• Industrial Engineering and Management Systems
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• 제12권3호
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• pp.207-223
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• 2013

Revenue management (RM) has been widely used to model products characterized as perishable. Classical RM model assumed that price is the sole factor in the model. Thus price adjustment becomes a crucial and costly factor in business. In this paper, an optimal pricing model is developed based on minimization of soft customer cost, one kind of price adjustment cost and is solved by Lagrange multiplier method. It is formed by expected discounted revenue/bid price integrating quantity-based RM and pricing-based RM. Quantity-based RM consists of two capacity models, namely, booking limit and overbooking. Booking limit, built by assuming uncertain customer arrival, decides the optimal capacity allocation for two market segments. Overbooking determines the level of accepted order exceeding capacity to anticipate probability of cancellation. Furthermore, pricing-based RM models occupancy/demand rate influenced by internal and competitor price changes. In this paper, a mathematical model based on game theoretic approach is developed for two conditions of deterministic and stochastic demand. Based on the equilibrium point, the best strategy for both hotels can be determined.

• 한국구조물진단유지관리공학회 논문집
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• 제8권4호
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• pp.257-264
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• 2004

공동주택 수익모델의 가치를 형성하는 요소 중 영향도가 큰 물리적 요소는 주택의 평형변화로서 이를 중심으로 리모델링의 대안을 제안한다. 이에 따라 본 연구에서는 공동주택의 가치형성요인과 공간구성기법을 이용한 큰 평형의 세대를 작은 평형의 세대로 분할하는 모델과 작은 평형의 세대를 통합하여 큰 평형의 세대로 변환하는 모델의 수익모델을 설정하여 경제성은 '보통'이상 '양호'한 것으로 나타났으며, 경제성 평가값은 할인율이 증가함에 따라 감소하며 리모델링의 경제성이 우수해지는 것으로 나타남을 알 수 있다.

• Bulletin of the Korean Chemical Society
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• 제27권2호
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• pp.224-230
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• 2006

Escherichia coli transcription termination factor Rho catalyzes the unwinding of RNA/DNA duplex in reactions that are coupled to ATP binding and hydrolysis. We report here the kinetic mechanism of presteady state ATP binding and hydrolysis by the Rho-RNA complex. Presteady state chemical quenched-flow technique under multiple turnover condition was used to probe the kinetics of ATP binding and hydrolysis by the Rho-RNA complex. The quenched-flow presteady state kinetics of ATP hydrolysis studies show that three ATPs are bound to the Rho-RNA complex with a rate of $4.4\;{\times}\;10^5M^{-1}s^{-1}$, which are subsequently hydrolyzed at a rate of $88s^{-1}$ and released during the initiation process. Global fit of the presteady state ATP hydrolysis kinetic data suggests that a rapid-equilibrium binding of ATP to Rho-RNA complex occurs prior to the first turnover and the chemistry step is not reversible. The initial burst of three ATPs hydrolysis was proposed to be involved in the initialization step that accompanies proper complex formation of Rho-RNA. Based on these results a kinetic model for initiation process for Rho-RNA complex was proposed relating the mechanism of ATP binding and hydrolysis by Rho to the structural transitions of Rho-RNA complex to reach the steady state phase, which is implicated during translocation along the RNA.

• STI Policy Review
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• 제8권1호
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• pp.1-22
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• 2017

There has been a pronounced increase in research and development (R&D) expenditure in Singapore over the last two decades, with government spending accounting for a sizeable share. This increase has been spurred by public policy emphasis on research and innovation as engines of economic growth. This paper analyses the impact of R&D on economic performance in Singapore from 1978 to 2012 through the use of time series analysis. The Cobb-Douglas based analysis shows a long-run equilibrium relationship between Total Factor Productivity (TFP) and R&D investments. We found that the short-run productivity of R&D in Singapore is comparable to smaller advanced economies in the Organisation for Economic Co-operation and Development (OECD). However, in terms of long-run R&D productivity, Singapore lags slightly behind the smaller OECD nations and far behind the G7 countries. This suggests leakage of value capture and low absorptive capacity in local firms. Possibility of productivity improvements induced by policy changes in the 1990s was considered, but no evidence of significant structural breaks was found. Lastly, Granger causality analysis reveals that public sector R&D augments private sector R&D capital, thus playing an important role in generating externalities and spillover effects. Policy implications and lessons for other middle-income countries are discussed.

• 한국지반공학회논문집
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• 제24권3호
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• pp.5-11
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• 2008

이 논문에서는 사면 안정 해석시 경사 절편법을 이용하여 안전율을 구하며 유전자 알고리즘방법을 이용하여 한계파괴면을 결정하는 이론을 제안하였다. 해석방법에서는 한계 전단 파괴면과 안전율을 찾고 비선형 평형 방정식의 해를 구하기 위해 구속 최적화 문제로 간주하여 해석을 수행하였다. 유전자 알고리즘 방법의 효율성을 검증하기 위하여 예제를 논문에 포함하였다. 유전자 알고리즘 방법에 의하여 도출된 사면 안정 해석결과는 기존방법에 비하여 우수한 것으로 판명되었다.

