Nath, Caroline Daiane;Neres, Marcela Abbado;Scheidt, Kacia Carine;Bersot, Luciano dos Santos;Sunahara, Samantha Mariana Monteiro;Sarto, Jaqueline Rocha Wobeto;Stangarlin, Jose Renato;Gomes, Simone Damasceno;Sereno, Mallu Jagnow;Perin, Ana Paula
Asian-Australasian Journal of Animal Sciences
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v.31
no.8
/
pp.1197-1204
/
2018
Objective: The objective was to characterize the fermentative and microbiological profile of Tifton 85 bermudagrass haylage with different layers of polyethylene film and storage time. Methods: The experimental design consisted of a randomized block design with four and six wrapping layers (100 and 150 microns in total. respectively) allocated in the main plots, through repeated measures analysis (30, 60, and 90 days of storage) with four replicates. Results: The storage time and number of wrapping layers did not show changes in the population of Clostridium and lactic acid bacteria. A decrease was observed in the enterobacteria population with an increase in the storage period in the two wrapping layers studied. Upon opening of the haylage at 30 days, the population of Bacillus was lower in haylages made with six layers of wrapping (3.63 log colony forming units/g). No growth of Listeria sp. or Salmonella sp. was observed during the experimental period. The fungal genera with a greater occurrence were Penicillium sp. and Fusarium sp. The following mycotoxins were not detected: ochratoxin A, fumonisins, and zearalenone. Relative to the organic butyric, propionic, and acetic acids, the haylages presented a low concentration of lactic acid; this may have prevented a drop in the pH, which was high when the silos were opened (5.4). The levels of ammoniacal nitrogen and soluble carbohydrates presented no variation among the number of wrapping layers, with an overall average of 35.55 and 38.04 g/kg. Conclusion: Tifton 85 bermudagrass haylage wrapped with four and six layers presented adequate fermentation and microbiological characteristics in the evaluated periods.
Han Kyung-Ja;Kim Hyun-Ah;Kim Sook-Young;Kim Jeong-Soo
Child Health Nursing Research
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v.8
no.4
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pp.449-457
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2002
The main purpose of this study is to examine the concepts appeared on researches and provide the future research directions in field of child health nursing. A descriptive study was conducted. 205 studies were included in an analysis the concepts in terms of the domain of client for a total 321 researches. All were originally published between 1990 and 2000 in Korea. An analysis of concepts for this study was used the metaparadigm framework for nursing proposed by H.S. Kim(2000). The concepts for this study were categorized by essentialistic concepts, problematic concepts, health-care experiential concepts with the following results. 1. Based on the four domains suggested Kim (2000), 205 studies(63.9%) belong to the domain of client ; 109 studies(34%) belong to the practice domain of nursing; 3 studies(0.9%) belong to the client-nurse domain; and 4 studies belong to the domain of environment, respectively. 2. In the domain of client, 117(57.1%) studies used concept of parent. Among them, mother was the prevailing research population(103 studies). 64 studies(31.2%) used child population and the developmental stage of children varied from preterm to puberty but school aged children was the most target population(28 studies). Family as a concept of client was used in 20 studies(9.8%) but most primary care provider was the mother. 3. In terms of research design, non-experimental design(83.5%) is the most and among them survey was 159 studies(77.6%). Qualitative research(23 studies) and experimental research(10 studies) methods were used relatively few. 4. In terms of the categories of concepts, 196 (61.4%) studies included the essentialistic concepts like stress and coping(20.4%), mothering role and child care(7.8%), health (5.6%), breast feeding(4.7%). 31 studies included problematic concepts like power-lessness, safety, obesity, pain, anxiety. And 65 studies included health-care experiential concepts like compliance, growth, hope, environment but relatively few. The findings of this study provide the evidence that research related problematic concept and health-care experiential concept should be conducted actively to improve the practice of child health nursing. Also to deeply understand the phenomena of client in field of child health nursing, interpretive research methods should be conducted actively, too.
