• Title/Summary/Keyword: experimental techniques

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The Analysis of Volatile Components of Fresh Ginseng, Red Ginseng and White Ginseng by Solvent Free Solid Injector (SFSI) Techniques (Solvent Free Solid Injector (SFSI)를 이용한 수삼, 홍삼, 백삼의 향기성분 분석방법)

  • Kim, Mi-Ra;Kim, In-Hae;Shim, Jae-Han
    • Korean Journal of Environmental Agriculture
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    • v.24 no.2
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    • pp.164-168
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    • 2005
  • An experimental design has been used to optimize the analysis of volatile component from fresh ginseng using solvent free solid injector (SFSI). The analysis were performed at three different sample preparing methods (fresh, oven drying and freezed drying), different injector port temperatures (100, 150, 200, 250 and $300^{\circ}C$) and different preheating time (3, 5, 7, 10 and 15 min) according to the above experimental design. The optimum conditions from analytical results were fresh sample (sample preparing methods), $250^{\circ}C$ (injector temperature) and 10 min (preheating time). Ginsengs grown for six years at Gumsan were prepared for fresh, white and red ginsengs. These fresh, white and red singsengs were analyzed for their volatile components by GC/MS equipped with SFSI according to the above the optimum conditions. A total of thirty-three volatile components were identified in fresh ginseng by SFSI, thirty-six in white ginseng and thirty-eight in red ginseng. These results suggested that the SFSI method could be used for isolating volatile component in ginsengs.

AN EXPERIMENTAL STUDY OF ELECTROPHYSIOLOGICAL AND HISTOLOGICAL ASSESSMENT ON THE INJURY TYPES IN RABBIT INFERIOR ALVEOLAR NERVE (가토의 하치조 신경 손상 형태에 따른 전기생리학적 및 조직학적 변화에 관한 실험적 연구)

  • Lee, Jae-Eun;Lee, Dong-Keun
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.18 no.4
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    • pp.679-700
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    • 1996
  • Inferior alveolar nerve dysfunction may be the result of trauma, disease, or iatrogenic injury. Inferior alveolar nerve injury is inherent risk in endodontic therapy, orthognathic surgery of the mandible, and extraction of mandibular teeth, particularly the third molars. The sensory disturbances of inferior alveolar nerve associated with such injury have been well documented clinical problem that is commonly evaluated by several clinical sensory test including Tinels sign, Von Frey test(static light touch detection), directional discrimination, two-point discrimination, pin pressure nociceptive discrimination, and thermal test. These methods used to detect and assess inferior alveolar nerve injury have been subjective in nature, relying on the cooperation of the patients. In addition, many of these techniques are sensitive to differences in the examiners experience and skill with the particular technique. Data obtained at different times or by different examiners are therefore difficult to compare. Prior experimental studies have used electro diagnostic methods(sensory evoked potential) to objectively evaluate inferior alveolar nerve after nerve injury. This study was designed with inferior alveolar nerve of rabbit. Several types of injury including mind, moderate, severe compression and perforation with 19 gauze, 21 gauze needle and 6mm, 10mm traction were applied for taking the sesory evoked ppterntial. Latency and amplitude of injury rabbit inferior alveolar nerve were investigated with sensory evoked potential using unpaired t-test. The results were as follows : 1. Intensity of threshold (T1) was $128{\pm}16{\mu}A$ : latency, $0.87{\pm}0.07$ microsecond : amplitude, $0.4{\pm}0.1{\mu}V$ : conduction velocity, 23.3 m/s in sensory evoked potential of uninjured rabbit inferior alveolar nerve. 2. Rabbit inferior alveolar nerve consists of type II and III sensory nerve fiber. 3. Latency was increased and amplitude was decreased in compression injury. The more injured, the more changed in latency and amplitude. 4. Findings in perforation injury was similar to compression injury. Waveform for sensory evoked potential improved by increasing postinjured time. 5. Increasing latency was prominent in traction injury rabbit inferior alveolar nerve. 6. In microscopic histopathological findings, significant degeneration and disorganization of the internal architecture were seen in nerve facicle of severe compression and 10mm traction group. From the above findings, electrophysiological assessment(sensory evoked potential) of rabbit injured inferior alveolar nerve is reliable technique in diagnosis and prognosis of nerve injury.

