Browse > Article
http://dx.doi.org/10.9713/kcer.2015.53.6.717

Optimization of d-limonene Extraction from Tangerine Peel in Various Solvents by Using Soxhlet Extractor  

Park, Sang Min (Department of Chemical Engineering, Chungnam National University)
Ko, Kwan Young (Department of Chemical Engineering, Chungnam National University)
Kim, In Ho (Department of Chemical Engineering, Chungnam National University)
Publication Information
Korean Chemical Engineering Research / v.53, no.6, 2015 , pp. 717-722 More about this Journal
Abstract
D-limonene included in citrus fruits is obtainable to extract essential oil as well as separate the oil ingredient. Soxhlet extraction, a type of SDE (Simultaneous steam Distillation and solvent Extraction), was used to extract limonene from tangerine peel. HPLC analysis was performed to quantify extracted d-limonene by using reversed-phase HPLC column. Results of HPLC analysis showed that the optimal extraction time was 2 hours in any solvent, and the extracted amounts of d-limonene in tangerine peel (per g tangerine peel) were 7.77 mg, 0.49 mg, and 0.28 mg in ethyl alcohol, n-hexane, and ether. Because yield was the highest in using ethyl alcohol as a solvent, polarity is stronger factor to effect on yield of extraction than boiling point.
Keywords
Tangerine Peel; d-limonene; Soxhlet Extraction; HPLC;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Morse, M. A. and Toburen, A. L., "Inhibition of Metabolic Activation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone by Limonene," Cancer Lett., 104(2), 211-217(1996).   DOI
2 Budavari, S., The Merck Index, 7th ed., Merck, New Jersey(1989).
3 Langer, R. S. and Wise, D. L., Medical Applications of Controlled Release, 2nd ed., CRC Press, Florida(2004).
4 Rosen, H. B., Chang, J., Wnek. G. E., Linhardt, R. J. and Langer, R., "Bioerodible Polyanhydrides for Controlled Drug Delivery," Biomaterials, 4(2), 131-133(1983).   DOI
5 Oh, E. H., Im, H. S. and Yoon, C. H., "A Study on the Antibiotic Effect Using the d-limonene Oil Extracted to Wasted Mandarin Peels in Cheju," Journal of the Korean Oil Chemists' Society, 26(3), 350-356(2009).
6 Beak, J. I., Jin, S. Y. and Kim, I. H., "Separation of $\alpha$-lactalbumin and $\beta$-lactoglobulin by Using HPLC," B. S. Thesis, Chungnam National University, Daejeon, Korea(2009).
7 Wixom, R. L., Chromatography, Wiley Press, New York(2011).
8 Jung, I. K., Choi, D. Y. and Row, K. H., "Separation of Whey Proteins by Anion-exchange Membranes," Korean J. Chem. Eng., 20(3), 538-541(2003).   DOI
9 Nilsson, U., Bergh, M., Shao, L. P. and Karlberg, A-T., "Analysis of Contact Allergenic Compounds in Oxidized d-limonene," Chromatographia, 42(3-4), 199-205(1996).   DOI
10 Zhang, R. L. Zhang, Q. and Xu, S. A., "Determination of Limonene in Spearmint Oil by HPLC Method," Journal of Anhui Agricultural Sciences, 13(2), 743-744(2012).
11 http://www.sanderkok.com/techniques/hplc/eluotropic_series_extended.html.
12 Lee, K. J., Choi, D. Y. and Row K. H., "Extraction and Purification of Isoflavones from Korean Soybean and Soybean Paste," Korean Chem. Eng. Res., 41(5), 612-616(2003).
13 Yoon, C. W., Bok, H. S., Choi, D. K. and Row, K. H., "Optimization Condition of Astaxanthin Extract from Shrimp Waste Using Response Surface Methodology," Korean Chem. Eng. Res., 50(3), 545-550 (2012).   DOI
14 Im, H. H., "Studies on Extraction of Limonene from Citron and Immunomodulation Activity of Limonene," M.S. Thesis, Hankyong National University, Ansung, Korea(2005).