• Title/Summary/Keyword: electronmicroscopy

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Clinical Signs and Pathologic Lesions of Foot and Mouth Disease in Pigs, Korea (국내 발생 돼지 구제역의 임상증상, 육안병변 및 현미경적 병변)

  • 배유찬;윤순식;강경일;노인순;김희진;소병재;박중원;진영화;강문일
    • Journal of Veterinary Clinics
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    • v.21 no.2
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    • pp.172-176
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    • 2004
  • We described the clinical signs, gross lesions, histopathological lesions, and electronmicroscopy of pig Foot-and-Mouth Disease cases which had occurred in 2002, Korea. Grossly affected pigs showed vesicles on snout, tongue, coronary band, and udder. Histoapthologically, severe intercellular edema and necrosis of prickle cells, and separation between epidermis and dermis were found on the mucosa of tongue and coronary band. And myocardial necrosis and mononuclear cells infiltration in myocardium were found. Electronmicroscopically numerous picornavirus particles(18∼22 nm in diameter) were found in the cytoplasm of prickle cells. By those results, we confirm that those cases were typical FMD cases.

Bovine papillomavirus detection from bovine teats using immunohistochemistry and electronmicroscopy (면역조직화학염색 및 전자현미경검사를 이용한 소 유두로부터 소유두종바이러스 검출)

  • Bae, You-Chan;Yoon, Soon-Seek;Park, Jung-Won;Lee, Cheong-San;Jean, Yong-Hwa;Kang, Mun-Il
    • Korean Journal of Veterinary Research
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    • v.45 no.2
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    • pp.233-238
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    • 2005
  • We examined the teats of slaughtered cattles to investigate the prevalence of papilloma and to detect bovine papilloma virus (BPV) from those samples. Among 880 heads, 432 (49.0%) was Holstein and 448 (51.0%) was Korean native cattle. Grossly, out of 432 heads (Holstein), 263 (60.8%) had papilloma lesions on teats. However, out of 448 heads (Korean native cattle), only 33 (7.4%) had papilloma lesions on teats. Immunohistochemically, BPV antigen was detected in 35 heads (22.9%) of 153 (Holstein) which had papilloma lesions. But, in Korean native cattle, BPV antigen was detected in 1 head (3.8%) of 26. Electronmicroscopically, in Holstein papilloma cases, BPV particles was detected in 31 heads (39.2%) of 79. This study revealed that papilloma was very prevalent in holstein teats, Korea and the prevalence of papilloma in Holstein was 8 times higher than that of Korean native cattle. Moreover, it was needed to consider gross and histopathological lesions to confirm BPV infection on teats because the sensitivity of immunohistochemistry and electronmicroscopy was not high.

Localization of worm antigen in Neodiplostomum seoulense by immuno-electronmicroscopy (면역전자현미경법으로 관찰한 서울주걱흡충에서 충체 항원의 분포)

  • LEE, Jae-Chul;KONG, Yoon;LEE, Soo-Ung;HUH, Sun
    • Parasites, Hosts and Diseases
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    • v.35 no.2
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    • pp.95-104
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    • 1997
  • The localization of worm antigen of Neodiplostomum seoulere was examined by immuno-electronmicroscopic observation. Not only the immunized serum of mice with crude worm extract of N. seoulene but also serum of infected mouse were reacted to the worm section. Using immunized serum as primary antibody. the gold particles were deposited on the rough endoplasmic reticulum of the cell of tribocytic organ, spermatozoa in the seminal vesicle, microvilli of the caecum and vitelline follicle. Using infected serum, gold particles were deposited only on the vitelline follicle prominently. This finding suggested that the tribocytic organ, seminal vesicle, caeca and vitelline follicles may play a role of antigen to immunized serum with crude worm extract of N. seoulense, whereas the vitelline follicle, to the infected serum.

