• Title/Summary/Keyword: effective extraction

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Determination of capsaicinoids in red pepper powder using ultra high performance liquid chromatography (초고속 액체크로마토그래피를 이용한 고춧가루의 capsaicinoids 분석)

  • Namgung, Bae;Lee, Yunyeol;Ha, Jaeho
    • Analytical Science and Technology
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    • v.26 no.4
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    • pp.256-261
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    • 2013
  • The efficiency of the two extractions (refluxing extraction and heating-block extraction) was compared to develop the simple analytical method for the determination of capsaicinoids, including capsaicin and dihydrocapsaicin in red pepper powder. For the method development, the parameters, including particle size, extraction time and sample size, were evaluated using ultra high performance liquid chromatography (u-HPLC). It was found that the most effective extraction time of the refluxing extraction was 3 hr. The higher extraction efficiency was obtained with the fine particle of a mild red pepper powder, while the particle size did not affect the extraction efficiency in case of the hot red pepper powder. The higher extraction efficiency was obtained with the small size of sample taken because of the ratio of the large extracting solvent to sample amount. The extraction efficiency of the refluxing method was 3-9% higher than that of the heating-block method, however, the heating-block method could be applied to the determination of capsaicinoids in the red pepper powder for the purpose of quality control of the product.

Solidification and Stabilization of Metal(loid)s-contaminated Soils using Single Binders (단일 고형화제를 이용한 중금속류 오염 토양의 고형화/안정화)

  • Park, Hye Ok;Choi, Jiyeon;Oh, Sanghwa;Shin, Won Sik
    • Journal of Soil and Groundwater Environment
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    • v.20 no.7
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    • pp.135-147
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    • 2015
  • Remediation of metal(loid)s-contaminated sites is crucial to protect human and ecosystem. Solidification and stabilization of metal(loid)s by the binder amendment is one of the cost-effective technologies. In this study, metal (loid)s in various field-contaminated soils obtained from steel-making, metal refinery and mining tillage were immobilized by the application of single binders such as diammonium phosphate (DAP), lime, and ladle slag. The efficiency of solidification and stabilization was evaluated by Toxicity Characteristic Leaching Procedure (TCLP) and the Standard, Measurements and Testing programme of European Union (SM&T) extraction processes. In terms of TCLP extraction, the binder was effective in order of lime > DAP > ladle slag. All binders were highly effective in the immobilization of Pb, Zn, Cu, Ni, and Cd. The increased immobilization efficiency is attributed to the increase in the Step III and IV fractions of the SM&T extraction. Lime and ladle slag were highly effective in the immobilization of the metal(loid)s, however, As release increased with DAP due to competition between the phosphate originated from DAP and arsenate. A further study is needed for the better immobilization of multi metal(loid)s using binary binders.

Extraction of Biomolcules by Ionic Liquids (이온성 액체를 이용한 생물분자의 추출)

  • Lee, Woo Yun;Lee, Yong Hwa;Lee, Jun;Hong, Yeon Ki
    • Journal of Institute of Convergence Technology
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    • v.4 no.2
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    • pp.23-26
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    • 2014
  • As an effective separation method for biomolecules, aqueous two-phase systems based on ionic liquids were suggested. Hydrophobic ionic liquids are more expensive and viscous in spite of their usage in the ionic liquid/water biphasic extraction compared with hydrophilic ionic liquids. In case of aqueous two-phase systems using hydrophilic ionic liquids, they can be diluted in aqueous phase. Experimental results show that aqueous two phase systems can be formed by adding appropriate amount of ionic liquids to aqueous salts solutions. The viscosity of ionic liquid aqueous phase is proportional to the cation chain length in ionic liquids. It is founded that the ionic liquid based aqueous two phase systems are effective for the separation of biomolecules such as acrylic acid.

Accurate RF Extraction Method for Gate Voltage-Dependent Carrier Velocity of Sub-0.1㎛ MOSFETs in the Saturation Region (Sub-0.1㎛ MOSFET의 게이트전압 종속 캐리어 속도를 위한 정확한 RF 추출 방법)

  • Lee, Seonghearn
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.9
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    • pp.55-59
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    • 2013
  • A new method using RF Ids determined from measured S-parameters is proposed to extract the gate-voltage dependent effective carrier velocity of bulk MOSFETs in the saturation region without additional dc Ids measurement data suffering parasitic resistance effect that becomes larger with continuous down-scaling to sub-$0.1{\mu}m$. This method also allows us to extract the carrier velocity in the saturation region without the difficult extraction of bias-dependent parasitic gate-source capacitance and effective channel length. Using the RF technique, the electron velocity overshoot exceeding the bulk saturation velocity is observed in bulk N-MOSFETs with a polysilicon gate length of $0.065{\mu}m$.

