• Proceedings of the Korean Aquaculture Society Conference
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• 2004.05a
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• pp.205-206
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• 2004

• Applied Biological Chemistry
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• v.39 no.4
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• pp.282-286
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• 1996

To effectively utilize fish skin gelatin as a material for quality improvement in surimi gel from fish with a red muscle, conger eel skin gelatin was modified with succinic anhydride, and funtional properties such as emulsifying activity and emulsifying stability were determined. The degree of chemical modification incresed up to 0.3 g of succinic anhydride/g of gelatin, above this adding ratio a nearly constant value was reached. The maximum amount of modification was about 90%. The emulsifying activity and emulsifying stability of gelatin gradually increased up to 89.8% of succinylation extent, little changed above of succinylation extent. The other functional properties as solubility, water holding capacity, foam expansion and foam stability were improved following succinylation with 0.3 g of succinic anhydride/g of gelatin. Amino acid composition of succinylated gelatin was similar to that of unmodified gelatin. Heavy metal contents such as cadmium, lead, copper and zinc of succinylated gelatin were lower than those of unmodified gelatin.

• Parasites, Hosts and Diseases
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• v.30 no.3
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• pp.157-162
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• 1992

Although the sea eel (Astroconger myriaster) is suspected as one of the most important fish host for human anisakiasis in Korea, no report has been made on the infection status of the sea eel with anisakid larvae. In the present study, 26 sea eels (Astroconger myriaster) were purchased from the Noryangjin 6sh market in Seoul, and anisakid larvae were collected from their viscera, muscle, head and skin. The collected larvae were classified by their morphological types. A total of 1,351 anisakid larvae were collected from 15 of 26 fish examined. Among them, 1,269 were recovered from the viscera, 66 from the muscle, and 16 from the head and skin. Morphologically, most of the anisakids were classified into 6 known larval types, Anisakis type I (564 larvae) of Berland(1961) , Contracaecum type A(409) and type D(5) of Koyama et at. (1969), Contracaecum type C'(83) and type D'(117) of Chai et at. (1986), and Contracaecum type V(1) of Yamaguti (1935). Remaining 172 specimens were new in the available literature, hence, designated as Centracaecum type A'(new type). The present results revealed that the sea eels caught in the Korean waters are heavily infected with anisakid larvae, not only in their viscera but also in the muscle, and Anisakis type I was the most common among the 7 larval types.

• Journal of Pharmacopuncture
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• v.13 no.1
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• pp.53-61
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• 2010

Purpose : To investigate the effects of the lavender, lemon and eucalyptus oil mixture on the atopy dermatitis skin lesions induced on NC/Nga Mice by dinitrochlorobenzene (DNCB). Material and Method : For this purpose, we fabricated the oil mixture blending three essential oils (lavender, lemon, eucalyptus : ELL) with one carrier oil (jojoba) and apply it on the atopic dermatitis skin lesions of NC/Nga Mice. Atopic dermatitis in NC/Nga Mice was induced by DNCB treatment on the dorsal skin of mice for 8 weeks. The mixture of ratio of each essential oil drop was 1 (eucalyptus) : 2 (lemon) : 2 (lavender) and this mixture was blended with jojoba oil 50ml (0.025%). The ELL-ointment was supplied for 8 weeks. We evaluated the effects of ELL on cell viability of mouse lung fibroblast, clinical skin features and severity, the level of serum Immunoglobulin (Ig) E & Ig G1, Interleukin (IL)-4, IL-13 and Interferon (IFN)-$\gamma$. Results : ELL showed safety on the cell viability of mouse lung fibroblast compared with control group. The cell viability was measured by SRB method. The effects of ELL on clinical skin features and severity in DNCB-induced dermatitis model of NC/Nga mice was significant compared with control group. EEL also showed significant effects on clinical symptom score compared with control group. Serum IgE & IgG1 level and development of atopy dermatitis skin lesions were evaluated. Serum IgE & IgG1 production was significantly down-regulated in EEL group compared with control group. ELL also down-regulated the levels of IL-4 and IL-13, and up-regulated the level of IFN-$\gamma$ compared with control group significantly. Conclusion : ELL was effective on atopy dermatitis by modulating Th2 related factors.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.103-103
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• 1995

There have been several reports regarding the effects of Panax ginseng on allergy reactions. However, they are very sporadic and no systemic yet. To study the effects of Panax ginseng on hypersensitivity, either ginseng total saponin (GTS, 200mg/kg, oral, two hours prior to experiments) or ethanol extract (50 and 200 mg/kg, oral, one week) was administered. Various parameters were employed to assess the anti-allergic actions of Panax ginseng 48hr passive cutaneous anaphylaxis (PCA), skin reactions, histamine release from rat peritoneal mast eel Is, and lipoxygenase activity. In 48hr PCA, and in skin reactions induced by chemical mediators (histamine, serotonin) and mediator releaser (compound 48/80), Panax ginseng did not suppress sensitized immune functions, rather showed tendency to increase the histamine-induced vascular permeabi1ity. Panax ginseng did not inhibit lipoxygenase activity either.

