• 한국환경과학회지
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• 제11권2호
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• pp.131-137
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• 2002

The chemical behavior and properties on the redox state of environmental pollutant has been investigated by electrochemical methods. We carried out to measure the variations in the redox reaction of differential pulse polarogram and cyclic voltammogram. The results observed the influences on redox potential and current of various factors with temperature and pH. These were established factors as the effect of the redox reaction. It can be clearly recognized that the electrode reaction are from qusi-reversible to irreversible processes. Also, it was mixing with reaction current controlled. The bits-phenol A in the waste water was made to compound with cobalt ion and it take away from the separation into compound. The $Co(BPA)_2$ compound was not found to be dissociation in waste water. However, this compound is avery unstable(K=1.02) and for a while, it was to be a dissociation. Therefore, we believed that it was likely to a toxic substance.

• 한국물환경학회지
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• 제37권6호
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• pp.501-509
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• 2021

In 2018, total 263 micro and emerging contaminants were selected as target substances by the Ministry of Environment, and 80 of them were first-class substance including endocrine disruptors, residual Pharmaceuticals and Personal Care Products (PPCPs), residual organic pollutants, pesticides and heavy metals. In this study, in order to evaluate the Hazard Quotient (HQ) of the 80 types in the domestic water environment the concentration of discharged effluent and nearby water environment reported by Korean institutes since 2010 was investigated. There were 45 substances reported to be detected, and Measurement Environment Concentration (MEC) were obtained by collectively converting them into water environment concentration. For biotoxicity, half maximal Effective Dose (EC50) to Daphnia magna, a water fleas species widely adopted in Material Safety Data Sheet (MSDS) was applied. As for the biotoxicity level, the Predicted No-Effect Concentration (PNEC) was obtained by applying the Assessment Factor (AF) and the HQ was derived by dividing it from the MEC. As a result of calculating the HQ, more than 1 substances were Cabamazepine, Mefenamic acid, Acetaminophen, Ibuprofen, Nonylphenol, Nickel, Erythromycin, Acetylslic acid, etc. Meanwhile, perfluorinated compounds were identified as hazardous substances in the water env ironment, with 5 out of 14 species included in the 20 ranks of first-class substance.

• 환경생물
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• 제20권2호
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• pp.158-164
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• 2002

합성화학물질의 몇몇은 고등동물의 내분비계를 교란하는 물질로 작용하는 것이 알려져 있다. 다이옥신, 디디티, 피시비 및 비스페놀 에이 등이 내분비계 장애물질로 분류되어 선진 각국에서는 엄격한 통제하에 관리되고 있다. 본 연구는 자주달개비 미세핵 분석법을 이용하여 염색체이상 유발에 있어서의 BPA와 방사선의 상대적 효과를 비교하기 위하여 시행되었다. 홉수처리를 위하여 자주달개비 4430클론의 절취화서를 각각의 농도로 조제된 BPA용액에 여섯시간 동안 침지하고 폭기를 실시하였다. 한편 방사선의 영향을 평가하기 위하여 동일 식물체의 화서군을 양액에 침지한 상태로 0-0.5 Gy까지 선량별로 조사하였다. 실험선량의 범위에서 미세핵 생성률은 분명한 선량-반응 관계를 나타내었으며 BPA 실험농도 범위에 대해서도 농도-반응 관계를 나타내었다. 이들 두 가지 반응 관계식을 이용하여 동일한 미세핵 빈도를 유발하는 방사선량과 비스페놀 에이 농도를 산출할 수가 있는데, BPA $ll.8\mu{M}$은 방사선 피폭 후 임상증상이 나타나기 시작하는 선량인 0.25 Gy에 상응하는 염색체 이상을 유발하는 것으로 확인되었다 본 연구의 결과를 통하여 자주달개비 화분모세포는 비스페놀 에이, 옥틸페놀 또는 노닐페놀 등의 내분비계 장애물질의 생물학적 독성을 평가하는데 유용하게 활용할 수 있는 생물말단 점이라는 것이 입증되었다.

