• The Journal of the Korea Contents Association
• /
• v.9 no.8
• /
• pp.285-293
• /
• 2009

The purpose of this study is to closely examine the effect of exercise behavior change of casino securities on their Self-efficacy. This observation takes place in casino enterprises in the whole country. Within these areas, we set the selected employees as the targeted sampling unit, we extracted the specimen, using the stratified cluster random sampling with the quota sampling, putting a weigh on the specimen of focused areas. Among 420 persons sampling unit, we have excluded 47 copied which seem to be insincere, and actually used 373 copies in this study. Evaluation forms are used as a study method; each form consists of continuance 5 points Likert scales and nominal/proportional scaling and used after excluding a test through the analysis of validity and reliability. After encoding and inputting the framing completed data along with each purpose, it was computerized by computer process, making use of SPSS 15.0 version. Through the data analysis according to these methods and procedures, the result on this study is described below. First, the exercise behavior change process the self-efficacy according to socio-demographic characteristics make a difference. Second, the self-efficacy according to socio-demographic characteristics make a difference. Third, the exercise behavior change process influence on the self-efficacy.

• The Journal of the Korea Contents Association
• /
• v.16 no.2
• /
• pp.287-294
• /
• 2016

The whole design process drawing outputs from design planning is based on designers' intuitive ideas and differences in technical ability. However, every design professional agrees to the fact that such design process is filled with individuals' implicit acts or inherent intuition rather than following directions in a certain defined frame. If the design process based on such intuition and experience can start from the rational and scientific process, it would be possible to move more systematic design process to the next step. This study aims to examine what system of thinking are used by designers to conduct the design information process, by using the protocol analysis which is the representative qualitative study method of cognitive science, on designers' cognitive behaviors like the contents of the design sketch process which is the initial step of the kitchenware development process, in order to understand professionals' and non-professionals' design application direction. Thus, the scope of this paper would be limited to "the initial protocol analysis on kitchenware" and "encoding of the analysis on thinking" in the qualitative study that can be the most basic to understand the design cognitive science. As a procedure to solve this study question, it aimed to seek for the product development flow and the educational effect by considering the preceding researches and also re-interpreting cognitive thinking in systematic methods.

• Journal of Microbiology and Biotechnology
• /
• v.28 no.4
• /
• pp.588-596
• /
• 2018

An endo-${\beta}$-1,4-glucanase gene, cel9K, was cloned using the shot-gun method from Paenibacillus sp. X4, which was isolated from alpine soil. The gene was 2,994 bp in length, encoding a protein of 997 amino acid residues with a predicted signal peptide composed of 32 amino acid residues. Cel9K was a multimodular enzyme, and the molecular mass and theoretical pI of the mature Cel9K were 103.5 kDa and 4.81, respectively. Cel9K contains the GGxxDAGD, PHHR, GAxxGG, YxDDI, and EVxxDYN motifs found in most glycoside hydrolase family 9 (GH9) members. The protein sequence showed the highest similarity (88%) with the cellulase of Bacillus sp. BP23 in comparison with the enzymes with reported properties. The enzyme was purified by chromatography using HiTrap Q, CHT-II, and HiTrap Butyl HP. Using SDS-PAGE/activity staining, the molecular mass of Cel9K was estimated to be 93 kDa, which is a truncated form produced by the proteolytic cleavage of its C-terminus. Cel9K was optimally active at pH 5.5 and $50^{\circ}C$ and showed a half-life of 59.2 min at $50^{\circ}C$. The CMCase activity was increased to more than 150% in the presence of 2 mM $Na^+$, $K^+$, and $Ba^{2+}$, but decreased significantly to less than 50% by $Mn^{2+}$ and $Co^{2+}$. The addition of Cel9K to a commercial enzyme set (Celluclast 1.5L + Novozym 188) increased the saccharification of the pretreated reed and rice straw powders by 30.4% and 15.9%, respectively. The results suggest that Cel9K can be used to enhance the enzymatic conversion of lignocellulosic biomass to reducing sugars as an additive.

