• 제목/요약/키워드: experiment and comparison

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Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-${\kappa}B$ in Acute Transection Spinal Cord Injury in Rats

  • Shrestha, Rajiv Prasad;Qiao, Jian Min;Shen, Fu Guo;Bista, Krishna Bahadur;Zhao, Zhong Nan;Yang, Jianhua
    • Journal of Korean Neurosurgical Society
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    • 제56권5호
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    • pp.375-382
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    • 2014
  • Objective : To assess the effect of bone marrow mononuclear cells (BMMNCs) transplantation in the expression of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) in spinal cord injury (SCI) in rats. Methods : BMMNCs were isolated from tibia and femur by a density gradient centrifugation. After establishment of acute transection SCI, rats were divided into experiment (BMMNCs), experiment control (0.1 M PBS infused) and sham surgery groups (laminectomy without any SCI). Locomotor function was assessed weekly for 5 weeks post-injury using BBB locomotor score and urinary bladder function daily for 4 weeks post-injury. Activity of NF-${\kappa}B$ in spinal cord was assessed by immunohistochemistry and reverse transcriptase polymerase chain reaction. Results : At each time point post-injury, sham surgery group had significantly higher Basso, Beattie, Bresnahan locomotor and urinary bladder function scores than experiment and experiment control group (p<0.05). At subsequent time interval there were gradual improvement in both experiment and experiment control group, but experiment group had higher score in comparison to experiment control group (p<0.05). Comparisons were also made for expression of activated NF-${\kappa}B$ positive cells and level of NF-${\kappa}B$ messenger RNA in spinal cord at various time points between the groups. Activated NF-${\kappa}B$ immunoreactivity and level of NF-${\kappa}B$ mRNA expression were significantly higher in control group in comparison to experiment and sham surgery group (p<0.05). Conclusion : BMMNCs transplantation attenuates the expression of NF-${\kappa}B$ in injured spinal cord tissue and thus helps in recovery of neurological function in rat models with SCI.

Comparison with Some Antioxidants on Hydroxyl Radical in Mouse Whole Brain Culture

  • Lee, Jeong-Chae;Lim, Kye-Taek;Lee, Ki-Seoup;Jung, Hee-young
    • Toxicological Research
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    • 제14권4호
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    • pp.541-545
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    • 1998
  • This experiment carried out to compare the protective effects of some antioxidants to hydroxyl radicals in embryonic mouse whole brain tissue culture. The ICR mouse whole brain (13 embryonic day) was cultured in hydroxyl radical system in which radicals were generated by 20 mU / ml glucose oxidase (GO). In this experiment, to make ferrous iron from ferric iron, iron as an accelerator, and ascorbic acid as a reductant were used. For comparison of the protective effects to hydroxyl radicals, antioxidants such as desferrioxamine (DFX), laccase. water or ethanol extracts from Rhus Vemiciflua Stokes (RVS), and $\alpha$-tocopherol were used, because they relate to metal ion. The results of this experiment showed that all antioxidants protected effectively the cytotoxicity from hydroxyl radicals in the brain cultures. More than 70% of cell viabilities among different antioxidants was at 1 mM DFX, 1.43 $\mu\textrm{m}$ laccase, 12.5 $\mu\textrm{m}$ water extract, 12.5 $\mu\textrm{m}$ ethanol extract and 50 $\mu\textrm{m}$ $\alpha$-tocopherol individually, compared with 20 mU/ml GO alone. In comparison to the antioxidative activities of antioxidants, laccase and extracts from RVS showed strong antioxidative effects even at low concentration.

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2엽형 수직축 풍력발전기의 유동해석 및 실험 비교 (AERODYNAMIC ANALYSIS AND COMPARISON OF EXPERIMENTAL DATA FOR 2-BLADED VERTICAL AXIS WIND TURBINE)

  • 황미현;김동현;이종욱;오민우;김명환;류경중
    • 한국전산유체공학회지
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    • 제15권4호
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    • pp.85-91
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    • 2010
  • In this study, aerodynamic analyses based on unsteady computational fluid dynamics (CFD) have been conducted for a 2-bladed vertical-axis wind turbine (VAWT) configuration. Reynolds-averaged Navier-Stokes equations with standard $k-{\varepsilon}$ and SST $k-{\varepsilon}$ turbulence models are solved for unsteady flow problems. The experiment model of 2-bladed VAWT has been designed and tested in this study. Aerodynamic experiment of the present VAWT model are effectively conducted using the vehicle mounted testing system. The comparison result between the experiment and the computational fluid dynamics (CFD) analysis are presented in order to verify the accuracy of CFD modeling with different turbulent models.

