• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1390-1393
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• 2003

Measuring in vivo rate of bone collagen synthesis has so far been technically difficult and often subject to quite large errors. In the present study, bone collagen synthesis rate was measured using a precursor-product method, based on the exchange of $^2$$H_2O$ into amino acids. Mass isotopomer abundance in hydroxyproline from bone collagen was analyzed by gas chromatography/mass spectrometry. The $^2$$H_2O$ labeling protocol consisted of an initial intraperitoneal injection of 99.9% $^2$$H_2O$, to achieve approximately 2.5% body water enrichment followed by administration of 4% $^2$$H_2O$ in drinking water for 9 weeks. Body $^2$$H_2O$ enrichments were stable at 2.7 ∼ 3.0% over labeling Period. In growing rats, the fractional synthesis rate ( $k_{s}$) of bone collagen was 0.066 $\pm$ 0.049 w $k^{-1}$ . The unique features of stable $^2$$H_2O$ pools and label incorporation allowed the precursor-product approach to be used for measuring bone collagen synthesis rate..

• Korean Journal of Veterinary Service
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• v.31 no.1
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• pp.1-16
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• 2008

Salmonella infections cause the disease in pigs but also some zoonotic Salmonella serotypes can be transmitted to human through swine products, resulting in food poisoning. The objective of this study was to investigate the bacteriological prevalence and detection of invA gene using Salmonella specific polymerase chain reaction (PCR), the epidemiological characteristics related to Salmonella strains cultured from pig samples in Gangwon areas using serotyping, random amplified polymorphic DNA (RAPD) and pulsed field gel electrophoresis (PFGE) methods. During the period of November 2001 through April 2002, 1,174 ileocecal lymph node were collected from the slaughtered pigs raised in 38 farms located in Gangwon province. The samples were submerged in boiling water and macerated in saline and lymph node homogenates were inoculated into Tetrathionate broth with iodine (TTB, Difco, 0.5% iodine was added) for enrichment growth. Then additional tests were performed using several mediums, and suspects were identified by API 20E kit (BioMerieux) and PCR. Of total 1,174 samples from 38 farms, 44 (3.7%) were isolated as Salmonella spp from 13 farms (34.2%). Of 44 isolates, 31 were in Yangyang region, followed by 9 in Goseong, 2 in both Gangneung and Sokcho. However, there was no difference in regional isolation frequency. All isolates have a 521bp amplified product in Salmonella specific PCR with primer invA which encodes in proteins for invasion of epithelial cells. Of 44 recovered serotypes, 23 (52.3%) were S Eingedi, 10 (22.7%) S Schwarzengrund, 9 (20.5%) S Typhimurium, and 2 (4.5%) S Mbandaka. In RAPD analysis, there appeared to be unique bands distinguishing each serotype, although similarities exist between the different serotypes. Four serotypes of 44 Salmonella isolates appeared to fall into 14 different RAPD types. In PFGE analysis, 9 S Typhimurium were tested with XbaI enzyme and SpeI enzyme. The combination of results obtained with two enzymes subdivided the 9 S Typhimurium into 4 PFGE types.

• Communications of Mathematical Education
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• v.20 no.1 s.25
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• pp.117-145
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• 2006

