• 한국식품저장유통학회지
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• 제24권3호
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• pp.381-386
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• 2017

Ergocalciferol은 vitamin D의 활성을 가지는 것으로 알려져 있다. 본 연구에서는 팽이(Flammulina velutipes), 느타리 (Pleurotus ostreatus), 애느타리(Pleurotus ostreatus), 새송이(Pleurotus eryngii), 양송이(Agaricus bisporus), 표고(Lentinula edodes), 목이(Auricularia auricula-judae) 등 7종의 버섯을 대상으로 자외선 조사에 따른 ergocalciferol(EC, 비타민 $D_2$) 함량의 증진 효과를 조사하였다. 자외선을 조사하지 않은 버섯의 ergocalciferol 함량은 극히 낮았으나 자외선 조사에 의해 ergocalciferol 함량이 크게 증가하였으며, 자외선 A, B, C 중 특히 자외선 B가 EC 함량 증진에 효과적이었다. 버섯에서 ergocalciferol의 생성은 자외선에 직접 노출되는 부위 즉 깊이 1 mm 이내의 버섯 표면에서만 일어나는 것으로 조사되었다. 따라서 세절하지 않은 생 버섯에 자외선을 조사할 경우 팽이 버섯과 같이 체적에 비해 표면적인 넓은 종류의 버섯에서 ergocalciferol 생성이 많았고, 건조 및 세절할 경우에는 EC 생성의 기질인 ergosterol의 함량이 높은 버섯에서 ergocalciferol 생성이 많았는데, 새송이, 표고, 양송이 등이 이에 속하였다.

• 대한치과의사협회지
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• 제14권4호
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• pp.359-366
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• 1976

• 한국식생활문화학회지
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• 제16권5호
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• pp.463-469
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• 2001

This study was to investigate the effect of ultraviolet rays, soaking, boiling and baking to ergocalciferol ($Vit.D_2$) and riboflavin($Vit.B_2$) contents of mushrooms, Lentinus edodes, Pleurotus ostreatus and Agaricus bisporus. The results were as follow: 1. Mushrooms were exposed to ultraviolet rays Lentinus edodes : $10J/cm^2$, Pleurotus ostratus : $2J/cm^2$ and Agaricus bisporus : $2J/cm^2$. 2. Before exposing to ultraviolet rays, the ergocalciferol contents of mushrooms were all $0{\mu}g/g$ dry base, but after exposing to it , those of Lentinus edodes, Pleurotus ostreatus and Agaricus bisporus were $222.50{\pm}5.30{\mu}g/g$ dry base, $150.90{\pm}6.60{\mu}g/g$ dry base and $23.98{\pm}1.20{\mu}g/g$ dry base, respectively 3. Before and after exposing to ultraviolet rays, the riboflavin contents of Lentinus edoes, Pleurotus ostreatus and Agaricus bisporus were $18.22{\pm}0.71{\mu}g/g$ dry base and $11.72{\pm}0.50{\mu}g/g$ dry base, $4.57{\pm}0.20{\mu}g/g$ dery base and $3.26{\pm}0.15{\mu}g/g$ dry base, and $37.42{\pm}1.20{\mu}g/g$ dry base and $27.33{\pm}2.10{\mu}g/g$ dry base, respectively. 4. The ergocalciferol contents of mushrooms according to boiling time were not significantly different but the riboflavin contents of them were decreased according to the increase of boiling time. 5. The ergocalciferol and riboflavin contents of mushrooms were decreased according to the increase of NaCl concentration and baking temperature. 6. The ergocalciferol content of Lentinus edodes after a short time soaking at $80^{\circ}C$ was higher than a long time soaking at $20^{\circ}C,\;40^{\circ}C$ and $60^{\circ}C$.

• Food Science and Biotechnology
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• 제14권6호
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• pp.828-831
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• 2005

The content of vitamin $D_2$, including its precursor ergosterol, was determined in some cultivated mushrooms to manufacture fortified Doenjang (Korean traditional soybean paste) with vitamin D by supplementation with mushroom. Ergosterol was the most abundant sterol in the mushrooms (50 to 140 mg/100 g dry weight) but the ergocalciferol portion made up only 0.065% (Pleurotus eryngii) to 2.5% (stipe part of Lentinus edodes, shiitake) of the total vitamin $D_2$ of each mushroom. Changes in these compounds in L. edodes caused by UV or solar irradiation were also evaluated. Ergocalciferol content in the pileus part of L. edodes went up to $424\;{\mu}g/100\;g$ dry weight and ergosterol levels reached 139.3 mg per 100 g dry weight at maximum levels. Ergocalciferol content increased about 50% when exposed to solar radiation and increased 377% with UV irradiation. These compounds level in Doenjang was enriched as much as supplied UV irradiated L. edodes powder to before fermentation, and the supplemented mushroom did not influence the palatability of Doenjang.

