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Comparative Study on Biological Effects of Gamma-Radiation and Bisphenol A with Tradescantia Micronucleus Assay  

신해식 (충남대학교 환경공학과)
송희섭 (한국원자력연구소)
현성희 (을지의과대학교)
이진홍 (충남대학교 환경공학과)
김진규 (한국원자력연구소)
Publication Information
Korean Journal of Environmental Biology / v.20, no.2, 2002 , pp. 158-164 More about this Journal
Abstract
Some of synthetic chemicals can net as an endocrine disrupting substance in higher animals. Dioxins, DDT, PCBs and bisphenol A (BPA) are classified into endocrine disruptors and aye under a strict control in many countries. This research was designed to compare the clastogenic effects of BPA to those of ionizng radiation to establish the relaltive effectiveness of BPA by means of Tradescantia micronucleus assay. For the uptake of the BPA through the stems, groups of fresh cuttings of Tradescantia BNL 4430 weve placed in BPA solutions of 0 to 4 $\mu$M for 6 hours under continuous aeration. The other groups of the cuttings were irradiated with 0 to 0.5 Gy of gamma- rays. The frequencies of micronucleus showed a positive dose- response relationship in the range of 0 to 0.5 Gy, and a clear concentration-response relationship in the experimental range of BPA concentrations. By comparing the two experimental results, it is possible to estimate the BPA concentration and its equivalent radiation dose for a fixed value of MCN frequency. BPA of $ll.8\mu{M}$ can give rise to 53.3 MCN/100 tetrads, which is the same frequency induced by 25 cGy of gamma- rays. It is of biological importance that clinical symptoms start to develop after a whole body exposure to radiation higher than 25 cGy. The results indicate that the pollen mother cells are an excellent biological end- point for toxicity test of suspected endocrine disrupting chemicals such as bisphenol A, cotylphenol and nonylphenol.
Keywords
Radiation; Bisphenol A; Tradescantia; Micronucleus;
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