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Evaluation of Spinal Fusion Using Bone Marrow Derived Mesenchymal Stem Cells with or without Fibroblast Growth Factor-4

  • Seo, Hyun-Sung;Jung, Jong-Kwon;Lim, Mi-Hyun;Hyun, Dong-Keun;Oh, Nam-Sik;Yoon, Seung-Hwan
    • Journal of Korean Neurosurgical Society
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    • v.46 no.4
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    • pp.397-402
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    • 2009
  • Objective : In this study, the authors assessed the ability of rat bone marrow derived mesenchymal stem cells (BMDMSCs), in the presence of a growth factor, fibroblast growth factor-4 (FGF-4) and hydroxyapatite, to act as a scaffold for posterolateral spinal fusion in a rat model. Methods : Using a rat posterolateral spine fusion model. the experimental study comprised 3 groups. Group 1 was composed of 6 animals that were implanted with 0.08 gram hydroxyapatite only. Group 2 was composed of 6 animals that were implanted with 0.08 gram hydroxyapatite containing $1{\times}10^6/60{\mu}L$ rat of BMDMSCs. Group 3 was composed of 6 animals that were implanted with 0.08 gram hydroxyapatite containing $1{\times}10^6/60{\mu}L$ of rat BMDMSCs and FGF-4 $1{\mu}G$ to induce the bony differentiation of the BMDMSCs. Rats were assessed using radiographs obtained at 4, 6, and 8 weeks postoperatively. After sacrifice, spines were explanted and assessed by manual palpation, high-resolution microcomputerized tomography, and histological analysis. Results : Radiographic, high-resolution microcomputerized tomographic, and manual palpation revealed spinal fusion in five rats (83%) in Group 2 at 8 weeks. However, in Group 1, three (60%) rats developed fusion at L4-L5 by radiography and two (40%) by manual palpation in radiographic examination. In addition, in Group 3, bone fusion was observed in only 50% of rats by manual palpation and radiographic examination at this time. Conclusion : The present study demonstrates that 0.08 gram of hydroxyapatite with $1{\times}10^6/60{\mu}L$ rat of BMDMSCs induced bone fusion. FGF4, added to differentiate primitive $1{\times}10^6/60{\mu}L$ rat of BMDMSCs did not induce fusion. Based on histologic data, FGF-4 appears to induce fibrotic change rather than differentiation to bone by $1{\times}10^6/60{\mu}L$ rat of BMDMSCs.

The Effect of Backward Pedaling Ergometer Training on Ankle ROM, Lower Extremities Strength, Foot Pressure in Hemiplegia (역방향 에르고미터 훈련이 편마비 환자의 족관절 가동범위와 하지 근력 및 족저압에 미치는 영향)

  • Ha, Mi-Seon;Kim, Eun-Jung;Kim, Myeong-Hee;Oh, Tae-Young
    • Journal of the Korean Society of Physical Medicine
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    • v.8 no.3
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    • pp.467-477
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    • 2013
  • PURPOSE: The purpose of this study was to identify the effects of backward pedaling ergometer training on ankle ROM, lower extremities strength, foot pressure in hemiplegia. METHODS: The subjects consisted of control group(n=10) and experimental group(n=10), subjects were trained 5 times a week for 2 weeks each group. Control group were trained forward, experimental group were trained backward pedaling with physical therapy in both groups. Each group measured ankle joint ROM with DUALER IQ and ankle and knee joint flexor and extensor muscle strength by Manual Muscle Test System and foot pressure by Gait view system. RESULTS: The result of this study between pre and post test that experimental group had statistically significantly differences in ankle joint range of motion and lower extremities strength. But foot pressure had not statistically significant differences. There was not significantly difference of variation between groups. CONCLUSION: Therefore these results mean that backward and forward pedaling ergometer training effected an improvement of lower extremities function in hemiplegia.

A Study on Changes in Learners' Recognition of 21st Century Learner Competencies through Science-centered STEAM Class (과학중심 STEAM 수업을 통한 학습자가 인식하는 21세기 학습자 역량 변화)

  • Shim, Su-min;Shin, Youngjoon
    • Journal of Korean Elementary Science Education
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    • v.36 no.2
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    • pp.143-154
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    • 2017
  • The purpose of this study is to examine the educational effects of STEAM class by focusing on $21^{st}$ century learner competencies based on burning and extinguishing unit. Two 6th grade classes were divided into experimental group and comparison group to treat the experimental group with elementary science class using STEAM class. General class according to teacher manual was implemented for the comparison group. Elementary science class applying STEAM class was conducted for 9 lessons throughout the experimental period. The result of this study are as follows. Elementary science class with STEAM class had significant effect on 21st century learner competencies compared to traditional instructor-led classroom. Looking specifically, it was a significant difference in the three domains of the seven domains (problem solving, collaboration, flexibility). The study results indicated that STEAM class was effective in improving learners'recognition of 21st century learner competencies.

