• Korean Journal of Veterinary Research
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• v.45 no.2
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• pp.191-197
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• 2005

An immunochromatographic (IC) strip for the rapid detection of Salmonella spp. in the enriched sample was developed. Affinity purified Salmonella polyclonal antibody was conjugated with 40 nm colloidal gold particles which were prepared by citrate method in our laboratory. The antigen-antibody-gold complex was captured by Salmonella antibody attached to test line of nitrocellulose membrane during the capillary migration of sample. Specificity of the IC strip was calculated to be 100% (12/12) and sensitivity was 97.6% (41/42) in the test with pure cultured bacteria. Salmonella was artificially inoculated into raw pork macerated with enrichment broth. And then it was 10-fold diluted from $5.2{\times}10^{8}CFU/ml$ to 5.2 CFU/ml. The IC strip could detect $5.2{\times}10^{6}CFU/ml$ before enrichment. However, the lowest limit of detection was 5.2 CFU/ml after overnight incubation. The results indicated that the IC assay was a rapid, economical and simple method with high specificity and sensitivity for the detection of Salmonella spp. without using any equipment.

• Journal of Environmental Science International
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• v.19 no.7
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• pp.799-806
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• 2010

In an effort to characterize temporal and spatial variability of $PM_{10}$ and to quantitatively estimate contribution of sea salt aerosol to $PM_{10}$ mass in Busan area, twenty four-hour averaged concentration of $PM_{10}$ were measured in two distinct areas, Gwaebeopdong(inland) and Dongsamdong(seashore), Busan for summer and fall, 2007. It was found that sea salt accounted for 2.9% and 9.5% of $PM_{10}$ mass in Gwaebeopdong and Dongsamdong, respectively for the study period, indicating that contribution of sea salt to $PM_{10}$ mass and total ion concentration in seashore area were consistently higher by a factor of three compared to inland area. Temporal analysis suggested that sea salt contributions to $PM_{10}$ in Dongsamdong were higher in summer due to the southerly sea breeze while there was no significant fluctuation of sea salt contribution for the summer and fall months in Gwaebeopdong. Sea salt enrichment factors($EF_{sea}$) of $K^+$ $Ca^{2+}$ and ${SO_4}^{2-}$ (>10) indicated major contributions from anthropogenic sources and EFs of $Mg^{2+}$ and $Cl^-$ exhibited strong association with oceanic origins for both areas.

• Journal of Korea Game Society
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• v.18 no.1
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• pp.63-72
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• 2018

Legal issues whether augmented reality(AR) game service companies are responsible for damages that their game users cause to the community and residents are occurring these days. These kind of damages affect not only game users but also third parties who do not play the AR game. This paper explores the recent cases and analyze the type of legal issues such as trespass, nuisance and unjust enrichment. To determine the necessity of regulating the AR game company, constitutional balancing test, causation, specificity and persistence of the damage and other various standards should be applied.

• Bulletin of the Korean Chemical Society
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• v.32 no.10
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• pp.3561-3565
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• 2011

A rapid and inexpensive method was developed for the determination of trace silver in geological samples by using sulfhydryl cotton coupled with ICP-MS. The interferences such as $^{90}Zr$, $^{92}Mo$ and $^{93}Nb$ on silver were investigated in detail. Sulfhydryl cotton was found to be an effective adsorbent for separation of interferences for Ag in the solutions. Excellent agreements with the certified values were obtained for all the certified reference materials. The memory effects of Ag by ICP-MS were examined by using different agents, including water, nitric acid, and HCl-thiourea to all standards/samples. The agents also acted as cleansing solutions. A combination of HCl with thiourea gave the minimum memory effect. For comparison of results, a proposed Chinese Geology Survey procedure DC-ARC-AES and a direct determination pretreatment method of ICP-MS (water bath- auqa regia digestion) were studied. Under optimal conditions, the detection limits of our method for $^{107}Ag$ and $^{109}Ag$ were 1.2 ng/g and 1.3 ng/g, which offered much better accuracy for some difficult analysis geological samples such as GBW07604, GBW07605.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.14 no.3
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• pp.381-390
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• 2001

In this paper, a new improved crack analysis technique by Element-Free Galerkin(EFG) method is proposed, in which the singularity and the discontinuity of the crack successfully described by adding enrichment terms containing a singular basis function to the standard EFG approximation and a discontinuity function implemented in constructing the shape function across the crack surface. The standard EFG method requires considerable addition of nodes or modification of the model. In addition, the proposed method significantly decreases the size of system of equation compared to the previous enriched EFG method by using localized enrichment region near the crack tip. Numerical example show the improvement and th effectiveness of the previous method.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.74.2-74.2
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• 2012

