• Title/Summary/Keyword: enrichment

Search Result 1,860, Processing Time 0.03 seconds

Characterization of Biological Treatment by an Isolated Phenol-Degrading Bacterium (페놀분해세균의 분리 및 생물학적 처리 특성)

  • 송형의;김진욱
    • Journal of Environmental Health Sciences
    • /
    • v.24 no.3
    • /
    • pp.54-62
    • /
    • 1998
  • 20 bacterial strains capable of growing on phenol minimal medium were isolated from soil and wastewater by the enrichment culture technique, and among them, one isolate which was the best in the cell growth was selected and identified as Bacillus sp. SH3 by its characteristics. Strain SH3 could grow with phenol as the sole carbon source up to 15 mM, but did not grow in minimal medium containing above 20 mM of phenol. The optimal conditions of temperature and initial pH for growth and phenol degradation were 30$^{\circ}$C and 7.5, respectively. This strain could grow on various aromatic compounds such as catechol, protocatechuic acid, gentisic acid, o-, m-, p-cresol, benzoic acid, p-hydroxybenzoic acid, anthranilic acid, phenyl acetate and pentachlorophenol, and the growth-limiting log P value of strain SH3 on organic solvents was 3.1. In batch culture, strain SH3 degraded 97% of 10 mM phenol in 48 hours. In continuous culture under the conditions of 20 mM of influent phenol concentration and 0.050 hr$^{-1}$ of dilution rate, the treatment rate of phenol was 94%.

  • PDF

The Contribution and Evaluation to the Studies in Family Resource Management (가족자원관리학 분야 연구의 사회적 기여와 평가)

  • 정영금
    • Journal of Families and Better Life
    • /
    • v.18 no.1
    • /
    • pp.231-242
    • /
    • 2000
  • The purpose of this study is to evaluate the trend of studies in family resource management and the contribution to family and society trough the studies since the establishment of The Korean Home Management Association. For this purpose this study analized 363 articles published in 4 journals relating to family resource management for 2 decades,. The contents are 1) an analysis to the trend of the studies classified by subject and period 2) an analysis to the ratio and trend of practical studies 3) a critic to the problems which this field of studies contains of 4) the suggestions for the future-enlargement of the information and practical education enrichment of education through the field study development of methods and contents for the study.

  • PDF

Geochemical Behavior and Pollution of Soils in Gwangju City (광주광역시 토양의 지화학적 거동 특성과 오염)

  • Shin, Sang-Eun;Kim, Joo-Yong;Oh, Kang-Ho
    • Journal of Environmental Impact Assessment
    • /
    • v.14 no.6
    • /
    • pp.415-425
    • /
    • 2005
  • To examine the geochemical behavior and pollution of soils in Gwangju City, an analysis was carried out for pH, on the contents of metals, and organic carbon. Soil samples were taken from environs areas, industrial areas and downtown areas. The major factor controlling the behavior of metallic elements in the soil was chemical weathering of clay mineral in the environs areas, industrialization, and urbanization. Heavy metals including Cu, Pb and Zn were highly enriched for the samples from central part of downtown area. This indicated that the urbanization and the industrialization affected soil pollution. The results show that soil pollution in a metropolitan city which is caused by harmful heavy metals is severest in the center of the city. In consequence, it is inevitable that practical measures should be taken to prevent soil pollution expansion.

Contamination of Listeria spp. in Market Beef (국내 시판 쇠고기의 Listeria spp. 오염)

  • 구동환;정충일;정동관;남은숙
    • Journal of Food Hygiene and Safety
    • /
    • v.10 no.2
    • /
    • pp.89-95
    • /
    • 1995
  • Highly lethal Listeria monocytogenes, causing bromatoxism through vegetables, dairy products, meat products and shellfish etc, was examined for possible contamination in market beef. USDA, FDA, Malthus and Modified Cold Enrichment methods were used for the detection of Listeria spp.. Samples of domestic and imported market beef were collected from local meat shopsat Seoul, Korea. Total two hundreds and six of Listeria spp. were isolated and identified from beef. Among 206 isolates, the number of L. welshimeri was one hundred and twenty-one(44.8%). The numbers of isolated L. innocua, L. murrayi, L. monocytogenes, L. grayi, L. seeligeri, and L. ivanovii were 49(18.1%), 14(5.2%), 12(4.4%), 6(2.2%), 2(0.7%), and 2(0.7%), respectively. Detection rates of Listeria spp. varied among four methods. The highest detection rate of Listeria spp. in market beef was found at USDA method and that of L. monocytogenes was found at Malthus method.

