• Journal of Oriental Neuropsychiatry
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• v.20 no.3
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• pp.1-13
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• 2009

Objectives : The glial cell, located in between the blood vessel and nerve cell, takes charge of the cell support, nutrition supply, elimination of body waste, and cell action. GagamSohabhwangwon(GGSH), a chinese traditional medicinal prescription has been used orally for the treatment of seizures, infantile, convulsion, stroke and so forth. This paper examines the effect of the GagamSohabhwangwon(GGSH) essential oil on cell activity and anti oxidation. Methods : MTT assay methods were employed to measure the cell activity based on the amount of the GagamSohabhwangwon(GGSH) essential oil by using primarily cultivated glial cell. In addition, this paper measured a viability of the glial cell after a protein active retarder control to confirm the multiplication of the cell and examined the cell extinction by the active oxygen, an extinction shielding effect with different amount of the GagamSohabhwangwon(GGSH) essential oil to observe anti oxidation. Furthermore, this paper measured a viability of the cell and phosphorylation(phosphorylation) of the protein which affects the multiplication of the glial cell. Results : When controlling the amount of the GagamSohabhwangwon(GGSH), there was a multiplication effect of the primary glial cell, the multiplication of the cell was dependent on the density of the GagamSohabhwangwon. The multiplication power of the primary glial cell was suppressed by PKA inhibiter (H89). In compliance with the active oxygen the extinction of the primary glial cell was dependent on the density of the GagamSohabhwangwon, there was a shielding effect of the cell extinction when GagamSohabhwangwon(GGSH) was preprocessed. When inducing the multiplication of the primary glial cell, phosphorylation of the Akt, BDNF, CREB, ERK and ERM were increased. Conclusions: Based on the results, GagamSohabhwangwon essential oil will have the effect which activates the nervous system cell and protects the cell through anti oxidation.

• Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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• v.5
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• pp.167-182
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• 2002

Sprachverstehen ist ein interaktiver kognitiver Prozess. Der Verlauf des Sprachverstehens ist durch die kontinuierliche Interaktion von Bottom-up- und Top­down-Prozessen gekennzeichnet. Die eingehende Information wird durch die interaktive Nutzung verschiedener sprachlicher und aussersprachlicher Wissenssysteme verarbeitet. Herangewgen wird z. B. syntaktisches Wissen, lexikalisches und semantisches Wissen, Weltwissen und pragmatisches Wissen. Das hier vorgestellte Modell stellt den Begriff des lexikalischen Kopfes als $f\"{u}r$ die Verarbeitung von $S\"{a}tzen$ zentrales Konzept heraus. $Ambiguit\"{a}ten$ werden unter $Ber\"{u}cksichtigung$ der in ihnen $repr\"{a}sentierten\;Information\;aufzul\"{o}sen$ versucht Im Unterschied zu Prinzipien wie 'minimal attachment' rekurriert 'head attachment' nicht auf eine dem Satz zugrundeliegende Struktur, sondem allein auf lexikalische Information von $K\"{o}pfen$ und deren Reihenfolge an der $Oberfl\"{a}che$ eines Satzes. Head attachment ist daher $unabh\"{a}ngig$ von Annahmen $\"{u}ber$ syntaktische Strukturen. $W\"{a}hrend$ der 'tiefere Sinn' von Prinzipien wie 'minimal attachment' darin liegen soll, eine $m\"{o}glichst$ geringe Arbeitsspeicherbelastung durch Aufbau $m\"{o}glichst$ wenig komplexer Strukturen zu $gew\"{a}hrleisten$, erreicht 'head attachment' eine Entlastung durch die $Erm\"{o}glichung\;einer\;fr\"{u}hen$ semantischen Integration, da es Anbindungen in Abh\"{a}ngigkeit$ von dem Vorhandensein von $K\"{o}pfen\;vorschl\"{a}gt$. Es ist dieser Aspekt $\"{o}konomischer$ Verarbeitung, durch Verarbeitungs­heuristiken zu einer $m\"{o}glichst\;fr\"{u}hzeitigen$ Interpretation zu gelangen, der im hier vorgestellten Modell im Vordergrund steht, und zwar im Sinne einer semantikorientierten Syntaxverarbeitung.

