• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.776-780
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• 2001

Based on the static and dynamic adsorption of Streamline DEAE, a modified tank-in-series model including particle size distribution was used to describe the adsorption performance of bovine serum albumin in an expanded bed. The calculated results indicated that the suggested model was able to simulate breakthrough curves under various conditions.

• KSBB Journal
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• v.16 no.2
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• pp.146-152
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• 2001

To avoid the intrinsic problem of aggregation associated with the traditional solution-phase refolding process, we propose a solid-phase refolding method integrated with expanded bed adsorption chromatography. The model protein used was a fusion protein of recombinant human growth hormone and a glutathione S transferase fragment. It was demonstrated that the EBA-mediated refolding technique could simultaneously remove cellular debris and directly renature the fusion protein inclusion bodies in the cell homogenate with much higher yields and less agregation. To demonstrate the applicability of the method, we successfully tested the three representative types of starting materials, i. e., rhGH monomer, washed inclusion bodies, and the E. coli homogenate. This direct and simplified refolding process could also reduce the number of renaturation steps required and allow refolding at a higher concentration, at approximately 2 mg fusion protein per ml of resin. To the best of our knowledge, it is the first approach that has combined the solid-phase refolding method with expanded bed chromatography.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.3
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• pp.280-283
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• 2005

The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography and Streamline DEAE $(\rho=1.2g/cm^3)$ from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored by employing yeast cells in a concentration ranged of 0 to $15\%(w/v)$. Equilibrium isotherms for adsorption of BSA on Streamline DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins to bind to the sites on adsorbents.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.6
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• pp.552-555
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• 2005

The influence of whole yeast cells $(0{\sim}15%\;w/v)$ on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent $(p=1.5\;g/cm^3)$ derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dynamic binding capacity of BSA at a $C/C_0=0.1$ biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5mg/mL of settled adsorbent, respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.4
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• pp.323-325
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• 2004

To model the behavior of expanded beds with aqueous feed streams containing different amounts of glycerol, we previously developed a modified Stokes expression that correlates the terminal settling velocity of a particle of a solution with the properties of the particle (particle diameter and density) and the solution (density and viscosity). Two empirical parameters, the effective diameter of the poly-disperse resins for protein adsorption and an exponent of non-unity for $(\rho_{P}-\rho)/\mu$ term, are introduced in the modified Stokes expression. We applied the same type of the modified Stokes expression in combination with the Richardson-Zaki correlation to the published results [1], and found that the expansions of the beds with feed streams containing different amounts of E. coli homogenates can also be successfully described.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2006.11a
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• pp.97-102
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• 2006

The purpose of this study is to suggest complementary measures to the statute legalizing the expansion of living space to balcony, by means of survey on the recognition and preference of preliminary applicants for new apartments. The survey was done on 130 preliminary applicants who visited 3 model houses opened in Cheongju in June to July, 2006. Conclusions are as follows. (1) Most of preliminary applicants knew that the expansion of living space to balcony were legalized, but were not quite aware of legal details, so that more active publicity is needed by the government. In addition, if the government has a plan to impose tax to expanded space, it has to make clear the definition of the balcony space. (2) Preliminary applicants wanted space expansion of living room, main bed room and children's bed room in order, which implies that constructors have to suggest variety of expansion plans overpassing simple space expansion. (3) Preliminary applicants attached importance on the safety of expanded space. The management office is recommended to have the right to superintend its safety after construction.

• International conference on construction engineering and project management
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• 2015.10a
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• pp.665-666
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• 2015

Recently BIM technology has been expanded for using in construction project. However its spread has been delayed than the initial expectations, due to the high-cost of BIM infrastructure development, the lack of regulations, the lack of process and so forth. In construction site phase, especially the analysis of current research trend about IT technologies, virtualization and BIM service, data exchange such as drawing, 3D model, object data, properties using cloud computing and virtual server system is defined as a most successful solution. The purpose of this study is enable the cloud computing BIM server to provide several main function such as edit a model, 3D model viewer and checker, mark-up, snapshot in high-performance quality by proper design of VDI system. Concurrent client connection performance is a main technical index of VDI. Through test-bed server client, developed VDI system's multi-connect control will be evaluated. The performance-test result of BIM server VDI will effect to development direction of cloud computing BIM service for commercialization.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.10 no.6
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• pp.140-145
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• 2011

An anisotropic beam model is proposed by employing an asymptotic expansion method for thermo-mechanical multiphysics environment. An asymptotic method based on virtual work is introduced first, and then the variables of mechanical displacement and temperature rise are asymptotically expanded by taking advantage of geometrical slenderness of elastic bodies. Subsequently substituting these expansions into the virtual work principle allows us to asymptotically expand the virtual work. This will yield a set of recursive virtual works from which two-dimensional microscopic and one-dimensional macroscopic equations are systematically derived at each order. In this way, homogenized stiffnesses and thermomechanical coupling coefficients are derived. To demonstrate the validity and efficiency of the proposed approach, composite beams are taken as a test-bed example. The results obtained herein are compared to those of three-dimensional finite element analysis.

• Journal of the Korean Society of Hazard Mitigation
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• v.7 no.5
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• pp.151-158
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• 2007

After installing calibrated depth scale at 11 different points inside the nine streams network for estimating time of concentration of stream in Young-Heung island near coastal region of Incheon we estimated the flow against actual rainfall events. By considering time of concentration formula which here mainly used for estimating the time of concentration from practical experiments, compared were three methods of inflow time and accepted to method of used the chart. The maximum flow occurrence time was estimated by an outflow model and observed maximum flow occurrence time were determined by to a comparative analysis. Kirpich formula was selected as the proper formula for calculating the concentration time inside the island streams. Kirpich formula could be applicable for the expanded range while catchment area of $0.453km^2$ and channel bed slope of $3{\sim}5%$ to catchment area of $2.0km^2$ and channel bed slope of about 1.5%.

• Journal of Korea Water Resources Association
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• v.55 no.1
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• pp.43-57
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• 2022

Advances in measurement techniques have reduced measurement costs and enhanced safety resulting in less uncertainty. For example, an acoustic doppler current profiler (ADCP) based suspended sediment concentration (SSC) measurement technique is being accepted as an alternative to the conventional data collection method. In Korean rivers, horizontal ADCPs (H-ADCPs) are mounted on the automatic discharge monitoring stations, where SSC can be measured using the backscatter of ADCPs. However, automatic discharge monitoring stations and sediment monitoring stations do not always coincide which hinders the application of the new techniques that are not feasible to some stations. This work presents and analyzes H-ADCP-SSC models for 9 discharge monitoring stations in Korean rivers. In application of the Gaussian mixture model (GMM) to sediment-related variables (catchment area, particle size distributions of suspended sediment and bed material, water discharge-sediment discharge curves) from 44 sediment monitoring stations, it is revealed that those characteristics can distinguish sediment monitoring stations regionally. Linking the two results, we propose a protocol determining the H-ADCP-SSC model where no H-ADCP-SSC model is available.