• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.2
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• pp.154-159
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• 2013

The AOAC Mouse Bioassay method (MBA) has been widely used for routine monitoring of paralytic shellfish poisoning (PSP) for more than 50 years. However, this method has low sensitivity and experiences interference from other components in the extract. Also, ethical issues have been raised against the continued use of this live-mouse assay. To establish an alternative method for PSP analysis, we attempted to develop PSP analysis conditions using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS analysis of reference material showed very reasonable accuracy, and the analysis time was just 15 min. However, the recovery rate of toxin spike samples using the LC-MS/MS analysis was 59.4-91.0%. We also attempted to remove the matrix effect using shellfish extracts, but recoveries of C1 and C2 did not improve. A comparison between the results of MBA and LC-MS/MS analysis revealed good correlations, with values of 0.8878 and 0.9211 for oyster and mussel matrices, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.2
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• pp.147-153
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• 2013

To prevent paralytic shellfish poisoning (PSP) due to the consumption of shellfish contaminated with PSP toxins, the quantitative analysis of these toxins is very crucial. The AOAC International mouse bioassay (MBA) has been used widely for the routine monitoring of PSP toxins for more than 50 years. However, this method has low sensitivity and high limit of quantification (LOQ) and interferences from other components in the extract, and it cannot determine toxic profiles. Ethical problems also exist with the continued use of this live mouse assay. To establish an alternative method to the MBA used for PSP toxins analysis, we attempted to optimize the analysis conditions of a precolumn high-performance liquid chromatography (HPLC) oxidation method and succeeded in validating its accuracy and precision in quantifying PSP toxins. A clear peak and the isolation of PSP toxins were obtained by injecting the working standards of Certified Reference Materials using HPLC. The LOQ of the precolumn HPLC oxidation method for PSP toxins was about $0.1002{\mu}g/g$, which represented an approximately fourfold improvement in detection capability versus the AOAC MBA. The intra-accuracy and precision for PSP toxins in oysters were 77.0-103.3% and 2.0-5.7%, respectively, while the respective inter-accuracy and precision were 77.3-100.7% and 2.4-6.0%. The mean recoveries of PSP toxins from oysters were 75.2-112.1%. The results of a comparison study showed good correlation between the results of the precolumn HPLC oxidation method and those of MBA, with a correlation factor of 0.9291 for mussels. The precolumn HPLC oxidation method may be used as an alternative to, or supplementary method with, MBA to monitor the occurrence of PSP toxins and to analyze the profiles of these toxins in shellfish.

• The Journal of the Korea Contents Association
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• v.7 no.11
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• pp.140-149
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• 2007

Social recognition conversion of the jewelry-related industries must be preceded by changes of responsibility of both the staff in charge and educators in education objections. Based on the debate of whether or not increasing (public) interest in jewelry design will subsequently lead to bringing about vision and possibility in the form of cultural management, this paper examined the influences that logical systematization of jewelry design and educational process of jewelry designers' ethics have on the next generation of the cultural industry of jewelry. Through the education and the industry of Italy, this paper analyzed existent method of design, current trend, and new content sand cases of design management that are based on types of consumption. In addition, it also provided the developmental possibility of future culture industry, and the foundation of ethical philosophy of jewelry education that lies beyond national economic power. I intend to imply significance of how designer's perception and conduct provides the whole new conventional foundation of sympathy and sensitivity and the existent method of design.

• Hwankyungkyoyuk
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• v.12 no.1
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• pp.150-171
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• 1999

This study is aimed to systematize the contents of environmental education in the home education and to develop the environmental education. For this, this study firstly examined the characteristics and goals of home education and its relation to environmental education. Secondly, in the paradigm of environmental education this study analyzed the goals and contents of environmental education in the domain of home education which is included in the practicum subject of the 7th curriculum. Thirdly, this study examined the effective teaching and learning methods for home education and the considerations in organization of textbook contents which reflect these teaching and learning methods. Finally, this study suggested an example of textbooks which reflect all these considerations. It has been found out that: (1) the home education is based on the recognition of environmental problems related to home life, (2) the home education explains the environmental problems, and (3) the home education has its goals of acquring active attitudes and skills to solve these problems. In the teaching contents, the home education was analyzed to have the following concerning environmental education: the utilization of resources for food, clothes, and housing and home life, that is, the domain of ‘environmental sanitation’ related to food ingestion and sanitary life, the domain of ‘sound consumption life’ related to the utilization of resources and their consumption, and the domain of ‘environmental pollution’ on the prevention of every kind of pollutants in the home life. However, the environmental education in the home education according to the 7th educational curriculum has the distinctiveness from the contents of environmental education which were emphasized in the past home education. The distinctiveness are as following: (1)the resources matter is dealt with in the aspect of recycling various resouces from home life, (2)the prevention or reduction of pollutants in life which take the considerable part of environmental pollution is emphasized, (3)children's sensitivity In environment is emphasized to be developed, and (4)the importance of life is emphasized to be taught.

