• Title/Summary/Keyword: edible leaf

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Canna edulis Leaf Extract-Mediated Preparation of Stabilized Silver Nanoparticles: Characterization, Antimicrobial Activity, and Toxicity Studies

  • Otari, S.V.;Pawar, S.H.;Patel, Sanjay K.S.;Singh, Raushan K.;Kim, Sang-Yong;Lee, Jai Hyo;Zhang, Liaoyuan;Lee, Jung-Kul
    • Journal of Microbiology and Biotechnology
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    • v.27 no.4
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    • pp.731-738
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    • 2017
  • A novel approach to synthesize silver nanoparticles (AgNPs) using leaf extract of Canna edulis Ker-Gawl. (CELE) under ambient conditions is reported here. The as-prepared AgNPs were analyzed by UV-visible spectroscopy, transmission emission microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, energy-dispersive analysis of X-ray spectroscopy, zeta potential, and dynamic light scattering. The AgNPs showed excellent antimicrobial activity against various pathogens, including bacteria and various fungi. The biocompatibility of the AgNPs was analyzed in the L929 cell line using NRU and MTT assays. Acridine orange/ethidium bromide staining was used to determine whether the AgNPs had necrotic or apoptotic effects on L929 cells. The concentration of AgNPs required for 50% inhibition of growth of mammalian cells is far more than that required for inhibition of pathogenic microorganisms. Thus, CELE is a candidate for the eco-friendly, clean, cost-effective, and nontoxic synthesis of AgNPs.

Physicochemical Properties and Antioxidant Activity of Extract from Astragalus membranaceus Bunge Leaf Fermented with Lactic Acid Bacteria (유산균으로 발효한 황기 잎 추출물의 이화학적 특성 및 항산화 활성)

  • Song, Bit Na;Lee, Da Bin;Lee, Sung Hyun;Park, Bo Ram;Choi, Ji Ho;Kim, Yong Suk;Park, Shin Young
    • Korean Journal of Medicinal Crop Science
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    • v.28 no.6
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    • pp.428-434
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    • 2020
  • Background: This study aimed to investigate the quality characteristics of Astragalus membranaceus Bunge leaf (AMBL) fermented with lactic acid bacteria and the applicability of its biologically active compounds. Methods and Results: An assessment of physicochemical properties such as pH, total acidity, free sugars, and isoflavonoid (calycosin-7-o-β-d-glucoside, ononin, calycosin, and formononetin) was conducted. Furthermore, the levels of antioxidant compounds, including polyphenols and flavonoids, and radical scavenging activities of the extracts using 2,2-Diphenyl-1-picryl-hydrazyl-hydrate and 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were investigated. The calycosin content in the water extract of AMBL fermented with Leuconostoc mesenteroides increased by approximately twice as much as the control. Conclusions: These results indicate that L. mesenteroides can be used to improve biological activity through fermentation, and that AMBL can be used as a functional materials and edible resource in industrial areas.

Automatic Recognition System for Wild edible greens using Leaf External Form (잎의 외형을 이용한 산나물 자동인식 시스템)

  • Kim, Seong-Jung;Ju, Hwi-Yoon;Kim, Hyun-Jung;Won, Il-Yong
    • Proceedings of the Korea Information Processing Society Conference
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    • 2014.11a
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    • pp.980-983
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    • 2014
  • 본 논문은 잎의 외형을 이용한 산나물 자동 인식 시스템을 제안한다. 더 나은 산나물 자동 인식 시스템을 위해 잎의 외형 추출뿐만 아니라 방향벡터를 이용하여 정확도를 향상시켰다. 이를 위해 BP와 HMM 알고리즘을 이용하여 개선하였고, 외형 특징점을 추출하여 표현하였다. 제안하는 시스템의 성능은 실험으로 증명하였으며, 어느 정도 의미 있는 결과를 얻을 수 있었다.

The Effects of the length of Electrical Stimulation Treatment Time on the Yield Components and $NO_3^-$ Content in Edible Parts in 2 Cherry Type Radishes(Raphanus salivas L.). (電氣刺戟 處理時間이 赤丸20일 무의 收量形質 및 可食部內 $NO_3^-$含量에 미치는 影響)

  • 권오달
    • Korean Journal of Organic Agriculture
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    • v.10 no.1
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    • pp.65-74
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    • 2002
  • The results of the experiment about the effect of the length of electrical stimulation treatment time on the yield components and NO$_3$ content in edible parts of 2 cherry type radishes(Raphanus salivas L.) were like followings. 1. The 2 hours electrical stimulation promoted the length of leaf and the enlargement of the root diameter of 'Comet' and 'Sakuranbo' cultivars, and their root diameters were increased by 14.7% and 12.8% respectively than that of the control. 2, The plot which showed the highest root fresh weight was the 2 hours stimulation plot in 'Comet' and 8 hours stimulation plot in 'Sakuranbo', and these results were approximately 39% higher than that of the control. 3. All of the electrically stimulated plots of 'Comet' and 'Sakuranbo' showed higher NO$^{[-10]}$ $_3$content in roots than the control. And the amount of NO$^{[-10]}$ 3 in their roots showed a remarkable positive correlation with the root diameter, the root fresh weight and the total fresh weight.

