Browse > Article

Effect of Dietary Caseinphosphopeptide Levels on Bone Metabolism in Ovariectomized Rats  

Yang Daum (Department of Food and Nutrition, Kookmin University)
Lee Jin-Woo (Department of Dentistry, Dankook University)
Park Hong Joo (Rural Resources Development Institute)
Kim Sun Hee (Department of Food and Nutrition, Kookmin University)
Chang Moon-Jeong (Department of Food and Nutrition, Kookmin University)
Publication Information
Journal of Nutrition and Health / v.39, no.1, 2006 , pp. 11-17 More about this Journal
Abstract
The effect of the level of casein phosphopeptide (CPP) on mineral (Ca and P) bioavailabilties and bone biomarker of aged ovariectomized (OVX) Sprague-Dawley rats were studied as a model for postmenopausal bone loss. Forty five Spargue dawley rats, 220-230 g of body weight were fed a control diet (AIN 93M) or containing different level of CPP diet for 7 weeks: $0\%$ (sham control; SC, OVX control; OC), $1\%$ (OVX low CPP diet: OL), $2\%$ (OVX medium CPP diet; OM), $3\%$ (OVX high CPP diet; OH) Ca absorption was unaffected by increasing CPP content from 0 to $3\%$. Urinary Ca excretion was increased by OVX, and decreased by CPP significantly (p < 0.05) with no evident doserelationship. The urinary P excretion was increased by CPP intake in OVX rats. The fecal excretion of P given CPP decreased in OVX with dose dependent manner. Ca and P contents of femur significantly increased by adding 2 or $3\%$ of CPP when compared with OC group and OL group (p < 0.05). There were no significant differences in serum alkaline phosphatase activity and c-terminal telopeptide excretion in experimental groups. Although ovariectomy induced the increase in urinary c-terminal telopeptide excretion, 2 or $3\%$ of CPP in the diet decreased urinary c-terminal telopetide excretion significantly. These finding suggest the usefulness of CPP in the prevention of postmenopausal bone loss by decreasing urinary Ca excretion and bone resorption. Over 2 percent of CPP in the diet was effective to prevent postmenopausal bone loss.
Keywords
casein phophopeptides; calcium; phosphorus; bone biomarkers; ovariectomized rats;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Weaver CM. The growing years and prevention of osteoporosis in later life. Proc Nutr Soc 59: 303-306,2000
2 Weaver CM, Teegarden D, Lyle RM, McCabe GP, McCabe LD, Proulx W, Kern M, Sedlock D, Anderson DD, Hillberry BM, Peacock M, Johnston CC. Impact of exercise on bone health and contraindication of oral contraceptive use in young women. Med Sci Sports Exerc 33: 873-880, 2001   DOI   ScienceOn
3 Ferraretto A, Gravaghi C, Fiorilli A, Tettamanti G. Casein-derived bioactive phosphopeptides: role of phosphorylation and primary structure in promoting calcium uptake by HT-29 tumor cells. FEBS Lett 551: 92-98, 2003   DOI   ScienceOn
4 Li Y, Tome D, Deesjeuz JF. Indirect effect of casein phosphopeptide on calcium absorption in rat ileum in vitro. Reprod Nutr Dev 29: 227-233, 1989   DOI
5 Yuan YV, Kitts DD. Confirmation of calcium absorption and femoral utilization in spontaneously hypertensive rats fed casein phosphopeptide supplemented diets. Nutr Res 11: 1257-1272, 1991   DOI   ScienceOn
6 Pasquali R, Casimirri F, Labate AM, Tortelli O, Pascal G, Anconetani B, Flamia R, Capelli M, Barbbara L. Body weight, fat distribution and menopausal status in women. Int J Obes Relat Metab Disord 18: 614-621, 1994