5kVA 3레벨 PFC 컨버터의 열해석 모의 및 실험적 비교 (5kVA 3-level PFC Converter in Thermal Analysis : Comparison of Simulation and Experiment)

  • 이도홍;한유;최동민;조영훈;최규하
    • 전력전자학회:학술대회논문집
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    • 전력전자학회 2017년도 전력전자학술대회
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    • pp.359-360
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    • 2017
  • This paper presents comparison of simulation and experiment in thermal analysis of 3-level PFC. Thermal analysis is necessary to optimize the power conversion system to get higher efficiency. Accordingly, analysis via simulation tools and analytic methods is performed to predict the power losses of converter. Consequently, thermal results of 3-level PFC experiment is compared with thermal analysis.

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헤드폰을 이용한 가청역치레벨의 측정 방법 비교 (Comparison of a hearing threshold level using a headphone)

  • 김득성;장서일;김동준;이연수
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2007년도 추계학술대회논문집
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    • pp.796-801
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    • 2007
  • This research presents a laboratory study about a comparison between two methods that measure a hearing threshold of the Subjects who participated in an experiment of the Jury test. The Subjects heard a signal by a headphone. The Subjects of total 63 persons (man=42, woman=21 persons) participated in an experiment. A test of hearing was divided into two (Top-Down, Bottom-Up) methods. Total time of hearing test was about 80 minute(40min/day). As a results of the hearing test, a hearing threshold of the Subjects who used to wear a headphone was higher than that of the Subjects who not used to wear a headphone. A hearing threshold of a man was higher than that of a woman. The result of hearing test was showed that ISO's hearing threshold(MAF) was lower than a result that get from an experiment.

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Comparison of Effects of Non-surgical Continuous and Intermittent Traction on Pain, Balance and Physical Function in the Treatment of Knee Osteoarthritis

  • Lee, Dong-Kyu;Jeong, Jin-Gyu
    • The Journal of Korean Physical Therapy
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    • 제32권6호
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    • pp.348-353
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    • 2020
  • Purpose: This study examined the comparison of effects of non-surgical continuous and intermittent traction on pain, balance and physical function in the treatment of knee osteoarthritis. Methods: A total of 30 knee osteoarthritis patients were recruited and randomized to a continuous traction group (n=10), an intermittent traction group (n=10), and a control group (n=10). The continuous traction group and intermittent traction group received a non-surgical continuous and intermittent knee joint traction workout five times a week, for 4 weeks. All subjects were assessed with the numeric rating scale (NRS), timed up and go test (TUGT), and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) before and after the 4-week treatment. Results: As a result of comparison within groups, the continuous traction group, intermittent traction group, and control group showed a significant difference for NRS, TUGT, and WOMAC after the experiment (p<0.05). According to the comparison of the three groups, the continuous traction group showed a more effectively significant difference than the intermittent traction group and the control group in the balance and physical functions before and after the experiment (p<0.05). Conclusion: This study showed that non-surgical continuous traction treatment was effective in improving pain, balance ability, and physical function in knee osteoarthritis patients.

升麻葛根湯加味方이 제I형 알레르기를 실험적으로 유발한 흰쥐의 免疫細胞및 血淸IgE에 미치는 영향 (The Effects of sumgmagalguntanggamibang(SMG) on the Immunocyte and Serum IgE in the Murine of type I Hypersensitivity Induced by the Experiment)

