examined in mouse 3T3-L1 preadipocyte cells. Genistein decreased viability of 3T3-L1 pre-adipocytes in a dose-dependent manner. Oil Red O staining of these cells also indicated that adipogenesis was inhibited by 50 ${\mu}M$ genistein treatment. We investigated the molecular mechanisms involved in the decrease in cell viability in genistein-treated 3T3-L1 cells by conducting an oligo DNA microarray analysis. We selected the sirtuin-1 gene, one of the upregulated genes, for further experimentation because sirtuin-1 belongs to the sirtuin family, which is associated with anti-obesity and anti-inflammation activities. We found that four phytochemicals (resveratrol, capsaicin, daidzein, and genistein) could increase sirtuin-1 expression. Genistein was the strongest inducer of sirtuin-1 among the tested phytochemicals. The inhibition of adipogenesis by genistein was recovered by surtuin-1 siRNA transfection. Overall, these results may further our understanding of the molecular mechanisms underlying the inhibition of proliferation and adipogenesis by genistein in mouse 3T3-L1 cells.

Fuzzy PD+I Control Method for Two-wheel Balancing Mobile Robot (퍼지 PD+I 제어 방식을 적용한 Two-wheel Balancing Mobile Robot)

  • Eom, Ki-Hwan;Lee, Kyu-Yun;Lee, Hyun-Kwan;Kim, Joo-Woong
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.45 no.1
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    • pp.1-8
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    • 2008
  • A two-wheel balancing vehicle, which helps people moving freely and fast, and is applied from inverted pendulum system, has been widely researched and developed, and some products are came into a market in actuality. Until now, the two-wheel balancing vehicles developed have chosen the general PID control method. In this paper, we propose a new control method to improve a control capacity for a two-wheeled balancing vehicle for human transportation. The proposed method is the fuzzy PD+I control that is one of the improved PID control, and it contains a 2input-1output fuzzy system. This fuzzy system processes signals from proportional and derivative controller, and the fuzzy output signal generates the final output by summing up integral signal. The non-linearity of the fuzzy system makes an optimal output control signal by changing weight of the proportional signal and the derivative signal in process of time. We have simulated the fuzzy PD+I control system and experimented by implementing the two-wheel balancing mobile robot to verify the advantages of the proposed fuzzy PD+I control method in comparison with general PID control. As the results of simulation and experimentation, the proposed fuzzy PD+I control method has better control performance than general PID in this system and improves it.

An ROI Coding Technique of JPEG2000 Image Including Some Arbitrary ROI (임의의 ROI를 포함하는 JPEG2000 이미지의 ROI 코딩 기법)

  • Hong, Seok-Won;Kim, Sang-Bok;Seo, Yeong-Geon
    • Journal of the Korea Society of Computer and Information
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    • v.15 no.11
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    • pp.31-39
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    • 2010
  • In some image processing system or the users who want to see a specific region of image simply, if a part of the image has higher quality than other regions, it would be a nice service. Specifically in mobile environments, preferential service was needed, as the screen size is small. So, JPEG2000 supplies this function. But this doesn't support the process to extract specific regions or service and does the functions to add some techniques. It is called by ROI(Region-of-Interest). In this paper, we use images including human faces, which are processed most preferentially and compressed with high quality. Before an image is served to the users, it is compressed and saved. Here, the face parts are compressed with higher quality than the background which are relatively with lower quality. This technique can offer better service with preferential transferring of the faces, too. Besides, whole regions of the image are compressed with same quality and after searching the faces, they can be preferentially transferred. In this paper, we use a face extraction approach based on neural network and the preferential processing with EBCOT of JPEG2000. For experimentation, we use images having several human faces and evaluate objectively and subjectively, and proved that this approach is a nice one.