Mathematically gifted children have creative curiosity about novel tasks deriving from their natural mathematical talents, aptitudes, intellectual abilities and creativities. More effect in nurturing the creative thinking found in brilliant children, letting them approach problem solving in various ways and make strategic attempts is needed. Given this perspective, it is desirable to select open-ended and atypical problems as a task for educational program for gifted children. In this paper, various types of open-ended problems were framed and based on these, teaming activities were adapted into gifted children's class. Then in the problem solving process, the characteristic of bright children's mathematical thinking ability and examples of problem solving strategies were analyzed so that suggestions about classes for bright children utilizing open-ended tasks at elementary schools could be achieved. For this, an open-ended task made of 24 inquiries was structured, the teaching procedure was made of three steps properly transforming Renzulli's Enrichment Triad Model, and 24 periods of classes were progressed according to the teaching plan. One period of class for each subcategories of mathematical thinking ability; ability of intuitional insight, systematizing information, space formation/visualization, mathematical abstraction, mathematical reasoning, and reflective thinking were chosen and analyzed regarding teaching, teaming process and products. Problem solving examples that could be anticipated through teaching and teaming process and products analysis, and creative problem solving examples were suggested, and suggestions about teaching bright children using open-ended tasks were deduced based on the analysis of the characteristic of tasks, role of the teacher, impartiality and probability of approaching through reflecting the classes. Through the case study of a mathematics class for bright children making use of open-ended tasks proved to satisfy the curiosity of the students, and was proved to be effective for providing and forming a habit of various mathematical thinking experiences by establishing atypical mathematical problem solving strategies. This study is meaningful in that it provided mathematically gifted children's problem solving procedures about open-ended problems and it made an attempt at concrete and practical case study about classes fur gifted children while most of studies on education for gifted children in this country focus on the studies on basic theories or quantitative studies.

• 한국해양학회지
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• v.26 no.3
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• pp.204-222
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• 1991

The annual cycles of plant major nutrients and dissolved oxygen in a nutrients-rich semi-enclosed coastal inlet, chinhae Bay, of the southern coast of the Korean Peninsula are first presented. The water column of the bay is stratified during summer (April-late September) and well0mixed during winter (October-March). During the summer stratification period, dissolved oxygen contents exceed 400uM in the surface but diminish to less than 50uM in the near bottom waters, which often results in an anoxic environment in the inner part of Chinhae Bay. After the breakdown of the stratification in October, dissolved oxygen concentration remains undersaturated until February. The evidence of allochthonous input of N-nutrients throughout the year is readily seen in the water column: however. crude budget calculations show that the nutrients are efficiently utilized within the bay ecosystem, and that export of the nutrients from the bay to the shelf must be negligible. There is no sign of the enrichment of the nutrients in the water column. The eutrophication phenomenon sensu stricto is not observed in chinhae Bay. Using the standing stock of dissolved oxygen and estimation of the oxygen fluxes across the air-sea boundary, a benthic oxygen respiration rate during winter is estimated conservatively at 21-24 mmol Cm/SUP -2/d/SUP -1/. this oxygen respiration rate accounts for about 20% of the total phytoplankton production in winter.

• Journal of the Korean Society of Marine Environment & Safety
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• v.27 no.5
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• pp.605-617
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• 2021

The recovery of the sedimentary environment around a fallowing shellfish farm during its rest period after 20 years of long-running operation, was explored in Tongyeong Bay of Korea. Seasonal survey on macrobenthos community was conducted from July 2016 to September 2018 at five stations, which included the fallowing shellfish farm after 20-year operation (FF), a newly installed shellfish farm (NF), a shellfish farm for comparison around the closed shellfish farm (CF), and control stations with no farming activities (Con1, Con2). The total number of species and the density of macrobenthos were higher at the shellfish farm stations than the non-shellfish farm station, and their seasonal changes were significant. The dominant species at the shellfish farm stations was Capitella capitata while Paraprionospio patiens was dominant at the non-shellfish farm stations. The two dominant species were potential organic contamination indicators usually inhabiting the areas with dense organic matter. This suggests that the overall survey area was highly polluted with organic matters. Multivariate analysis showed that the macrobenthic community of CF changed to a level similar to that of Con1 immediately after the investigation, but not to a state above the Con1 level. Furthermore, the changes in NF were not significant when compared to those in CF.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.9
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• pp.1458-1468
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• 2019

