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http://dx.doi.org/10.5352/JLS.2020.30.10.896

Probiotic Properties and Immunomodulator Evaluation of the Potential Feed Additive Pediococcus acidilactici SRCM102607  

Shin, Su-Jin (Microbial Institute for Fermentation Industry (MIFI))
Ha, Gwangsu (Microbial Institute for Fermentation Industry (MIFI))
Jeong, Su-Ji (Microbial Institute for Fermentation Industry (MIFI))
Ryu, Myeong Seon (Microbial Institute for Fermentation Industry (MIFI))
Kim, Jinwon (Microbial Institute for Fermentation Industry (MIFI))
Yang, Hee-Jong (Microbial Institute for Fermentation Industry (MIFI))
Kwak, Mi-Sun (Department of Bio and Fermentation Convergence Technology, Kookmin University)
Sung, Moon-Hee (Department of Bio and Fermentation Convergence Technology, Kookmin University)
Jeong, Do-Youn (Microbial Institute for Fermentation Industry (MIFI))
Publication Information
Journal of Life Science / v.30, no.10, 2020 , pp. 896-904 More about this Journal
Abstract
The purpose of this study was to investigate the probiotic characteristics and immune activities of selected lactic acid bacterial (LAB) strains as feed additives in livestock. 301 LAB strains isolated from traditional fermented foods were first assessed for their antibacterial activity potential. Of the 301 isolates, five showed antibacterial activity against five livestock pathogens (Esherichia coli KCCM11234, Listeria monocytogens KCTC3710, Salmonella Typhimurium KCTC1926, Staphylococcus aureus KCCM11593, and Shigella flexneri KCTC2517). The probiotic characteristics of the five selected strains were also investigated by antioxidative activity, hemolysis, bile salt hydrolase, acid resistance and bile tolerance. The SRCM102607 strain was found to have superior probiotic properties and was selected for further experimentation. 16S rRNA gene sequencing showed that SRCM102607 is Pediococcus acidilactici, which was labeled as P. acidilactici SRCM102607 (KCCM 12246P). The survival characteristics of P. acidilactici SRCM102607 in artificial gastrointestinal conditions were assessed under exposed acidic (pH 2.0) and bile (0.5% and 1.0%) conditions. P. acidilactici SRCM102607 was also confirmed to have resistance to various antibiotics, including amikacin, gentamicin, vancomycin, and etc. The TNF-α production by P. acidilactici SRCM102607 was 171.86±4.00 ng/ml. These results show that P. acidilactici RCM102607 has excellent potential for use as a probiotic livestock feed additive.
Keywords
Antibacterial activity; immune activity; Pediococcus acidilactici; probiotics; $TNF-{\alpha}$;
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1 Abbasiliasi, S., Tan, J. S., Bashokouh, F., Ibrahim, T. A. T., Mustafa, S., Vakhshiteh, F., Sivasamboo, S. and Ariff, A. B. 2017. In vitro assessment of Pediococcus acidilactici Kp10 for its potential use in the food industry. BMC Microbiol. 17, 121.   DOI
2 Argyri, A. A., Zoumpopoulou, G., Karatzas, K. A. G., Tsakalidou, E., Nychas, G. J. E., Panagou, E. Z. and Tassou, C. C. 2013. Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. Food Microbiol. 33, 282-291.   DOI
3 Blois, M. S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1200.   DOI
4 Calnek, B. W., Barnes, H. J., Beard, C. W., Reid, W. M. and Yoder, Jr. H. W. 1991. Salmonellosis-paratyphoid infections. In: Diseases of Poultry. pp. 99-130. 9th ed., Iowa State University Press Ames, IA, USA.