Metallicity is an important tracer of star formation in galaxy evolution. Based on the flux ratios of broad emission lines, AGN metallicity has shown a correlation with AGN luminosity. However, it is not clear what physical parameter drives the observed correlation. Using a sample 69 Palomar-Green QSOs at low-z (z<0.5), we determine BLR gas metallicity from emission line flux ratios, i.e., N V1240/C IV1549, (Si IV1398+O IV1402)/C IV1549 and N V1240/He II1640 based on the UV spectra from the HST and IUE archives. We compare BLR gas metallicity with various AGN properties, i.e., black hole mass, AGN luminosity and Eddington ratio, in order to investigate physical connection between metal enrichment and AGN activity. In contrast to high-z QSOs, which show the correlation between metallicity and black hole mass, we find that the metallicity of low-z QSOs correlates with Eddington ratio, but not with black hole mass, suggesting that metallicity enrichment mechanism is different between low-z and high-z.

• Journal of the Korean Ceramic Society
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• v.51 no.6
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• pp.570-574
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• 2014

Magnetic-graphene nanosheets have been synthesized via a simple effective chemical precipitation method followed by heat treatment. The composite nanosheets are super paramagnetic at room temperature and can be separated by an external magnetic field. The prepared magnetic-graphene nanosheets were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, and BET surface area analysis. The results demonstrated the successful attachment of iron oxide nanoparticles to graphene nanosheets. It was found that the attached nanoparticles were mainly $Fe_3O_4$. The magnetic-graphene nanosheets showed near complete methyl orange removal within 10 mintues and would be practically usable for methyl orange separation from water.

• IMMUNE NETWORK
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• v.11 no.6
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• pp.406-411
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• 2011

Background: Invariant Natural killer T (iNKT) cells, a distinct subset of CD1d-restricted T cells with invariant $V{\alpha}{\beta}$ TCR, functionally bridge innate and adaptive immunity. While iNKT cells share features with conventional T cells in some functional aspects, they simultaneously produce large amount of Th1 and Th2 cytokines upon T-cell receptor (TCR) ligation. However, gene expression pattern in two types of cells has not been well characterized. Methods: we performed comparative microarray analyses of gene expression in murine iNKT cells and conventional $CD4^+CD25^-$ ${\gamma}{\delta}TCR^-$ T cells by using Gene Set Enrichment Analysis (GSEA) method. Results: Here, we describe profound differences in gene expression pattern between iNKT cells and conventional $CD4^+CD25^-$ ${\gamma}{\delta}TCR^-$ T cells. Conclusion: Our results provide new insights into the functional competence of iNKT cells and a better understanding of their various roles during immune responses.

• Genomics & Informatics
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• v.15 no.2
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• pp.56-64
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• 2017

We have previously reported that NS-398, a cyclooxygenase-2 (COX-2)-selective inhibitor, inhibited replicative cellular senescence in human dermal fibroblasts and skin aging in hairless mice. In contrast, celecoxib, another COX-2-selective inhibitor, and aspirin, a non-selective COX inhibitor, accelerated the senescence and aging. To figure out causal factors for the senescence-modulating effect of the inhibitors, we here performed cDNA microarray experiment and subsequent Gene Set Enrichment Analysis. The data showed that several senescence-related gene sets were regulated by the inhibitor treatment. NS-398 up-regulated gene sets involved in the tumor necrosis factor ${\beta}$ receptor pathway and the fructose and mannose metabolism, whereas it down-regulated a gene set involved in protein secretion. Celecoxib up-regulated gene sets involved in G2M checkpoint and E2F targets. Aspirin up-regulated the gene set involved in protein secretion, and down-regulated gene sets involved in RNA transcription. These results suggest that COX inhibitors modulate cellular senescence by different mechanisms and will provide useful information to understand senescence-modulating mechanisms of COX inhibitors.

• Journal of Soil and Groundwater Environment
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• v.19 no.1
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• pp.8-25
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• 2014

The aims of this study were to determine concentrations of selected metals in Asian and non-Asian dust collected in Daejeon, Korea between February 2008 and December 2008 and to estimate the pollution level. The geochemical analyses of Asian dust (AD) and Non Asian dust (NAD) show that the mean concentrations of As, Cd, Cu, Pb, Zn, Zr, Sb, Mo and S reached levels up to 16, 209, 31, 43, 81, 28, 31, 122 and 302 times higher, respectively, than those in uncontaminated Chinese desert soils. These results indicate that both AD and NAD serve as an atmospheric repository for trace and heavymetal accumulation. The the enrichment factor (EF) and pollution index (PI) show that AD and NAD were severely contaminated by S, Mo, Zr, Cd, Pb, Zn, Sb, Cu, and As. All indices for these metals showed either strong or notably high level of pollution relative to Chinese desert soil, principally due to the severe atmospheric pollution derived from anthropogenic activities in heavily industrial Chinese cities. Therefore, Mo, Cd, Zr, As, Cu, Sb, Pb, and Zn are the ones most strongly affected by anthropogenic inputs such as airborne pollutants.