• Proceedings of the Korea Contents Association Conference
• /
• 2009.05a
• /
• pp.597-601
• /
• 2009

The purpose of this study is to closely examine the effect of exercise behavior change of casino securities on their physical self-description. This observation takes place in casino enterprises in Seoul, Busan, Kangwon and Jeju. Within these areas, we have selected companies with more than 30 securities. Among 450 persons sampling unit, we have excluded 77 copied which seem to be insincere, and actually used 373 copies in this study. Evaluation forms are used as a study method; each form consists of continuance 5 points Likert scales and nominal/proportional scaling and used after excluding a test through the analysis of validity and reliability. After encoding and inputting the framing completed data along with each purpose, it was computerized by computer process, making use of SPSS 15.0 version. Through the data analysis according to these methods and procedures, the result on this study is described below. First, the exercise behavior according to socio-demographic characteristics. Second, the decision-making balance according to socio-demographic characteristics. Third, the self-efficacy according to socio-demographic characteristics. Fourth, the physical self-concept according to socio-demographic characteristics. Fifth, the exercise behavior influence indirectly on the physical self-concept throughout the decision-making balance and self-efficacy.

• Journal of Broadcast Engineering
• /
• v.14 no.1
• /
• pp.59-69
• /
• 2009

A bi-directional symmetric prediction technique has been developed to improve coding efficiency of B-slice and to reduce the computational complexity required to estimate two motion vectors. On the contrary to the conventional bi-directional mode which encodes both forward and backward motion vectors, it only encodes a single forward motion vector, and the missing backward motion vector is derived in a symmetric way from the forward motion vector using temporal distance between forward/backward reference frames to and from the current B picture. Since the backward motion vector is derived from the forward motion vector, it can halve the computational complexity for motion estimation, and also reduces motion vector data to encode. This technique always derives the backward motion vector from the forward motion vector, however, there are cases when the forward motion vector is better to be derived from the backward motion vector especially in scene changes. In this paper, we generalize the idea of the symmetric coding with forward motion vector coding, and propose a new symmetric coding with backward motion vector coding and adaptive selection between the conventional symmetric mode and the proposed symmetric mode based on rate-distortion optimization.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.40 no.6
• /
• pp.84-89
• /
• 2003

In this paper, we real-time implemented to the TMS320C5416 the vocoder of variable bit rate applied the SOLA-B algorithm by Henja to the ITU-T G.729A vocoder of 8kbps transmission rate. This proposed method using the SOLA-B algorithm is that it is reduced the duration of the speech in encoding and is played at the speed of normal by extending the duration of the speech in decoding. At this time, we bandied that the interval of cross correlation function if skipped every 3 sample for decreasing the computational amount of SOLA-B algorithm. The real-time implemented vocoder of C.729A and SOLA-B algorithm is represented the complexity of maximum that is 10.2MIPS in encoder and 2.8MIPS in decoder of 8kbps transmission rate. Also, it is represented the complexity of maximum that is 18.5MIPS in encoder and 13.1MIPS in decoder of 6kbps, it is 18.5MIPS in encoder and 13.1MIPS in decoder of 4kbps. The used memory is about program ROM 9.7kwords, table ROM 4.5kwords, RAM 5.1 kwords. The waveform of output is showed by the result of C simulator and Bit Exact. Also, for evaluation of speech quality of the vocoder of real-time implemented variable bit rate, it is estimated the MOS score of 3.69 in 4kbps.

• Parasites, Hosts and Diseases
• /
• v.37 no.3
• /
• pp.163-169
• /
• 1999

House dust mite allergens have been well established as sensitizing agents that are important in the induction of allergic diseases. In order to analyze epitopes of the allergen and to develop a quantitative method of the allergen exposure, monoclonal antibodies against a recombinant Der p 2 (rDer p 2), one of the major allergens of Dermatophogoides pteronyssinus, were produce. Four monoclonal antibodies produced wee species-specific and did not cross-react to the D. farinae crude extract. Two of the monoclonal antibodies were found to be IgG1 and the others were IgM. For the analysis of epitopes, a Der p 2 cDNA encoding 126 amino acids (aa) was dissected into three fragments with several overlapping peptides, A (aa residues 1-49), B (44-93), and C fragment (84-126). Three monoclonal antibodies showed reactivities to the recombinant B fragment and to the full-length rDer p 2, but one monoclonal antibody reacted only with the full-length rDer p 2. Two-site capture ELISA was developed using two different monoclonal antibodies for quantitating Der p2 in house dust. The sensitivity limit was 4ng/ml with rDer p2 and $8{\;}\mu\textrm{g}/ml$ with the d. pteronyssinus crude extract. The result suggested that the assay using monoclonal antibodies against rDer p2 could be useful for the environmental studies and for the standardization of mite allergen extracts.