Analysis of the CPU/GPU Temperature and Energy Efficiency depending on Executed Applications (응용프로그램 실행에 따른 CPU/GPU의 온도 및 컴퓨터 시스템의 에너지 효율성 분석)

  • Choi, Hong-Jun;Kang, Seung-Gu;Kim, Jong-Myon;Kim, Cheol-Hong
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.5
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    • pp.9-19
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    • 2012
  • As the clock frequency increases, CPU performance improves continuously. However, power and thermal problems in the CPU become more serious as the clock frequency increases. For this reason, utilizing the GPU to reduce the workload of the CPU becomes one of the most popular methods in recent high-performance computer systems. The GPU is a specialized processor originally designed for graphics processing. Recently, the technologies such as CUDA which utilize the GPU resources more easily become popular, leading to the improved performance of the computer system by utilizing the CPU and GPU simultaneously in executing various kinds of applications. In this work, we analyze the temperature and the energy efficiency of the computer system where the CPU and the GPU are utilized simultaneously, to figure out the possible problems in upcoming high-performance computer systems. According to our experimentation results, the temperature of both CPU and GPU increase when the application is executed on the GPU. When the application is executed on the CPU, CPU temperature increases whereas GPU temperature remains unchanged. The computer system shows better energy efficiency by utilizing the GPU compared to the CPU, because the throughput of the GPU is much higher than that of the CPU. However, the temperature of the system tends to be increased more easily when the application is executed on the GPU, because the GPU consumes more power than the CPU.

Anti-Obesity Effect of Panax Ginseng in Animal Models: Study Protocol for a Systematic Review and Meta-Analysis (동물실험에서 인삼의 항비만 효과: 체계적 고찰과 메타분석을 위한 연구 프로토콜)

  • Cho, Jae-Heung;Kim, Koh-Woon;Park, Hye-Sung;Yoon, Ye-Ji;Song, Mi-Yeon
    • Journal of Korean Medicine for Obesity Research
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    • v.17 no.1
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    • pp.37-45
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    • 2017
  • Recently the global epidemic problem of obesity has stimulated intense interest in the study of physiological mechanisms using animal models as a way to gain crucial data required for translation to human studies. Panax ginseng has been reported to have anti-obesity or antidiabetic effects in many animal studies; however, there have been few studies investigating human obesity. Herein, we will assess and examine the evidence supporting the anti-obesity effect of Panax ginseng in animal models with respect to anthropometric and metabolic outcomes. We will include controlled, comparative studies assessing the effect of Panax ginseng in preclinical studies of obesity. Panax ginseng will be administered during or following the induction of experimental obesity. The primary outcome measure will be anthropometric assessment and the secondary outcome measures will include adipose tissue weight, total amount of food consumed and metabolic parameters. We will search MEDLINE, Embase, PubMed, Web of Science, and Scopus without language, publication date, or other restrictions. Ethical approval will not be necessary as the data collected in this study will not be individual patient data, consequently there will be no concerns about violations of privacy. After finishing the whole procedure, the results will be disseminated by publication in a peer-reviewed journal or presented at a relevant conference. This protocol has been registered on the Collaborative Approach to Meta-Analysis and Review of Animal Data from Experimental Studies (CAMARADES) website (http://www.camarades.info).

Effects of Induced Microcurrent Shoes on Fatigue and Pain in Painful Foot to Patients with Plantar Fascitis (족저근막염 환자의 미세전류발생 신발 착용이 족부 근피로 및 통증 완화에 미치는 영향)

  • Park, Rae-Joon;Choi, Sang-Joon;Cheng, Goh Ah;Cho, Jeong-Sun;Lee, Yoon-Mi;Cho, Yong-Ho;Park, So-Hyun;Cho, Mi-Suk
    • The Journal of Korean Physical Therapy
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    • v.18 no.1
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    • pp.1-10
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    • 2006
  • Purpose: This study was to investigate how induced microcurrent shoes affect relief of feet pain, which often occurs to persons who are more than fifty years old and how they prevent fatigue of feet generated during everyday life. The subjects of this study were comprised af five males and five females, who agreed with this research and ate more than fifty years old. And they all have plantar faciitis and pain in their feet. Methods: Subjects ware the induced microcurrent shoes far more than 4 hours everyday during the test. When they wore those shoes, they also wore the specially produced shacks made of silver-mixed thread and they were asked to avoid serious exercise. Prior to starting test and after the test, assessments were carried out two times. In the examination before the test, general shoes were used and in examination after the test, induced microcurrent shoes used. Muscle fatigue was measured by electromyography(MP150, Biopac, USA). When the walking rate of subjects on a treadmill was increased from 2.Km/h to 3Km/h for 10 minutes, muscle activities of a lower leg were measured during the whale walking process by MP150 remote monitoring system(TEL 100C). And pain was measured by VAS. Results: The results are as follows. 1. Comparisons of VAS before and after experimentation showed that degree of pain was significantly decreased(p < .05). 2. Degree of fatigue of Tibialis anterior was significantly decreased after 6 weeks of treatment(p < .05). 3. Degree of fatigue of Soleus was slightly decreased after 6 weeks of treatment, but did not show significant difference (p > .05). Conclusion: The above results suggest that induced microcurrent shoes is helpful decreasing fatigue of feet and relief of feet pain.