7 Saito Y, Lee YS, Kimura S. Minimum effective dose of casein phophopeptides (CPP) for enhancement of calcium absorption in growing rats. Int J Vit Nutr Res 68: 335-340, 1998
8 Heaney RP, Saito Y, Orimo H. Effect of casein phosphopeptides on absorbability of co-ingested calcium in normal postmenopausal women. J Bone Min Metabol 12: 77-81, 1994   DOI
9 Sakhaee K, Nicar MJ, Class K, Pak CY. Postmenopausal osteoporosis as a manifestation of renal hypercalciuria with secondary hyperparathyroidism. J Clin Endocrinol Metab 61: 368-373, 1985   DOI
10 Pointillart A, Gueguen L. Absence of effect of the incorporation of a milk phosphopeptide on the utilization of calcium and phosphorus in young pig. Repro Nutr Dev 29: 477-486, 1989   DOI
11 Lee HJ, Lee HO. Calcium metabolism of menopausal women. Bulletin Home Culture 10: 145-169, 1997
12 Kasai T, Iwasaki R, Tanaka M, Kiriyama S. Caseinphosphopeptides (CPP) in feces and contents in digestive tract of rats fed casein and CPP preparations. Biosci Biotechnol Biochem 59 (1): 26-30, 1995   DOI   ScienceOn
13 Meisel H, Frister H. Chemical characterization of bioactive peptides from in vivo digest of casein. J Dairy Res 56: 343-349, 1989   DOI   ScienceOn
14 Narva M, Karkkainnen M, Poussa T, Lamberg-allardt C, Korpela R. Caseinphosphopeptides in milk and fermented milk do not affect calcium metabolim acutely in postmenopausa women. J Am Coll Nutr 22: 88-93, 2003   DOI
15 Prentice A. Is nutrition important in osteoporosis? Proc Nutr Soc 56: 357-367, 1997
16 Lindsay R. Sex steroids in the pathogenesis and prevention of osteoporosis, in: Riggs B, Melton LI (eds.), Osteoporosis: Etiology, Diagnosis and Management, Raven Press, New York, 1988
17 Kalu DK, Liu CC, Hardin R, Hollis BW. The aged rat model of ovarian hormone deficiency bone loss. Endocrinology 124: 7-16, 1989   DOI   ScienceOn
18 Rossouw JE, Anderson GL, Prentice RL, LaCroix AZ, Kooperberg C, Stefanick ML, Jackson RD, Beresford SA, Johnson KC, Howard BV, Kotchem JM, Ockene J. Writing Group for the Women's Health Initiative Investigators. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: Principal results from the Women' Health Initiative randomized controlled trial. JAMA 288: 321-333, 2002   DOI   ScienceOn
19 Meisel H. Biochemical properties of bioactive peptides derived from milk proteins: Potential nutraceuticals for food and pharmaceutical applications. Livest Prod Sci 50: 125-138, 1997   DOI   ScienceOn
20 Lee YS, Noguchi T, Naitio H. Intestinal absorption of calcium in rats given diets containing casein or amino acid mixture: the role of casein phosphopeptides. Br J Nutr 49: 67-76, 1983   DOI   ScienceOn
21 Nordin BE, Need AG, Morris HA, Horowitz M, Robertson WG. Evidence for a renal calcium leak in postmenopausal women. J Clin Endocrinol Metab 72: 401-407, 1991   DOI
22 Berrocal R, Chanton S, Juillerat MA, Pavillard B, Scherz JC, Jost R. Tryptic phosphopeptides from whole casein II. Physicochemical properties related to the solubilization of calcium. J Dairy Res 56: 335-341, 1989   DOI   ScienceOn
23 Meisel H, Frister H. Chemical characterization of a caseinophosphopeptide isolated from in vivo digests of a casein diet. Biol Chem Hoppe Seyler 369: 1275-1279, 1988   DOI
24 Tsuchita H, Suzuki T, Kuwata T. The effect of casein phosphopeptides on calcium absorption from calcium-fortified milk in growing rats. Br J Nutr 85: 5-10, 2001   DOI   ScienceOn
