• 로봇학회논문지
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• 제16권3호
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• pp.207-215
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• 2021

There is a risk of serious injury to workers who work at height in pier construction process. By using auto climbing formwork system that does not need to dismantle and reinstall formworks, it is possible to improve work efficiency and safety of workers. However, auto climbing formwork system still requires workers to work on a pier for rebar connection works and so on. In order to eliminate works by workers on the pier, robot manipulators with special end effectors are proposed. Through analysis of works on the pier, three specialized end effectors which are a gripper, a rebar coupler press, and a concrete vibrator, are suggested. Also, new pier construction scenario by the suggested system is confirmed using 3d modeling. It is expected that the proposed system and method enables pier construction without workers on piers. It will increase safety and efficiency of pier construction.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.1651-1656
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• 2005

This paper describes a natural frequency analysis conducted to find out a suitable working area for a spring-manipulator system generating a large vibrating force with mechanical resonance. Large force generation is one of the functions that we hope for a robot. For example, a weeding robot is required to generate a large force, because some weeds have roots spreading deeply and tightly. The spring-manipulator system has a spring element as an end-effector, so it can be in a state of resonance with the elasticity of the spring element and the inertial characteristics of the manipulator. A force generation method utilizing the mechanical resonance has potential to produce a large force that cannot be realized by a static method. A method for calculating a natural frequency of a spring-manipulator system with the generalized inertia tensor is proposed. Then the suitable working area for the spring-manipulator system is identified based on a natural frequency analysis. If a spring-manipulator system operates in the suitable working area, it can sustain mechanical resonance and generate a large vibrating force. Moreover, it is shown that adding a mass at the tip of the manipulator expands the suitable working area.

• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.155-160
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• 2009

Mast cells and basophils perform important functions as pivotal effector cells in IgE-mediated allergic reactions. KU812F cells, a human basophilic cell line isolated originally from chronic myelocytic leukemia, express a high affinity receptor of IgE, $Fc{\varepsilon}RI$. Kaempferol was extracted and isolated from a methanolic extract of flavonoid-rich Nelumbo nucifera stamens. In the present study, the inhibitory effects of kaempferol on $Fc{\varepsilon}RI$ expression in human basophilic KU812F cells was examined. Flow cytometric analysis revealed that $Fc{\varepsilon}RI$ expression on the cell surface was suppressed in a concentration-dependent manner when the cells were cultured with kaempferol. Moreover, RT-PCR analysis showed that the mRNA levels for $Fc{\varepsilon}RI$ ${\alpha}-\;and\;{\gamma}$-chains were reduced as the result of kaempferol treatment in a concentration-dependent manner. Kaempferol showed its suppressive effects on intracellular $Ca^{2+}$ concentration and histamine release from anti-$Fc{\varepsilon}RI$ ${\alpha}$-chain antibody-stimulated cells in a concentration-dependent manner. These observations indicate that kaempferol may exert antiallergic effect via down regulation of $Fc{\varepsilon}RI$ expression and degranulation.

• BMB Reports
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• 제48권11호
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• pp.597-598
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• 2015

Mitochondrial respiratory defect is a key bioenergetics feature of hepatocellular carcinoma (HCC) cells. However, their involvement and roles in HCC development and progression remain unclear. Recently, we identified 10 common mitochondrial defect (CMD) signature genes that may be induced by retrograde signaling-mediated transcriptional reprogramming in response to HCC mitochondrial defects. HCC patients with enriched expression of these genes had poor prognostic outcomes, such as shorter periods of overall survival and recurrence-free survival. Nuclear protein 1 (NUPR1), a key transcription regulator, was up-regulated by Ca⁺⁺-mediated retrograde signaling. NUPR1-centric network analysis and a biochemical promoter-binding assay demonstrated that granulin (GRN) is a key downstream effector of NUPR1 for the regulation of HCC cell invasiveness; association analysis of the NUPR1-GRN pathway supported this conclusion. Mitochondrial respiratory defects and retrograde signaling thus play pivotal roles in HCC progression, highlighting the potential of the NUPR1-GRN axis as a novel diagnostic marker and therapeutic target for HCC.

• 대한기계학회논문집A
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• 제21권12호
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• pp.2146-2155
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• 1997

This paper presents the Wire Parallel Mechanism for robot pose measurement which can be used to robot calibration. It is constructed with six parallel links using wire. The position and orientation of the end effector of a robot are calculated from the wire length that measured by the encoder. The unique solution is obtained from a Newton-Raphson method and geometric configuration of the mechanism, also the method to estimate a measuring space is presented. Through the simulations, it is verified that the proposed mechanism can measure a robot pose, and has a large measuring space. In conclusion, it can be used effectively in a robot pose measurement with little cost and effort.

• 대한한방내과학회지
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• 제24권1호
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• pp.134-143
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• 2003

Objectives : We performed this study to understand the molecular basis of the antitumor effect of Saussurea lappa, Pharbitis nil, Plantago asiatica and Taraxacum mongolicum, which have been used for cancer treatment in Korean traditional medicine. Design: We analyzed, the effect of these medicinal herbs on proliferation and apoptosis of tumor cells and its association with gene expression, We performed semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) analysis of cell cycle- and apoptosis-related genes using a gastric cancer cell line AGS. Results : Cell counting assay and $[^3H]thymidine$ uptake analysis showed that Saussurea lappa and Pharbitis nil strongly inhibit cell proliferation of AGS in a dose-dependent manner. Interestingly, gene espression assay revealed that mRNA espression levels of c-Jun, c-Fos, c-Myc, and Cyclin D1 were markedly decreased by Saussurea lappa and Pharbitis nil. Furthermore, Saussurea lappa was identified to activate expression of the p53 tumor suppressor and its downstream effector $p21^{Wafl}$, which leads to $G_1$ cell cycle arrest and apoptosis. These observations suggest that the anticancer effect of Saussurea lappa and Pharbitis nil might be associated with their regulatory capability of tumor-related gene expression.