Kim, H.S.;Ryu, B.Y.;Oh, S.K.;Suh, C.S.;Kim, S.H.;Choi, Y.M.;Kim, J.G.;Moon, S.Y.;Lee, J.Y.
Clinical and Experimental Reproductive Medicine
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v.25
no.1
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pp.59-64
/
1998
This study was designed to evaluate the influence of pronuclear age on the survival and post-thawing development after cryopreservation of mouse embryos. Freezing and thawing were performed in the different pronuclear stages of mouse embryos after IVF. Embryos were obtained from $F_1$ hybrid mice and classified into 4 groups according to the pronuclear stage (6hr, 9hr, 12hr and 15hr after insemination). Pronuclear ova were slowly cooled in a biological freezer using 1.5M 1,2-propanediol and 0.1M sucrose as cryoprotectant. Thawing was done at room temperature and 1,2-propanediol was removed by multi-step dilutions. Both frozen-thawed embryos and control fresh embryos were cultured in vitro in Ham's F-10 medium supplemented with 4mg/ml BSA. In control group, the development rate after 48hr was 99.3%, and the complete hatching rate after 144hr was 61.3%. In experimental groups, the survival rate after thawing was 95.4% in 6hr, 88.7% in 9hr, 75.2% in 12hr and 62.4% in 15hr after insemination, the development rate after 48hr was 61.1, 77.0, 67.0 and 79.6%, respectively, and the complete hatching rate after 144hr was 25.7, 43.7, 42.2 and 60.0%, respectively. The survival rate in 15hr was significantly lower (p<0.05) compared with other groups. In vitro development rates after 48hr were similar in all groups, but complement hatching rate was significantly lower (p<0.05) in 6hr group. In conclusion, cryopreservation of mouse pronuclear ova with 2 distinct pronuclei (9hr and 12hr groups) showed better results after thawing compared with early (6hr group) or late pronuclear ova just prior to cleavage (15hr group).
The purpose of this study was to examine the effects of hand massage as a nursing intervention on the anxiety of the hysterectomy patients in immediately prior to surgery. The method of this study was Nonequivalent Control Group Non-Synchronized Design. The data were selected from at K university hospital in Pusan, and they consisted of Experimental group-25 patients, Control group -24 patients. The data were collected from Jan. 4 to Jan. 30 in 1999. The subjects′ self-reports of anxiety (measured by the Spielberger Trait-State anxiety Inventory and Visual Analogue Scale developed by Cline et al.) were recorded before and immediately after the intervention. The objective physiologic measures of blood pressure and pulse rate. The collected data were analysed by means of frequency, percentage, standard deviation, chi- square test, t-test, ANCOVA with SPSS program. The results of this study were as following; 1. Hypothesis 1: The 1st hypothesis that "There will be significant difference of the state anxiety level just before surgery in the experimental group and control group" was supported(P= .000). 2. Hypothesis 2: The 2nd hypothesis that "There will be significant difference of the visual analogue scale score just before surgery in the experimental group and control group"was supported(P= .000). 3. Hypothesis 3: The 3rd hypothesis that "There will be significant difference of the systolic and diastolic blood pressure level just before surgery in the experimental group and control group"was supported (P= .003, P= .041). 4. Hypothesis 4: The 4th hypothesis that "There will be significant difference of the pulse rate just before surgery in the experimental group and control group"was supported(P= .004). In conclusion, hand massage is a benefical nursing intervention that alleviates the psychological, physiological anxiety of the hysterectomy patients in immediately prior to surgery. therefore it is recomended to use the hand massage as a nursing intervention for patients undergoing anxiety. The results of this study appear promising, additional research is recomended to further the appropriate uses of hand massage in nursing practice for this and other patient population.