25 Shah NP. Effects of milk-derived bioactives: an overview. Br J Nutr 84: S3-S10, 2000
26 Scholz-Ahrens KE, Kopra N and Barth C.A. Effect of casein phosphopeptides on utilization of calcium in minipigs and vitamin-D-deficient rats. Z Ernahrungswiss 29: 295-298, 1990   DOI
27 Bennett T, Desmond A, Harringon M, McDonagh D, FitzGerld R, Flynn A, Cashman KD. The effect of high intakes of casein and casein phosphopeptide on calcium absorption in the rat. Br J Nutr 83: 673-680, 2000   DOI   ScienceOn
28 Albright F, Smith PH, Richardson D. Postmenopausal osteoporosis its clinical features. JAMA 116: 2465-2474, 1941   DOI
29 Watkins BA, Reinwald S, Li Y, Seifert MF. Protective actions of soy isoflavones and n-3 PUFA on bone mass in ovariectomized rats. J Nutr Biochem 16: 479- 88. 2005   DOI   ScienceOn
30 Lee YS, Noguchi T, Naito H. Phosphopeptides and soluble calcium in the small intestine of rats given a casein diet. Br J Nutr 43: 457-67, 1980   DOI   ScienceOn
31 Hansen M, Sandstrom B, Lonnerdal B. The effect of casein phosphopeptides on zinc and calcium absorption from high phytate infant diets assessed in rat pups and Caco-2 cells. Pediatr Res 40: 547-52, 1996
32 Sato R, Noguchi T, Naito H. Casein phosphopeptide (CPP) enhance calcium absorption from ligated segment of rat small intestine. J Nutr Sci Vitam 32: 67-76, 1986   DOI
33 Tsuchita H, Goto T, Shimizu T, Yonehara Y, Kuwata T. Dietary casein phophopeptides prevent bone loss in aged ovariectomized rats. J Nutr 126: 86-93, 1996   DOI
34 Guerrero R, Diaz Martin MA, Diaz Diego EM, Disla T, Rapado A, de la Piedra C. New biochemical markers of bone resorption derived from collagen breakdown in the study of postmenopausal osteoporosis. Osteoporos Int 6: 297-302, 1996
35 Wronski TJ, Cintron M, Dann LM. Temporal relationship between bone loss and increased bone turnover in ovariectomized rats. Calcif Tissue Int 43: 179-183, 1988   DOI
36 Wronski TJ, Walsh CC, lgnaszewski LA. Histologic evidence for osteopenia and increased bone turnover in ovariectomized rats. Bone 7: 119-123, 1986   DOI   ScienceOn
37 Bilezikian JP. Estrogens and postmenopausal osteoporosis: was Albright right after all? J Bone Miner Res 13: 774-776, 1998   DOI   ScienceOn
38 Mykkanen HM, Wasserman RH. Enhanced absorption of calcium by casein phosphopeptide in rachitic and normal chikcs. J Nutr 110:2141-2148, 1980   DOI
39 Allen LH. Calcium bioavailability and absorption: a review. Am J Clin Nutr 35: 783-808, 1982   DOI
40 Garnero P, Gineyts E, Riou JP, Delmas PD. Assessment of bone resorption with a new marker of collagen degradation in patients with metaolic bone disease. J Clin Endocrinol Metab 79: 780- 785, 1994   DOI
41 Erba D, Ciappellano S, Testolin G. Effect of the ratio of casein phosphopeptides to calcium (w/w) on passive calcium transport in the distal small intestine of rats. Nutr 18: 743-746, 2002   DOI   ScienceOn
42 Kalu DN, Lin CC, Salerno E, Hollis BW, Echon R, Ray M. Skeletal response of ovariectomized rats to low and high doses of 17${\beta}$-estradiol. Bone Miner 14: 175-187, 1991   DOI   ScienceOn
43 WHO/FAO Expert Consultation diet nutrition and the prevention of chronic diseases. WHO Technical Report Series, pp.916, 2003
44 Sato R, Shindo M, Gunshin H, Noguchi T, Naito H. Characterization of phosphopeptide derived from bovine ${\beta}$-casein: an inhibitor to intra-intestinal precipitation of calcium phosphate. Biochem Biophys Acta 1077: 413-415, 1991