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Plant Regeneration via Somatic Embryogenesis and Organogenesis from Peucedanum japonicum $T_HUNB$ (갯기름나물의 체세포배 분화와 기관 분화를 통한 재분화)

  • Kim, Ok-Tae;Kim, Kwang-Soo;An, Jun-Chul;Hwang, Baik
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.1
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    • pp.21-24
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    • 2001
  • Peucedanum japonicum $T_{HUNB}$ used as a edible and medicinal plants was investigated for in uitro regeneration. Callus formation occurred on leaf and stem explant cultures and showed spontaneous embryogenic and organogenic capability on MS basal medium supplemented with 0.1~5 mg/L NAA and 0~10 mg/L BA in dark. The regeneration was highest on the condition supplemented with 2.5 mg/L NAA and 10 mg/L BA. Development of the somatic embryo progressed through the globular, heart-shaped, torpedo-shaped and cotyledonary stage, typical of zygotic embryos. When the first somatic embryos was cultured on the medium supplemented with 0.2 mg/L NAA, secondary somatic embryo were induced with higher frequency on the hypocotyl then on the cotyledon and root.t.

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The Usage of Regional Folk Plants in Gyeongsangnam-do (경상남도 지역의 민속식물 이용현황)

  • Im, Hyoung-Tak;Hong, Hang-Hwa;Son, Hyun-Duk;Park, Myung-Soon;Nam, Bo-Mi;Kwon, Bo-Kyeong;Lee, Cheul-Ho;Chung, Gyu-Young
    • Korean Journal of Plant Resources
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    • v.24 no.4
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    • pp.419-429
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    • 2011
  • This study was carried out to classify the regional folk plants in Gyeongsangnam-do. According to the survey results from 293 survey sheets of 60 residents at 52 places of 17 counties and cities from March to October, 2009, the folk plants in Gyeongsangnam-do consisted of a total 182 taxa; 73 families, 157 genera, 168 species, 2 subspecies, 11 varieties, and 1 form. The use by its usage was: 129 taxa; edible, 41 taxa; medicinal, 3 taxa; dye, 2 taxa; aroma, 2 taxa; spice, 2 taxa; ornamental, 6 taxa; fiber, 2 taxa; oil, 25 taxa; others, respectively, so the edible use was the highest. The most useful part was the leaf, followed by all parts, fruit and root.

Synthetic Seed Development and Production for Industrialization of Eastern Bracken (고사리 산업화를 위한 인공종자 개발 및 생산)

  • Bo Kook Jang;Ju Sung Cho;Cheol Hee Lee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.11-11
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    • 2021
  • Ferns have been consumed as food in many countries for centuries. As rich sources of protein, fiber, minerals, vitamins, essential amino acids, and fatty acids, ferns provide important nutrients to humans. Eastern bracken (Pteridium aquilinum var. latiusculum (Desv.) Underw. ex A. Heller) is the most popular edible fern in South Korea where, additionally, it has long been used as an edible wild leaf vegetable. Recently, the production of eastern brackens in South Korea (2018) has reached 14,032 tons, for an annual revenue of 83.5 billion won, and even more eastern brackens are marketed if imports are taken into account as well. Most of the common ferns can be propagated using spores. However, fern farmers cultivate seedlings through traditional propagation methods, such as root pruning or rhizome division. These propagation methods exhibit limitations in forming roots and growing-points and are labor intensive. Quality seedlings of eastern bracken can be obtained through spore propagation, but the spores are fine and difficult to handle in the field. In addition, it would require appropriate environmental control. The production of synthetic seeds using encapsulation technology is easy to establish and it can be used to achieve high productivity at low cost. Synthetic seeds contain explants embedded into a seed foam, and they overcome the limitations of micropropagation and offer the possibility of using plug seedlings. Synthetic seed matrix, such as sodium alginate, has the advantages of low cost, low toxicity, and gel stability. The present study aimed to develop and produce synthetic seeds for the commercial exploitation of eastern bracken. Furthermore, we verified spore germination and the extent of gametophyte and sporophyte development achieved with our new synthetic seeds, whose production was intended to solve current problems with the handling, storage, and transportation of eastern bracken.

