• Title/Summary/Keyword: enzymatic treatment

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Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production

  • Kim, Hak-Gon;Song, Hyun-Jin;Jeong, Mi-Jin;Sim, Seon-Jeong;Park, Dong-Jin;Yang, Jae-Kyung;Yoo, Seok-Bong;Yeo, Jin-Ki;Karigar, Chandrakant S.;Choi, Myung-Suk
    • Journal of Forest and Environmental Science
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    • v.27 no.3
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    • pp.143-149
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    • 2011
  • The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

Strength Restoration of DP Finished Cotton Fabric by Enzymatic Treatment (효소처리에 의한 DP 가공 면직물의 강도회복)

  • 김주혜;김수연;최은경;이현경
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.04a
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    • pp.429-430
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    • 2003
  • Durable Finish(DP) 가공은 면직물이나 의복의 구김 방지성을 주기 위해 널리 사용되어진다 이는 비결정부분에 위치한 셀룰로오즈 분자의 체인 사이에 수지가 가교결합을 형성하여(Figure 1) 네트워크 형태의 거대 분자구조를 만들고 체인간의 움직임을 억제하여 섬유가 뻣뻣하게 된다. 이로 인해 면섬유의 인장강도, 인열강도 등 물리적 강도가 심각하게 저하된다. 본 논문에서는 현재 durable finishing에 가장 많이 사용하는 Dimethylol Dihydroxy Ethylene Urea(DMDHEU)를 이용한 DP 가공 면직물의 강도저하를 관찰하고 선택된 효소처리가 강도회복에 미치는 영향을 연구하였다. (중략)

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Antioxidative Enzymatic Activity of Saururus Chinensis Baill & Houttuynia cordata Thunb in the liver of rats treated with CCI4

  • Park, Sun-Yi;Jung , Hae-Jin;Ha , Bae-Jin
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.319.3-320
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    • 2002
  • Saururus chinensis Baill (Saururaceae) is a perennial plant that has been used in the treatment of edema. jaundice and gonorrhea in Korean folk medicine. Houttuynin sodium bisulphate (HSB), alpha hydroxyl-capryl-ethyl-sodium-sulphonate. is a product formed by reacting sodium bisulphate with houttuynin. which is obtained from a medicinal herb Houttuynia cordata Thunb. (omitted)

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Enzymatic and/or chemical dosages coupled with the clarification of the sample applied to the screening evaluation of heat load in commercial milks

  • Humbert, Gerard
    • 한국유가공학회:학술대회논문집
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    • 2005.10a
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    • pp.11-19
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    • 2005
  • Heat treatment is essential for hygienic safety and for extending shelf-life of milk. Heating of milk affects principally its physicochemical, nutritional and organoleptic properties. The most important changes are the decrease in whey protein solubility and the decrease in stability of casein micelles. Maillard reactions are also important and undesirable consequences.

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Apoptosis by Direct Current Treatment in Tumor Cells and Tumor Tissues

  • Kim, H. B.;S. B. Sim;S. Ahn
    • Proceedings of the Korean Biophysical Society Conference
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    • 2003.06a
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    • pp.48-48
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    • 2003
  • Electric field induces cell fusion, electroporation on biological cells, including apoptosis. Apoptosis is expressed in a series of natural enzymatic reactions for the natural elimination of unhealthy, genetically damaged, or otherwise aberrant cels that are not needed or not advantageous to the well-being of the organism. Its markers involve cell shringkage, activation of intracellular caspase proteases, externalization of phosphatidylserine at the plasma membrane, and fragmentation of DNA.

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Texture Characteristics of Horse Meat for the Elderly Based on the Enzyme Treatment

  • Kim, Dah-Sol;Joo, Nami
    • Food Science of Animal Resources
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    • v.40 no.1
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    • pp.74-86
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    • 2020
  • Horse meat is nutritionally adequate to the elderly, but it has a comparatively hard texture in contrast to most of the food. In practice, the meat intake in the elderly is generally bated because the relatively difficult texture of the meat can diminish mastication. Thus, strategies are being developed to produce meat products remanding detracted mastication exertion and possibly exalt ingestion and nutritional stand, in the elderly. Hence, the effects of enzymes on textural characteristics of horse meat were studied, because they have well-known favorable efficacy on the meat tenderness by causing important demotion of the myo-fibrillar protein and collagen. Four treatments namely, papain, bromelin, pepsin, and pancreatin, alongside one control were invoked to the horse meat. Their effects on the texture parameters were determined. All the above enzymatic treatments significantly reduced hardness and resilience (p<0.001). These results present opportunities to produce essential fatty acids fortified horse meat with soft texture and satisfied technological characteristics. The intake of the essential fatty acids intensified horse meat could aid the elderly to get their aimed essential fatty acid demands. Results also suggest that horse meat tenderized through enzymatic processing stand for auspicious options for the comprehension of texture-revised diets in the elderly population.