5 Capuron, L., Hauser, P., Hinze-Selch, D., Miller, A. H. and Neveu, P. J. 2002. Treatment of cytokine-induced depression. Brain Behav. Immun. 16, 575-580.   DOI
6 Chang, W. K., Cho, S. B., Kim, D. W., Lee, S. S. and Kim, S. K. 2010. Cell growth and antioxidant activity on onion juice fermentation by using Lactobacillus plantarum as animal probiotics. J. Life Sci. 20, 1729-1737.   DOI
7 Cho, Y. J., Choi, S. J., Kim, M. A., Kim, M., Yoon, S. J., Chang, M. I., Lee, S. M., Kim, H. J., Jeong, J., Rhee, G. S. and Lee, S. J. 2014. Simultaneous determination of aminoglycoside antibiotics in meat using liquid chromatography tandem mass spectrometry. J. Food Hyg. Safety 29, 123-130.   DOI
8 de Oliveira Vieira, K. C., Ferreira, C. D. S., Bueno, E. B. T., De Moraes, Y. A., Toledo, A. C. C. G., Nakagaki, W. R., Pereira, V. C. and Winkelstroter, L. K. 2020. Development and viability of probiotic orange juice supplemented by Pediococcus acidilactici CE51. Food Sci. Technol. 130, 1-7.
9 Dowarah, R., Verma, A. K., Agarwal, N. and Singh, P. 2018. Efficacy of species-specific probiotic Pediococcus acidilactici FT28 on blood biochemical profile, carcass traits and physicochemical properties of meat in fattening pigs. Res. Vet. Sci. 117, 60-64.   DOI
10 Dowarah, R., Verma, A. K., Agarwal, N., Patel, B. H. M. and Singh, P. 2017. Effect of swine based probiotic on performance, diarrhoea scores, intestinal microbiota and gut health of grower-finisher crossbred pigs. Livest. Sci. 195, 74-79.   DOI
11 Duncan, D. B. 1995. Multiple range and multiple F-test. Biometrics 2, 1-42.
12 Han, B. and Yang, S. B. 2019. The effects of food safety accident on the consumption of eggs: focusing on the pesticide- related accident. Kor. J. Org. Agric. 27, 17-32.   DOI
13 Kang, C. H., Han, S. H., Kim, Y. G., Jeong, Y. A. and Paek, N. S. 2017. Antimicrobial activity and probiotic properties of lactic acid bacteria isolated from traditional fermented foods. KSBB J. 32, 199-205.   DOI
14 Magaldi, S., Mata-Essayag, S., De Capriles, C. H., Perez, C., Colella, M. T., Olaizola, C. and Ontiveros, Y. 2004. Well diffusion for antifungal susceptibility testing. Int. J. Infect. Dis. 8, 39-45.   DOI
15 Kang, K. M. and Lee, S. H. 2012. Physiological characteristics of starter isolated form Kimchi and fermentation of Tofu with isolated starter. J. Kor. Soc. Food Sci. Nutr. 41, 1626-1631.   DOI
16 Kim, D. W., Cho, S. B., Jeong, H. Y., Moon, H. G., Lee, H. J., Hwang, B. J., Chung, W. T., Choi, C. W. and Chung, I. B. 2005. Activation of RAW 264.7 macrophage by digested bacterial cell of pig-derived Lactobacillus strains. J. Anim. Sci. Technol. 47, 947-954.   DOI
17 Ko, A. Y., Kim, H. J., Do, J. A., Jang, J., Lee, E. H., Ju, Y. J., Kim, J. Y., Chang, M. I. and Rhee, G. S. 2016. Development of analytical method for determination of spinetoram residues in livestock using LC-MS/MS. Anal. Sci. Technol. 29, 94-103.   DOI
18 Lee, E. Y. and Lim, J. S. 2011. Effects of the feed and probiotic feeding on the improvement of hoggery environment and the productivity of swine. Kor. J. Microbiol. Biotechnol. 39, 200-209.
