• Ocean and Polar Research
• /
• v.32 no.1
• /
• pp.63-71
• /
• 2010

An experiment was conducted to investigate the effects of temperature and stocking density on daily patterns and rates of total ammonia nitrogen (TAN) excretion in juvenile red seabream Pagrus major (mean body weight: 29.0 g) under fasting and feeding conditions. Fish were acclimated over 7 days under four different temperatures (10, 15, 20, and $25^{\circ}C$) and at two different densities (5.5 and $11.0\;kg\;m^{-3}$). Each treatment had three replicates and a total of 216 fish were used. After 72 hours starvation, endogenous TAN excretion was measured for each temperature and density. To investigate exogenous TAN excretion, fish were handfed a commercial diet containing 51.6% crude protein twice a day for 7 days, at 08:00 and 16:00. Water was sampled from both inlets and outlets of chambers every 2 hours over a 24 hour period. Both endogenous and exogenous TAN excretion increased with increases in temperature and density (P<0.05). Mean daily endogenous TAN excretion rates at 10, 15, 20, and $25^{\circ}C$ were 88.8, 101.1, 125.0, and $143.3\;mg\;TAN\;kg^{-1}\;d^{-1}$ at low density, and 105.2, 119.2, 141.5, and $168.8\;mg\;TAN\;kg^{-1}\;d^{-1}$ at high density, respectively. Mean daily exogenous TAN excretion rates at 10, 15, 20, and $25^{\circ}C$ were 343.5, 403.7, 535.7, and $601.7\;mg\;TAN\;kg^{-1}\;d^{-1}$ at low density, and 391.9, 479.7, 611.9, and $683.4\;mg\;TAN\;kg^{-1}\;d^{-1}$ at high density, respectively. The exogenous TAN excretion rate peaked 10~12 hours after the first feeding under all temperatures and densities. The TAN loss for ingested nitrogen increased with increases in temperature and density (P<0.05), ranging from 27.9 to 50.1% at low density and 31.7 to 56.9% at high density. This study provides empirical data for estimating ammonia excretion and managing the culture of red seabream under the given temperatures and densities.

• Journal of Animal Environmental Science
• /
• v.17 no.sup
• /
• pp.35-42
• /
• 2011

This work was conducted to investigate the effect of dietary phytase on growth performance and excreta excretion of growing-finishing pigs for 7 days. Eighty three crossbreeds (Yorkshire ${\times}$ Landrace ${\times}$ Duroc) of growing-finishing pigs were used in this work, and divided into 6 treatments. Six treatments were compared in a $2{\times}2$ factorial arrangement with 2 groups (5 replications/group, 8 head/replications) with the additive phytase and 3 groups(growing I, II and finishing phase) with growing phases. Initial weights with growing phases were $58.6{\pm}3.9$. $83.2{\pm}3.8$ and $111.4{\pm}5.4kg$, respectively. Body weight gain was high in phytase treatment (P<0.01) and low at finishing phase. Feed conversion ratio was high in no phytase treatment and at finishing phase (P<0.05). Feed and water intakes have no significant difference with phytase existence and growing phases. Feces excretion decreased with growing phase (P<0.05), and was low at phytase treatment (P<0.05). There was no significant difference on urine excretion (P>0.05). Nitrogen (N) and phosphorus (P) intake was not found significant difference with phytase and growing phases. N excretion had no significant difference on phytase existence and growing phase (P>0.05), but P excretion decreased at phytase treatment (P<0.05). N and P excretion ratio was low at phytase treatment (P<0.05). Finally, dietary phytase resulted in improvement of growth performance and reduction of excreta excretion of growing-finishing pigs.

• Korean Journal of Community Nutrition
• /
• v.20 no.6
• /
• pp.460-467
• /
• 2015

