Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-${\kappa}B$ in Acute Transection Spinal Cord Injury in Rats

  • Shrestha, Rajiv Prasad (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) ;
  • Qiao, Jian Min (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) ;
  • Shen, Fu Guo (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) ;
  • Bista, Krishna Bahadur (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) ;
  • Zhao, Zhong Nan (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University) ;
  • Yang, Jianhua (Department of Orthopaedics, First Affiliated Hospital of Jiamusi University)
  • Received : 2013.12.19
  • Accepted : 2014.08.16
  • Published : 2014.11.28
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Abstract

Objective : To assess the effect of bone marrow mononuclear cells (BMMNCs) transplantation in the expression of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) in spinal cord injury (SCI) in rats. Methods : BMMNCs were isolated from tibia and femur by a density gradient centrifugation. After establishment of acute transection SCI, rats were divided into experiment (BMMNCs), experiment control (0.1 M PBS infused) and sham surgery groups (laminectomy without any SCI). Locomotor function was assessed weekly for 5 weeks post-injury using BBB locomotor score and urinary bladder function daily for 4 weeks post-injury. Activity of NF-${\kappa}B$ in spinal cord was assessed by immunohistochemistry and reverse transcriptase polymerase chain reaction. Results : At each time point post-injury, sham surgery group had significantly higher Basso, Beattie, Bresnahan locomotor and urinary bladder function scores than experiment and experiment control group (p<0.05). At subsequent time interval there were gradual improvement in both experiment and experiment control group, but experiment group had higher score in comparison to experiment control group (p<0.05). Comparisons were also made for expression of activated NF-${\kappa}B$ positive cells and level of NF-${\kappa}B$ messenger RNA in spinal cord at various time points between the groups. Activated NF-${\kappa}B$ immunoreactivity and level of NF-${\kappa}B$ mRNA expression were significantly higher in control group in comparison to experiment and sham surgery group (p<0.05). Conclusion : BMMNCs transplantation attenuates the expression of NF-${\kappa}B$ in injured spinal cord tissue and thus helps in recovery of neurological function in rat models with SCI.

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References

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