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Study on the Property of Guided Wave Signal Analysis according to Defect Shape of Small Size (소구경 튜브 결함 형태에 따른 유도초음파 신호 해석 특성에 관한 연구)

  • Gil, Doo-Song;Ahn, Yeon-Shik;Jung, Gye-Jo;Park, Sang-Gi;Kim, Yong-Gun
    • Journal of the Korean Society for Nondestructive Testing
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    • v.32 no.4
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    • pp.410-417
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    • 2012
  • Currently domestic thermal and nuclear power plants are comprised of many type's condenser and steam generator tubes to produce the electricity of good quality. There are some methods to inspect these tubes in the event that several defects were discovered in these facilities. Among many non-destructive methods, we used guided wave to inspect the soundness of tubes, because this method is very fast to detect the defect and very simple to install the equipment and also, can inspect up to the long range at a fixed point. Also, this method has a drawback that does not detect a very small size defect. So, we made an effort to overcome this drawback through the experimentation and signal analysis according to the size and shape of the defect through the manufacture of various artificial cracks capable to generate within the small size tube in the study and we anticipate that these detect limits can be overcome along with the development of the signal processing and manufacturing technology of the sensor for the inspection.

The nutritive value analysis of the mulberry cultured in Korea (한국에서 재배되는 상품종별 영양가치 분석)

  • 최병희;남중희
    • Journal of Sericultural and Entomological Science
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    • v.2
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    • pp.73-81
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    • 1962
  • This treatise is to analyze the chemical components in the mulberry varieties which are cultured in Korea and to estimate the nutritive values for them. Some of these varieties were developed lately in this country just by the general breeding aspect of mulberry leaf producing and silkworm raising experimentation without analysing the nutritive values of them which may be differed by various culturing condition. This work will be helpful for the improvement of mulberry varieties and the correct judgement of them. The varieties used for the analysis are as followings; A. Morus bombycis Koridz species. 1.Ichi-Hei. 2. Shimano-Uchi B. Morus alba L. species. 3. Kairyo-Nezumigaeshi. 4. Suwon-Daeyop. 5. Suwon No. 3. 6. Suwon No. 4. 7. Yongchon-Chuwoo. C. Morus Ihou (Ser.) Koidz species. 8. Ro-Soh. The specimens were eventually taken under the air and soil dry season because of the rain shortage during the sampling period and the results are found as a rather different from the normal specimen under the normal climate. Therefore, this treatise will be an important report on a special culturing condition and the nutritive values by the mulberry varieties, and still comparable because they were grown under the same condition. The individual conclusions are; 1. Yongchon-Chuwoo which was originated in this country, was found as an important variety for this country from the aspect of mulberry nutritive value and leaf producing amount, and it is believed that this is the best suitable variety for the soil of Korea. 2. Ichi-Hei occupies the better situation as well as Yongchon-Chuwoo from the nutritive criticizing even though it was originated in Japan. 3. The lately developed Suwon No.3 and No.4 are best from the leaf producing point of view, but they are not found such a good varieties from the nutritive aspect. The result may be happened as poor because they were cut before spring sprout started. 4. Ro-Soh which was normally recognized as a poor variety, was also found as a poor nutritive and leaf producing variety. 5. The crude protein and crude fat components in the mulberry leaves decreased as the leaf maturity was progressed, but the crude fiber and crude ash components increased reversively in general view. It was also found that there were some specific changing nature by the mulberry varieties and could not rule them by just one conclusion. 6. During the air and soil dry season, the ash component increases but the hydrocarbon and moisture contents decrease considerably, and which resulted to the increase of mulberry hardness ratio and the decrease of sugar-protein ratio, eventually it causes the decrease of the nutritive value. It was also found that Ichi-Hei, Ro-Soh, and Suwon No. 3 had a strong recovering nature to their normal nutritive condition after raining. 7. Mulberry is of course a rich calorie diet, and is calculated as 50 to 60 Cal. per gram.

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