• Title/Summary/Keyword: environmental sample

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Study on the Isolation of Patulin-Producing Penicillium sp. from Natural Sources (자연계로부터 Patulin 생성 Penicillium sp.의 분야에 관한 연구)

  • 김동술;정덕화;김성영;정선희;강성조;전향숙
    • Journal of Environmental Health Sciences
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    • v.19 no.3
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    • pp.41-45
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    • 1993
  • To study the prevention of mycotoxin contamination, a large number of sample sources were collected, and Penicilliurn sp. were isolated. TLC and HPLC methods were applied to confirm the patulin producing abilities of isolated strains. From 321 sample sources such as fruit (46), rice (31), corn (19), barley (22) and soil (38), 203 strains of Penicilliurn sp. were isolated. By the result of TLC, 21 strains were assumed to be patulin producing strains, but as a result of HPLC, only 17 strains produced patulin. Among them, E-219 strain showed highest patulin level as 0.12 mg/ml broth.

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Improvement of Environmental Management in Small and Medium-Sized Firms through the Implementation of Environmental Management System (환경경영시스템의 도입에 따른 중소기업의 환경경영 개선)

  • 이근상;정혜철
    • Journal of Korean Society for Quality Management
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    • v.32 no.2
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    • pp.1-14
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    • 2004
  • As environmental issues have been become critical concerns of business, many domestic small and medium enterprises(SME's) are rapidly accepting environmental management systems(EMS) that confirm ISO 14001 guidelines. The purpose of this paper is to present the results of an empirical study carried out on a sample of certified SME's with the aim of identifying obstacles encountered in adopting ISO 14001 standards and the impact of environmental systems on corporate performance after implementation of the ISO 14001 registration process. Data from this study show that the vast majority of respondents reported environmental improvements related to the implementation of an ISO 14001 EMS, especially in the area of employees' participation of the environmental management and responsibilities for reducing negative impacts, energy and resource conservation, materials reuse, and raising top management commitment. The greatest obstacles for implementing the ISO 14001 standard are lack of cooperation and a scarcity of resources such as personnel, money, and time. Results of this study serve as a reference point understanding the problems in the implementation of ISO 14001 in other small and medium firms.

Devleopment and Verification of Questionnaire for Measurement of Environmental Attitude (환경의식의 측정을 위한 설문지 개발과 검증)

  • 우형택;엄붕훈;문연화
    • Journal of Environmental Science International
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    • v.8 no.5
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    • pp.559-568
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    • 1999
  • This study was conducted to develop the questionnaire for the measurement of environmental attitudes and test its reliability and validity. Development and valiadation of the 46 survey items that measure environmental attitudes were described. The total of 3 surveys were conducted and the total of 510 persons including diverse sample groups were questionnaired. The SAS $PC^+$ package program was used in both the reliability analysis and the validity analysis of surveyed data. Results revealed the very high reliability and the high validity of the questionnaire. The reliability of the finalized questionnaire was increased to the level of Cronbach-$\alpha$ 0.92 after the repeated reliability analyses and revisions of the firstly and secondly designed questionnaires. The validity of our questionnaire was sufficiently affirmed through the use and aplication of factor analysis and known-groups technique.

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Classification of Ambient Particulate Samples Using Cluster Analysis and Disjoint Principal Component Analysis (군집분석법과 분산주성분분석법을 이용한 대기분진시료의 분류)