  • PDF

Simple and Direct Quantitative Analysis for Quinidine Drug in Fish Tissues

  • Chen, Yuan-Chin;Abdelhamid, Hani Nasser;Wu, Hui-Fen
    • Mass Spectrometry Letters
    • /
    • v.8 no.1
    • /
    • pp.8-13
    • /
    • 2017
  • Analysis of quinidine for fish tissues using single drop microextraction (SDME) coupled with atmospheric pressure matrix assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) are reported. Optimization conditions; such as extraction solvent, extraction time, pH of the aqueous solution, salt additions (NaCl), stirring rate, matrix type and concentration are investigated. Linear dynamic range (${\mu}M$), limit of detection, relative recovery%, and enrichment factor are 0.08-9.2, 0.05, $94.8{\pm}3.1-98.5{\pm}3.3%$, $4.34{\pm}0.28-4.40{\pm}0.30$, respectively. SDME-AP-MALDI-MS shows good intraday and interday reproducibility.

Development of a Selective Medium for the Fungal Pathogen Cylindrocarpon destructans Using Radicicol

  • Kang, Yunhee;Lee, Seung-Ho;Lee, Jungkwan
    • The Plant Pathology Journal
    • /
    • v.30 no.4
    • /
    • pp.432-436
    • /
    • 2014
  • The soil-borne ascomycete fungus Cylindrocarpon destructans causes ginseng root rot disease and produces various secondary metabolites such as brefeldin A and radicicol. The slow growth of this fungus compared with other plant pathogenic and saprophytic fungi in soil disturbs isolation of this fungus from soil and infected ginseng. In this study, we developed a selective medium for C. destructans using radicicol produced by this fungus. Supplementing 50 mg/L of radicicol to medium inhibited the mycelia growth of other fungi including Botrytis cinerea, Rhizoctonia solani and Alternaria panax, but did not affect the growth of C. destructans. In addition, conidia germination of other fungal species except for C. destructans was inhibited in submerged culture supplemented with radicicol. This medium provides a very efficient tool for isolating C. destructans and also can be used as an enrichment medium for this fungus.

A Comparative Study of Job Characteristics on Six Sigma Belts and Non Belts: Focusing on Hackman and Oldham's Job Characteristics Model (6시그마 벨트 인증자와 비인증자의 직무특성 및 직무만족 비교연구 : Hackman과 Oldham의 직무특성이론을 중심으로)

  • Yang, Jong-Gon
    • Journal of Korean Society for Quality Management
    • /
    • v.38 no.1
    • /
    • pp.52-63
    • /
    • 2010
  • The purpose of this study is to investigate whether or not belt's jobs represent a source of job enrichment that translates into positive job characteristics and satisfaction. Hackman and Oldham's 5 job characteristics, 3 critical psychological states and MPS are utilized for analyzing results of the study. The results indicate that Black and Master Black Belt's jobs have positive effects on all five job characteristics, 3 psychological states, and MPS score. However, the findings indicate that Black and Master Black Belt's jobs are no direct effect on job satisfaction suggesting that growth need strength controls on job satisfaction. The result shows that there is a support for the prediction that growth needs strength controls the effect of belt's jobs on job satisfaction.

Isolation and Characterization of a Phenol-degrading Strain, Klebsiella pneumoniae (Phenol 분해균주 Klebsiella pneumoniae의 분리 및 특성)

  • 노종수;이헌모
    • Journal of environmental and Sanitary engineering
    • /
    • v.15 no.1
    • /
    • pp.34-38
    • /
    • 2000
  • In the screening of phenol-degrading bacteria, a strain showing good growth in media containing phenol was isolated by using enrichment culture from various sample and identified as genus Klebsiella pneumoniae. The optimal temperature and pH for cell growth of Klebsiella pneumoniae was $35^{\circ}C$ and 8.0, respectively. When phenol was added to the minimal media as a sole source of carbon and energy, the concentration of maximum and optimum for cell growth was 1,200ppm and 1,000ppm, respectively. It was observed that Klebsiella pneumoniae was able to degrade 98% of phenol (1,000ppm) after 40hr in culture. The isolated could utilize various kinds of aromatic compounds and showed good growth in presence of phenol, m-cresol and 3-methyl catechol.

  • PDF

Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces

  • Kim, Yong Kwan;Kim, Ha-Young;Jeon, Albert Byungyun;Lee, Myoung-Heon;Bae, You-Chan;Byun, Jae-Won
    • Korean Journal of Veterinary Research
    • /
    • v.54 no.2
    • /
    • pp.113-115
    • /
    • 2014
  • Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were $10^4$, $10^5$ and $10^2$ CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.

Isolation and Identification of Pseudomonas Utilizing Hydrocarbon (탄화수소를 자화하는 Pseudomonas의 분리동정)

  • Kim, Jeong-Kook;Lee, Yung-Nok
    • Korean Journal of Microbiology
    • /
    • v.22 no.1
    • /
    • pp.29-34
    • /
    • 1984
  • 238 strains of bacteria were isolated from sewage and soil samples collected mainly in Seoul and its suburbs by enrichment culture on crude oil or hydrocarbon minimal medium. Of the isolates, 68 strains were tentatively identified as the genus Pseudomonas, 11 strains as Alcaligenus, and 10 strains as Acinetobacter. Of the 68 strains of Pseudomonas sp., 35 strains were identified as P. aeruginosa, 5 strains as P. fluorescence, 10 strains as P. putida, and 2 strains as P. mendocina.

  • PDF