• The Korean Journal of Food And Nutrition
• /
• v.15 no.2
• /
• pp.104-111
• /
• 2002

The peptidyl prolyl sis-trans isomerase (PPIase, EC 5.2.1.8) from bacillus stearothermophilus was extracted from the cells treated with by lysozyme. PPIase was purified from the cell extracts by heat treatment, ammonium sulfate precipitation, ion exchange chromatography and finally gel filtration, sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE). The molecular weight of the purified PPIase was estimated as 18kDa by SDS-PAGE. The 39 amino acid residues from the N-terminus were determined by the protein sequencer. The enzyme showed the optimum pH at 8.0 and was stable at the range of pH 7.0∼8.0. The enzyme was considerably stable after heat treatment at 60$\^{C}$ for 30minutes, and the enzyme was quite stable up to 65$\^{C}$. The presence of the PPIase in the refolding solution accelerated the isomerization rate of the assay peptide. PPIase gene of Bacillus stearothermophilus was screened from a genomic library by plaque hybridization using the A-l primer as a probe. A PPIase positive plaque contained a 3.0kb insert of the chromosomal DNA. A 3.0kb fragment was subcloned into pUC18, resulting pPI-40. A DNA fragment encoding the N-terminal portion of the PPIase in pPI-40 was amplified by polymerase chain reaction(PCR) method using the A-1 and B-2 primers. The amplified fragment was cloned into the Sma I site of pUC18 and recombinant plasmid was designated as pSN-18. The nucleotide sequence of 167bp fragment was determined. The deduced amino acid sequence of PPIase was completely matched with the determined N-terminal amino acid sequence of PPIase B. stearothermophilus.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.9
• /
• pp.5011-5016
• /
• 2013

Background: Several epidemiological studies have shown associations between colorectal cancer (CRC) risk and type 2 diabetes and obesity. Any effects would be expected to be mediated through the insulin pathway. Therefore it is possible that variants of genes encoding components of the insulin pathway play roles in CRC susceptibility. In this study, we hypothesized that polymorphisms in the genes involving the insulin pathway are associated with risk of CRC. Materials and Methods: The associations of four single nucleotide polymorphisms (SNPs) in IGF-I (rs6214), IGFBP-3 (rs3110697), INSR (rs1052371), and IRS2 (rs2289046) genes with the risk of CRC were evaluated using a case-control design with 167 CRC cases and 277 controls by the PCR-RFLP method. Results: Overall, we observed no significant difference in genotype and allele frequencies between the cases and controls for the IGF-I, IGFBP-3, INSR, IRS2 gene variants and CRC before or after adjusting for confounders (age, BMI, sex, and smoking status). However, we observed that the IRS2 (rs2289046) GG genotype compared with AA+AG genotypes has a protective effect for CRC in normal weight subjects (p=0.035, OR=0.259, 95%CI= 0.074-0.907). Conclusions: These findings do not support plausible associations between polymorphic variations in IGF-I, IGFBP-3, INSR, IRS2 genes and risk of CRC. However, the evidence for a link between the IRS2 (rs2289046) variant and risk of CRC dependent on the BMI of the subjects, requires confirmation in subsequent studies with greater sample size.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.4
• /
• pp.638-643
• /
• 2007

The full encoding sequence for human type II hexokinase (HXK II) was cloned into the E. coli expression vector pET 21b and expressed as a C-terminally hexahistidine-tagged protein in the BL2l (DE3) strain. The IPTG-induced HXK II approximately accounted for 17% of the total E. coli proteins, and 81% of HXK $II_{6{\times}His}$ existed in inclusion bodies. To improve the production of soluble recombinant HXK II protein, in the functionally active form, we used low temperature, and the osmotic stress expression method. When expressed at $18^{\circ}C$, about 83% of HXK $II_{6{\times}His}$ existed in the soluble fraction, which amounted to a 4.1-fold yield over that expressed at $37^{\circ}C$. The soluble form of HXK $II_{6{\times}His}$ was also highly produced in the presence of 1M sorbitol under the standard condition $(37^{\circ}C)$, which indicated that temperature downshift and low water potentials were required to improve the yield of active recombinant HXK II protein. The expressed protein was purified by metal chelate affinity chromatography performed in an IDA Excellose column charged with $Ni^{2+}$ ions, resulting in about 40mg recombinant HXK II protein obtained with purity over 89% from 51 of E. coli culture. The identity of HXK $II_{6{\times}His}$ was confirmed by Western blotting analysis. Taken together, using the stress-governed expression described in this study, human active HXK II can be purified in sufficient amounts for biochemical and biomedical studies.