Choi, Jung Hoon;Kim, Ju Ae;Hong, Nam Soon;LEE, Bo Hye;LEE, Eun Hee
Journal of Korean Clinical Nursing Research
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v.19
no.3
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pp.383-394
/
2013
Purpose: This study was conducted to investigate the effects of preparatory nursing information and information offering by SMS on anxiety and nursing satisfaction among family members of ICU (Intensive Care Unit) inpatients. It utilized a quasi-experimental research design with a non-equivalent and non-synchronized control group. Methods: The experimental group and the control group were offered a booklet guiding ICU and were offered face-to-face information by nurses using Power Point. The experimental group was additionally offered SMS (short message service) message on patient's safety at night every morning during the hospital stay between 8 and 9 am. All participants in the experimental group and the control group were measured in reference to anxiety at the point of hospitalization and were surveyed anxiety and nursing satisfaction on the third day of hospital stay. Results: The anxiety demonstrated no significant difference between the groups, while the nursing satisfaction was significantly higher in the experimental group than that of the control group. Conclusion: Proving direct information and indirect information via SMS message were considered effective in improving the family member's satisfaction with nursing care. The frequencies of SMS message and the contents of the message and information need to be defined and specified in the given population.
Purpose: This study was conducted to examine the effect of foot-bath on lower extremities edema and fatigue among college students in Korea. Methods: This study used an quasi-experimental design with 56 participants (30 for experiments and 26 for controls). The experimental group performed 20-minute foot bath before sleep three times for one week. All subjects were directed to use their smart-phones to fill out a questionnaire about fatigue. Leg circumference was measured 10 minutes after foot bath. The test was performed from April 30th to May 22th in 2016. Data was analyzed using t-test and $x^2$ test. Results: We found no difference in general characteristics between the experimental group and the control group. Left leg edema of the experimental group decreased by $16.63{\pm}14.57mm$ (p<.001). The experimental group's right leg-edema decreased by $13.10{\pm}13.97mm$ (p<.001). There was no statistically significant difference in their fatigue level when comparing before and after the foot baths. Conclusion: We found that doing foot baths for two weeks could have positive effects in reducing leg-edema among college students. Foot bath may be applied as an effective nursing intervention to decrease leg edema among young people. The results are based on a limited number of study samples and a short-term intervention. Further research can be performed with extended population and a prolonged study period.
This study was conducted to investigate the effects of Momordica charantia saponin (MCS) on ruminal fermentation of maize stover and abundance of selected microbial populations in vitro. Five levels of MCS supplements (0, 0.01, 0.06, 0.30, 0.60 mg/mL) were tested. The pH, $NH_3-N$, and volatile fatty acid were measured at 6, 24, 48 h of in vitro mixed incubation fluids, whilst the selected microbial populations were determined at 6 and 24 h. The high dose of MCS increased the initial fractional rate of degradation at t-value = 0 ($FRD_0$) and the fractional rate of gas production (k), but decreased the theoretical maximum of gas production ($V_F$) and the half-life ($t_{0.5}$) compared with the control. The $NH_3-N$ concentration reached the lowest concentration with 0.01 mg MCS/mL at 6 h. The MSC inclusion increased (p<0.001) the molar proportion of butyrate, isovalerate at 24 h and 48 h, and the molar proportion of acetate at 24 h, but then decreased (p<0.05) them at 48 h. The molar proportion of valerate was increased (p<0.05) at 24 h. The acetate to propionate ratio (A/P; linear, p<0.01) was increased at 24 h, but reached the least value at the level of 0.30 mg/mL MCS. The MCS inclusion decreased (p<0.05) the molar proportion of propionate at 24 h and then increased it at 48 h. The concentration of total volatile fatty acid was decreased (p<0.001) at 24 h, but reached the greatest concentration at the level of 0.01 mg/mL and the least concentration at the level of 0.60 mg/mL. The relative abundance of Ruminococcus albus was increased at 6 h and 24 h, and the relative abundance of Fibrobacter succinogenes was the lowest (p<0.05) at 0.60 mg/mL at 6 h and 24 h. The relative abundance of Butyrivibrio fibrisolvens and fungus reached the greatest value (p<0.05) at low doses of MCS inclusion and the least value (p<0.05) at 0.60 mg/mL at 24 h. The present results demonstrates that a high level of MCS quickly inhibits in vitro fermentation of maize stover, while MCS at low doses has the ability to modulate the ruminal fermentation pattern by regulating the number of functional rumen microbes including cellulolytic bacteria and fungi populations, and may have potential as a feed additive applied in the diets of ruminants.