A Study on Extracting Ideas from Documents and Webpages in the Field of Idea Mining (아이디어 마이닝 분야에서 문헌과 웹페이지의 아이디어 발췌에 대한 연구)

  • Lee, Tae-Young
    • Journal of the Korean Society for information Management
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    • v.29 no.1
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    • pp.25-43
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    • 2012
  • The ideas and quasi-ideas useful for human's creation were drawn out from documents and webpages with extraction methods used in idea mining, opinion mining, and topic signal mining. The extraction methods comprised (1) decisive cue phrases, (2) cue figures and sounds, (3) contextual signals, and (4) discourse segmentations, They tested on the idea samples, such as thoughts, plans, opinions, writings, figures, sounds, and formulas. Methods (1), (3), and (4) received largely positive evaluation, judging the efficiency of 4 methods by F measure, a mixture of recall and precision ratio. In particular, decisive cue phrase method was effective to search idea and contextual signal method was effective to detect quasi-idea.

The Effects of Hyperbaric Oxygen Therapy on Extraction Wound Healing of Streptozotocin-Induced Diabetic Rats. (당뇨백서 발치창 치유시 고압산소 요법이 미치는 영향에 관한 실험적 연구)

  • Jun, Dong-Ho
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.12 no.1
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    • pp.14-26
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    • 1990
  • This study was undertaken to observe the histopathologic changes of the extraction wounds of diabetic rats when exposed to hyperbaric oxygen. A total of 50 rats (Spraque-Dawley strain) were used, which were all induced with diabetes using streptozotocin before the experiment. The experimental group was exposed to hyperbaric oxygen at 2.5atm. for 2 hours a day during the experimental period. The obtained results were summarized as follows : 1. Severe infiltration of inflammatory cells was observed in the initial stages of both the control and experimental groups. The infiltration showed a decreasing tendency at 3rd week in the experimental group, while severe inflammatory infiltration observed in the control group during the entire experimental period. 2. There was abundant proliferation of capillary blood vessels at 1st week after extraction in the experimental group, while moderate capillary growth in the control group was observed at 1st week after extraction. 3. Osteoblastic activity was started at 1st week after extraction in the experimental group, but there showed markedly delayed appearance in the control group, which showed at 2nd week after extraction. 4. The proliferation of fibroblasts showed rather in the experimental group at 1st week, but it was moderate in the control group at 1st week, 2nd week and 3rd week. 5. Epithelialization of the extraction wound was started at 2 days after extraction and reached its peak at 3rd week in the experimental group, while control group seemed to be delayed and incompleted during the experiment. With regard to these results, hyperbaric oxygen therapy disclosed an effective results on the healing of the extraction wound in diabetic rats under exposure at 2.5 atm for 2 hours daily.

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Risk factors of osteonecrosis of the jaw after tooth extraction in osteoporotic patients on oral bisphosphonates

  • Jeong, Ho-Gul;Hwang, Jae Joon;Lee, Jeong-Hee;Kim, Young Hyun;Na, Ji Yeon;Han, Sang-Sun
    • Imaging Science in Dentistry
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    • v.47 no.1
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    • pp.45-50
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    • 2017
  • Purpose: The aim of this study was to investigate the incidence of bisphosphonate-related osteonecrosis of the jaw (BRONJ) after tooth extraction in patients with osteoporosis on oral bisphosphonates in Korea and to evaluate local factors affecting the development of BRONJ. Materials and Methods: The clinical records of 320 patients who underwent dental extraction while receiving oral bisphosphonates were reviewed. All patients had a healing period of more than 6 months following the extractions. Each patient's clinical record was used to assess the incidence of BRONJ; if BRONJ occurred, a further radiographic investigation was carried out to obtain a more definitive diagnosis. Various local factors including age, gender, extraction site, drug type, duration of administration, and C-terminal telopeptide (CTx) level were retrieved from the patients' clinical records for evaluating their effect on the incidence of BRONJ. Results: Among the 320 osteoporotic patients who underwent tooth extraction, 11 developed BRONJ, reflecting an incidence rate of 3.44%. Out of the local factors that may affect the incidence of BRONJ, gender, drug type, and CTx level showed no statistically significant effects, while statistically significant associations were found for age, extraction site, and duration of administration. The incidence of BRONJ increased with age, was greater in the mandible than the maxilla, and was associated with a duration of administration of more than 3 years. Conclusion: Tooth extraction in patients on oral bisphosphonates requires careful consideration of their age, the extraction site, and the duration of administration, and close postoperative follow-up should be carried out to facilitate effective early management.