  • 유상준;김동술
    • Journal of Korean Society for Atmospheric Environment
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    • v.13 no.1
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    • pp.51-63
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    • 1997
  • Total suspended particulate matters in the ambient air were analyzed for eight chemical elements (Ca, Co, Cu, Fe, Mn, Pb, Si, and Zn) using an x-ray fluorescence spectrometry (XRF) at the Kyung Hee University - Suwon Campus during 1989 to 1994. To use these data as basis for source identification study, membership of each sample was selected to represent one of the well defined sample groups. The data sets consisting of 83 objects and 8 variables were initially separated into two groups, fine (d$_{p}$<3.3 ${\mu}{\textrm}{m}$) and coarse particle groups (d$_{p}$>3.3 ${\mu}{\textrm}{m}$). A hierarchical clustering method was examined to obtain possible member of homogeneous sample classes for each of the two groups by transforming raw data and by applying various distances. A disjoint principal component analysis was then used to define homogeneous sample classes after deleting outliers. Each of five homogeneous sample classes was determined for the fine and the coarse particle group, respectively. The data were properly classified via an application of logarithmic transformation and Euclidean distance concept. After determining homogeneous classes, correlation coefficients among eight chemical variables within all the homogeneous classes for calculated and meteorological variables (temperature. relative humidity, wind speed, wind direction, and precipitation) were examined as well to intensively interpret environmental factors influencing the characteristics of each class for each group. According to our analysis, we found that each class had its own distinct seasonal pattern that was affected most sensitively by wind direction.ion.

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Spatial Heterogeneity of Bacteria: Evidence from Hot Composts by Culture-independent Analysis

  • Guo, Yan;Zhang, Jinliang;Deng, Changyan;Zhu, Nengwu
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.7
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    • pp.1045-1054
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    • 2012
  • The phylogenetic diversity of the bacteria in hot composting samples collected from three spatial locations was investigated by molecular tools in order to determine the influence of gradient effect on bacterial communities during the thermophilic phase of composting swine manure with rice straw. Total microbial DNA was extracted and bacterial near full-length 16S rRNA genes were subsequently amplified, cloned, restriction fragment length polymorphism-screened and sequenced. The superstratum sample had the highest microbial diversity among the three samples which was possibly related to the surrounding conditions of the sample resulting from the location. The results showed that the sequences related to Bacillus sp. were most common in the composts. In superstratum sample, 45 clones (33%) and 36 clones (27%) were affiliated with the Bacillus sp. and Clostridium sp., respectively; 74 clones (58%) were affiliated with the Clostridium sp. in the middle-level sample; 52 clones (40%) and 29 clones (23%) were affiliated with the Clostridium sp. and Bacillus sp. in substrate sample, respectively. It indicated that the microbial diversity and community in the samples were different for each sampling site, and different locations of the same pile often contained distinct and different microbial communities.

Optimized Design of Dioxin Analysis for Water Sample

  • Choi, Jaewon;Lee, Jaehee;Kim, Kyoungsim;Kim, Sunheong;Bae, Kyunghee
    • Journal of Korean Society on Water Environment
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    • v.21 no.3
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    • pp.219-229
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    • 2005
  • The analytical methods for dioxins in water sample from wastewater to tap water were reviewed. For extraction method, liquid-liquid extraction (LLE) has been widely used, however, this process needs too much time and man power. New approach including solid phase extraction (SPE) is now applicable to large volume of water sample with high extraction efficiency. Column clean up in classical analytical methods were very complex and time consuming procedures during decade. Modifications were tried to decrease solvent and reagents volume. Moreover, use of column connection method has been demonstrated in the environmental matrices. Instrumental configurations also have been improved, in which GC/MS/MS with large volume injection approach can analyze picogram levels. Absolute sensitivities of HRMS increased compared to old versions of double focusing sector type mass spectrometers. Based on these analytical evolutions during last 10 years, we tried to optimize the analytical method for dioxins in water sample from sample extraction to instrumental analysis.

Sampling and Analytical Method for Linear Carbonates using GC/FID (GC/FID를 이용한 사슬형 카보네이트 3종의 측정·분석방법)

  • Miyeon Jang;Gwangyong Yi
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.33 no.4
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    • pp.455-463
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    • 2023
  • Objectives: The aim of this study was to develop an air sampling and analytical method for estimating worker exposure to linear carbonate solvents and to evaluate the method. Methods: The target substances were three linear carbonates: DMC, DEC, EMC. GC/FID was used for sample analysis. Laboratory experiments were conducted to determine desorption solvents and sample capacity, and to evaluate storage stability, accuracy, and precision. Results: Coconut Shell Charcoal (CSC, 100/50 mg) was used as the air sampling media, and a desorption solvent of 5% methanol/95% dichloromethane was selected. Recommended sampling capacities were 1~11 ℓ for DMC, 1~18 ℓ for DEC, and 1~24 ℓ for EMC. The stability of three linear carbonates was demonstrated over 30 days in a refrigerator (4℃). Detection limits were determined as follows: DMC 0.26 ㎍/sample, DEC 0.24 ㎍/sample, and EMC 0.25 ㎍/sample. The total coefficient of variation was calculated as DMC 0.064, DEC 0.079, and EMC 0.07. Conclusions: This sampling and analysis method is suitable for estimating personal exposure to linear carbonates in the workplace.