The Effect of Elementary Science Class with Name Card Method on Learning Motivation and Academic Achievement of Elementary Students (Name Card 기법을 적용한 초등과학 수업이 초등학생의 과학 학습 동기 및 학업성취도에 미치는 영향)

  • Yang, Seung-Won;Bae, Jinho;So, Keum-Hyun
    • Journal of Korean Elementary Science Education
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    • v.33 no.1
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    • pp.129-139
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    • 2014
  • This study was conducted to examine the effect of elementary science class using name card method on scientific learning motivation and academic achievement of elementary students. Two sixth grade classes were divided into experimental group and comparison group to treat the experimental group with elementary science class using name card method. General class according to teacher manual was implemented for the comparison group. Elementary science class applying name card method was conducted for 10 sessions throughout the experimental period of 8 weeks. The results of this study were as follows. First, elementary science class with name card method was effective in improving scientific learning motivation. Second, elementary science class with name card method had significant effect on improvement of scientific learning academic achievement. The study results showed that elementary science class with name card method was effective for scientific learning motivation and academic achievement of elementary students.

Experimental investigation on shear capacity of RC beams with GFRP rebar & stirrups

  • Vora, Tarak P.;Shah, Bharat J.
    • Steel and Composite Structures
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    • v.21 no.6
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    • pp.1265-1285
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    • 2016
  • This paper presents experimental results of advanced investigation carried out on the beams reinforced with Glass Fiber Reinforced Polymer (GFRP) rebar and stirrups. Twelve beams reinforced with GFRP and one beam with steel reinforcement of size $230{\times}300{\times}2000mm$ were investigated. Longitudinal reinforcement, shear span and spacing of stirrups were the main variables to form the set. In advanced testing three types of strain gauges for steel, composite and concrete surface were applied to observe strain/stress development against the applied load. Live data were recorded from four strain gauges applied on stirrups, one at center on longitudinal reinforcement, two on the concrete surface and central deflection during the test. Although the focus of the paper was mainly on the behavior of GFRP shear reinforcement, other parallel data were observed for the completeness of the test. Design recommendations of ISIS Canada Design Manual (2007), Japan Society of Civil Engineers (1997) and American Concrete Institute (ACI-440.1R-06) were reviewed. Shear design predictions were compared with experimental results in which it was observed that all the three standards provided conservative predictions. However, ACI found most efficient compare to other two there is room to improve the efficiency of the recommendations.

The Effects of Passive Exercise on Upper Extremities Muscle Spasticity, Finger Edema and Depression for Chronic Stroke Patients at Long-term Care Hospitals (상지수동운동이 만성 뇌졸중 노인 환자의 상지경직, 손가락부종 및 우울에 미치는 효과)

  • Nam, Song Mi;Eun, Young
    • Journal of muscle and joint health
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    • v.25 no.3
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    • pp.167-175
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    • 2018
  • Purpose: The aim of this study was to test the effects of passive exercise on upper extremities muscle spasticity, finger edema, and depression for chronic stroke patients. Methods: A non-equivalent pretest-posttest design was employed for this study. The subjects were 30 elderly patients who were hospitalized to treat chronic stroke. 15 patients were assigned to the experimental and control groups respectively. Passive exercise for 20 minutes per day, five days a week for 8 weeks (total 40 times) was provided for the experimental group. Outcome measurements included manual spasticity test to measure upper extremities' muscle spasticity, the ring measurement method for finger edema and the Korean version of Short-form Geriatric Depression Scale (SGDS-K) for depression. Results: The upper extremities' muscle spasticity (Z=-2.52 p=.012) and the degree of depression (F=5.56, p=.006) in the experimental group were significantly reduced compared to those of the control group. But the degree of finger edema did not significantly differ between the two groups (F=1.46, p=.240). Conclusion: Passive exercise for upper extremities should be encouraged for elderly patients with chronic stroke to enhance the upper extremities' functional capacity as well as depression.

Effects of Lumbar Mobilization on the Paravertebral Muscle Activity and Muscle Tone in Patients with Lumbar Spinal Stenosis

  • Go, Junhyeok;An, Hojung
    • Journal of International Academy of Physical Therapy Research
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    • v.12 no.1
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    • pp.2302-2307
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    • 2021
  • Background: Patients with lumbar spinal stenosis show abnormal changes in muscle activity due to pain and limited range of motion of the lumbar spine. Excessive increased muscle tone and decreased muscle activity patterns threaten the patients' quality of life. However, there have been a few studies showing how to improve muscle performance in patients with lumbar spinal stenosis. Among these, joint mobilization is one way of improving muscle performance through pain relief and increasing the range of motion. Objectives: To investigate the effect of lumbar mobilization by orthopedic manual physical therapy on paravertebral muscle activity and tone in patients with lumbar spinal stenosis. Design: A randomized controlled trial. Methods: In this study, 24 patients with lumbar spinal stenosis were randomized (1:1 ratio) into two groups. The experimental group underwent lumbar posteroanterior mobilization, and the control group underwent conventional physical therapy (conventional transcutaneous electrical nerve stimulation) for 15 minutes each. For outcome measures, Myoton®PRO was used to evaluate muscle tone when resting of the paravertebral muscle in the pain area. For muscle activity evaluation, the reference voluntary contraction of the paravertebral muscle was evaluated using surface electromyography. Results: Muscle tone and activity were significantly improved after intervention in both the experimental and control groups. In addition, the experimental group showed more significant decrease in muscle tone and activity than the control group. Conclusion: These results suggest that lumbar mobilization improving muscle performance in patients with lumbar spinal stenosis.