Objective: As one of the most important metabolic organs, the liver plays vital roles in modulating the lipid metabolism. This study was to compare miRNA expression profiles of the Large White liver between two different developmental periods and to identify candidate miRNAs for lipid metabolism. Methods: Eight liver samples were collected from White Large of 70-day fetus (P70) and of 70-day piglets (D70) (with 4 biological repeats at each development period) to construct sRNA libraries. Then the eight prepared sRNA libraries were sequenced using Illumina next-generation sequencing technology on HiSeq 2500 platform. Results: As a result, we obtained 346 known and 187 novel miRNAs. Compared with the D70, 55 down- and 61 up-regulated miRNAs were shown to be significantly differentially expressed (DE). Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis indicated that these DE miRNAs were mainly involved in growth, development and diverse metabolic processes. They were predicted to regulate lipid metabolism through adipocytokine signaling pathway, mitogen-activated protein kinase, AMP-activated protein kinase, cyclic adenosine monophosphate, phosphatidylinositol 3 kinase/protein kinase B, and Notch signaling pathway. The four most abundantly expressed miRNAs were miR-122, miR-26a and miR-30a-5p (miR-122 only in P70), which play important roles in lipid metabolism. Integration analysis (details of mRNAs sequencing data were shown in another unpublished paper) revealed that many target genes of the DE miRNAs (miR-181b, miR-145-5p, miR-199a-5p, and miR-98) might be critical regulators in lipid metabolic process, including acyl-CoA synthetase long chain family member 4, ATP-binding casette A4, and stearyl-CoA desaturase. Thus, these miRNAs were the promising candidates for lipid metabolism. Conclusion: Our study provides the main differences in the Large White at miRNA level between two different developmental stages. It supplies a valuable database for the further function and mechanism elucidation of miRNAs in porcine liver development and lipid metabolism.

• Economic and Environmental Geology
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• v.35 no.1
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• pp.25-41
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• 2002

In order to investigate the hydrogeochemical characteristics and contamination of dissolved major ions and heavy metals in the Tancheon River, river water and sediment samples were collected at 18 locations, along a distance of 69 km, between Yongin-si in Kyunggi-do and Samsung-dong in Seoul on October in 2000 and April in 2001. After appropriate sample preparation, waters were analyzed for the dissolved constituents and sediments. The pH values of river waters were in the range of 7.0 to 9.3 and could be plotted in the area of surface environment. The level of $Ca^{2+}$, , CI-, sol-, N0$_{3}$ and HC0$_{3}$ in the Tancheon River were higher than those in world average river water. Most of dissolved constituents in the river waters increased toward downstream from upstream. In particular, high concentrations of Zn2+, Na$_{+}$, CI$^{-}$, SO$_{4}^{2-}$ and N03- were found near densely residential areas and the Sungnam waste water treatment plant. The relative ion enrichment was caused by the inflow of local domestic and industrial sewages. Also, Ca2+ and HC03- concentrations were enriched in the middle of the Tancheon River due to the dissolution of cements. This indicates that the apartment complexes were built on a large scale in the upriver since these ten years and large amounts of construction materials such as cements were flowed into the Tancheon River. Concentrations of heavy metals (Mn, Cd, Cu, Pb, Zn) in sediments from the Tancheon River exceeded the lower limit of tolerence level in bottom sediment established by the Ontario Ministry of the Environment (OME) of Canada. In particular, these metals were highly elevated in sediment (TSM-12) collected from near the Sungnam waste water treatment plant. Heavy metals were higher enriched in sediments collected from dry period rather than wet period.

• Journal of the Korean Society of Marine Environment & Safety
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• v.27 no.7
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• pp.943-953
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• 2021

The purpose of this study is to investigate sediment recovery after the relocation of fish cage farms, by examining the changes in sediments and the benthic polychaete community. A preliminary survey was carried out in October 2017, before the relocation of the farms, and monthly surveys were conducted from November 2017 to October 2018 after the farms were moved. Subsequently, it was conducted every 2-3 months until October 2020. The survey was carried out at three stations (Farm1-3) at the location of the removed fish farms and at three control stations (Con1-3) without farms. The overall organic carbon content of the farm stations was higher than the control stations, but it gradually decreased after the farm was demolished, and there was no statistically significant difference about one year after the relocation of the farms (p<0.05). In the benthic polychaete community, abiotic community appeared at the farm stations in the summer, and consequently, the community transitioned to a low-diversity region with the predominant species Capitella capitata, which is an indicator of pollution. Until the abiotic period in the summer of the next year, the species diversity increased and the proportion of indicator species decreased, showing a tendency of recovering the benthic polychaete community, and these changes were repeated every year. In this study, the abiotic community appeared every year owing to the topographical characteristics, but as the survey progressed, the period of abiotic occurrence became shorter and the process of community recovery progressed expeditiously. Biological recovery of sediments after the relocation of the fish farms is still in progress, and it is imperative to study recovery trends through continuous monitoring.