Study on Reutilization with Aerobic Microbes of Organic Food Waste Leachates (호기성 미생물을 이용한 음폐수의 처리 및 자원화에 관한 연구)

  • Kang, Bo-Mi;Hwang, Hyeon-Uk;Kim, Ji-Hoon;Yang, Yong-Woon;Kim, Young-Ju
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.1
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    • pp.54-59
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    • 2011
  • This test established the bioavailability and sample input by mixing the maintaining the microbial machine parts and food waste leachates in weight of 2:1 as advanced experiment, maintaining the constant temperature, agitating and observing its weight and property change for 60 hours. And, I injected daily the established microbial machine parts and food waste leachates rate, maintained the temperature in the reactor with $55{\sim}65^{\circ}C$, and agitated with constant speed. I studied the recycling possibility of food waste leachates by extracting the sample after 24 hours, verifying its characteristics, and repeating the food waste leachates input and sample extraction for about 40 days. Considering all about the results of this study, I saw that 87.32% of food waste leachates was reduced, and the solid of bluebug or food included in the food waste leachates was decomposed within 24 hrs. pH for 43 days after 9 days of stabilization period was maintained from 3.7~3.9 and the ignition loss from 88.67~87.3%, and the quantity of organic matter from 77.6~80.88%. With the similar result daily maintained, it is considered to progress more the minimization by inputting the future food waste leachates. C/N rate satisfies the less than 25 that is the composting basis within 8 days, maintaining between 13~15, with 2% of salt not exceeded, it is able to recycle as the compost of food waste leachates as based on the composting with no extracted heavy metal content.

Spatial Estimation of Forest Species Diversity Index by Applying Spatial Interpolation Method - Based on 1st Forest Health Management data- (공간보간법 적용을 통한 산림 종다양성지수의 공간적 추정 - 제1차 산림의 건강·활력도 조사 자료를 이용하여 -)

  • Lee, Jun-Hee;Ryu, Ji-Eun;Choi, Yu-Young;Chung, Hye-In;Jeon, Seong-Woo;Lim, Jong-Hwan;Choi, Hyung-Soon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.22 no.4
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    • pp.1-14
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    • 2019
  • The 1st Forest Health Management survey was conducted to examine the health of the forests in Korea. However, in order to understand the health of the forests, which account for 63.7% of the total land area in South Korea, it is necessary to comprehensively spatialize the results of the survey beyond the sampling points. In this regard, out of the sample points of the 1st Forest Health Management survey in Gyeongbuk area, 78 spots were selected. For these spots, the species diversity index was selected from the survey sections, and the spatial interpolation method was applied. Inverse distance weighted (IDW), Ordinary Kriging and Ordinary Cokriging were applied as spatial interpolation methods. Ordinary Cokriging was performed by selecting vegetation indices which are highly correlated with species diversity index as a secondary variable. The vegetation indices - Normalized Differential Vegetation Index(NDVI), Leaf Area Index(LAI), Sample Ratio(SR) and Soil Adjusted Vegetation Index(SAVI) - were extracted from Landsat 8 OLI. Verification was performed by the spatial interpolation method with Mean Error(ME) and Root Mean Square Error(RMSE). As a result, Ordinary Cokriging using SR showed the most accurate result with ME value of 0.0000218 and RMSE value of 0.63983. Ordinary Cokriging using SR was proven to be more accurate than Ordinary Kriging, IDW, using one variable. This indicates that the spatial interpolation method using the vegetation indices is more suitable for spatialization of the biodiversity index sample points of 1st Forest Health Management survey.