• 생명과학회지
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• 제26권11호
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• pp.1345-1354
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• 2016

원형탈모증은 흔하게 발병하고 두피와 전신에 모발의 탈락을 일으키는 모낭조직 특이 자가면역질환이다. 모낭은 자체적으로 면역체계와 내분비 환경을 가지고 각 모발주기 단계에 따라 다른 면역 상태를 나타내는 특이한 기관이다. 성장기 모낭의 면역특권의 파괴는 모낭상피 MHC class I 발현과 자가반응성 CD8+T세포에 대한 자가항원 발현을 유도하는 자가면역의 공격을 일으키고 원형탈모증을 유발한다. 임상적 실험적 연구에 의하면, 심리적 스트레스도 모낭 면역/호르몬 체계에 영향을 미쳐 원형탈모증의 유도에 관여할 수 있다고 지적한다. 원형탈모증의 핵심적인 병리기전은 면역특권 수호자(ACTH, ${\alpha}-MSH$와 $TGF-{\beta}$ 등), 자연살해세포그룹 2D-양성(NKG2D+) 세포(NK 세포와 CD8+T 세포 등)와 스트레스 호르몬(CRH와 substance P)과 관련되어 있다. 효과적인 치료법은 여전히 요구되고 있다. 앞으로 치료목표 중의 하나는 스트레스를 포함한 모낭 면역특권을 개선하는 것일 것이다. 최근 연구는 건선, 아토피피부염, 류마티스 관절염 같은 다른 자가면역질환에서 사용되는 JAK억제제와 면역조절제, Tregs, 혈소판풍부혈장요법, 스타틴과 프로스타글란딘 유사제가 원형탈모증에 효과적이라고 보고하였다. 본 논문은 모낭주위 내분비/면역과 관련된 발병기전에 대한 새로운 이해와 원형탈모증의 새로운 치료법에 대해 고찰하였다.

• 대한수의학회지
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• 제41권4호
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• pp.477-484
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• 2001

붕어 (Carassius auratus Linnaeus)의 장에 존재하는 소화관 내분비세포의 부위별 분포 및 출현빈도를 포유류의 peptide에 대한 10종류의 항혈청을 사용하여 면역조직화학적 방법으로 관찰하였다. 붕어의 장은 근위부에서부터 원위부까지 5 등분했으며 (Segments I~V), 대부분의 면역반응세포들은 상피부분의 상피세포들 사이 공간에서 관찰되었으며, 장 내강까지 신장되어 있는 긴 세포질돌기를 함유한 방추형의 개방형 세포 (open type cell)들이 주로 관찰되었으나, 세포질돌기 없이 원형 또는 타원형의 형태를 나타내는 폐쇄형 세포 (close type cell)들 역시 소수 관찰되었다. 본 실험에서는 somatostatin, cholecystokinin (CCK)-8 및 pancreatic polypeptide (PP) 면역반응세포들이 관찰되었으나, serotonin, glucagon, chromogranin A, secretin, vasoactive intestinal peptide (VIP), substance P 및 bombesin 면역반응세포들은 관찰되지 않았다. 한편 somatostatin 면역반응세포들은 장의 가장 근위부인 Segment I에 국한되어 극소수 관찰되었으며, CCK-8 면역반응세포들은 장의 근위부인 Segment I과 II에서 소수 또는 극소수의 빈도로 관찰되었다. PP 면역반응세포들 역시 장의 근위부에서 중간 부위인 Segment I에서부터 III에 걸쳐 중등도 또는 극소수의 빈도로 출현하였다. 이상에서 붕어 위 내분비세포들의 부위별 분포 및 출현 빈도는 다른 위가 없는 경골어류 (stomachless teleost)에서의 보고들과 유사하였으나, 독특한 분포를 나타내는 면역반응세포들 역시 관찰되었다.

