• Applied Biological Chemistry
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• v.39 no.4
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• pp.268-273
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• 1996

Fundamental data for new type of product development from Lycii fructus was experimentally determined. The chemical composition, and Hunter value changes, optimum extraction, volatile components of extract under different extraction conditions and heat treatment were mainly studied. Results are summarized as follows. The proximate compositions of dried Lycii fructus were water 21.8%, total sugar 27.6%, reducing sugar 15.2%, crude protein 14.29%, crude fat 5.65%, crude fiber 7.48%, and ash 7.98% in percent stale, respectively. Extracted yield on the basic of solid extract was getting increased when more solvent was used for extraction. The most recommendable extraction was 1 to 10 part of sample to solvent ratio. When water was employed as extraction solvent, the highest amount of solid extract was obtained. Extract of Lycii fructus in terms of yields and color was most acceptable when raw sample was treated 8 minute roasting at $180^{\circ}C$ and 60 minute heating at $120^{\circ}C$, respectively. Major volatile components of fresh Lycii fructus were to hexadecanoic acid, methyl linoleate, benzyl alcohol, dimetane benzene by GC/MS. By the roasting of raw sample, the compounds of 2-methyl-2buthenal, 1,4-dimethyl benzene, and benzyl alcohol were reduced. Wheras, methyl thiopropanol, benzene acetaldehyde and ethyl linoleate were slightly increased.

• Journal of Naturopathy
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• v.10 no.2
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• pp.86-92
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• 2021

Background: The presence of antioxidants was not confirmed in the medicinal plant Equisetum hyamale grass. Purposes: This study was to determine the antioxidant and elastase inhibition effects of extracts of E. hyemale. Methods: Antioxidant functions of E. hyemale stems and roots were measured and extracted with hot water (HW) and ethyl alcohol (70EOH, 100EOH). Results: The extraction yield of stems was higher in HW extraction than in ethyl alcohol extraction. The polyphenol content was significantly higher in the root extract than in the stem. Total flavonoid content of 70EOH extract was significantly higher in root extract than in stem. The elastase inhibitory function of the extract was 46% in the root and 49% in the stem at 100 ppm of the extract. The ABTS free radical scavenging function was in the order of HW<70EOH<100EOH

• YAKHAK HOEJI
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• v.34 no.4
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• pp.282-286
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• 1990

An hydrocarbon alcohol and steroids were isolated from the hexane fraction of the extract from the seeds of Phaseolus radiatus L. And also a flavonoid was identified from the ethyl acetate and butanol fraction. The structure of the obtained compound were elucidated by spectroscopic methods (UV, IR, mass and NMR) and their physicochemical properties. As the results, triacontanol, stigmasterol, ${\beta}-sitosterol$ and vitexin were identified.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.532-536
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• 2005

In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide ($10\;{\mu}g/ml$), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with $IC_{50}$ value of $3.0\;{\mu}g/ml$ after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.

• Korean Journal of Agricultural Science
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• v.4 no.2
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• pp.121-125
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• 1977

The activity of endogenous cytokinin-like substance in dormant plants(Fragaria ananassa) was different depending on the degree of dormancy and their activity was showed the similar trend as that of growth phase. When strawberry plant crowns were extracted in methyl alcohol, and the extract was seperated by column chromatography, it showed that the activity was observed in two places by soybean callus bioassay. Activity of cytokinin-like substance was appeared when ethyl acetate/methyl alcohol (90/10v. and 50/50v.) were used. In relation to growth phase of strawberry plants, cytokinin-like activity increased at vigorous growth periods, whereas decreased in deep dormant period. However, cytokinin-like substance activity occurred again after strawberry plants passed through a certain chilling periods. Accordingly, it showed to have a negative correlation between the degree of dormancy and a mount of endogenous cytokinin-like substance.

