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http://dx.doi.org/10.5657/KFAS.2013.0154

Paralytic Shellfish Poisoning (PSP) Analysis using Liquid Chromatography-Tandem Mass Spectrometry  

Song, Ki Cheol (West Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
Lee, Ka Jeong (Food Safety Research Division, National Fisheries Research & Development Institute)
Yu, Hong Sik (Food Safety Research Division, National Fisheries Research & Development Institute)
Mok, Jong Soo (Southeast Sea Fisheries Institute, National Fisheries Research & Development Institute)
Kim, Ji Hoe (Food Safety Research Division, National Fisheries Research & Development Institute)
Lim, Keun Sik (Food Safety Research Division, National Fisheries Research & Development Institute)
Lee, Mi Ae (Food Safety Research Division, National Fisheries Research & Development Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.46, no.2, 2013 , pp. 154-159 More about this Journal
Abstract
The AOAC Mouse Bioassay method (MBA) has been widely used for routine monitoring of paralytic shellfish poisoning (PSP) for more than 50 years. However, this method has low sensitivity and experiences interference from other components in the extract. Also, ethical issues have been raised against the continued use of this live-mouse assay. To establish an alternative method for PSP analysis, we attempted to develop PSP analysis conditions using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS analysis of reference material showed very reasonable accuracy, and the analysis time was just 15 min. However, the recovery rate of toxin spike samples using the LC-MS/MS analysis was 59.4-91.0%. We also attempted to remove the matrix effect using shellfish extracts, but recoveries of C1 and C2 did not improve. A comparison between the results of MBA and LC-MS/MS analysis revealed good correlations, with values of 0.8878 and 0.9211 for oyster and mussel matrices, respectively.
Keywords
Paralytic Shellfish Poisoning (PSP); LC-MS/MS; MBA (Mouse Bioassay);
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