19 Lee, S. H., Kim, Y. H., Kim, M. G., Kang, J. H. and Kang, D. J. 2015. Cholesterol lowering effect of Enterococcus faecium ID9201 isolated from breast milk-fed Korean infant. Curr. Top. Lactic Acid Bac. Probio. 3, 41-45   DOI
20 Mishra, V. and Prasad, D. N. 2005. Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int. J. Food Microbiol. 103, 109-115.   DOI
21 OECD, https://data.oecd.org/agroutput/meat-consumption.htm#indicator-chart.
22 Oh, S. T., Kang, C. W. and Kim, E. J. 2014. Effects of dietary supplementation of mixed probiotics on production performance and intestinal environment in broiler chicken. Kor. J. Poultry Sci. 41, 143-149.   DOI
23 Shin, J. S., Jung, J. Y., Lee, S. G., Shin, K. S., Rhee, Y. K., Lee, M. K., Hong, H. D. and Lee, K. T. 2016. Exopolysaccharide fraction from Pediococcus pentosaceus KFT 18 induces immunostimulatory activity in macrophages and immunosuppressed mice. J. Appl. Microbiol. 120, 1390-1402.   DOI
24 Park, J. S. and Choi, H. 2020. Simultaneous determination for fungicide prochloraz and its metabolites in animal commodities with GC-ECD after hydrolysis. J. Appl. Biol. Chem. 63, 153-159.   DOI
25 Park, S., Yoo, J., Seong, C. and Cho, S. 2013. Isolation and characterization of Bacillus subtilis MP56 with antimicrobial activity against MDR (multi drug resistant) strains. Kor. J. Microbiol. 49, 90-94.   DOI
26 Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
27 Seifert, H. S. and Gessler, F. 1996. Oral long-term administration of probiotic B. cereus an alternative for the the prevention of enterotoxemia. Dtsch Tierarztl Wochenschr. 103, 386-389.
28 Seo, J. W., Yang, H. J., Jeong, S. J., Ryu, M. S. and Jeong, D. Y. 2020. Potential probiotic activity of Lactobacillus brevis SCML 504 isolated from traditional Korean fermented food and the preparation of 'Sikhye' with brown rice. Kor. J. Food Preserv. 27, 46-57.   DOI
29 Shin, M. S., Kim, H. M., Kim, G. T., Huh, C. S., Bae, H. S. and Baek, Y. J. 1999. Selection and characteristics of Lactobacillus acidophilus isolated from Korean feces. Kor. J. Food Sci. Technol. 31, 495-501.
30 Song, H. J., Kim, K. J., Kim, H. D., Yoo, J. H., Koo, J. G. and Park, K. S. 2011. Probiotic properties of Pediococcus pentosaceus SH-10 isolated from the Hard Clam Meretrix meretrix Shikhae. Kor. J. Fish Aquat. Sci. 44, 605-611.   DOI
31 Song, K. O., Yang, H. S., Jung, S. K., Kang, W. C., Ko, J. A., Lee, C. H. and Kim, J. H. 2014. Encephalitic listeriosis in two Korean native goats in Jeju. KOJVS. 37, 73-78.
32 Yuksekdag, Z. N. and Aslim, B. 2010. Assessment of potential probiotic-and starter properties of Pediococcus spp. isolated from Turkish-type fermented sausages (sucuk). J. Microbiol. Biotechnol. 20, 161-168.   DOI
33 Taheri, H. R., Moravej, H., Malakzadegan, A., Tabandeh, F., Zaghari, M., Shivazad, M. and Adibmoradi, M. 2010. Efficacy of Pediococcus acidlactici-based probiotic on intestinal Coliforms and villus height, serum cholesterol level and performance of broiler chickens. Afr. J. Biotechnol. 9, 7564-7567.   DOI
34 Taranto, M. P., de Ruiz Holgado, A. P. and De Valdez, G. F. 1995. Bile salt hydrolase activity in Enterococcus faecium strains. Microbiol. Aliment. Nutr. 13, 375-379.
35 Wang, C., Wang, H., Zhao, Z., Xiao, S., Zhao, Y., Duan, C., Gao, L., Li, S. and Wang, J. 2019. Pediococcus acidilactici AS185 attenuates early atherosclerosis development through inhibition of lipid regulation and inflammation in rats. J. Funct. Foods 60, 103424.   DOI