• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.251-255
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• 2010

This study was conducted to investigate the detrimental effects of decapping and emasculation on the postharvest quality and the vase life of cut Cymbidium flowers with or without 1-Methylcyclopropene (1-MCP) and Aminoethoxyvinylglycine (AVG) treatments. The vase life treated by 1-MCP 150 ppb for four hours regardless of decapping or emasculation was significantly prolonged 12 days more. Ethylene production in the treatment without 1-MCP began to be raised from 7days, 1-MCP treatments, regardless of the concentration of processing did not occur until 15days. Ethylene began to production when the lip began to coloration. Compared to the 1-MCP effect, the AVG treatment of 0.5 and 1mM for four hours prolonged the vase life about nine days more. Whereas, the only decapping or emasculation treatments were effective to more three days longer in the vase life of cut Cymbidium' Halleluiah' flowers. 1-MCP treatment was very beneficial for Cymbidium 'Halleluiah' cut flowers than AVG.

• The Journal of Natural Sciences
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• v.11 no.1
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• pp.99-103
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• 1999

This study was carried out to use as the basic data for breeding by evaluating parthenocarpy with 12 tomato lines and 17 allied species. Fruit set of open pollination plots was over 90% in 11 lines(CLN430-85-13-5 etc), 9.1-50% in 10 lines(LA1306 etc.), and the rest no fruit set. Fruit set after emasculation resulted highly 51.4% in CLN431-85-13-12, 53.9% in CLN425-130-8-1, 66.7% in CLN435-185-4-9, and 72.2% in Ventura, respectively. But fruit set in other tomato lines resulted under 50% and 17 allied species(LA1306 etc) resulted no effect of fruit set. Fruits other emasculation had no seeds and fruits after open pollination had 10-70 seeds per fruit. This result of this work showed that 3 lines, CLN435-185-4-9, CLN425-130-8-1, and CLN431-85-13-12, resulted in good effectiveness on the evaluation of parthenocarpy in tomatoes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.24 no.4
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• pp.38-44
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• 1979

$^14$C-sucrose was labeled on detached panicles and $^14{CO}_2$ on flag leaves or panicles of intact plants to study grain sink activity in spring oats cultivar Pennfield. Defoliation and emasculation experiment was conducted to study source-sink relationship during grain filling. Specific activity of groat rose up to 15 days after anthesis and declined rapidly to 18 days. Daily gain of groat wt. matched closely with specific activity of. groat during grain filling. Primary groats were higher in specific activity of groat than secondary groats.$^14{CO}_2$ exposure on panicle was three times higher in specific activity of groat than $^14{CO}_2$exposure on flag leaf. In the defoliation and emasculation experiment, groat wt. of Pennfield oats decreased as ratio of source/sink decreased. Half number of spikelets with half leaf area was no different in groat wt. compared to control, but normal number of spikelets with zero leaf area was decreased 16% in groat wt., indicating a significant compensation by green area on panicle.

• Archives of Reconstructive Microsurgery
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• v.10 no.1
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• pp.23-27
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• 2001

Penile amputation results primarily from attempts at self-emasculation in the psychotic individual or from felonious assault. Because of the complex nature of patients whom perform self-emasculation and low incidence of felonious assault, large series of these individuals do not exist, and experience with amputation injuries of the genitals is confined to individual case reports. Fortunately, complete amputation of penis is quite rare but potentially devastating occurrence. The repels of the treatment of penile amputation by coporal reapproximation that have been published since 1977 indicate that microreplantation techniques are propably superior to the technically simpler nonmicroscopic coporal reapproximation techniques. As microsurgical techniques have been demonstrated to superior, the preferred method of replantation involves this routine whenever possible. We have experience of a case of successful replantation of completely amputated penis by using microneurovascular repair, with the good result of cosmetic and functional concern. Herein, we report this case with the review of the literatures.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.496-501
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• 2009

In order to promote the efficiency of strawberry breeding programs, pollen viability of strawberry, 'Seolhyang' was investigated using the fluorochromatic reaction test and seed set under controlled environment. Pistil receptivity was also assessed by hand pollination. Four varieties including 'Maehyang' were used for the test of pistil receptivity with 'Seolhyang' as a pollen parent. Pollen viability remained high for several days under dry conditions as below 33% relative humidity while the greatest loss of viability occurred at 76% relative humidity. The viability was rapidly decreased at high humidity and almost all grains were unviable in 7 days after storage. Pollen viability does not appear to be drastically reduced if the relative humidity is low. Therefore, humidity is more important factor than temperature for the pollen viability in Fragaria${\times}$ananassa. The rate of seed set by hand pollination lasted higher than the average of 77.2% from 2 to 8 days after emasculation when the daily average temperature was around $15^{\circ}C$ in plastic house. It began to decline gradually from 10 days and had decreased dramatically after 12 days except several cultivars. Based on the daily mean accumulated temperature, it is recommended to have an artificial pollination between the range of $45{\sim}140^{\circ}C$ after the emasculation to increase the rate of seed set in strawberry.