• Environmental Engineering Research
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• 제23권1호
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• pp.28-35
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• 2018

$17{\alpha}-ethinylestradiol$ (EE2), a synthetic estrogen which interfere the endocrine and reproductive function in living organisms, has been found extensively to be deposited into municipal wastewater treatment plants and the environment via human excretion. EE2 has long been known to be efficiently cometabolized by ammonia-oxidizing bacteria (AOB) during ammonia ($NH_3$) oxidation. Current study aims to investigate the effect of culture history on the biotransformation of EE2 by nitrifying sludge which was enriched under different ammonia loading rates in continuous flow reactors. Result showed that past growth condition largely affected not only the metabolic rate of $NH_3$ oxidation but also EE2 cometabolism. Sludge previously acclimated with higher $NH_3$ loads as well as sludge dominated with AOB belong to high growth cluster (Nitrosomonas europaea-Nitrosococcus mobilis) showed higher rate of EE2 biotransformation than those one being acclimated with lower $NH_3$ loads because of its ability to provide more reducing power from $NH_3$ oxidation. Moreover, the correlation between the degradation rates of $NH_3$ and EE2 was higher in sludge being acclimated with higher load of $NH_3$ in comparison with other sludge. Implication of the findings emphasized the role of volumetric $NH_3$ loading rate in determining EE2 removal in wastewater treatment system.

• Asian-Australasian Journal of Animal Sciences
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• 제20권7호
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• pp.1156-1168
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• 2007

The somatotropic (GH-IGF-I) axis consists of many hormonal and nutritional factors that control GH release from the somatotrophs in the anterior pituitary. The GH-releasing substances are GHRH and GHS (GHRP or ghrelin), while the GH release-inhibiting substances are somatostatin (SRIF), insulin-like growth factor-I (IGF-I), leptin and glucocorticoids. However, there is evidence showing that nutrition is involved in the control of the somatotropic axis. In addition, weaning is a drastic event for neonates because their alimentary and endocrine circumstances are changed due to the switch, even if gradual, from a liquid milk diet to one composed of such solids as hay and grains. The biological role of ghrelin is one of the hormonal factors that have been focused on ever since ghrelin was discovered at the end of the last century. A 27-amino acid peptide that is mainly synthesized and released from the abomasum epithelium, ghrelin has not been fully evaluated in relation to the somatotropic axis of the ruminant. It has also proven difficult even to investigate the cellular mechanisms of ghrelin action, because this hormone exerts animal-species-dependent actions via a complex set of intracellular signaling pathways. This is also the case for the action of leptin. Another substance, IGF-I, shows a partial inhibitory action on GH secretion in the ruminant. The effect of nutrition is also different among animal species. This is evident by the fact that undernutrition suppresses the circulating GH levels in rodents, but increases it in ruminants and humans. Recently, weaning has been shown to change the postprandial GH responses in ruminants; milk feeding increases, but hay and concentrate feeding suppress, the postprandial circulating GH levels. Even if the postprandial GH level is increased, the ghrelin level is decreased by milk feeding. Macronutrients also possess stimulatory and inhibitory actions on GH secretion in vivo and in vitro. These findings indicate the complexity of the control mechanisms of the somatotropic axis. In the present review, we summarize recent findings on the factors controlling the axis of the ruminant.

• Biomolecules & Therapeutics
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• 제8권1호
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• pp.78-83
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• 2000