• Journal of environmental and Sanitary engineering
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• v.20 no.3 s.57
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• pp.10-16
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• 2005

Chestnut bark extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. $10{\mu}g/ml{\ell}\;100{\mu}g/m{\ell}$, $1mg/m{\ell}$ of the extract repressed expression of tyrosinase gene about $38\%,\;47\%,\;and\;78\%$, respectively, compared with control. In the MTT assay, the same extract exhibited very low cytotoxicity at $1{\mu}g/m{\ell},\;10{\mu}g/m{\ell},\;100{\mu}g/m{\ell},\;and\;1mg/m{\ell}$, respectively. The fractions of Methylene chloride and ethyl acetate did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of butyl alcohol repressed highly at $10{\mu}g/m{\ell}\;and\;100{\mu}g/m{\ell}$.

• Journal of environmental and Sanitary engineering
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• v.21 no.3 s.61
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• pp.44-51
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• 2006

To estimate the inhibitory effect of Rubus coreanum extract on melanin biosynthesis, we tested its inhibitory effects on tyrosinase promoter in B16 mouse melanoma cells. Rubus coreanum extract by methanol had inhibitory effect approximately 10% on tyrosinase promoter at $100{\mu}g/mL$. In the MTT assay, the same extract exhibited very low cytotoxicity under $100{\mu}g/mL$. The fractions of methylene chloride, butyl alcohol and water did not showed the inhibitory effect on tyrosinase promoter, but the fraction of ethyl acetate exhibited inhibitory effect approximately 11% at $100{\mu}g/mL$.

• Textile Coloration and Finishing
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• v.22 no.1
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• pp.21-27
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• 2010

The purpose of this research was to investigate the color characteristic and the dyeing properties of Glycyrrhizae Radix extract on cotton fabrics. The highest absorbance of Glycyrrhizae Radix extract was obtained in 75 v/v% of ethyl alcohol in water. Pre-mordanted cotton fabrics dyed at $30^{\circ}C$ and pH 3 for 80 minutes showed the highest K/S value. According to the dyeing conditions, the colors of the fabrics varied from yellow(Y) and yellowish red(YR) to greenish yellow(GY). The color fastness of the fabrics in dry-cleaning and water was 4~5 rating. The deodorization capacity and UV-cut effect of the dyed fabrics were higher than those of undyed ones.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.3
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• pp.1031-1038
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• 2012

Background: Turmeric ($Curcuma$ $longa$) has been shown to possess anti-inflammatory, antioxidant and antitumor properties. However, despite the progress in research with $C.$ $longa$, there is still a big lacuna in the information on the active principles and their molecular targets. More particularly very little is known about the role of cell cycle genes $p57^{kip2}$ and Rad9 during chemoprevention by turmeric and its derivatives especially in prostate cancer cell lines. Methods: Accordingly, in this study, we have examined the antitumor effect of several extracts of $C.$ $longa$ rhizomes by successive fractionation in clonogenic assays using highly metastatic PC-3M prostate cancer cell line. Results: A mixture of isopropyl alcohol: acetone: water: chloroform: and methanol extract of $C.$ $longa$ showed significant bioactivity. Further partition of this extract showed that bioactivity resides in the dichloromethane soluble fraction. Column chromatography of this fraction showed presence of biological activity only in ethyl acetate eluted fraction. HPLC, UV-Vis and Mass spectra studies showed presence three curcuminoids in this fraction besides few unidentified components. Conclusions: From these observations it was concluded that the ethyl acetate fraction showed not only inhibition of colony forming ability of PC-3M cells but also up-regulated cell cycle genes $p57^{kip2}$ and Rad9 and further reduced the migration and invasive ability of prostate cancer cells.

• Korean Chemical Engineering Research
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• v.53 no.6
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• pp.717-722
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• 2015

D-limonene included in citrus fruits is obtainable to extract essential oil as well as separate the oil ingredient. Soxhlet extraction, a type of SDE (Simultaneous steam Distillation and solvent Extraction), was used to extract limonene from tangerine peel. HPLC analysis was performed to quantify extracted d-limonene by using reversed-phase HPLC column. Results of HPLC analysis showed that the optimal extraction time was 2 hours in any solvent, and the extracted amounts of d-limonene in tangerine peel (per g tangerine peel) were 7.77 mg, 0.49 mg, and 0.28 mg in ethyl alcohol, n-hexane, and ether. Because yield was the highest in using ethyl alcohol as a solvent, polarity is stronger factor to effect on yield of extraction than boiling point.