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Strength Prediction Equations for High Strength Concrete by Schmidt Hammer Test (슈미트 해머 시험에 의한 고강도 콘크리트의 강도 추정식)

  • Kwon, Young-Wung;Park, Song-Chul;Kim, Min-Su
    • Journal of the Korea Concrete Institute
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    • v.18 no.3 s.93
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    • pp.389-395
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    • 2006
  • For the assessment of exsiting concrete structures, it is important to get the real strength of concrete. The load test or core test has many problems due to cost time, easiness, structural damage, and reliability and so on. Thus, various non-destructive test and statistical analysis techniques for strength assessment have been developed. As a result the real strength of concrete can be obtained by both direct and indirect test. In this study, a series of experimental tests of core strength and Schmidt hammer tests on 3, 7, 14, 28, 90, 180, 365, and 730 days' were done for predicting the compressive strength of high strength concrete with 65.0MPa of 28-days' strength. Each experimental results was analyzed by simple regression analysis. Then, reliability level and error rate between the proposed equations and the existing ones was examined. However, the application of the exsisting equations was inadequate to high strength concrete, because they were conducted under normal strength concrete. Therefore, the following compressive strength equations were proposed for predicting the compressive strength of high strength concrete by Schmidt hammer test. The proposed equations by Schmidt hammer test are as follows.

A study of Expression of TGF-β1, c-Myc, Erb-B2 and Thymosin-β4 Gene in Alcoholic Liver Damage Tissue. (알코올성 간 손상 조직에서 TGF-β1와 c-Myc, Erb-B2, Thymosin-β4 유전자 발현 융합 연구)

  • Kim, Jean-Soo;Choi, Sang-Ki
    • Journal of the Korea Convergence Society
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    • v.9 no.5
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    • pp.91-97
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    • 2018
  • This study has been conducted to see the expression of $TGF-{\beta}_1$, c-Myc, Erb-B2 and $Thymosin-{\beta}_4$ genes in ethanol - damaged liver tissues. Experimental groups were divided into 2 groups, one where damaged liver was caused by 25% ethanol and normal group administered with purified water. Results of test showed the expression of $TGF-{\beta}_1$, c-Myc, and $Thymosin-{\beta}_4$ genes was higher in the experimental group treated with 25% ethanol than in the normal group. Erb-B2 gene was not expressed clearly. Thus, it is considered that we can expect to utilize $TGF-{\beta}_1$, c-Myc 및 $Thymosin-{\beta}_4$ as auxiliary data and find clinical meanings of diagnosis on hepatic diseases, In addition to serologic and histological examination by convergence examining the gene expression status by molecular diagnostic techniques in liver-related disease prevention and diagnosis through results of this study.

The Early Growth Performances of Pinus densiflora and Larix kaempferi Seedlings Under Open-field Experimental Warming and Precipitation Manipulation (실외 실험적 온난화 및 강수 처리에 따른 소나무와 낙엽송 유묘의 초기 생장 특성)

  • Kwon, Boram;Cho, Min Seok;Yang, A-Ram;Chang, Hanna;An, Jiae;Son, Yowhan
    • Journal of Korean Society of Forest Science
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    • v.109 no.1
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    • pp.31-40
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    • 2020
  • This study aimed to investigate the effects of climate change on the survival and growth performance of Pinus densiflora and Larix kaempferi seedlings using open-field experimental warming and precipitation manipulation. We measured the survival rate, root-collar diameter, and height, and then calculated the seedling quality index (SQI) of 2-year-old seedlings under 6 treatments [2 temperatures (TC: Control; TW: Warming) × 3 precipitation manipulations (PC: Control; PD: Decreased; PI: Increased)] and performed a two-way ANOVA to test for differences.The air temperature of the warming plots was 3℃ higher than that of the control plots, while the precipitation manipulation plots received ±40% of the precipitation received by the control plots. Temperature and precipitation treatments did not significantly affect the survival rate of P. densiflora; however, the SQI of P. densiflora decreased with increasing precipitation. In contrast, the mortality rate of L. kaempferi increased with increasing temperature and decreasing precipitation. Furthermore, in L. kaempferi, TC × PI treatment resulted in the lowest SQI with a significant interaction effect observed between the two factors. In summary, low seedling production and quality should be expected in P. densiflora as precipitation increases and in L. kaempferi as temperature increases or precipitation decreases. These results indicate species-specific sensitivities to climate change of two plant species at the nursery stage. With the occurrence of global warming, the frequencies of drought and heavy rainfall events are increased, and this could affect the survival and seedling quality of tree species. Therefore, it is necessary to improve nursery techniques by establishing new adaptation strategies based on species-specific growth performance responses.