Objectives: Excess sodium intake has been linked to obesity and obesity-related indices. However, the scientific evidence for this association is inadequate. The purpose of this study was to investigate the association between urinary sodium excretion and obesity-related indices among Korean adults. Methods: A convenience sample of 120 subjects (60 obese and 60 non-obese subjects) were recruited applying frequency matching for sex and age between two groups. Sodium intake level was assessed through 24-hour urine collection. Obesity-related metabolic risk factors, including fasting blood lipid indices, subcutaneous and visceral fat through computed tomography (CT), insulin resistance indices, blood pressure and liver enzymes were measured in all subjects. These obesity-related metabolic risk factors were compared between obese and non-obese group according to sodium excretion levels (<110 mEq/day, 110~180 mEq/day, >180 mEq/day). Results: After adjusting for age, gender, health behaviors (smoking, exercise, drinking), and energy intake, several obesity-related metabolic risk factors, including abdominal circumference, body fat percentage, subcutaneous and visceral fat, triglyceride, and systolic blood pressure were found to be significantly deteriorated as the sodium excretion level increases. In addition, multivariate adjusted-odds ratios of abdominal obesity, high blood triglyceride, and high blood pressure were found significantly higher in the highest sodium excretion group compared to the lowest group. The mean number of metabolic syndrome risk factors was also significantly greater in the highest sodium excretion group than in the lowest group. Conclusions: The current study findings suggested that high sodium intake can affect obesity and metabolic syndrome risk negatively, implying the necessity of future research on low-sodium diet intervention in relation to obesity and related health problems.

• Journal of Nutrition and Health
• /
• v.20 no.4
• /
• pp.246-257
• /
• 1987

The present study was conducted to examine the effect of an increased level of dietary sodium on calcium excretion in 8 health young adult Korean women on a controlled diet. After adaptation period of 2 days, each subject received 2811.8$\pm$68.1 mgNa(day during the initial period of 5 days (low sodium period) and 6417.1$\pm$248.6mgNa(day during the following period of 5 days (high sodium period). Calcium intake was 593.7$\pm$15.7 mg Ca/day during the low sodium period of 596.1$\pm$25.1 Ca/day duing the high sodium period. When the low sodium period is compared with the high soidum period, the results were as following. 1) Mean urinary sodium excretion was significantly higher during the high sodium period (5760.1$\pm$156.5mg0 than during the low sodium period (2272.2$\pm$108.6mg)(P<0.001). Fecal sodium excretion of the high sodium period was also significantly higher than that of the low sodium period(P<0.001). Mean value of sodium balance during the high sodium period was higher than that of the low sodium period . However, the difference was not significant. 2) Mean urinary calcium excretion was significantly higher during the high sodium period than during the low sodium period ; mean value of the low sodium period was 124.7$\pm$11.3mg and that of the high sodium period was 202.6$\pm$17.2mg)P<0.001). Fecal calcium excretion was higher during the high sodium period (284.9$\pm$31.0mg) than during the low sodium period (253.9$\pm$15.3mg0, but there was no significance. Mean value of calcium balance during the high sodium period was significantly lower than that of the low sodium period(P<0.001). The above results show that high sodium intake increases calcium excretion as well as sodium excretion.

• Journal of Nutrition and Health
• /
• v.36 no.5
• /
• pp.476-482
• /
• 2003

We studied the effects of soy isoflavone supplements on bone metabolism marker (serum osteocalcin, urinary deoxypyridinoline) and urinary mineral excretion (urinary Ca, Mg, Zn) in 47 postmenopausal women. There were 24 participants in the treatment group and 23 in the control group. The treatment group consumed isoflavone extract capsules daily (which contained 90 mg of soy isoflavones) for 12 weeks. The study compared before and after isoflavone intake in the following areas: Physical examination, diet survey, bone metabolism marker and urinary mineral excretion. The average age of the treatment group was 64.6 years and that of the control group was 66.5 years. There were no significant differences between the two groups in terms of height, weight and body mass index. Both groups maintained a regular diet pattern in terms of their average daily nutrient intake. There were no significant differences between the treatment group (23.9 mg) and the control group (25.4 mg) in terms of daily isoflavone intake based on diet. The analysis of bone metabolism marker changes in the treatment group after 12 weeks of taking the isoflavone supplements demonstrated significant differences in the following: Serum osteocalcin (13.7 ng/mL in befor versus 6.8 ng/mL in after) and urinary deoxypyridinoline (5.9 nmol/mmol Cr in befor versus 4.5 nmol/mmol Cr in after). The subjects in the treatment group showed no significant difference in urinary Ca excretion. But the subjects showed a significant difference in urinary Mg (131.9 mg/day in befor versus 115.6 mg/day in after) and Zn (400.5 $\mu\textrm{g}$/day in befor versus 310.2 $\mu\textrm{g}$/day in after) excretion in the isoflavone treatment group at the levels of p<0.001, p<0.01, respectively. No changes were made in the intake of minerals. The composition of serum osteocalcin and urinary deoxypyridinoline, and indicators of bone metabolism, including the excretion Mg and Zn, significantly decreased. As a result, bone mineral loss was lessened. (Korean J Nutrition 36(5): 476~482, 2003)