Kim, Soo-Kyung;Lee, Jung-Eun;Youn, Mee-Kyung;Ko, Hee-Jeong
Journal of the Korea Academia-Industrial cooperation Society
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v.15
no.3
/
pp.1448-1457
/
2014
Geriatric illness is increasing with aged population ratio due to a prolonged average life span. Research studies with therapeutic approach using oriental herbs have been attempted, but the very few studies of oriental herbal tea as a healthy drink have done as a health care. This study was performed to identify the effects of oriental herbal tea on the physiological function and behavioral change of elders at geriatric facility. A quasi-experimental design using a nonequivalent control group, pre-post test was used. Total 38 of elderly population (20 of experimental group and 18 of control group) was recruited. 100mL of a type of oriental herbal tea developed for this study was given to each subject 3 times a day for 30 days (from May to June 2013). The health scan analyzer and questionnaire for behavioral change were used to measure dependent variables. The data was analyzed by SPSS/WIN 18.0. After drinking the oriental herbal tea, more significant improvement on blood circulation (t=-4.466, p<.001), liver function (t=-2.855, p=.007), brain function (t=-2.545, p=.015), bone density (t=-6.125, p<.001), blood sugar (t=-4.180, p=<.001) and emotional factor (t=2.389, p=.023) as the sub-variable of behavioral change were found in the experimental group than control group. This study shows that oriental herbal tea can be effective on the physiological function and emotional relief of the elders. Therefore the tea can be utilized as an effective intervention for the health of elders in geriatric facility.
The groundwater has been a major source of water supply throughout the ages. Around 50% of the rural as well as urban population in the developing countries like India depends on groundwater for drinking. The groundwater is also an important source in the agriculture and industrial sector. In many parts of the world, groundwater resources are under increasing threat from growing demands, wasteful use and contamination. A good planning and management practices are needed to face this challenge. A key to the management of groundwater is the ability to model the movement of fluids and contaminants in the subsurface environment. It is obvious that the contaminant source activities cannot be completely eliminated and perhaps our water bodies will continue to serve as receptors of vast quantities of waste. In such a scenario, the goal of water quality protection efforts must necessarily be the control and management of these sources to ensure that released pollutants will be sufficiently attenuated within the region of interest and the quality of water at points of withdrawal is not impaired. In order to understand the behaviour of contaminant transport through different types of media, several researchers are carrying out experimental investigations through laboratory and field studies. Many of them are working on the analytical and numerical studies to simulate the movement of contaminants in soil and groundwater of the contaminant transport. With the advent of high power computers especially, a numerical modelling has gained popularity and is indeed of particular relevance in this regard. This paper provides the state of the art of contaminant transport and reviews the allied research works carried out through experimental investigation or using the analytical solution and numerical method. The review involves the investigation in respect of both, saturated and unsaturated, porous media.
Purpose: This study was to determine the effects of a combined exercise program and telephone counseling in obese middle-aged women. The study also attempted to measure the effects of the program on physiological functions, self-efficacy and depression. Method: This research employed a nonequivalent control group pretest-posttest design. The experimental group consisted of 29 obese middle-aged women, and the control group of 30 women. The subjects in the experimental group participated in a combined exercises program and telephone counseling for 12 weeks. Results: After performing the program for 12 weeks, BMI (p=.000), percent body fat (p<.007), TC (p=.026), TG (p=.035) and depression (p=.012) in the experimental group decreased significantly compared with the control group. Self-efficacy (p=.000) in the experimental group increased significantly compared with the control group after the program. Conclusion: From these findings, it was confirmed that a combined exercises program and telephone counseling for obese middle-aged women can increase self-efficacy and decrease BMI, percent body fat, total cholesterol, triglyceride and depression. Therefore, this program can be utilized for various population groups including women, and further researches are required on programs for men.
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