• 대한치과의사협회지
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• 제12권9호
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• pp.679-690
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• 1974

• 한국식품조리과학회지
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• 제13권2호
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• pp.173-178
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• 1997

HPLC를 이용하여 표고(동고, 화고), 팽이, 양송이, 목이, 석이, 운지, 영지버섯에 존재하는 비타민 D$_2$와 비타민 D$_3$의 양을 측정했다. HPLC는 external standard 방법을 이용하였다. 검화, 추출, 건조, 거름, 과정을 거친 시료 20 ${\mu}\ell$를 HPLC에 투입해 비타민 D$_2$와 비타민 D$_3$의 양을 측정했다 버섯들 중에 비타민 D$_2$와 비타민 D$_3$의 양이 가장 많은 것은 목이버섯(167.8$\mu\textrm{g}$/100g) 이었으며 가장 적은 것은 양송이로(7.2$\mu\textrm{g}$/100g)로 분석 됐다. 버섯에 존재하는 비타민 D의 함량이 연구자에 따라 많은 차이가 있는데 이는 버섯의 수확시기, 재배조건, 재배종자, 건조 조건에 기인한 것으로 보인다.

• 미생물학회지
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• 제49권2호
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• pp.191-194
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• 2013

아위느타리버섯(P. eryngii var. ferulae)의 비타민 $D_2$ (ergocalciferol) 함량 증강을 위하여 아위느타리버섯 자실체에 자외선(UV)-B파장(280-320 nm)을 조사하였다. 비타민 $D_2$ (ergocalciferol)의 증가된 함유량은 HPLC (Waters 1525, USA) 분석으로 확인하였다. 비타민 $D_2$ 함량은 3분($21.6KJ/m^2$) 처리시 $3.5{\mu}g/g$, 5분($36KJ/m^2$) 처리시 $6.02{\mu}g/g$으로 자외선을 조사하지 않은 대조군 $0.01{\mu}g/g$에 비교하여 높은 증가를 나타내었다. 이와 함께 큰느타리버섯(P. eryngii)은 대조군 $0.09{\mu}g/g$, 3분 처리시 $2.75{\mu}g/g$, 5분 처리시 $5.21{\mu}g/g$으로 나타났다. 표고버섯(L. edodes)은 대조군 $0.02{\mu}g/g$, 3분 처리시 $3.02{\mu}g/g$, 5분 처리시 $3.78{\mu}g/g$으로 나타났다. 느타리버섯(P. ostreatus)은 대조군 $0.19{\mu}g/g$, 3분 처리시 $9.63{\mu}g/g$, 5분 처리시 $11.60{\mu}g/g$으로 나타났다. 느타리버섯(P. ostreatus)의 비타민 $D_2$ 함량이 아위느타리버섯, 큰느타리버섯과 표고버섯 보다 높았으나 비타민 $D_2$ 함량 증가율은 아위느타리버섯이 월등히 높았다.

• 생명과학회지
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• 제32권5호
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• pp.368-374
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• 2022

본 연구에서는 표고버섯(Lentinula edodes)을 자외선 조사를 통한 ergocaciferol (비타민 D₂) 함량을 증진시킨 추출물을 이용하여 염증 및 알레르기 반응에 미치는 효과를 확인하였다. 표고버섯 추출물의 항염증 및 항알레르기 효능은 LPS로 활성화된 대식세포(RAW 264.7)와 PMA와 A23187에 의해 활성화된 비만세포(RBL-2H3)로부터 분비 또는 발현되는 TNF-α, IL-6, IL-1β, IL-4와 같은 cytokine과 histamine 분비량을 측정하여 관찰하였다. LPS에 의해 활성화된 대식세포에서 자외선 조사 표고버섯 추출물 처리에 의해 pro-inflammatory cytokine인 TNF-α와 IL-6의 분비량을 ELISA 방법으로 측정하였을 때 현저히 감소함을 확인하였고 mRNA 발현 또한 감소됨을 확인하였다. 비만세포에 자외선 조사 표고버섯 추출물과 PMA, A23187을 함께 처리한 경우 비만세포의 탈과립에 의해 분비되는 histamine의 양이 유의적으로 감소함을 확인하였고 IL-4의 발현양 또한 감소함을 확인할 수 있었다. 이상의 결과는 자외선 조사에 의해 비타민 D₂ 함량을 증진시킨 표고버섯 추출물이 염증과 알레르기 반응의 cytokine의 발현을 저해하는 것으로 보아 염증 및 알레르기 질환의 예방과 치료에 효과적으로 이용될 수 있을 것으로 사료된다.