• Journal of Nutrition and Health
• /
• v.19 no.1
• /
• pp.32-40
• /
• 1986

The present study conducted to examine the effects of proteins from different sources on Ca excretion in 6 healthy young adult Korean women. The subjects were given meat protein diet for 5 days and soy protein diet for subsequent 5 days. the two diets were similar in protein and Ca contents. Urinary and fecal samples were collected for the last 2 days of each diet period and were analyzed for Ca and P. The results were as following ; 1) Mean daily urinary calcium excretion was 126.5$\pm$22.70mg for meat protein diet and 83.7$\pm$17.19mg for soy protein diet and the difference was significant (P<0.025). 2) Fecal Ca excretion did not show significant difference between two experimental preiod ; 466.9$\pm$73.68 mg of meat portein diet 284.4$\pm$54.96mg for soy protein diet. 3) Three out of six subjects showed negative balance on meat protein diet, but only one showed negative balance on soy protein diet. The averageof the balances on meat protein diet was -65.4 $\pm$68.19 and that of soy protein diet was 155.3$\pm$52.28 ; the difference was significant(P<0.025). 4) Urinary P excretion tended to be higher on meat protein diet but was not significant compared to soy protein diet . Fecal P excretion was significantly higher (P<0.001) on soy protein diet. Overall P balances for meat protein diet and soy protein diet were 219.94 mg and 229.46mg respectively (P<0.05). The above results show that urinary Ca excretion was significantly higher on meat protein diet but fecal excretion did not show significant difference between meat protein diet and soy protein diet. The overall Ca balance was significantly higher on Soy protein diet compared to meat protein diet.

• The Korean Journal of Nuclear Medicine Technology
• /
• v.13 no.1
• /
• pp.15-19
• /
• 2009

Purpose: The aim of study is to find a correlation between Salivary clearance rate using saliva and blood and Secretion rate and Excretion rate using Salivary gland Scan images. Materials and Methods: Salivary Scan and Stimulate clearance of $^{99m}Tc$-pertechnate was performed in 20 patients with moderate function(group 1), 9 patients with severe function glands (group 2), 3 patients with non function (group 3) and normal 6 controls. Salivay clearance rate was compare with Secretion rate and Excretion rate of Salivary glands' ROI. Result: Stimulate salivary clearance of normal controls was 18.4 ml/min, salivary clearance of group 1 was 10.1 ml/min, salivary clearance of group 2 was 10.4 ml/min and salivary clearance of group 3 was 2.3 ml/min. Significant difference was found between normal controls and group 2,3 (p<0.05, p<0.05). Secretion rate and Excretion rate of normal controls was 21.6%, 24.6%, Secretion rate and Excretion rate of group 1 was 17.6%, 24.0%, Secretion rate and Excretion rate of group 2 was 8.8%, 13.9% and Secretion rate and Excretion rate of group 3 was 5.6%, 2.9%. Significant difference was found between normal controls and group 2,3 (p<0.05, p<0.05). Conclusions: Stimulate salivary clearance using saliva and blood and Secretion rate and Excretion rate using Salivary gland Scan images accord well together.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.3
• /
• pp.386-391
• /
• 2017

Objective: Protein supplementation is costly and can result in excess nitrogen (N) excretion. The objective of this study was to evaluate the effects of feeding different levels of dietary protein on average daily gain, body size, rumen fermentation, and nitrogen excretion of 8 to 10 month-old Holstein heifers. Methods: Thirty-six Holstein heifers were divided into 12 blocks according to age ($273{\pm}6.2d$) and were randomly assigned to diets containing a low (10.2% dry matter [DM]), medium (11.9% DM), or high (13.5% DM) level of dietary crude protein (CP). All diets contained approximately 70% roughage and 30% concentrate with similar dietary metabolizable energy (ME) content (2.47 Mcal/kg). Results: Dry matter intake did not differ among the treatments, and average daily gain increased with the increasing dietary protein, 0.79, 0.95, 0.97 kg/d for low, medium, and high group, respectively. Body height increased linearly with increasing dietary CP but no other significant differences in body dimensions were found among the treatments. The increased ratios of dietary CP improved the rate of rear teat length growth remarkably (p<0.05). There was no difference in rumen pH or ruminal major volatile fatty acid (acetate, propionate, and butyrate) concentration among the 3 diets, but rumen ammonia-N concentration increased with the higher dietary CP (p<0.05). Increasing N intake led to increased total N excretion; urinary N excretion was significantly increased (p<0.05) but fecal N excretion was similar among the treatments. Conclusion: These data suggest that the diet containing 11.9% CP (ME 2.47 Mcal/kg) could meet the maintenance and growth requirements of 9 to 11 month-old Holstein heifers gaining approximately 0.9 kg/d.