• The Journal of the Convergence on Culture Technology
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• v.4 no.1
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• pp.207-217
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• 2018

The purpose of this study was to identify nursing research trends for the consciousness of bioethics published in korea. A total of 30 studies were analyzed using descriptive statistics. 70% of the studies was published for the last five years, and 76.7% of studies were conducted in a descriptive study. Nursing students accounted for 83.1% in terms of the participants, and a questionnaire was used for the data collections in the entire studies. The studies considered ethical aspect accounted for 90.0%. In the experimental studies, the nursing intervention was a education program, and dependent variables of the studies included consciousness of bioethics, moral sensitivity, critical thinking and moral judgment. Among the top 10 priorities in the key words, the first priorities was the bio-medical ethics. In conclusion, it is suggested to develop an expanding-iterative studies, qualitative studies, and development of bioethics programs for nurses.

• Nutrition Research and Practice
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• v.13 no.1
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• pp.41-46
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• 2019

BACKGROUND/OBJECTIVES: Taste perception is influenced by both nutritional factors and psychological factors. This study was undertaken to measure the 4 basic taste perceptions, nutrient intake, and mental health, and to examine the factors that affect insensitive taste perception in young Japanese women. SUBJECTS/METHODS: Young women in their late teens and twenties were enrolled as subjects. Taste perception was measured by applying the filter-paper disc method over areas of the chorda tympani nerve. Nutritional status was evaluated using brief, self-administered diet history questionnaires. The index of nutritional status was based on the 2015 Japanese dietary reference intakes. Mental health was assessed using the Japanese translation of the Profile of Mood States short version. This study was approved by the ethical committee at Osaka University. RESULTS: The normal taste perception group (four basic tastes [sweet, salty, sour, and bitter] identified as normal taste perception) comprised 55.4% of the subjects, while the abnormal taste perception group (more than 1 abnormal taste perception was perceived, regardless of flavor) comprised 44.6% of the subjects. There were no significant differences in nutrient intake (except manganese) and mental health between the normal and abnormal taste perception groups. Subjects who took 5 mg to less than 7.1 mg zinc per day were at significantly decreased risk of insensitive taste perception compared to subjects who consumed less than 5 mg zinc per day [Regression coefficient 0.831, 95% confidence interval 0.694-0.996]. CONCLUSION: The present results suggest that insensitive taste perception could be associated with zinc deficiency in young women in their late teens and twenties.

• Clinical and Experimental Reproductive Medicine
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• v.46 no.1
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• pp.14-21
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• 2019

Objective: The usual seminal profile has been customarily used for diagnosing male infertility based on an examination of semen samples. However, sperm DNA fragmentation has also been causally linked to reproductive failure, suggesting that it should be evaluated as part of male infertility assessments. To compare the ability of the five most widely utilized methodologies of measuring DNA fragmentation to predict male infertility and reactive oxygen species by Oxisperm kit assay. Methods: In this case-control study, which received ethical committee approval, the participants were divided into fertile and infertile groups (50 patients in each group). Results: The alkaline comet test showed the best ability to predict male infertility, followed by the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay, the sperm chromatin dispersion (SCD) test, and the sperm chromatin structure assay (SCSA), while the neutral comet test had no predictive power. For our patient population, the projected cut-off point for the DNA fragmentation index was 22.08% using the TUNEL assay, 19.90% using SCSA, 24.74% using the SCD test, 48.47% using the alkaline comet test, and 36.37% using the neutral comet test. Significant correlations were found between the results of the SCD test and those obtained using SCSA and TUNEL (r = 0.70 and r = 0.68, respectively; p< 0.001), and a statistically significant correlation was also found between the results of SCSA and the TUNEL assay (r = 0.77, p< 0.001). Likewise, the results of the alkaline comet test showed significant correlations with those of the SCD, SCSA, and TUNEL tests (r = 0.59, r = 0.57, and r = 0.72, respectively; p< 0.001). Conclusion: The TUNEL assay, SCSA, SCD, and the alkaline comet test were effective for distinguishing between fertile and infertile patients, and the alkaline comet test was the best predictor of male infertility.