• Clinical and Experimental Pediatrics
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• 제54권2호
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• pp.51-54
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• 2011

Vitamin D is present in two forms, ergocalciferol (vitamin $D_2$) produced by plants and cholecalciferol (vitamin $D_3$) produced by animal tissues or by the action of ultraviolet light on 7-dehydrocholesterol in human skin. Both forms of vitamin D are biologically inactive pro-hormones that must undergo sequential hydroxylations in the liver and the kidney before they can bind to and activate the vitamin D receptor. The hormonally active form of vitamin D, 1,25-dihydroxyvitamin D3 $[1,25(OH)_2D]$, plays an essential role in calcium and phosphate metabolism, bone growth, and cellular differentiation. Renal synthesis of $1,25(OH)_2D$ from its endogenous precursor, 25-hydroxyvitamin D (25OHD), is the rate-limiting and is catalyzed by the $1{\alpha}$-hydroxylase. Vitamin D dependent rickets type I (VDDR-I), also referred to as vitamin D $1{\alpha}$-hydroxylase deficiency or pseudovitamin D deficiency rickets, is an autosomal recessive disorder characterized clinically by hypotonia, muscle weakness, growth failure, hypocalcemic seizures in early infancy, and radiographic findings of rickets. Characteristic laboratory features are hypocalcemia, increased serum concentrations of parathyroid hormone (PTH), and low or undetectable serum concentrations of $1,25(OH)_2D$ despite normal or increased concentrations of 25OHD. Recent advances have showed in the cloning of the human $1{\alpha}$-hydroxylase and revealed mutations in its gene that cause VDDR-I. This review presents the biology of vitamin D, and $1{\alpha}$-hydroxylase mutations with clinical findings.

• 대한소아치과학회지
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• 제11권1호
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• pp.131-143
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• 1984

150 rats weighting about 150gm were devided into control group of 80 and experimental group of 70. Control group was subdivided into the irradiated vitamin D injection group and X-ray irradiated group. Experimental group was given 2.0mg ergocalciferol by four intramuscular injection prior to X-ray irradiation with single 800 rads and 1,500 rads respectively. Experimental animals from each group was sacrificed after 1, 3, 7, 14, and 28 days and their incisors were investigated by histopathological examination. The results were as follows; 1. In the irradiated groups, it showed dentin hypoplasia and formation of dentinoid substance caused by degeneration of odontoblast at the early stage. Especially, 1,500 rads group which was severely effected showed formation of osteoid dentin at the apical portion and severe injuries of dental papilla at the first week. 2. In the vitamin D2 administration group, it showed thinned dentin layer at the early stage but, taking time, predentin and dentin layer was thickened. At the fourth week, dentin was chiefly composed of interglobular dentin, especially in the lingual portion. 3. Using in combination of overdose vitamin D2 administration and X-ray irradiation, it effected severely odontoblast, undifferentiated mesenchymal cells around tooth germ and pulp tissue. At the early stage, dentin layer was thinned but, taking time, it was thickened and composed of interglobular dentin caused by calcification of predentin layer. 4. In 800 rads irradiation after the overdose vitamin D2 administration, it showed formation of osteoid dentin in the lingual portion at the first week. In the 1,500 rads irradiation after the overdose vitamin D2 administration, it showed formation of osteoid dentin and degeneration of ameloblast in both buccal and lingual portion at the first week, and enamel hypoplasia caused by edema and loss of polarity of ameloblasts at the second week. 5. By the entire experiment, the overdose vitamin D2 administration and X-ray irradiation effected severely odontoblasts, undifferentiated mesenchymal cells of dental papilla, and primitive cells of tooth germ among the dental tissue. Especially using combination of overdose vitamin D2 administration and X-ray irradiation also effected ameloblasts, resulting in enamel hypoplasia.