• Title/Summary/Keyword: electric field strength

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High Frequency Electroporation-transformation of Coryneform Bacteria Grown in the Medium with Penicillin-G (Penicillin-G 첨가 배지에서 배양한 코리네형 세균의 전기장 충격법에 의한 고효율 형질전환)

  • 노갑수;김성준
    • KSBB Journal
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    • v.6 no.3
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    • pp.223-230
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    • 1991
  • Using the shuttle vector pECCGl between Escherichia coli and Corynebacterium glutamicum and C. glutamicum strain JS231 grown in the medium supplemented with penicillin-G, which inhibits the formation of cross-links in the peptidoglycan of bacterial cell wall, various parameters involved in electroporation system including resistance, electric field strength, capacitance, DNA concentration, and cell density were investigated independently and optimized for the high efficiency transformation of coryneform bacteria. Using cells grown with 0.3U/ml of penicillin-G and harvested at A600 of 0.7-0.8, transformation efficiencies of 107-l08 transformants/$\mu\textrm{g}$ of DNA with Corynebcctertum glutamicum strain JS231 and wild type ATCC13032 were achieved under conditions of 12.5kV/cm of electric field strength, 400 ohms of resistance, $25\mu$F of capacitance, 3$\times$108 cells per transformation(1.2$\times$1010 cells/ml) and 100ng of plasmid DNA per transformation.

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Performance Test of 3 Port ERF Valve for Controlling Flow Rate-Direction (3 포트 ERF 밸브의 유량-방향 제어 성능 실험 -유압시스템 및 자동화 융합연구-)

  • Jang, S.C.
    • Journal of Power System Engineering
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    • v.16 no.6
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    • pp.92-97
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    • 2012
  • In this study, ER fluids were working fluid and yield shear stress of attained dispersive ER fluids made by analyzed to adapt effectively at the practical equipment like three port ER valve. In addition, movement of ER particle was observed by using a microscope the same as the strength of electric field with Bingham characteristic. Basis material was presented to develop 3 port ER-Valve by quantitatively comparing and analyzing entrance, load and outlet flow's differences of 3 port rectangular tubes. This study, after designing and producing the 3port ER-Valve, considered the characteristic of pressure drop, outlet and load flow rate by changing the strength of electric field on ER fluids flowing between electrodes.

The Measurement and Analysis The Electromagnetic Wave by Discharge in Air for Insulation Diagnosis (절연진단을 위한 기중방전에서의 방사전자파 측정과 분석)

  • 이상훈;이현동;이춘하;윤영대;김기채;이광식;이동인
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 1999.11a
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    • pp.141-144
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    • 1999
  • This paper offer fundamental ones about insulation diagnosis with electromagnetic wave, when discharges occurred. To simulate Partial-Discharge(PD), It is used needle-plane electrode in air. As the voltages applied, measure the electromagnetic wave with monopole antenna and analysis its fundamental characteristics, then, acquired data were operated. The results presented as the form of graph, and the magnitude of charge when occurred PD simultaneously. In conclusion, when the magnitude of charge over 100[pC], the electric field strength changed, and then the magnitude of charge and the electric field strength increased samely.

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Insulated Characteristics of PET Film under $SF_6$ ($SF_6$ 가스중 PET Film의 절연특성)

  • Kim, J.D.;Jung, J.G.;Joo, S.C.;Lee, D.J.;Park, J.Y.;Lee, Y.G.
    • Proceedings of the KIEE Conference
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    • 1999.07e
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    • pp.2120-2122
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    • 1999
  • $SF_6$ gas has been widely used the gas insulated transmission line, gas insulated transformer and gas insulated substation and Polyester film used as the turn insulation with other material. $SF_6$ gas has been actively studied in domestic and foreign countries. In general, when dielectric surface electric field exceed any electric field strength on air, corona discharge is occurred from the dielectric surface. This paper presents the insulation strength of turn insulation of $SF_6$-PET film studied under application of AC voltage, constitute of the plane to plane with PET Film. The results obtained in experimental are summarized that Partial Discharge Initial Voltage(PDIV) of turn insulation depend on gas pressure, but Breakdown Voltage(BDV) did not depend on gas pressure.

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A study on the electric field strength of the insulation cover using 22.9 kV-y distribution line (22.9 KV-y 배전선로용 전선보호관 전계강도 비교실험 연구)

  • Chon, Y.K.;Seo, K.S.;Cha, K.S.
    • Proceedings of the KIEE Conference
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    • 2000.07c
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    • pp.1871-1873
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    • 2000
  • There are many electrical accidents between bare wire and line post insulator which is degraded by long time using, causing leakage current on the surface of insulator. In this paper it is presented that the 22.9 Kv-y distribution lines are protected by binding cover which is needed from fog or rainy moisture, surges occurring by switching or lightening pulse. It is analyzed to investigate the unbalanced transfer characteristics and reflection coefficient by using binding cover and non-cover. It was tested the radio interference voltage in the test room and electric field strength by calculation between binding cover and non-cover. In the factory the leakage current causing on the surface of insulator was tested in the state of light load and heavy load.