• Journal of Nutrition and Health
• /
• v.39 no.1
• /
• pp.11-17
• /
• 2006

The effect of the level of casein phosphopeptide (CPP) on mineral (Ca and P) bioavailabilties and bone biomarker of aged ovariectomized (OVX) Sprague-Dawley rats were studied as a model for postmenopausal bone loss. Forty five Spargue dawley rats, 220-230 g of body weight were fed a control diet (AIN 93M) or containing different level of CPP diet for 7 weeks: $0\%$ (sham control; SC, OVX control; OC), $1\%$ (OVX low CPP diet: OL), $2\%$ (OVX medium CPP diet; OM), $3\%$ (OVX high CPP diet; OH) Ca absorption was unaffected by increasing CPP content from 0 to $3\%$. Urinary Ca excretion was increased by OVX, and decreased by CPP significantly (p < 0.05) with no evident doserelationship. The urinary P excretion was increased by CPP intake in OVX rats. The fecal excretion of P given CPP decreased in OVX with dose dependent manner. Ca and P contents of femur significantly increased by adding 2 or $3\%$ of CPP when compared with OC group and OL group (p < 0.05). There were no significant differences in serum alkaline phosphatase activity and c-terminal telopeptide excretion in experimental groups. Although ovariectomy induced the increase in urinary c-terminal telopeptide excretion, 2 or $3\%$ of CPP in the diet decreased urinary c-terminal telopetide excretion significantly. These finding suggest the usefulness of CPP in the prevention of postmenopausal bone loss by decreasing urinary Ca excretion and bone resorption. Over 2 percent of CPP in the diet was effective to prevent postmenopausal bone loss.

• Journal of Pharmaceutical Investigation
• /
• v.31 no.4
• /
• pp.209-216
• /
• 2001

The purpose of the present study was to investigate the hepatic uptake and biliary excretion of l-anilino-8-naphthalene sulfonate (ANS) in vivo. The plasma concentration and liver concentration of ANS were determined after its i.v. bolus administration at a dose of $30\;{\mu}mol/kg$ in rats. The hepatic uptake clearance $(CL_{uptake})$ of ANS was 0.1 ml/min/g liver. On the basis of the unbound concentration of ANS, the permeability-surface area product $(PS_{influx})$ was calculated to be l0.4 ml/min/g liver, being comparable of in vitro data. On the other hand, we determined the plasma concentration, liver concentration and biliary excretion rate of ANS at steady-state after its i. v. infusion $(0.2-1.6\;{\mu}mol/min/kg)$ in rats. The excretion clearance $(CL_{excretion})$ of ANS showed Michaelis-Menten kinetics with increasing the infusion rate. The permeability-surface area product $(PS_{excretion})$ based on the unbound concentration in the liver was calculated to be 0.0165 ml/min/g liver, which is negligible compared with the intrinsic clearance $(CL_{int}=3.3\;ml/min/g\;liver)$ by rat liver microsomes. The sequestration process of ANS, therefore, was considered to be mainly due to the metabolic process in the liver $(PS_{seq}{\risingdotseq}CL_{int})$. Furthermore, $PS_{efflux}$ value calculated from $PS_{influx}$ and $PS_{seq}$ was 4.4 ml/min/g liver, which was comparable of in vitro data. In conclusion, in vivo parameters such as $PS_{influx}$, $PS_{efflux}$ and $PS_{seq}$ in the present study showed good in vivo-in vitro relationship. Thus, the kinetic analysis method proposed in the present study would be useful to analyze the hepatic transport of drugs in vivo.