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The Growth and Physiological Responses of Cacalia firma Seedlings by Shading Conditions in Forest Farming (임간재배 시 병풍쌈 유묘의 차광처리별 생장 및 생리 반응)

  • Yoon, Jun Hyuck;Jeon, Kwon Seok;Song, Ki Seon;Park, Yong Bae;Moon, Yong Sun;Lee, Do Hyung
    • Journal of Korean Society of Forest Science
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    • v.103 no.1
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    • pp.65-71
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    • 2014
  • Cacalia firma is a perennial plant in Asteraceae, Parasenecio that distributed in Korea, China, and Japan. As dietary style changes for well-being life, consumer's demand of functional food and organic vegetables is getting increased. This study was conducted to investigate the optimum light conditions of P. firmus in forest farming. One year old seedlings were grown under four different light conditions 10%, 20%, 30%, and 50% of sunlight by shading (equals 50%, 30%, 20%, and 10% relative brightness respectively) and non-treated control under full sunlight. They were analyzed for early growth and physiological response. Seedlings grown under 75% shading showed similar height, root growth, and leaf water content to control. However, their leaf length, width, and total leaf area were increased, which caused increased leaf dry weight and total dry weight. Especially, seedlings under 95% shading showed 40% increase in height and more leaf growth and leaf water content, although they had shorter main root length and root collar diameter than control. In addition specific leaf area (SLA) and leaf area ratio (LAR) were higher than control and indicated that they were statistically significant difference from control. Higher SLA refers thinner leaf thickness, higher LAR means larger leaf area. The results indicate seedlings under 95% shading have higher water content, thinner leaf, and wider lightinterception areas. It is plausible that P. firmus is active in chlorophyll activities and carbon dioxide assimilation at even lower light conditions. These results suggest that the optimum light level of P. firmus for artificial cultivation in forest farming ranges from 75~95% shading (20%-10% of relative brightness). When salability as 'sanchae' (wild edible greens) is considered, P. firmus could be cultivated under 75% shading in forest farming and expected to have better taste and higher yield. We suggest these results as basic data of P. firmus for possible forest farming.

Apoptotic Effects of Some Plants on MCF-7 Mammary Gland Adenocarcinoma Cells (수종식물의 MCF-7 세포에 대한 세포사 및 항ㆍ증식효과)

  • 정용자
    • Journal of Life Science
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    • v.14 no.1
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    • pp.61-66
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    • 2004
  • Many studies have been widely carried out to find out new compound having anti-cancer activity from animals and plants. Some plants have been reported to have anti-lancer effects. However, the anti-cancer effect of edible plants were seldomly evaluated. Therefore we investigated the anticancer effects of edible plants (10 samples) easily available around us by measuring number of survival cancer cells after treatment with direct cell counting and MTT analysis, and by examining the morphological change under the electromicroscope. Of the 10 samples tested, Equisetum arvense L., Lactuca dentata Mokino. var. faviflora Makino. showed moderate anti-cancer effects even at the concentration of 10 $\mul/ml$ against MCF-7 adenocarcinoma cell line. Of them, Capsicum annuum L. had most potent anti-cancer activity against MCF-7 adenocarcinoma cell line showing proliferation inhibited, morphological change and apoptosis at the concentration of 2 $\mul/ml$.

Effect of Root Zone Restriction on the Growth Response and Phytonutrients Contents of Leafy Vegetables Grown in a DFT System (담액 수경재배시 근권제한이 엽채류의 생육 및 식물 영양소 함량에 미치는 영향)

  • Seo, Tae-Cheol;Rhee, Han-Cheol;Yun, Hyung-Kwon;Chun, Chang-Hoo
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.415-422
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    • 2010
  • Two leaf lettuces, chicory, endive, edible chrysanthemum, and pak-choi were hydroponically cultured under root restricted conditions in a deep flow technique system and their growth and nutritional values were investigated. Cylindrical plastic tubes with 100 mm height and diameters of 20, 25 or 30 mm were used for root restriction. Growth of all species was retarded by 25-95% as the roots zone was restricted. Pak-choi and edible chrysanthemum showed greater reduction in growth as compared with chicory and endive. Percent dry matter, C/N ratio, and contents of total ascorbic acid and total anthocyanin increased in the root restricted treatments. Contents of P and K decreased in the root restricted treatments, while contents of Mg, Fe, Mn, and Zn were not consistent among the tested species. Optimized root volumes to improve the nutritional values and to reduce the growth retardation varied depending on species of leafy vegetables. Tubes of ${\Phi}25mm{\times}100mm$ and ${\Phi}30mm{\times}100mm$ were suitable for chicory, endive and lettuce, and edible chrysanthemum and pak-choi, respectively. Results indicate that nutritional values of hydroponically-cultured leafy vegetables can be improved by root restriction.