• 한국지반공학회논문집
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• 제31권1호
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• pp.47-52
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• 2015

사면안정분야의 해석과 설계에는 한계평형법(LEM)과 전단강도 감소기법(SSR)이 주로 사용된다. 이 두 방법은 2차원과 3차원 해석을 모두 수행할 수 있지만 많은 연구자들에 의해 전단강도 감소기법이 해석 변수에 대해 더욱 민감하게 반응하고 합리적인 결과를 산출한다고 알려져있다. 그러나 전단강도 감소기법의 모델링의 복잡함과 해석시간의 증가 등의 이유로 현계평형법이 여전히 일반적으로 사용되고 있다. 본 연구에서는 FLAC 3D를 사용하여 전단강도 감소기법을 통한 돌출된 암반사면의 3차원 효과에 대해 연구하였다. 수치해석 변수는 사면의 경사, 높이, 지반강도, 사면의 돌출길이이며 연구 결과 사면의 안전율은 암반의 강도보다 사면의 형상에 더 영향을 받는 것으로 나타났다.

• BMB Reports
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• 제43권3호
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• pp.176-181
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• 2010

The primary sigma factor ($\sigma^{A}$) of Staphylococcus aureus, a potential drug target, was little investigated at the structural level. Using an N-terminal histidine-tagged $\sigma^{A}$ (His-$\sigma^{A}$), here we have demonstrated that it exits as a monomer in solution, possesses multiple domains, harbors primarily $\alpha$-helix and efficiently binds to a S. aureus promoter DNA in the presence of core RNA polymerase. While both N- and C-terminal ends of His-$\sigma^{A}$ are flexible in nature, two Trp residues in its DNA binding region are buried. Upon increasing the incubation temperature from 25$^{\circ}$ to 40$^{\circ}C$, $\sim$60% of the input His-$\sigma^{A}$ was cleaved by thermolysin. Aggregation of His-$\sigma^{A}$ was also initiated rapidly at 45$^{\circ}C$. From the equilibrium unfolding experiment, the Gibbs free energy of stabilization of His-$\sigma^{A}$ was estimated to be +0.70 kcal $mol^{-1}$. The data together suggest that primary sigma factor of S. aureus is an unstable protein. Core RNA polymerase however stabilized $\sigma^{A}$ appreciably.

• Nuclear Engineering and Technology
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• 제47권1호
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• pp.47-58
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• 2015

Four recycling scenarios involving pyroprocessing of spent fuel (SF) have been investigated for a 600-MWe transmutation sodium-cooled fast reactor (SFR), KALIMER. Performance evaluation was done with code system REBUS connected with TRANSX and TWODANT. Scenario Number 1 is the pyroprocessing of Canada deuterium uranium (CANDU) SF. Because the recycling of CANDU SF does not have any safety problems, the CANDU-Pyro-SFR system will be possible if the pyroprocessing capacity is large enough. Scenario Number 2 is a feasibility test of feed SF from a pressurized water reactor PWR. Thefsensitivity of cooling time before prior to pyro-processing was studied. As the cooling time sensitivity of cooling time before prior to pyro-processing was studied. As the cooling time increases, excess reactivity at the beginning of the equilibrium cycle (BOEC) decreases, thereby creating advantageous reactivity control and improving the transmutation performance of minor actinides. Scenario Number 3 is a case study for various levels of recovery factors of transuranic isotopes (TRUs). If long-lived fission products can be separated during pyroprocessing, the waste that is not recovered is classified as low- and intermediate-level waste, and it is sufficient to be disposed of in an underground site due to very low-heat-generation rate when the waste cooling time becomes >300 years at a TRU recovery factor of 99.9%. Scenario Number 4 is a case study for the recovery factor of rare earth (RE) isotopes. The RE isotope recovery factor should be lowered to ${\leq}20%$ in order to make sodium void reactivity less than <7$, which is the design limit of a metal fuel.

• 자원리싸이클링
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• 제14권2호
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• pp.33-42
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• 2005

Nd와 Sm이 혼합된 염산용액에서 PC88A에 의한 두 금속의 분리에 대한 실험을 수행하였다. 또한 PC88A의 비누화가 두 금속의 추출 및 분리에 미치는 영향을 조사하였다. 본 연구에서 수행한 실험조건에서 Sm의 분배계수가 Nd의 분배계수보다 크며, 분리인자는 수상의 pH에 따라 증가하였다. 비누화 PC88A로 추출하는 경우 PC88A로 추출하는 경우에 비해 분배계수와 분리인자 모두 증가하였다. PC88A와 비누화 PC88A에 의한 추출시 초기추출조건으로부터 Nd와 Sm의 분배계수와 분리인자를 예측할 수 잇는 모델을 개발하였다. 본 연구에서 개발된 모델을 초기추출조건에 적용하여 예측한 Nd와 Sm의 분배계수는 실험으로 측정한 값과 서로 잘 일치하였다.

• Biomolecules & Therapeutics
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• 제19권4호
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• pp.419-424
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• 2011

Galla Rhois and its components are known to possess anti-infl ammatory properties. In the present study, we prepared equilibrium mixture of ethyl m-digallate and ethyl p-digallate isomers (EDG) from Galla Rhois and examined its effect on nitric oxide (NO) production in murine macrophage cell line. Treatment of RAW264.7 macrophages with EDG signifi cantly inhibited NO production and inducible nitric oxide synthase (iNOS) expression stimulated by LPS, as assessed by Western blot and quantitative RT-PCR analyses. We also demonstrated that EDG treatment led to an increase in heme oxygenase-1 (HO-1) mRNA and protein expression. EDG treatment also enhanced expression level of nuclear factor-erythroid 2-related factor 2 (Nrf2) in nucleus, which is critical for transcriptional induction of HO-1. Treatment with SnPP (tin protoporphyrin IX), a selective HO-1 inhibitor, reversed EDG-mediated inhibition of nitrite production, suggesting that HO-1 plays an important role in the suppression of NO production by EDG. Taken together, these results indicate that EDG isolated from Galla Rhois suppresses LPS-stimulated NO production in RAW 264.7 macrophages via HO-1 induction.