• Journal of fish pathology
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• v.25 no.3
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• pp.151-158
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• 2012

Diseased eel (Anguilla bicolor) displayed severe hemorrhages in the gills, and congestion and swelling in the liver. During the epizootic, the water temperature was $28^{\circ}C$ and the morality rates were about 5%. No parasites were found on the gills and skin. Bacteria were not cultured from any internal organs using TSA or SS agar at $28^{\circ}C$ for 48 hrs. Histopathologically, the gills showed epithelial hyperplasia in the base of secondary gill lamellae and hemorrhages in the capillaries. Some cells in the proliferated interlamellar epithelia exhibited marginal hyperchromatosis. And severe vacuolated changes in the parenchymal cells and congestion in the central veins were observed in the liver. The specific amplicon (396 bp) was detected from gills and opercula of affected eel PCR using Anguillid herpesvirus-1 (AngHV-1) -specific primer sets HVAPOLVPSD (5-'GTG TCG GGC TTT GTG GTG C-3') and HVAPOLOOSN (5'-CAT GCC GGG AGT CTT TTT GAT-3'). Sequencing analysis of the amplicon demonstrated that this gene was 99% homologous to the AngHV-1 sequence deposited in GenBank. This is the first report of AngHV-1 outbreak in the farmed shortfin eels (A. bicolor) in Korea. When diseased fish were maintained for 10 days at water temperatures of $32^{\circ}C$ and $35^{\circ}C$, the cumulative mortalities were 100% and 10%, respectively. Even though the AngHV-1 genome in the gills from the eel kept at $35^{\circ}C$ was detected using PCR, the structure of gill filaments was similar with that of normal fish. Increasing the water temperature to $35^{\circ}C$ was an effective way to diminish the mortality of AngHV-1 affected eel.

• Journal of fish pathology
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• v.13 no.2
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• pp.75-86
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• 2000

Monogeneans(Phylum Platyhelminthes) have been known as common parasites onto the skin and gills of cultured freshwater fishes. Infestation with these parasites result in a great loss in aquaculture industry. Some classification studies on these parasites have mostly been conducted in Japan but rarely in Korea. For the purpose of classifying monogenean parasites infesting two Korean freshwater fishes, eel (Anguilla japonica) and catfish(Parasilurus asotus), samples captured from March 1998 to April 2000 were examined. Here we report for the first time in Korea that four different species of monogeneans are identified: Pseudodactylogyrus bini and P. anguillae in eels; Ancylodiscoides infundibulovagina and Ancylodiscoides sp. in catfish.

• Applied Biological Chemistry
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• v.39 no.5
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• pp.361-367
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• 1996

As a part of investigation for quality improvement of surimi gel from fish with a red muscle by addition of emulsion curd, we investigated the processing conditions of emulsion curd contained succinylated gelatin from conger eel skin as an emulsifier and emulsion curd-added surimi gel. Activity and stability of emulsion curd on standing at room temperature, chilled temperature and vibration were remarkably improved by the addition of 15 tunes of soybean oil and 5 times of water to succinylated gelatin from conger eel skin. The proximate composition of the emulsion curd was moisture 18%, protein 5%, lipid 76% and ash 0.5% and its appearance was white. Peroxide value and fatty acid composition of emulsion curd contained succinylated gelatin as an emulsifier were similar to these of soybean oil. By the addition of 6% of emulsion curd to mackerel surimi, gel strength, appearance and texture of the resulting surimi gel were improved, while its peroxide value and brown pigment revealed minor change. From the results of volatile basic nitrogen, viable cell counts and histamine content, the emulsion curd-added mackerel surimi gel can be safe In the sense of food sanitation.

• KSBB Journal
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• v.26 no.3
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• pp.260-265
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• 2011

This study aimed to examine the treatment effect of Muraenesox cinereus extract product combined with high frequency on men's damaged skin. The subjects were treated by a product containing M. cinereus extract combined with high frequency therapy for six weeks and tested in skin condition. First, for moisture level change, there was no significant difference between the control group treated only by high frequency therapy and the experimental group treated by a M. cinereus extract product combined with high frequency therapy. But the subjects who increased consistently in moisture level in the cheek and chin were more common in the experimental group. Second, for an oil level, prominently high increase was found in both the control group and the experimental group. Finally, for a rough level, while there was little difference in the control group between immediately after peeling and after six weeks, high improvement effect on a curve was found in the experimental group. Based on this result, cosmetics using M. cinereus extract may have a positive effect on men's skin which is exposed to several stress factors. Accordingly, this result will contribute to developing men's functional cosmetics.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.2
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• pp.575-579
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• 2004

4-Geranyloxy compound (1) has elucidated by spectroscopic analysis. This compound (1) inhibited the growth of the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (2 mm inhibition zone at 15 ㎍/disc), cytotoxic to P388 murine leukaemia eel/lines ATCC CCL 46 P388D1, (IC/sub 50/ 1,125 ng/ml at 7.5㎍/disc) and BSC monkey kidney cell lines (100% of well at 15 ㎍/disc).