Application of Geophysical Techniques for Observing the Void Ratio Changes of Dredged Soils (준설토의 간극비 변화 관찰을 위한 물리탐사기법의 적용)

  • Hong, Young-Ho;Lee, Jong-Sub;Lee, Changho
    • Journal of the Korean Geotechnical Society
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    • v.30 no.9
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    • pp.19-28
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    • 2014
  • It is necessary to understand the behavior of the soils for the dredging constructions. The objective of this study is to estimate void ratio and density changes of the dredged soils by using the geophysical testing methods. A series of laboratory tests is performed to obtain geotechnical index properties of the specimen, retrieved from the west coastal of Korea. The sedimentation and self-weight consolidation tests are carried out with observing changes of the interfacial height and the elastic wave velocities. The same amounts of the soils are poured into the testing column at intervals of 12 hours until the interheight reaches to a certain level. After the completion of the sedimentatation and self-weight consolidation tests, downward permeability test is performed to assess a tidal influence in the nearshore. The mini resistance cone is penetrated into the specimen to measure the electrical resistivity with depth. All tests are completely finished, the weight of specimens are measured to calculate the void ratio with the depth. Experimental results show that the aspects of the self-weight consolidation are invisible during dredging process because of rapid sedimentation characteristics of ML. However, the elastic wave velocities increase with increasing in the effective stresses. During permeability test, measured permeability and the elastic wave velocities maintain almost identical values. Void ratio based on the elastic wave velocities changes linearly with time during the step dumpings. Void ratio estimated by the electrical resistivity represents the repeatedly layered depositions according to the step-by-step dumpings. Void ratio determined by soil sampling is similar to those of elastic waves and electrical resistivity profiles. This experimental study demonstrates that the geophysical testing methods may be an effective method for evaluating the behavior of dredged soils.

BOND STRENGTH OF RESIN CEMENTS TO ZIRCONIA CERAMIC (지르코니아 세라믹과 레진 시멘트의 결합강도)

  • Chang Mun-Suk;Kim Ji-Hye;Cho Suck-Kyu;Bok Won-Mi;Song Kwang-Yeob;Park Ju-Mi
    • The Journal of Korean Academy of Prosthodontics
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    • v.43 no.4
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    • pp.426-437
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    • 2005
  • Statement of problem : Although zirconium oxide ceramics are more and more commonly used in restorative dentistry, for many clinical applications only limited data can be found in the literature. However it is quite clear that hydrofluoric acid etching is impossible with zirconia ceramics. Therefore, other bonding techniques are required in order to lute these materials adhesively. Purpose : The purpose or this study was to evaluate the effects of surface treatments on shear bond strengths between two resin cements and a zirconia ceramic. Materials and methods : Experimental industrially manufactured yttrium-oxide-partially-stabilized zirconia ceramic discs (Adens, Korea) were used for this study. The ceramic specimens divided into five experimental groups and a control group (as received). Five surface treatments were studied 1) sandblasting with 110$\mu$m $Al_2O_3$ at 3 bars pressure 13 seconds at a distance of 10 mm, 2) flame-treated with the Silano-Pen for 5 $s/cm^3$, 3) grinding with a diamond bur. 4) sandblasting + Silano-Pen treatment, 5) diamond bur preparation + Silano-Pen treatment. Acrylic plastic tube (5 mm in height and 3 mm in diameter) were filled with composite to fabricate composite cylinders The composite cylinders were bonded to the ceramic specimens with either Superbond C&B or Panavia F resin luting agents. All cemented specimens were tested under shear loading until fracture on universal testing machine at a crosshead speed 1mm/min; the maximum load at fracture was recorded. Sheat bond strength data were analyzed with oneway analysis of variance and Tukey HSD tests (P<.05). Treated ceramic surfaces and fracture surfaces after shear testing were examined morphologically using scanning electron microscope. Results: Ceramic surface treatment with Silano-Pen after sandblasting improved the bond strength of Superbond C&B resin cement. Supevbond C& B resin cement at Silano-Pen aiker sandblasting($27.4{\pm}3.8MPa$) showed statistically higher shear bond strength than the others. Conclusion: Within the limitation of this study, Superbond C& &B resin cement are suitable for cementation of zirconia ceramics and flame-treated with the Silano-Pen after sandblasting is required to enhance the bond strength.

An Experimental Study to Evaluate Hydraulic Characteristics and Stability of Scour Protection for Historic Site Restoration of Woljeong-gyo (월정교 사적지 복원을 위한 수리특성 및 세굴보호공 안정성 검토에 관한 수리모형실험 연구)

  • Kang, Joon-Gu;Yeo, Hong-Koo;Son, Byung-Ju;Ji, Un
    • Journal of Korea Water Resources Association
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    • v.41 no.8
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    • pp.845-854
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    • 2008
  • The old bridge of Woljeong-gyo, which has the fluvial history and culture, represents the ancient construction and civil engineering techniques. It is the oldest stone bridge in Korea and currently restored with its vicinities. In this study, the experimental model was used to analyze the hydraulic characteristics, the local pier-scour depth without scour protection, and the stability of riprap protection using the old grid type panels and stones for Woljeong-gyo of the study area. The water levels were increased around 30cm due to the restored bridge piers and foundations and the effects went up to 200m upstream. The maximum scour depth of 5.4m was measured and the scour protection tests were performed with the riprap size calculated using empirical equations and the existing scour protection range. The riprap of the existing scour protection in the upstream side was broken away, while the riprap of extended scour protection was very stable for the design flood condition of Woljeong-gyo area.

