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http://dx.doi.org/10.14405/kjvr.2014.54.2.113

Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces  

Kim, Yong Kwan (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Kim, Ha-Young (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Jeon, Albert Byungyun (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Lee, Myoung-Heon (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Bae, You-Chan (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Byun, Jae-Won (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Publication Information
Korean Journal of Veterinary Research / v.54, no.2, 2014 , pp. 113-115 More about this Journal
Abstract
Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were $10^4$, $10^5$ and $10^2$ CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.
Keywords
detection limit; invA; loop-mediated isothermal amplification assay; Salmonella;
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