INFLUENCE OF IMPLANT FIXTURE DESIGN ON IMPLANT PRIMARY STABILITY (임플랜트 고정체의 형태가 임플랜트 초기안정성에 미치는 영향)

  • Oh, Gap-Yong;Park, Sung-Hwa;Kim, Seok-Gyu
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.1
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    • pp.98-106
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    • 2007
  • Statement of problem: Current tendencies of the implant macrodesign are tapered shapes for improved primary stability, but there are lack of studies regarding the relationship between the implant macrodesign and primary stability. Purpose: The purpose is to investigate the effect of implant macrodesign on the implant primary stability by way of resonance frequency analysis in the bovine rib bones with different kinds of quality. Material and method: Fifty implants of 6 different kinds from two Korean implant systems were used for the test. Bovine rib bones were cut into one hundred pieces with the length of 5 cm. Among them forty pieces of rib bones with similar qualities were again selected. For the experimental group 1, the thickness of cortical part was measured and 20 pieces of rib bones with the mean thickness of 1.0mm were selected for implant placement. For the experimental group 2, the cortical parts of the remaining 20 pieces of rib bones were totally removed and then implants were placed on the pure cancellous bone according to the surgical manual. After placement of all implants, the implant stability quotient(ISQ) was measured by three times, and its statistical analysis was done. Results: There are statistically significant differences in ISQ values among 4 different kinds of Avana system implants in the experimental group 2. For the experimental group 1, Avana system implants showed significantly different ISQ values, but when differences in the thickness of cortical parts were statistically considered, did not show any significant differences in ISQ values. Among Oneplant system implants, there are no significant differences in ISQ values for the experimental group 2 as well as for the experimental group 1. Conclusion: Within the limits of this study, bone quality and implant design have some influences on the primary stability of implants. Especially in the bone of poor quality, tapered shape of implants are more favorable for the primary stability of implants.

A Study of Utilizing 2D Photo Scan Technology to Efficiently Design 3D Models (2D 포토 스캔 기술을 활용한 효율적인 3D 모델링 제작방법 연구)

  • Guo, Dawei;Chung, Jeanhun
    • Journal of Digital Convergence
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    • v.15 no.7
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    • pp.393-400
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    • 2017
  • Generally, in special effect video and 3D animation design process, character and background's 3D model is built by 3D program like MAYA or 3DS MAX. But in that manual modeling mode, model design needs much time and costs much money. In this paper, two experimental groups are set to prove use 2D photo scan modeling mode to build 3D model is effective and advanced. The first experimental group is modeling the same object by different experimental setting. The second experimental group is modeling the same background by different experimental setting. Through those two experimental groups, we try to find an effective design method and matters need attention when we use photo scan design mode. We aim to get the model from whole experiment and prove photo scan modeling mode is effective and advanced.

Neuromuscular electrical stimulation improves strength, pain and weight distribution on patients with knee instability post surgery

  • Asakawa, Yasuyoshi;Jung, Ji-Hye;Koh, Si-Eun
    • Physical Therapy Rehabilitation Science
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    • v.3 no.2
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    • pp.112-118
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    • 2014
  • Objective: The purpose of this study was to investigate the effects of an exercise with and without neuromuscular electrical stimulation (NMES) of the quadriceps femoris muscle, on strength, pain, and weight distribution in patients with knee instability post surgery. Design: Randomized controlled trial. Methods: Twenty patients in the early stage of rehabilitation after knee surgery were recruited as subjects and were randomly divided into either experimental group (exercise combined with NMES) (n=10) or control group (n=10). Both groups received strength training of the lower limb for 20 min/day, 5 days/week for 4 weeks. The experimental group used NMES for unilateral quadriceps femoris training with incremental increases in the intensity of isometric contraction over 4 weeks. Outcome measurements were assessed using the digital manual muscle testing, 30-chair stand test (30CST), numeric pain rating scale (NPRS) and weight distribution using the foot analyzer before and after 4 weeks of training. Results: After the 4-week intervention, knee extensor strength increased significantly in the experimental group post intervention (p<0.05), and there was a significant improvement in the experimental group compared with the control group (p<0.05). The 30CST and NPRS scores improved significantly in the experimental group compared to the control group (p<0.05), and there was a significant difference between the two groups (p<0.05). Weight distribution was significantly improved in the experimental group compared with the control group, (p<0.05), but there was no significant difference in improvement between the two groups. Conclusions: This study showed that NMES combined with strengthening exercises of the lower limbs is effective in improving lower limb pain and strength in patients with instability after knee surgery.