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Isolation and Identification of Porcine Rotavirus from Piglets with Diarrhoea (하리자돈(下痢子豚) 분변(糞便)에서 Rotavirus 분리(分離) 동정(同定))

  • Kim, Hee-sun;Choi, Chung-ok;Kim, Doo-hee;Lyoo, Young-soo;An, Soo-hwan
    • Korean Journal of Veterinary Research
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    • v.28 no.1
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    • pp.89-97
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    • 1988
  • Twenty-eight porcine rotavirus were isolated from piglets with diarrhoea in chonnam province. According to the age, 41 to 60 day old pigs showed the highest isolation frequency among the post weaning pigs. The characteristics of the field isolates were determined by electronmicroscopy(EM), immunofluorescent assay(FA), and electrophoretic migration patterns of the genome profiles. Some of the isolates showed remarkable haemagglutination activity against rabbit and dog erythrocytes, ranged from 4 to 2848, respectively. At least 3 serotypes of porcine rotavirus were recognized by serum neutralization test using serotype specific rotavirus hyperimmune sera.

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Preparation and Characterization of Small Sized PZT Powders: A Sol-Gel Modified Approach

  • Choi, Alka Yong-Woo;Kwon, Oh-H.;Choi, Kyu-M.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.11a
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    • pp.245-246
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    • 2006
  • A propyl alcohol based sol-gel method was used as to replace the 2-methoxyethanol with 1,1,1 tris (hydroxymethyl) ethane for preparation of pzt piezoelectric ceramic. The powder obtained by this sol-gel process were calcined at $900^{\circ}C$ and followed by the sintering at $1100^{\circ}C$ for ca2 hrs as to reach a pyrochlore-free crystal phase. The characterization of synthesized material was carried out by the XRD analysis and the surface morphology were determined by high resolution scanning electronmicroscopy. Further, the prepared small sized pzt thin powders are likely to be used m various applications.

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A study on noise properties of Co films deposited on Si (실리콘 기판에 증착된 코발트 박막의 잡음특성 연구)

  • 조남인;유순재
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.33A no.2
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    • pp.122-130
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    • 1996
  • In an effort to learn more about the reaction mechanisms which lead to the compound nucleation at the interface of cobalt and silicon, electrical noise properties has been investigated for cobalt thin films deposited on silicon substrates by the electron beam evaporation and rf sputtering techniques. Microstructural variations at the Co/Si interfaces have been observed by transmission electronmicroscopy. Amorphous structures are observed at the Co/Si interfaces for samples whose cobalt thicknesses are less than 4nm and a polycrystalline compound nucleation has been occurred for thicker films. 1/f noise power same samples, and the spetral density has been normalized. The amplitude of 1/f noise power spectral density shows a gradual increase as the cobalt thickness is increased, and the amplitude has dropped abruptly after the compound nucleation. The variations of the noise parameters areassumed to be an indiction of the phase transformation along the nucleation reaction path, and amplitude has been interpreted as instabilities of the Co/Si interfacial structures.

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Studies on streptomycin resistant mutant strains of rhizobium trifolii (Rhizobium trifolii의 스트렙토마이신 내성 돌연변이주의 특성)

  • 신종희;허연주;이영록
    • Korean Journal of Microbiology
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    • v.25 no.4
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    • pp.290-296
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    • 1987
  • Some streptomycin resistant strains of Rhizobium trifolii having nodulation ability were selected, and their nitrogenase activities, symbiotic effects on plant growth, and nodule electronmicroscope were compared with those of the wild type. After NTG treatment, as a mutagen, at the concentration exhibiting 99.7% lethal rate, 5 strains of streptomycin resistant mutant having nodulating ability were selected. Among these nodulating mutant strains, 3 strains produced more nodules and 2 strains showed less nodules than wild type. But their nitrogenase activities were decreased significantly, and nodule formation time was also delay compared with those of the wild type, and there was no remarkable difference in effects on plant growth. Microstructure of nodules by electronmicroscopy had mant distinctive differences between red clover nodules inoculated with wild type and mutants.