• Journal of Animal Science and Technology
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• v.66 no.1
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• pp.31-56
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• 2024

Pig farming, a vital industry, necessitates proactive measures for early disease detection and crush symptom monitoring to ensure optimum pig health and safety. This review explores advanced thermal sensing technologies and computer vision-based thermal imaging techniques employed for pig disease and piglet crush symptom monitoring on pig farms. Infrared thermography (IRT) is a non-invasive and efficient technology for measuring pig body temperature, providing advantages such as non-destructive, long-distance, and high-sensitivity measurements. Unlike traditional methods, IRT offers a quick and labor-saving approach to acquiring physiological data impacted by environmental temperature, crucial for understanding pig body physiology and metabolism. IRT aids in early disease detection, respiratory health monitoring, and evaluating vaccination effectiveness. Challenges include body surface emissivity variations affecting measurement accuracy. Thermal imaging and deep learning algorithms are used for pig behavior recognition, with the dorsal plane effective for stress detection. Remote health monitoring through thermal imaging, deep learning, and wearable devices facilitates non-invasive assessment of pig health, minimizing medication use. Integration of advanced sensors, thermal imaging, and deep learning shows potential for disease detection and improvement in pig farming, but challenges and ethical considerations must be addressed for successful implementation. This review summarizes the state-of-the-art technologies used in the pig farming industry, including computer vision algorithms such as object detection, image segmentation, and deep learning techniques. It also discusses the benefits and limitations of IRT technology, providing an overview of the current research field. This study provides valuable insights for researchers and farmers regarding IRT application in pig production, highlighting notable approaches and the latest research findings in this field.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.11
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• pp.5773-5775
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• 2012

Objective: To assess the diagnostic and prognostic value of AFP and des-gamma-carboxyprothrombin (DCP) in combination and alone for hepatocellular carcinoma. Materials and Methods: A case control study carried out in the Department of Biochemistry of Manipal College of Medical Sciences, Pokhara, Nepal between $1^{st}$ January 2010 and $31^{st}$ December 2011. The variables collected were age, gender, BMI, total proteins, albumin, AST, ALT, total bilirubin, DCP, AFP. Approval for the study was obtained from the institutional research ethical committee. Estimation of AFP was performed by ELISA reader for all cases. Analysis was done using descriptive statistics and confidence interval (CI). The data was analyzed using Excel 2003, R 2.8.0 Statistical Package for the Social Sciences (SPSS) for Windows Version 16.0 (SPSS Inc; Chicago, IL, USA) and the EPI Info 3.5.1 Windows Version. Results:The mean age of HCC cases was $53.6{\pm}14.93$ yrs. The percentage of females was less than males in both cases (23%) and controls (29%). The specificity of DCP reached 100% when its values was equal or greater than 150 (MAU/ml) for 0, 3, 6, 9, 12 months preceding the diagnosis of HCC. Similarly, the specificity for AFP was also nearly 100% when its value was equal or greater than 200 ng/ml 0, 3, 6, 9, 12 months earlier to the finding of HCC. The specificity of DCP (${\geq}40MAU/mL$) and AFP(${\geq}20$ ng/mL) in combination was 93%, 97%, 95%, 96%, 97% in respect to 0, 3, 6, 9, 12 months prior to the diagnosis of HCC. Conclusion: The combination of both DCP and AFP will improve the finding of initial HCC and the sensitivity of these markers was utmost at the time of HCC identification and noticeably lesser at former time points.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.7
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• pp.46-53
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• 2020

The purpose of this study was to examine the direction of social welfare education in the 4th Industrial Revolution as well as discuss the overall direction of social welfare education such as competency and curriculum and the educational dimension of social welfare professionals. Using Text Network Analysis, 223 studies published from 2005 to 2019 in the Korean Journal of Social Welfare Education were examined in order to explore the direction of social welfare education in the 4th Industrial Revolution. Using Word cloud, overall frequency was analyzed. As a result of key words analysis, social welfare education (43), research method (28), and social welfare field practice (23) were analyzed as influential key words. The directions of social welfare education in the 4th Industrial Revolution era are as follows. First, competency, curriculum, and qualifications are necessary in general social welfare education. Second, education centering on social workers and social welfare students, who are social welfare professionals, is necessary. Third, the ethical sensitivity of future social welfare should be carefully established. Finally, the need for a shared welfare system must be further studied.