Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces

  • Kim, Yong Kwan (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Kim, Ha-Young (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Jeon, Albert Byungyun (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Lee, Myoung-Heon (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Bae, You-Chan (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Byun, Jae-Won (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
  • Received : 2013.08.07
  • Accepted : 2014.02.28
  • Published : 2014.06.30
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Abstract

Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were $10^4$, $10^5$ and $10^2$ CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.

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References

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