Enzymatic Release of Ferulic Acid from Ipomoea batatas L. (Sweet Potato) Stem

  • Min, Ji-Yun;Kang, Seung-Mi;Park, Dong-Jin;Kim, Yong-Duck;Jung, Ha-Na;Yang, Jae-Kyung;Seo, Won-Teak;Kim, Seon-Won;Karigar, Chandrakant S.;Choi, Myung-Suk
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.4
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    • pp.372-376
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    • 2006
  • Ferulic acid is a phenolic compound that serves as a major biosynthetic precursor of vanillin in higher plants. We investigated the ability of the 3 commercial enzymes - Ultraflo L, Viscozyme L, and ${\alpha}-Amylase$ - to induce the release ferulic acid from the Ipomoea batatas L. (sweet potato) stem. The rate of release for ferulic acid was optimal when Ultraflo L (1.0%) was used compared with the other enzymes, whereas Viscozyme L was most effective for the release of vanillic acid and vanillin. Thus, these enzymes may be useful for the large-scale production of ferulic acid and other phenolic compounds from sweet potato stem.

Bacterial Surface Display of Levansucrase of Zymomonas mobilis Using Bacillus Subtilis Spore Display System (고초균 포자를 이용한 Zymomonas mobilis 유래의 levansucrase 표면 발현)

  • Kim, June-Hyung;Choi, Soo-Keun;Jung, Heung-Chae;Pan, Jae-Gu;Kim, Byung-Gee
    • KSBB Journal
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    • v.26 no.3
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    • pp.243-247
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    • 2011
  • Using Bacillus subtilis spore display system, with cotG as an anchoring motif, levansucrase from Zymomonas mobilis, was displayed on the outer surface of Bacillus subtilis spore. Flow cytometry of DB104 (pSDJH-cotG-levU) spore, proved the surface localization of CotG-LevU fusion protein on the spore compared to that of DB104. Enzymatic activity of DB104 (pSDJH-cotG-levU) spore showed more than 1.5 times higher levansucrase specific activity compared to that of the host spore, which is a remarkable increase of enzymatic activity considering the existence of sacA (sucrase) and sacB (levansucrase) in the Bacillus subtilis chromosome. The spore integrity, revealed by sporulation frequency test after heat and lysozyme treatment of spore, did not changed at all in spite of the CotG-LevU fusion protein incorporation into the spore coat layer during spore formation process. These data prove again that Bacillus subtilis spore could be considered as good live immobilization vehicle for efficient bioconversion process.

Enzymological Evaluation of Bowel Inflammation Inhibitory Activity and Intestinal Microbial Flora Improvement by Enzymatic Hydrolysate of Schizandrae Fructus (오미자 효소가수분해물에 의한 장내 세균총 개선 및 장관 염증 억제 효과의 효소학적 평가)

  • Ryu, Il-Hwan;Kwon, Tae-Oh;Lee, Kang-Soo;Yun, Yong-Gab
    • Korean Journal of Pharmacognosy
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    • v.38 no.4
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    • pp.363-371
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    • 2007
  • Schizandrae Fruits has been used as a traditional Oriental medicine for treatment of many stress-induced diseases. In the present study, we investigated inhibitory activity of enzymatic hydrolysate of Schizandrae fructus (SC-EX) in growth of tested intestinal microorganism and activity of bowel inflammation related enzyme. SC-EX was added to the proteose peptone-yeast extract-fildes (PYF) media to investigation the effect on the growth of type culture of intestinal microorganism. The growth of lactic acid bacteria such as Bifidobacterium species and Lactobacillus species was accelerated by more than 3% concentration of SC-EX. But, growth of harmfulness bacteria such as E.coli, Clostridium sp. Staphylococcus sp. Streptococcus sp. was inhibited by more than 3% concentration of SC-EX. Also, SC-EX was exhibited a concentration-dependent inhibitory activity of the bowel inflammation related enzymes. The SC-EX was showed 76% and 92% inhibitory activity of 5-lipoxygenase and cyclooygenase at 5% additional concentration respectively. Our results indicated that SC-EX may possess improvement effect on the intestinal flora and Anti-inflammatory effect on the bowel.