  • 강기홍;김윤범;채병윤
    • 한방안이비인후피부과학회지
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    • 제14권1호
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    • pp.129-153
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    • 2001
  • Background: SMG (升麻葛根湯加味方) is an herbal medicine which has been used in oriental medicine as a traditional therapeutic agent of pruritus and skin disease. Objective: This study was performed to investigate the effect of SMG on the anti-hypersensitivity and immune response in the murine of type I hypersensitivity induced by the experiment. Materials and Methods: Laboratory rats were primary sensitized with OA (ovalbumin); on day 1, rats of a Control group and Sample group (SMG group) were systemically immunized by subcutaneous injection of 1mg OA and 300mg of Al(OH)3 in a total volume of 2ml saline. The rats of the sample group were orally administered with an SMG water extract for 14 days after primary immunization. On day 14 after the systemic immunization, rats received local immunization by inhaling $0.9\%$ saline aerosol containing $2\%$(wt/vol) OA. A day after local immunization, BAL fluid and peripheral blood were collected from the rats. Total cell, lymphocyte, $CD4^+\;T\;cell,\;CD8^+\;T\;cell,\;CD4^+/CD8^+$ ratio in the BALF, and IgE, $CD4^+\;T\;cell,\;CD8^+$ T cell in the peripheral blood were measured and evaluated. Results: SMG showed a suppressive effect on the immune response in the rats. 1. Total Cells in the BALF decreased in the SMG treated group in comparison group, but statistic differences were not observed. 2. Total lymphocytes in the BALF were statistically decreased in SMG treated group in comparison to the control group. 3. CD4+ T cells in the BALF were statistically decreased in SMG treated group in comparison to the control group. 4. CD8+ T cells in the BALF were decreased in SMG treated group in comparison to the control group, but statistic differences were not observed. 5. The ratio of CD4+/CD8+ in the BALF was statistically decreased in SMG treated group in comparison to the control group. 6. The IgE level in serum was statistically decreased in SMG treated group in comparison to the control group. 7. The ratio of CD4+ and CD8+ in peripheral blood showed undetectable differences between each group of rats. From the experiment cited above, this study shows that SMG has both anti-hypersensitivity effects and immunoregulatory effects when administered to rats. Based on this experiment, it is suggested that SMG could be a useful immunomodulator and anti-allergy agent.

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재배적 안정성이 높은 참깨 계통 선발 (Selection of Varieties with Higher Cultural Stability in Sesamum indicum)

  • 심강보;강철환;이성우;김동휘;채영암
    • 한국작물학회지
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    • 제45권6호
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    • pp.374-381
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    • 2000
  • This study was conducted to select sesame varieties with high cultural stabilities by comparing several parameters of agronomic traits under the different cultural environments. Of the six areas, Iksan and Jinju areas which showed positively larger environment index values were relatively adequate cultural conditions for sesame. At the comparison of cultural stability of agronomic traits by Eberhart and Russell regression model among sesame breeding lines, Suwon 169 showed more stable regression coefficient values to the number of capsules per plant, number of seeds per capsule and seed weight per plant, and Iksan 12 showed more stable regression coefficient values to culm length and weight per plant. At the comparison of cultural stability of yield per 10a, Suwon 169 and Iksan 12 among sesame breeding lines showed more stable respectively, deviation values of 0.99, 0.98 respectively, and more less regression deviation values of 0.074, 0.167 respectively. Therefore those breeding lines are comparatively higher stabilities to yield determining agronomic traits under the different cultural environments, and it was concluded that those two breeding lines had the possibility to recommend promising breeding lines in the future.

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수중과 지상에서 대각선 패턴 운동이 만성 뇌졸중 환자의 균형 자신감과 호흡 기능에 미치는 효과 비교 (Comparing the Effects of Underwater and Ground-Based Diagonal Pattern Exercises on the Balance Confidence and Respiratory Functions of Chronic Stroke Patients)

  • 박재철;이동규
    • PNF and Movement
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    • 제19권2호
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    • pp.173-182
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    • 2021
  • Purpose: The purpose of this study was to compare the effects of underwater and ground-based diagonal pattern exercises on the balance confidence and respiratory functions of chronic stroke patients. Methods: Thirty chronic stroke patients were assigned randomly to an experimental (n = 15) or control (n = 15) group. The experimental group performed an underwater diagonal pattern exercise. The control group performed a ground-based diagonal pattern exercise. Training was conducted once a day for 30 minutes, five days per week for six weeks. Balance confidence was measured using the Activities-Specific Balance Confidence Scale-Korea version. Respiratory function was measured using a spirometer. Results: In a comparison within groups, the experimental and control groups showed significant differences in balance confidence after the experiment (p < 0.05). In a comparison between the two groups, the experimental group showed a more significant difference in balance confidence than the control group (p < 0.05). In a comparison within groups, the experimental group showed a significant difference in respiratory functions after the experiment (p < 0.05). In a comparison between the two groups, the experimental group showed a more significant difference in respiratory functions than the control group (p < 0.05). Conclusion: Based on these results, underwater diagonal pattern exercises effectively improved the balance confidence and respiratory functions of chronic stroke patients.