• Korean Journal of Microbiology
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• v.51 no.3
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• pp.256-262
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• 2015

In order to study the effect of the receptor protein (SeaR), which is isolated from Saccharopolyspora erythraea, we introduced the SeaR gene to Streptomyces virginiae as host strains. An effective transformation procedure for S. virginiae was established based on transconjugation by Escherichia coli ET12567/pUZ8002 with a ${\varphi}C31$-derived integration vector, pSET152, which contained int, oriT, attP, and $ermEp^{\ast}$ (erythromycin promotor). Therefore, the pEV615 was introduced into S. virginiae by conjugation and integrated at the attB locus in the chromosome of the recipients by the ${\varphi}C31$ integrase (int) function. Transformants of S. virginiae containing the SeaR gene were confirmed by PCR and transcriptional expression of the SeaR gene in the transformants was analyzed by RT-PCR, respectively. And, we examined the production time of virginiamycins in the culture media of both the transformants and the wild type. The production time of virginiamycins in the wild type and transformants was the same. When 100 ng/ml of synthetic $VB-C_6$ was added to the state of 6 or 8 hour cultivation of wild type and transformants, respectively, the virginiamycins production was induced, meaning that the virginiamycins production in the wild type was detected 2 h early than transformants. From these results, SeaR expression was also affected to virginiamycins production in transformants derived from S. virginiae. In this study, we showed that the SeaR protein worked as a repressor in transformants.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2013.06a
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• pp.227-230
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• 2013

Recently, IMO STW Committee has released a model course of Bridge Resource Management. The Leadership and Teamwork among non-technical skills related to human factor is included at STCW 2010 Manila Amendments. Until now, the BRM education in Korea has been independently operated by each related educational institution, i.e., Shipping Company, University due to absence of model course. In this research the focus is on the simulation module among items for the BRM program. During the simulation from the BRM training, the trainee's reaction and popular functions on simulator have been investigated and analyzed to develop a simulation module for effective BRM education.

• Korean Journal of Microbiology
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• v.51 no.1
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• pp.39-47
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• 2015

Secondary metabolism in actinomycetes has been known to be controlled by a small molecule, ${\gamma}$-butyrolactone autoregulator, the binding of which to each corresponding receptor leads to the regulation of the transcriptional expression of the secondary metabolites. We expected that expression of an autoregulator receptor or a pleiotropic regulator in a non-host was to be gained insight of effective production of new metabolic materials. In order to study the function of the receptor protein (seaR), which is isolated from Saccharopolyspora erythraea, we introduced the seaR gene to Streptomyces coelicolor A3(2) as host strains. An effective transformation procedure for S. coelicolor A3(2) was established based on transconjugation by Escherichia coli ET12567/pUZ8002 with a ${\varphi}C31$-derived integration vector, pSET152, which contained int, oriT, attP and $ermEp^*$ (erythromycin promotor). Therefore, the pEV615 was introduced into S. coelicolor A3(2) by conjugation and integrated at the attB locus in the chromosome of the recipients by the ${\varphi}C31$ integrase (int) function. Exconjugant of S. coelicolor A3(2) containing the seaR gene was confirmed by PCR and transcriptional expression of the seaR gene in the transformant was analyzed by RT-PCR. In case of S. coelicolor A3(2), a phenotype microarray was used to analyze the phenotype of transformant compared with wild type by seaR expression. After that, in order to confirm the accuracy of the results obtained from the phenotype microarray, an antimicrobial susceptibility test was carried out. This test indicated that sensitivity of the transformant was higher than wild type in tetracycline case. These results indicated that some biosynthesis genes or resistance genes for tetracycline biosynthesis in transformant might be repressed by seaR expression. Therefore, subsequent experiments, analysis of transcriptional pattern of genes for tetracycline production or resistance, are needed to confirm whether biosynthesis genes or resistance genes for tetracycline are repressed or not.