• 대한건축학회논문집:구조계
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• 제34권4호
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• pp.65-74
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• 2018

The primary objective of this study is to develop a conceptual design of automated pothole patching machine that improves the conventional work in safety, quality, and productivity. For this, the following research works are conducted sequentially; 1)literature review, 2)selection of element technology for conceptual design, 3)deduction of work process and conceptual design, 4)life cycle cost analysis of the conceptual design. As a result, X-Y table telescopic manipulator, pothole patching end effector, realtime pothole recognizer, 3D pothole volume profiler, automated pothole patching machine controller are selected as core technologies. Furthermore, a conceptual design and working process of an automated pothole patching machine are developed based on the core technologies. According to the life cycle cost analysis result, the cost of the automated method was 38.3% less than that of the conventional method, and the economic efficiency was also superior(ROR 77.1%, Break-even Point 23.8month). It is expected that the application range and impact on the construction industry will be enormous due to the increasing trend of road maintenance market.

• Journal of Microbiology and Biotechnology
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• 제21권2호
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• pp.162-169
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• 2011

A dynamic model of lactic acid fermentation using Lactococcus lactis was constructed, and a metabolic flux analysis (MFA) and metabolic control analysis (MCA) were performed to reveal an intensive metabolic understanding of lactic acid bacteria (LAB). The parameter estimation was conducted with COPASI software to construct a more accurate metabolic model. The experimental data used in the parameter estimation were obtained from an LC-MS/MS analysis and time-course simulation study. The MFA results were a reasonable explanation of the experimental data. Through the parameter estimation, the metabolic system of lactic acid bacteria can be thoroughly understood through comparisons with the original parameters. The coefficients derived from the MCA indicated that the reaction rate of L-lactate dehydrogenase was activated by fructose 1,6-bisphosphate and pyruvate, and pyruvate appeared to be a stronger activator of L-lactate dehydrogenase than fructose 1,6-bisphosphate. Additionally, pyruvate acted as an inhibitor to pyruvate kinase and the phosphotransferase system. Glucose 6-phosphate and phosphoenolpyruvate showed activation effects on pyruvate kinase. Hexose transporter was the strongest effector on the flux through L-lactate dehydrogenase. The concentration control coefficient (CCC) showed similar results to the flux control coefficient (FCC).

• 한국패류학회지
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• 제27권3호
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• pp.181-190
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• 2011

본 연구는 백합과 이매패류인 바지락, Ruditapes philippinarum을 노닐페놀에 노출시켰을 때, 생식 및 조직학적 지표 변화에 대해서 조사하였다. 실험기간은 16주였다. 실험구는 1개의 대조구와 3개의 노닐페놀 노출구인 0.05, 0.1, $0.2 mg\;NP\;L^{-1}$였다. 생식소지수는 암컷의 경우, 대조구보다 노출구에서 더 높았으며, 수컷의 경우, 대조구보다 노출구에서 더 낮았다. 이성생식세포 발현율은 14.56%였으며, 수컷에서의 발현율이 더 높았다. 기관계 가운데 외투막에서 혈림프동의 확장, 내부상피층 자유면에 발달하는 선조연의 탈락과 박리, 점액세포의 변성이 관찰되었다. 아가미에서는 정단섬모와 측면섬모의 부분적 소실, interlamella junction 붕괴, 점액세포의 변성이 확인되었다. 발에서는 상피세포의 증식, 선조연의 소실 및 섬모가 탈락되었다. 지방갈색소 축적은 노닐페놀에 농도 의존적으로 높았다. 본 연구결과 노닐페놀은 바지락의 낮은 생존율과 조직병리학적 변화 및 이성생식세포 발현을 유도하며, 더욱이 생식과 관련하여 estrogenic effector로서 가능성을 확인하였다.

• 생명과학회지
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• 제22권8호
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• pp.1009-1017
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• 2012

Rodobacter sphaeroides에서 orf282 유전자는 cbb3 terminal oxidase를 암호화하는 ccoNOQP 오페론과 혐기적 활성자인 FnrL을 암호화하는 fnrL 유전자 사이에 있으며, 아직은 기능이 잘 알려지지 않았다. orf282 유전자의 기능을 알기 위해 우리는 orf282의 일부를 삭제함으로써 유전자를 붕괴시켜 orf282-minus mutant를 제조하였다. 두개의 FnrL 결합 부위가 orf282의 upstream에 존재한다는 것이 밝혀져 있으며, orf282 유전자가 FnrL에 의해 양성적으로 조절된다는 것이 증명되었다. orf282 유전자는 B875와 B800-850 spectral complexes의 형성과 관련이 없다. orf282 mutant에서의 cbb3 oxidase 활성을 wild type와 비교해보면 orf282 유전자가 ccoNOQP 오페론의 조절과 cbb3 cytochrome c oxidase의 생합성과 무관하다는 것을 알 수 있다. orf282 mutant의 구조 유전자인 nifH와 조절유전자인 nifA의 프로모터 활성이 증가한 것은 orf282 유전자 산물이 nifH와 nifA의 발현에서 음성적 effector로 작용한다는 것을 시사한다.