Splitting of Surface Plasmon Resonance Peaks Under TE- and TM-polarized Illumination

  • Yoon, Su-Jin;Hwang, Jeongwoo;Lee, Myeong-Ju;Kang, Sang-Woo;Kim, Jong-Su;Ku, Zahyun;Urbas, Augustine;Lee, Sang Jun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.296-296
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    • 2014
  • We investigate experimentally and theoretically the splitting of surface plasmon (SP) resonance peaks under TE- and TM-polarized illumination. The SP structure at infrared wavelength is fabricated with a 2-dimensional square periodic array of circular holes penetrating through Au (gold) film. In brief, the processing steps to fabricate the SP structure are as follows. (i) A standard optical lithography was performed to produce to a periodic array of photoresist (PR) circular cylinders. (ii) After the PR pattern, e-beam evaporation was used to deposit a 50-nm thick layer of Au. (iii) A lift-off processing with acetone to remove the PR layer, leading to final structure (pitch, $p=2.2{\mu}m$; aperture size, $d=1.1{\mu}m$) as shown in Fig. 1(a). The transmission is measured using a Nicolet Fourier-transform infrared spectroscopy (FTIR) at the incident angle from $0^{\circ}$ to $36^{\circ}$ with a step of $4^{\circ}$ both in TE and TM polarization. Measured first and second order SP resonances at interface between Au and GaAs exhibit the splitting into two branches under TM-polarized light as shown in Fig. 1(b). However, as the incidence angle under TE polarization is increased, the $1^{st}$ order SP resonance peak blue-shifts slightly while the splitting of $2^{nd}$ order SP resonance peak tends to be larger (not shown here). For the purpose of understanding our experimental results qualitatively, SP resonance peak wavelengths can be calculated from momentum matching condition (black circle depicted in Fig. 2(b)), $k_{sp}=k_{\parallel}{\pm}iG_x{\pm}jG_y$, where $k_{sp}$ is the SP wavevector, $k_{\parallel}$ is the in-plane component of incident light wavevector, i and j are SP coupling order, and G is the grating momentum wavevector. Moreover, for better understanding we performed 3D full field electromagnetic simulations of SP structure using a finite integration technique (CST Microwave Studio). Fig. 1(b) shows an excellent agreement between the experimental, calculated and CST-simulated splitting of SP resonance peaks with various incidence angles under TM-polarized illumination (TE results are not shown here). The simulated z-component electric field (Ez) distribution at incident angle, $4^{\circ}$ and $16^{\circ}$ under TM polarization and at the corresponding SP resonance wavelength is shown in Fig. 1(c). The analysis and comparison of theoretical results with experiment indicates a good agreement of the splitting behavior of the surface plasmon resonance modes at oblique incidence both in TE and TM polarization.

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Reflection Signal Analysis for Time Division Multiplexing of Fiber Optic FBG Sensors (광섬유 FBG 센서의 시간 분할 다중화를 위한 반사 신호의 분석)

  • Kim, Geun-Jin;Kwon, Il-Bum;Yoon, Dong-Jin;Hwang, Du-Sun;Chung, Young-Joo
    • Journal of the Korean Society for Nondestructive Testing
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    • v.30 no.1
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    • pp.6-12
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    • 2010
  • Fiber optic sensor using fiber Bragg grating(FBG) probes is used for monitoring strain and temperature distributed on the wide surfaces of large structures. In this paper, in order to use many FBG probes in one optical fiber line, we propose a complex multiplexing technology which is composed of two techniques, one is time division multiplexing and another is wavelength division multiplexing. However, we only investigate the characteristics of time division multiplexing because FBG sensors basically can be operated by wavelength division multiplexing. We calculate the optimal reflectivities and the lengthwise location of five FBG probes in serial connection in order to obtain the unique reflected intensities from the FBG probes. We fabricate five FBG probes with the reflectivities of 13%, 16%, 25%, 40% and 80%, which are determined by the theoretical calculation, and observe the signal reflected from each FBG in the time domain from the experiment. There are differences between experimental and theoretical results caused by the signal noise and the differences of reflectivities of FBG probes. But the experimental results shows the reflected signals of five FBG probes which prove the availability of complex multiplexing.