• Food Science of Animal Resources
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• v.36 no.3
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• pp.352-358
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• 2016

The aim of this study was to determine the prevalence of enterococci in cheese samples and to characterize their antimicrobial resistance profiles as well as the associated resistance genes. A total of 139 enterococci were isolated from 99 cheese samples, the isolates were identified as E. faecalis (61.2%), E. faecium (15.1%), E. gallinarum (12.9%), E. durans (5.0%), E. casseliflavis (2.9%) and E. avium (2.9%). The most frequent antimicrobial resistance observed in enterococci isolates was to lincomycin (88.5%), followed by kanamycin (84.2%), gentamycin (low level, 51.1%), rifampin (46.8%) and tetracycline (33.8%). Among the isolates, the frequencies of high level gentamycin and streptomycin resistant enterococci strains were 2.2% and 5.8%, respectively. Apart from the mentioned antibiotics, low levels of resistance to ciprofloxacin, erythromycin and chloramphenicol were found. Moreover no resistance was observed against penicillin and ampicillin. The antimicrobial resistance genes including tetM, tetL, ermB, cat, aph(3’)-IIIa, ant(6)-Ia and aac(6’)-Ieaph(2”)-Ia were found in enterococci from Turkish cheese samples. In the current study, we provided data for antibiotic resistance and the occurrence of resistance genes among enterococci. Regulatory and quality control programs for milk and other dairy products from farms to retail outlets has to be established and strengthened to monitor trends in antimicrobial resistance among emerging food borne pathogens in Turkey.

• Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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• v.8
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• pp.273-297
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• 2003

Der Computer kann ein nutzliches, herausforderndes und kreatives Werkzeug und Hilfsmittel beim Sprachenlernen darstellen. Bei der Anwendung auf die Situation des Grammatiklernens sind die analytischen und interaktiven $F\"{a}higkeiten$ des Computers mit gutem Erfolg eingesetzt worden. Eine der attraktivsten Eigenschaften des Computers ist nun seine $F\"{a}higkeit$, mit dem Schiller zu interagieren. Gute Computerprogramme $erm\"{o}glichen$ eine ausgedehnte Interaktion zwischen Computer und $Sch\"{u}ler$. Die Computerprogramme zum Sprachenlemen befassen sich mit Grammtik. Hier habe ich ein Lemprogramm des Intelligent Tutoring Systems $f\"{u}r$ die Grammatik Perfekt programmiert. Das Intelligent Tutoring System ist ein Computerlehrer. Wenn dem $Sch\'{u}ler$ Fehler unterlaufen, gibt ein guter Lehrer nicht nur die richtige Antwort, sondern hilft dem Schiller auch, seinen Fehler zu erkennen, ihn zu analysieren und zur richtigen Antwort zu finden. Das ist eine Funktion des Intelligent Tutoring Systems. Die Programme geben die Fehlermeldung richtig oder falsch. Dem Schuler werden Aufgaben vorgelegt, die sich aus mehreren Schritten und Prozessen zusammensetzen, wobei der Computer als Werkzeug bei der Suche nach einer $L\"{o}sung$ dient In guten $Probleml\"{o}sungsprogrammen$ verfolgt der Computer den $L\"{o}sungsansatz$ des $Sch\"{u}lers$ und analysiert seine Fehler. Wenn der Schuler bei der $\"{U}bung$ die richtige Antwort $ausw\"{a}hlt$, lauft das nachste Lernprogrammn des Computers. Die $\"{U}bungstyp$ kann auf dem Computer angereichert werden, indem $pr\"{a}zise$ und hilfreiche Kommentare $f\"{u}r$ jede $Auswahlm\"{o}glichkeit$ geboten werden.

