• Food Science of Animal Resources
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• v.33 no.2
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• pp.198-204
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• 2013

This study aimed to apply the Parallux system to detect (fluoro)quinone antibiotics residues in raw bovine milk. The immunogen enabled the generation of a specific antiserum with a titer of 1/40,000. The $Parallax^{TM}$ kit using the antibody displayed $IC_{50}$ value of 10 to 150 ppb for (fluoro)quinolone antibiotics. $Parallax^{TM}$ kit was also sensitive for the detection of incurred (fluoro)quinolone at Korean Maximum Residual Levels in raw bovine milk as the result of dose response test. Cross reactivities of the antibody with the common (fluoro)quinolones were determined to be norfloxacin, 100%; enrofloxacin, 100%; ciprofloxacin, 100%; danofloxacin, 100%; nalidixic acid, 40%. Lower detection limit (LOD) values of the $Parallax^{TM}$ kit in raw bovine milk were determined to be norfloxacin, 4 ppb; enrofloxacin, 5 ppb; danofloxacin, 5 ppb; ciprofloxacin, 5 ppb and nalidixic acid, 10 ppb. The $Parallax^{TM}$ kit was run 8 times with five different concentrations of norfloxacin to determine the coefficient of variation (CV, %) of intra-assay, which was between 2.7% and 11.8%. To confirm the precision among kit batches for the inter-assay, five different batch kits were tested with 2 different concentration of norfloxacin. The CVs of the inter assay were 4.2% at 50 ppb, and 7.2% at 10 ppb norfloxacin, respectively.

• Journal of Veterinary Clinics
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• v.28 no.5
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• pp.538-541
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• 2011

An 8-year-old spayed Yorkshire Terrier Dog was presented to the Veterinary Teaching Hospital of Kyungpook National University because of the recurrent superficial pyoderma. At the presentation, pustules and papules were present throughout the body. Numerous rods with a few cocci were observed on impression smears and they were confirmed to be Proteus mirabilis and Staphylococcus pseudointermedius consecutively. The patient was treated with systemic enrofloxacin and amoxicillin-clavulanic acid based on the results of antimicrobial sensitivity tests with once a week basis 4% chlorhexidine shampoo. An excellent clinical response was achieved in 2 weeks of therapy and the lesions were fully resolved in 6 weeks. The possibility of P. mirabilis infection should not be overlooked by clinicians in dogs with recurrent superficial pyoderma although it's been considered to be rare.

• Journal of Veterinary Clinics
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• v.16 no.1
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• pp.226-229
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• 1999

A 4-year old male Pitbull terrier dog was presented with a history of nasal discharge of left side and dyspnea for 3 days. This dog had a bitewound on the head due to dog-fighting two weeks ago. There was no abnomality except mild anemic sign on blood examination. Frontal projection of radiographs was taken. This was diagnosed as frontal sinusitis. After skin incision on frontal head, a silicone tube was inserted into the affected frontal sinus. Abscess of the frontal sinus was drainaged and irrigated with saline solution containing trypsin and cephazolin. This patient who also treated with enrofloxacin was recovered completely.

• Korean Journal of Veterinary Service
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• v.31 no.3
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• pp.339-345
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• 2008

This survey was carried out to investigate the infection rate of Riemerella anatipestifer from domestic ducks by isolation. A total of 145 samples (nasal swabs and carcasses) were collected from farms to examine the biochemical properties and antimicrobial susceptibility test from November 2006 to February 2008. Riemerella anatipestifer was isolated from 13 of 145 sample and isolation rate was higher in the ducks below 25 days (76%, 10/13 isolates) than those over 26 days (23%, 3/13). However, there was no significant difference in isolation rate between region. The biochemical properties of isolate were Gram negative (-), non-fermentative rod that grows fastidiously on routine media. In the test of antimicrobial drug susceptibility the isolates were susceptible to tetracycline(100%), enrofloxacin (77%), ampicillin (70%).

• Journal of Veterinary Clinics
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• v.15 no.2
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• pp.378-385
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• 1998

Chloramphenicol주사에 의한 좌골신경손상을 유발한 후 Prednisolone을 이용한 약물처치군, 전침군 및 전침과 약물을 병용한 군에서 각각 회복속도를 비교하였다. 정상보행 횟수, pinching을 통한 감각신경의 통감반응,그리고 병리조직소견 등을 죠사한 결과를 요약 하면 다음과 같다. Enrofloxacin, cefazolin, cephalexin, penicillin 등의 좌골신경주위 주사게 서는 신경마비가 관찰되지 않았으나 chloramphenicol을 주사할 경우에는 좌골신경손상으로 인한 편측성 후지마비가 유발되어 knuckling 반응을 보였다. Chloramphenicol을 투여한후 좌골신경손상에 대한 각 처치에서 정상보행 횟수는 8-12주까지는 침술처치군이 약간 중가하 였으나, 처리군간 유의성은 관찰되지 않았다. 실험기간동안 chloramphenicol 에 의한 신경손 상에 대한 각 처치군별 병리조직 소견은 호전양상이 관찰되지 아니하였다.

• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.475-479
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• 2000

The purpose of this study was to investigate the cause of chronic diarrhea from anaerobic bacteria isolated from Siberian tiger with chronic diarrhea. Major anaerobic bacteria isolated from faces were Clostridium perfringens and their population was $6 {\times} 10^3$ cfu/g feces. Antibiotic sensi- tivity test against Clostridium perfringens was performed using 6 antibiotic drugs including colistin gentamicin, trimethoprim/sulfamethoxazole, amikacin, enofloxacin and penicillin. Clostridium per- fringens were sensitive to amikacin, enrofloxacin and penicillin while they were resistant to colistin. gentamicin and trmetboprim/sulfamethoxazole. And we found that chronic diarrhea of Siberian tiger was treated successfully with penicillin. These results suggested that Clostridium perfringens may bee a cause of chronic diarrhea in Siberian tiger.

• Korean Journal of Veterinary Service
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• v.23 no.3
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• pp.255-262
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• 2000

A survey on pneumonic lungs and its histopathological changes of the slaughtered pigs from the southern area of Chungbuk province was carried out during the period from January to December 1999. Pneumonic lungs were attempted bactenological findings and antibiotic susceptibilities. The results obtained were as follows; 1. Of 158 slaughtered pigs, 97(61.4%) pigs had pneumonic lesions in the lung, and the prevalence was high in winter, spring, autumn, and summer in order. f. The bacteria isolated from pneumonic lesions were pasteurella spp, 13 heads(34.2%), streptococcus spp, 6(IS.8%), actinobacillus spp, 3(7.9%), coliform 4(10.5%) and the other bacteria, 12(31.6%). 3. These isolates were highly susceptible to the antibiotics of enrofloxacin 30(78.9%), cephalothin 23(73.6%) and ceftiofur 27(71 %). 4. Histopathologically, swine enzootic pneumonia and pleuropneumonia lesions were observed. The swine enzootic pneumonia lesions were consisted of peribronchiolar lymphoid hyperplasia and exudate in alveolar lumen. The pleuropneumonia lesions were consisted of thrombosis, alveolar wall thickened by mononuclear cells and neutrophil deposition.

• Korean Journal of Veterinary Service
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• v.16 no.1
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• pp.76-81
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• 1993

To evaluate the pathogenesis of live infectious laryngotracheitis vaccine, virus, virus and E.coli were inoculated in 4-weeks old intraorbitally and intraorally. Their pathology and serology were studied. The results were as follows. 1. thicks Inoculated with ILT vaccine alone showed no clinical sign. 2. Some of chicks inoculated with ILT vaccine followed by E.coli after 1day showed nasal discharge and cough. 3. Some of chicks inoculated with E. coli followed by ILT vaccine after 1 day and inoculated ILT vaccine and E.coli concurrently, showed nasal discharge. 4. Serum neutralization titers of chicks inoculated with ILT vaccine increased 3 weeks after Inoculation, peaked at 7 to 8 weeks and decreased. 5. Antibiotic sensitivity of E.coli were higher in Enrofloxacin (Baytril) and chloramphenical.

• Korean Journal of Veterinary Service
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• v.16 no.2
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• pp.150-156
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• 1993

The present study was conducted to investigate the biochemical characteristics and anti-biotic resistance of pathogenic Escherichia coli (E. coli) isolated from piglets with diarrhea in Kyongbuk Western Area during the period from February to November 1992. 55 E. coli strains were isolated from 97 piglets with diarrhea and the biochemical and cultural reaction were compared with the classification criteria of Edwards and Ewing. The majority of E. coli were susceptible to amikacln, enrofloxacin and gentamicin. 51 (92.7%) out of 55 drug resistance stains carried R factor (R+) which were transferable to the recipients by conjugation.

• Korean Journal of Veterinary Research
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• v.61 no.2
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• pp.16.1-16.6
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• 2021

Pseudomonas aeruginosa is one of the most common pathogenic species associated with canine otitis externa (OE). Their resilience is achieved by forming a biofilm, which allows these bacteria to evade even the harshest of treatments. This study evaluated the in vitro synergistic efficacy of N-acetylcysteine (NAC) with different antimicrobial agents against P. aeruginosa isolated from dogs with OE to develop an effective treatment against P. aeruginosa. The antimicrobial activity was evaluated by the minimum inhibitory concentration test using the microdilution method. The efficacy of antibiofilm formation was evaluated using a crystal violet stain method. The treatment solutions included NAC alone, and in synergy with enrofloxacin, polymyxin B, and gentamicin. NAC alone exhibited antimicrobial and antibiofilm abilities. On the other hand, the combination of NAC and the antibiotics did not show any significant synergistic effects against P. aeruginosa.