• Korean Journal of Medicinal Crop Science
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• v.28 no.4
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• pp.298-309
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• 2020

Background: Safflower (Carthamus tinctorius L.) is a useful medicinal and oil crop in Korea. However, when safflower is cultivated, the flowering period overlaps with the rainy season, and seed maturation is poor. Therefore, this study aimed to use basic research data to develop superior varieties using agronomic characteristics and crossing method. Methods and Results: A total of 34 safflower germplasms were sown and their agronomic characteristics were investigated. Based on these investigations, the cultivar 'ui-san-hong-hwa' was selected as the mother plant, and 'Myanmar safflower' (Hsu Pan) was selected as the father plant. In addition, we developed a floret-protecting cap to cover florets after emasculation during the artificial crossing. When florets were protected by the cap, the seed setting rate increased in comparison to that in the non-covered florets. Conclusions: Agronomic characteristics can contribute to developing suitable varieties. The results suggest that the protection cap will be helpful in breeding without the floral organ drying. This study contributes an efficient breeding method to develop new safflower varieties.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.420-424
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• 2006

This experiment was conducted to elucidate the basic physiological phenomena of pollination and fertilization for breeding in perilla (Perilla frutescens) through different 6 varieties derived from 2 genera under 4 temperature conditions at day and night. The pollen germination was observed after 30 minutes and pollen tube reached the lower part of pistil after 1 hour from artificial pollination. Seed formation was affected by temperature condition and a variety. The early maturing variety 'YCPL 25' showed a poor seed formation rate under the night temperature below $15^{\circ}C$, but the late maturing variety 'YCPL263' did not lowered even though the night time temperature was $10^{\circ}C$. The function of pistil was long maintained under the low temperature from the fact that if the live pollen pollinate artificially to the flower that does not form seed under low temperature, fruiting was made. Pollen was created and pollen tube was developed in the time of petal becomes bigger than calyx in five varieties, 'YCPLl77-1' etc. However, pollen was made and pollen tube was made only 71% in a green chajogi 'YCPL205-1'. These physiological phenomena of pollination and fertilization could be applicable to the emasculation and an effective breeding in perilla.

• Journal of Plant Biotechnology
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• v.33 no.1
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• pp.27-32
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• 2006

To clarify the roles of bromelain in plants, we isolated BL1 gene encoding bromelain from pineapple stem tissues and sequenced. The full length cDNA is 933 bp and encodes a polypeptide of 311 amino acid residues. The cDNA is most similar 94% at the amino acid level to bromelain previously isolated from pineapple (BAA21929). Explants of Lactuca sativa were co-cultivated with Agrobacterium tume-faciences LBA 4404 strains containing nptII and BL1 gene for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 Ms basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were obtained T1 generation seeds with emasculation, and tested with PCR analysis using 35S promoter and BL1 specific primers whether BL1 gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot and endo protease activity showed that transcripts of BL1 gene were detected in transgenic lines. Theses results suggest that BL1 gene be successfully integrated and transcripted in the transgenic lettuce plants.

• Journal of Korean Society of Forest Science
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• v.94 no.3 s.160
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• pp.178-182
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• 2005

To identify the seedlings from controlled pollination between one paternal tree and three maternal trees of Japanese red pine, cpSSR markers of the paternally inherited haploid genome were analyzed in two year old 114 seedlings of full sib families. Individual specific DNA fingerprint like haplotypes of the parental trees were determined by PCR with three cpSSR primers. Haplotypes of the 114 seedlings were also identified by PCR with the same primers. On the basis of the comparison of cpDNA haplotypes of the 114 seedlings with those of the parental trees, 14 seedlings revealed to have distinguished haplotypes from those of the paternal tree. It was tentatively concluded that they were generated via pollination with the non-paternal trees. A seedling of Gangwon30 revealing non-paternal haplotype might have been generated via self pollination with the pollens of maternal tree through improper emasculation or contamination during artificial pollination. DNA fingerprint like cpSSR profiles observed in this study could be successfully applied to the various plant forensic analyses, such as identification of siblings of individual trees, asexually reproduced ramets of a specific clone, vegetatively propagated individuals via tissue culture, and pure full sib progenies.