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The Korean Association of Immunobiologists (대한면역학회)
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Comparison of Invariant NKT Cells with Conventional T Cells by Using Gene Set Enrichment Analysis (GSEA)

  • Oh, Sae-Jin (Department of Pathology, Laboratory of Immune Regulation in Department of Biomedical Sciences, Seoul National University College of Medicine) ;
  • Ahn, Ji-Ye (Department of Pathology, Laboratory of Immune Regulation in Department of Biomedical Sciences, Seoul National University College of Medicine) ;
  • Chung, Doo-Hyun (Department of Pathology, Laboratory of Immune Regulation in Department of Biomedical Sciences, Seoul National University College of Medicine)
  • Received : 2011.11.02
  • Accepted : 2011.11.23
  • Published : 2011.12.31
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Abstract

Background: Invariant Natural killer T (iNKT) cells, a distinct subset of CD1d-restricted T cells with invariant $V{\alpha}{\beta}$ TCR, functionally bridge innate and adaptive immunity. While iNKT cells share features with conventional T cells in some functional aspects, they simultaneously produce large amount of Th1 and Th2 cytokines upon T-cell receptor (TCR) ligation. However, gene expression pattern in two types of cells has not been well characterized. Methods: we performed comparative microarray analyses of gene expression in murine iNKT cells and conventional $CD4^+CD25^-$ ${\gamma}{\delta}TCR^-$ T cells by using Gene Set Enrichment Analysis (GSEA) method. Results: Here, we describe profound differences in gene expression pattern between iNKT cells and conventional $CD4^+CD25^-$ ${\gamma}{\delta}TCR^-$ T cells. Conclusion: Our results provide new insights into the functional competence of iNKT cells and a better understanding of their various roles during immune responses.

Keywords

References

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