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A Numerical Study on the Collection Characteristics of a Wide Plate-Spacing Electrostatic Precipitator (광폭 전기집진기의 집진 특성에 관한 수치적 연구)

  • 김용진;홍원석;정상현;하병길;하대홍;유주식
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.13 no.12
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    • pp.1205-1213
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    • 2001
  • The electrical characteristics and particle collection efficiency of a wide-plate spacing electrostatic precipitator are numerically investigated, and the results are compared with those obtained experimentally. The electric potential and field strength near the collection plate increase with increasing the plate spacing. The electric field strength of a discharge electrode of a twisted pin type is larger than that of a rectangular type. As the roughness factor of the discharge electrode wire becomes small, or the plate spacing becomes narrow, the corona current of the precipitator increases. The numerical results agree well with those obtained from experimental method.

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Analysis of Small Current Interruption Performance for $SF_6$ Gas Circuit Breaker ($SF_6$ 가스차단기의 소전류 차단성능 해석)

  • Kim, Hong-Kyu;Song, Ki-Dong;Chong, Jin-Kyo;Oh, Yeon-Ho;Park, Kyong-Yop
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.55 no.10
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    • pp.528-533
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    • 2006
  • To analyze the small current interruption performance for the gas circuit breakers, the gas density and electric field intensity should be calculated. In this paper, the FVFLIC method is used for the gas flow analysis and the FEM for the electric field analysis. Then, the dielectric withstanding voltage is evaluated by the empirical formulation or Streamer theory. By comparing the calculated dielectric strength with the test result, it is found that both methods show good prediction capability for the small current interruption performance. Especially, when both methods predict the same interrupting performance, the prediction is in accordance with the experimental result.

A Study on the Electric Field Strength of Two Bushings for 17.5 kV Recloser (17.5 kV Recloser용 Bushing 2종에 대한 전계강도 연구)

  • Shin, Y.J.;Oh, Y.H.;Kim, M.K.;Lee, J.G.;Kim, S.J.;Kim, J.K.;Kim, K.S.;Jung, J.H.
    • Proceedings of the KIEE Conference
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    • 2003.11a
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    • pp.392-395
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    • 2003
  • The study of electric field analyses has been conducted on two kinds of bushings for 17.5kV $SF_6$ gas insulated recloser. In this paper, two model bushings and input conditions for the analyses are described in detail. The results of the electric field analyses are presented and compared with to find out the better design in two bushings. Finally some suggestions are given for improving the insulation characteristics of the bushings.

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A Study on the Technical Regulation of Weak Electric Filed Strength Radio Equipment about 8.2Hz Frequency Band (8.2MHz 대역 미약 전계강도 무선기기의 출력기준에 관한 연구)

  • Kim, Sun-Youb;Ra, Yoo-Chan
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.13 no.12
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    • pp.2498-2504
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    • 2009
  • This paper compared the output limit value of the Korean weak electric field strength wireless device in the 8.2MHz band with the standard values of foreign countries. Through this, the study confirmed that the Korean regulation was lower by about 10-20dB than those of the USA or Europe. In order to prove this, the study measured outputs by entrusting the 8.2MHz EAS system to two measuring companies. As a result of these measurements, electric field strengths were shown to be $70.6dB{\mu}V/m$ and $68.3dB{\mu}V/m$ respectively, and these values were confirmed to exceed the current Korean standard of $59.8dB{\mu}V/m$. Accordingly, it is deemed necessary to review the specifications of the Korean standard in the 8.2MHz band.

A Study on the Technical Regulation of Weak Electric Filed Strength Radio Equipment about 58kHz Frequency Band (58kHz 대역 미약 전계강도 무선기기 기술 기준에 관한 연구)

  • Park, Hyoung-Keun;Kim, Sun-Youb;Ra, Yoo-Chan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.9
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    • pp.2319-2325
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    • 2009
  • This paper compared the output limits value of the Korean weak electric field strength wireless device in the 58kHz band with the standard values of foreign countries. Through this, the study confirmed that the Korean regulation was lower by about 50dB than those of the USA or Europe. In order to prove this, the study measured outputs by entrusting the 58kHz EAS system to two measuring companies. As a result of these measurements, electric field strengths were shown to be $112dB{\mu}V/m$ and $08dB{\mu}V/m$ respectively, and these values were confirmed to exceed the current Korean standard of $102.7dB{\mu}V/m$. Accordingly, it is deemed necessary to review the specifications of the Korean standard in the 58kHz band.