The objective of this study was to prevalidate the Organization for Economic Cooperation and Development's (OECD) rodent uterotrophic assay as a test method for screening of potential endocrine disrupting chemicals (EDCs). This study was conducted exactly as described in the OECD protocol documents. A positive control substance, 17$\alpha$-ethinyl estradiol (EE), was administered daily for three days to ovariectomized (OVX) Sprague-Dawley rats at various doses for determine the dose-response curve. Additionally, a pure antiestrogenic chemical, ZM189, 154 was administered to OVX rats at the same time EE to determine the effectiveness of the material against blocking the estrogenic effects of EE. At higher concentration of EE (10 $\mu\textrm{g}$/kg), a statistically significant difference in body weight gain and food consumption was observed compared to vehicle controls. In uterine responses, EE produced a dose-related increase in uterus weights compared to vehicle control. These increases were statistically significant at the >1.0 $\mu\textrm{g}$/kg doses. However, a similar dose-response relationship was not observed in vagina weight. A comparison of the two groups receiving ZM189,154 (0.1 and 1.0 mg/kg) with 0.3 $\mu\textrm{g}$/kg of EE and the group receiving only 0.3 $\mu\textrm{g}$/kg of EE showed dose-related decreases in uterus weights. However, statistical significance was shown in 1.0 mg/kg of ZM189,154. In conclusion, administration of EE produced a dose-related increase in uterine (wet and blotted) weights. Additionally, the 1.0mg/kg dose of ZM189,154 was effective in blocking the estrogenic activity of EE. These data suggest 3-day uterotrophic assay using OVX rats may serve as a good tool for EDCs screening.

• 환경정책연구
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• 제13권2호
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• pp.65-98
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• 2014

해양환경의 플라스틱은 풍화작용에 의해 매우 작은 크기로 부서지는데, 이를 미세플라스틱이라고 한다. 해수에 오염된 잔류성이 크거나 생물축적성이 있는 유기물질은 미세플라스틱에 잘 흡착되며, 이들 물질이 흡착된 미세플라스틱을 해양생물이 섭취하면 먹이사슬을 통해 점차 생물축적된다. 이는 결국 해양생태계의 건강성을 파괴하고 사람을 포함한 상위포식자에게 피해를 끼친다. 해양 오염물질의 흡착제로서의 역할뿐 아니라, 미세플라스틱 그 자체에서 내분비계 교란물질이 용출되어 해양생물에 피해를 입히기도 한다. 우리나라는 잔류성이나 생물축적성이 큰 물질에 대해서는 화학물질 측면에서 규제하고 있으나, 이는 육상에서의 관리에 국한되어 있다. 만약 이들 물질이 해양생태계로 유입되면, 결국 미세플라스틱 흡착 여부에 의해 그 피해가 증가하게 된다. 이에 해양생태계 내에서의 잔류성 생물축적성 물질의 피해를 저감하기 위해서는 우선 미세플라스틱의 관리가 엄격해야 할 것이다. 이에 미세플라스틱 자체를 유해물질로 지정하여 엄격히 관리하거나, 생분해성 플라스틱 이용 확대, 재활용 및 재사용 촉구, clean-up 프로그램의 확대 등을 통해 해양생태계 내로의 플라스틱 유입을 저감하는 노력이 요구된다.

• 한국산업보건학회지
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• 제32권3호
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• pp.253-267
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• 2022

Objectives: This study was performed to check whether the CRS (Chemical Ranking and Scoring) system is appropriate as a method to determine substances as candidates for substances subject to permission and to apply this system to the selection of candidates for substances subject to permission. Methods: A risk score was obtained by multiplying the hazard score and the exposure score and then ranking them. The hazard sub-indicators are carcinogenicity, germ cell mutagenicity, reproductive toxicity, specific target organ toxicity-repeated exposure, respiratory sensitization and endocrine disrupting chemicals. Exposure sub-indicators are persistence, bioaccumulation and emission volume. Sensitivity analysis was performed for missing values. Correlation analysis and multivariable linear regression analysis were performed among hazard, exposure and risk in order to confirm that CRS was an appropriate method. Results: As a result of the sensitivity analysis on missing values, it was confirmed that the effect on the risk ranking was not sensitive. Correlation and regression analysis confirmed that exposure had a greater effect on risk than hazard. Conclusions: The CRS system, which derives a risk score using a hazard and exposure score, is judged to be appropriate as a method for the selection of preliminary of candidates for substances subject to permission. Benzene, cadmium, nickel, and cobalt were selected as priority candidates for substances subject to permission.