Comparison of Preparation Methods for the Quantification of Ginsenosides in Raw Korean Ginseng

  • Hong, Hee-Do;Sim, Eun-Mi;Kim, Kyung-Tack;Rho, Jeong-Hae;Rhee, Young-Kyung;Cho, Chang-Won
    • Food Science and Biotechnology
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    • v.18 no.2
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    • pp.565-569
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    • 2009
  • This study was conducted to evaluate the effects of different preparation methods on the recovery and quantification of ginsenosides in raw Korean ginseng (Panax ginseng C.A. Meyer). Eight major ginsenosides ($Rb_1$, $Rb_2$, $Rb_3$, Rc, Rd, Re, Rf, and $Rg_1$) were analyzed by high performance liquid chromatography (HPLC), after which the recovery and repeatability of the extraction of those ginsenosides using 3 different preparation methods were compared [A. direct extraction (DE) method, hot MeOH extraction/evaporation/direct dissolution; B. solid phase extraction (SPE) method, hot MeOH extraction/evaporation/dissolution/$C_{18}$ cartridge adsorption/MeOH elution; C. liquid-liquid extraction (LLE) method, hot MeOH extraction/evaporation/dissolution/n-BuOH fractionation]. Use of the DE method resulted in a significantly higher recovery of total ginsenosides than other methods and a relatively clear peak resolution. Use of the SPE and LLE methods resulted in clearer peak resolution, but lower ginsenoside recovery than the DE method. The LLE method showed the lowest ginsenoside recovery and repeatability among the 3 methods. Given that the DE method employed only extraction, evaporation, and a dissolution step (avoiding complicate and time consuming purification), this technique may be an effective method for the preparation and quantification of ginsenosides from raw Korean ginseng.

Physicochemical Characteristics and Antioxidant Activities of Omija (Schizandra chinensis Baillon) Seed Oil Extracted at Different Temperatures and Moisture Contents (추출온도 및 함수율에 따른 오미자 종자유의 이화학적 특성 및 항산화활성)

  • Park, Ye Gun;Park, So Yeon;Park, Youn-Je
    • The Korean Journal of Food And Nutrition
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    • v.35 no.1
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    • pp.62-73
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    • 2022
  • Omija seed oil was extracted from Omija seeds, a by-product of Omija, using an eco-friendly pressed extraction method. Physicochemical properties and antioxidant activities of 12 extracts using different combinations of seed moisture content (5, 7.5, and 10%) and extraction temperature (25, 49, 75, and 100℃) were then investigated. The highest extraction yield was 31.33% at moisture of 5% and temperature of 75℃. The lowest acid value was 4.18±0.25 at moisture of 5% and temperature of 49℃or moisture of 7.5% and temperature of 25℃. Peroxide value, iodine value, and saponification value were the lowest at 0.64±0.56 meq/kg, 159.38± 6.03, and 57.60±9.40, respectively, at moisture of 5% and temperature of 25℃. The content of total polyphenolics was the highest at 4,413±125 mg TAE/100 g when the moisture content was 10% and the extraction temperature was 25℃. DPPH radical scavenging activities of oil extracts at 20~100 mg/mL were 28.68±7.30~87.65±2.20%. The maximum antioxidant activity and IC50 were 87.65±2.20% and <20 mg/mL, respectively, for extract obtained at moisture of 10% and temperature at 100℃. As a result, the extraction yield, acid value, peroxide value, iodine value, saponification value, and so on were excellent at moisture content of 5% and extraction temperature of 25℃ or 49℃. However, the content of total polyphenolic compounds and antioxidant activity were the highest at moisture of 10% and extraction temperature of 100℃. In conclusion, extracting Omija seed oil from Omija seeds, a by-product of Omija, is effective with a pressed extraction method.

Optimization of Extraction Condition on Fig (Ficus carica L.) by Response Surface Methodology (반응표면분석법에 의한 무화과 열수 추출조건의 최적화)

  • Kim, Jung-Ok;Kwon, Soon-Tae;Lee, Gee-Dong;Hong, Joo-Heon;Moon, Doo-Hwan;Kim, Tae-Wan;Kim, Dae-Ik
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.66-73
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    • 2008
  • Response surface methodology (RSM) was applied for monitor the yields of desirable substances from fig (Ficus carica L) under different extraction conditions. The maximum yield was 66.46% at 22.08 mL/g of solvent to sample ratio, $90.59^{\circ}C$ extraction temperature and 148.04 min extraction time. The maximum total phenolics was $121.31{\mu}g/mL$ at 17.87 mL/g, $98.82^{\circ}C$, and 130.80 min. The maximum electron donating ability was 54.09% at $121.31{\mu}g/mL$, 18.13 mL/g, and $98.81^{\circ}C$. The maximum value of protease activity was 54.51 unit/min at 17.45 mL/g, $99.01^{\circ}C$, and 131.43 min. In addition, the maximum value of reducing sugar content was 19.14 mg/mL in 22.66 mL/g, $86.30^{\circ}C$, and 153.59 min. The optimum conditions estimated by RSM for maximal extraction of the effective components were $17{\sim}25$ mL/g of solvent to sample ratio, $80{\sim}100^{\circ}C$ of extraction temperature, and $100{\sim}170$ min of extraction time.