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The Utilization of Waste Seashell for High Temperature Desulfurization

  • Kim, Young-Sik;Hong, Sung-Chul
    • Journal of Environmental Health Sciences
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    • v.36 no.2
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    • pp.136-140
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    • 2010
  • The integrated gasification combined cycle (IGCC) is one of the most promising proposed processes for advanced electric power generation that is likely to replace conventional coal combustion. This emerging technology will not only improve considerably the thermal efficiency but also reduce or eliminate the environmentally adverse effects normally associated with coal combustion. The IGCC process gasifies coal under reducing conditions with essentially all the sulfur existing in the form of hydrogen sulfide ($H_2S$) in the product fuel gas. The need to remove $H_2S$ from coal derived fuel gases is a significant concern which stems from stringent government regulations and also, from a technical point of view and a need to protect turbines from corrosion. The waste seashells were used for the removal of hydrogen sulfide from a hot gas stream. The sulphidation of waste seashells with $H_2S$ was studied in a thermogravimetric analyzer at temperature between $600^{\circ}C$ and $800^{\circ}C$. The desulfurization performance of the waste seashell sorbents was experimentally tested in a fixed bed reactor system. Sulfidation experiments performed under reaction conditions similar to those at the exit of a coal gasifier showed that preparation procedure and technique, the type and the amount of seashell, and the size of the seashell affects the $H_2S$ removal capacity of the sorbents. The pore structure of fresh and sulfided seashell sorbents was analyzed using mercury porosimetry, nitrogen adsorption, and scanning electronmicroscopy.

Influence of Y-Doped on Structural and Optical Properties of ZnO Thin Films Prepared by Sol-Gel Spin-Coating Method

  • Park, Hyunggil;Leem, Jae-Young
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.336-336
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    • 2013
  • Zinc oxide (ZnO) based transparent oxide semiconductors have been studied due to their high transmittance and electrical conductivity. Pure ZnO have unstable optical and electrical properties at high temperatures but doped ZnO thin films can have stable optical and electrical properties. In this paper, transparent oxide semiconductors of Y-doped ZnO thin films prepared by sol-gel method. The ionic radius of $Y^{3+}$ (0.90 A) is close to that of $Zn^{2+}$ (0.74 A), which makes Y suitable dopant for ZnO thin films. The Sn-doped ZnO thin films were deposited onto quartz substrates with different atomic percentages of dopant which were Y/Zn = 0, 1, 2, 3, 4, and 5 at.%. These thin films were pre-heated at $150^{\circ}C$ for 10 min and then annealed at $500^{\circ}C$ or 1 h. The structural and optical properties of the Y-doped ZnO thin films were investigated using field-emission scanning electronmicroscopy (FE-SEM), X-ray diffraction (XRD), UV-visible spectroscopy, and photoluminescence (PL).

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Enterotoxemia caused by Clostridium perfringens type A in Formosan deer (꽃사슴의 Clostridium perfringens A형에 의한 장독혈증 발생 보고)

  • 이청산;한성태;곽학구;박경재;현공율;조우영;이종인;배유찬;진영화
    • Korean Journal of Veterinary Service
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    • v.25 no.2
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    • pp.127-133
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    • 2002
  • The case reports for clostridium type A enterotoxemia in Formosan deer have rarely been reported. This paper describes a natural case of type A enterotoxemia in farmed Formosan deer in Cheongwon-gun. A dead, male 10-month-old Formosan deer was submitted to Chungbuk Livestock and Veterinary Research Institute, March 24, 2001 and examined. That deer was fed with assorted grain feed, oak leaves, acorn and bean curd. Grossly there was no visible external change. Despite of the carcass being examined within 12 hours of death, there was a quite degree of posonortem decomposition. There was severe hemorrhage in the serosa of abomasum and small intestine. Much blood tinged and watery contents were contained in those organs. Also there were severe swelling of spleen, some red foci in hepatic parenchyma. Microscopically there were severe congestion and hemorrhage in mucosa submucosa, muscular layer, and serosa of abomasum and small intestine. Also spleen and pancreas showed severe Congestion and hemorrhage. There were multifocal hemorrhage with hepatic necrosis in periportal area and focal mononuclear cell deposition in sinusoid. In bacterial culture for small intestine, Cl perfringens was isolated. By toxin typing for the strain, that had $\alpha$ -toxin belonged to type A. In electronmicroscopy for feces, no vims particle was detected. Considering clinical signs, gross lesions, microscopic lesions, bacterial culture, and toxin typing of the isolate, this case was diagnosed as enterotoxemia by Cl perfringens type A.