• Korean Journal of Ecology and Environment
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• v.37 no.1 s.106
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• pp.36-46
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• 2004

We evaluated the effect of limiting nutrients and N/P ratio on the growth of phytoplankton in a small eutrophic reservoir from November 2002 to December 2003. Nutrient limitation was investigated seasonally using nutrient enrichment bioassay (NEB). DIN/DTP and TN/TP ratio (by weight) of the reservoir during the study period ranged 17${\sim}$187 and 13${\sim}$60, respectively. Most of nitrogen in the reservoir account for $NO_3$-N, but sharp increase of ammonia was evident during the spring season. Seasonal variation of dissolved inorganic phosphorus concentration was relatively small. DTP ranged 26.5${\sim}$10.1 ${\mu}g\;P\;L^{-1}$, and the highest and lowest concentration was observed in August and December, respectively. Chlorophyll a concentration ranged 28.8${\sim}$109.7 ${\mu}g\;L^{-1}$, and its temporal variation was similar to that of cell density of phytoplankton. Dominant phytoplankton species were Bacillariphyceae (Melosira varians) and Chlorophyceae (Dictyosphaerium puchellum) in Spring (March${\sim}$April). Cyanophyceae, such as Osillatoria spp., Microcystis spp., Aphanizomenon sp. dominated from May to the freezing time. TN/TP ratio ranged from 46 to 13 (Avg. 27${\pm}$6) from June to December when cyanobacteria (Microcystis spp.) dominated. p limitation for algal growth measured in all NEB experiments (17cases), while N limitation occurred in 8 out of 17 cases. The growth rates of phytoplankton slightly increased with decreasing of DIN/DTP ratio. Evident increase was observed in the N/P ratio of > 30, and it was sustained with DTP increase until 50 ${\mu}g\;P\;L^{-1}$. Under the same N/P mass ratio with the different N concentrations (0.07, 0.7and 3.5 mg N $L^{-1}$), Microcystis spp. showed the highest growth rate in the N/P ratio of< 1 with nitrogen concentration of 3.5 mg N $L^{-1}$). The responses of phytoplankton growth to phosphate addition were clearly greater with increase of N concentration. These results indicate that the higher nitrogen concentration in the water likely induce the stronger P-limitation on the phytoplankton growth, while nitrogen deficiency is not likely the case of nutrient limitation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.4
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• pp.375-383
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• 2009

This study was carried out to develop transgenic rice cultivars with the CAX1 (accession no. U57411) gene. We successfully selected the transgenic rice plants over-expressing the Arabidopsis H+/$Ca^{2+}$ antiporter CAX1 (accession no. U57411) gene in T6 generation. The brown rice of the CAX1 expressing rice contained 13.4~68.0 % more calcium $(Ca^{2+})$ than that of the wild type and 5 lines were selected based on the phenotypes compared to the control cultivar at the GMO field. The CAX1 expressing transgenic rice plants were similar in phenotype to the wild type during the whole growth period. Also these selected 4 lines appeared to be resistant to blast, cold and water solution compared with the wild type. Difference in 1,000 grain weight of brown rice was observed among each line but grain shape did not show any morphological alternations. These results suggest the enhanced Ca-substrate specificity of CAX1 exchanger in donor plant. Therefore, intact CAX1 exchanger can be functionally useful for $Ca^{2+}$ nutrient enrichment of rice with reduced accumulation of undesirable cation.