• Journal of Microbiology and Biotechnology
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• v.29 no.9
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• pp.1460-1469
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• 2019

The extensive distribution of multidrug-resistant (MDR) methicillin-resistant Staphylococcus aureus (MRSA) poses a threat to healthcare worldwide. This study aimed to investigate the MDR and molecular patterns of MRSA isolates in children admitted to the two biggest tertiary care pediatric hospitals in northern and southern Vietnam. A total of 168 MRSA strains were collected to determine antibiotic susceptibility by minimum inhibitory concentration tests. Antibiotic-resistant genes, pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec (SCCmec) typing, and multilocus sequence typing were used for the molecular characterization of MRSA. Among the total strains, the MDR rate (51.8%) was significantly higher in the northern hospital than in the southern hospital (73% vs. 39%, p < 0.0001). The MDR-MRSA with the highest rates were "ciprofloxacin-erythromycin-gentamicintetracyclines" (35.6%), followed by "erythromycin-tetracycline-chloramphenicol" (24.1%), and "ciprofloxacin-erythromycin-gentamicin" (19.5%), showing an accumulative total of 79.3%. The most susceptible antibiotics were rifampicin (100%) and vancomycin (100%), followed by doxycycline (94.0%), meropenem (78.0%), and cefotaxime (75.0%). The SCCmecII strains showed greater resistance to gentamicin, ciprofloxacin, tetracycline, meropenem and cephalosporins compared with the other strains. The SCCmecII strains exhibited the highest rate in the tested genes (aacA/aphD: 55.2%, ermA/B/C: 89.7%, and tetK/M: 82.8%). ST5-SCCmecII was the predominant clone in the northern hospital, whereas SCCmecIVa was more pronounced in the southern hospital. In conclusion, our results raised concerns about the predominant MDR-MRSA strains in the pediatric hospitals in Vietnam. The north-south difference in the antibiotic resistance patterns and genetic structure of MRSA suggests different MRSA origins and various uses of antimicrobial agents between the two regions.

• Journal of Soil and Groundwater Environment
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• v.9 no.4
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• pp.8-14
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• 2004

To investigate the optimal operation factors in appling Soil Flushing Process, which use EDTA and boric acid composite as a flushing reagent, to the Cd(cadmium) and Cr(chromium) contaminated sites, Cd and Cr emediation rate was investigated at various experimental conditions; as EDTA concentration was increased from 0.001M to 0.1M, Cd remediation rate was increased from $73.2\%\;to\;98.5\%$; as boric acid concentration was increased from 0.001M to 0.1M, Cr remediation rate was increased from $59.2\%\;to\;99.0\%$. In the experiments using 0.005M of EDTA and 0.005M of boric acid composite as a flushing reagent at different pH regimes, Cd remediation rate was decreased from $92.4\%\;to;80.9\%$ as the pH was increased from 3 to 7. But Cr remediation rate was more effective ($70.4\%$) at pH 5 compared to the other pHs. Furthermore, at pH 5, Cd and Cr remediation rate was investigated at different molecular ratio of [EDTA]/[Boric aicd]; the optimized [EDTA]/[Boric acid] ratio was 0.01M/0.1M for Cd contaminated site and, as the concentration of boric acid was increased over 0.1M, Cr remediation rate was also increased. So the similar results were shown in the experiments using real contaminated soils which were sampled from a mining and a industrial area.

• Development and Reproduction
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• v.13 no.2
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• pp.89-95
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• 2009

p63 has been demonstrated to localize in stem cells and precursor cells of various epithelial tissues previously, but the localization of p63 throughout tooth formation, particularly during the enamel and root formation stages, remains to be adequately characterized. Therefore, in this study, we have demonstrated, via immunohistochemical methods, that p63 is ubiquitously expressed in the dental epithelium during tooth development. p63 was detected in the basal and suprabasal layers of the epithelia, including the skin, hair follicles, oral mucosa, and submandibular ducts. However, in the tooth region, all cells of the dental lamina, enamel organ, Hertwig's epithelial root sheath (HERS), and epithelial cell rests of Malassez (ERM) evidenced immunoreactivity for p63. These results indicate that p63 may perform different roles, other than stem cell maintenance, in tooth development.