• Journal of Plant Biotechnology
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• 제30권2호
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• pp.161-165
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• 2003

The effect of NAA and Benzyladenine(BA) for determination times on the formation of adventitious shoots, roots trichmoes and calli in MS basal medium was investigated in leaf segments from ecotype 'Nosses' of Arabidopsis thalliana. Adventitious shoots, roots, trichomes and calli were formed fromed from leaf segments in a wede range of NAA and BA. The optimal combination of hormones for adventitious shoots formation, 20mg/L NAA for trichome formation, 100mg/L for callus formation. Inductive times for formation of adventitious shoots, roots, trichomes and calli were determined at 14, 4, 6 and 18 days respectively by periodical transfer of leaf segments from hormines containing media to hormone free medium.

• 식물분류학회지
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• 제51권2호
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• pp.176-180
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• 2021

Arabidopsis thaliana (L.) Heynh. is a small plant species that serves as a model organism of plant biology and genetics. Here, we present the first complete mitochondrial genome of Korean A. thaliana natural isolate (named as 180404IB4), which is 368,875 bp long and contains 58 genes (33 protein-coding genes, 22 tRNAs, and three rRNAs), with a GC ratio of 44.8%. Sixty-four single-nucleotide polymorphisms and 11 insertion and deletion regions (1,089 bp in length) are identified against the Col-0 ecotype, showing one large insertion of 1,069 bp without structural variation. Phylogenetic trees constructed from 30 conserved genes indicate that the 180404IB4 mitochondrial genome is clustered with Col-0 and three East Asian ecotypes.

• 한국작물학회지
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• 제32권1호
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• pp.1-4
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• 1987

인도네시아 발리에서 한국 4개품종을 묘대일수와 재배법을 달러하여 공식한 결과 20일묘와 30일묘간에는 이앙후 출수기까지의 일수, 생육과 수양형질 및 수량에서 차이가 없었으며 시비량이 많고 재식밀도가 낮은 한국재배법이 수량 및 수량 구성요소가 유의성 있는 좋은 성적을 보였다.

• 한국작물학회지
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• 제59권3호
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• pp.230-238
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• 2014

벼 유전자원의 효율적인 보존관리를 위해 종자수명 예측방법을 규명하고자 본 시험을 수행하였다. 효과적인 종자수명 예측 방법을 규명하기 위해 전년도에 수확한 벼 106품종을 대상으로 인위노화처리 방법인 노화촉진(AA)처리, 퇴화조절(CD) 및 건열처리(DHT)를 실시하고, $4^{\circ}C$ 저장고에 26.5년간 보존된 벼 유전자원 3,066점의 종자수명 자료와 비교분석한 결과는 다음과 같다. 벼 유전자원의 효과적인 종자수명 예측 방법으로는 건열처리($90^{\circ}C$, 36시간)였다. 전년도 수확한 벼 품종의 건열처리 후 발아율 성적을 사분위수로 4개의 분류군으로 나누었을 때, 분류군별로 분포하는 벼 생태형별 품종 비율이 $4^{\circ}C$ 저장고 보존자원의 최종발아율에 따른 4개 분류군의 분포비와 흡사하였다. 갱신 된 벼 유전자원을 $4^{\circ}C$ 저장고 보존시 효율적인 첫 활력모니터링 시점은 4개 분류군 중 I군에 속하는 자원은 저장 후 14년, II군, III군, IV군에 속한 자원들은 각각 저장 후 17, 20, 45년을 기준으로 하여 설정할 수 있겠다. 건열처리는 벼 유전자원 종자수명 예측뿐만 아니라 종자은행에서 보존자원의 효율적인 활력검정 주기 설정 및 갱신 주기 결정에도 도움이 되겠다.

• Weed & Turfgrass Science
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• 제5권2호
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• pp.95-100
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• 2016

국내에 종자형 품종 개발은 아직 전무한 상태이다. 한국잔디의 종자형 품종 개발은 국내외 잔디산업 발전을 위해서도 매우 중요한 일이다. 한국잔디 자생지인 국내 동,서, 남해안과 내륙지방에서 277 생태형을 수집 하였고, 이중에서 종자를 맺는 102개 수집계통에 대한 종자 수확량과 발아율을 조사하고자 본 연구를 수행하였다. 종자 수확량은 $0.1{\sim}32.2g\;m^{-2}$의 범위를 보였다. 수확량이 가장 많은 계통은 중엽형인 Z6011로 $32.2g\;m^{-2}$로 나타났다. 수집계통의 발아율은 대부분이 50%이하로 낮게 조사되었다. 발아율이 가장 높은 계통은 78%를 보인 Z2095로 조사되었다. 종자수확량이 높으면서, 발아율이 높은 계통으로는 Z6011, Z6015, Z1075, ZN1008, Z1084 등을 선발하였으며, 대부분 중엽형계통과 Z. japonica 계통이었다. 상기 선발 계통들은 향후 다수확 종자형 품종육성을 위한 유용한 유전자원으로 활용될 수 있을 것으로 생각된다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1994년도 94 Symposium on Plant science September 10, 1994 Ewha Womans University, Seoul, Korea 94 식물학 심포지움 환경 스트레스와 식물의 반응
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• pp.29-39
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• 1994

Serious issues about the changes in the environmental conditions on earth associated with human activities have arisen, and the interest in these problems has increased. It is urgent to determine how the expansion of terrestrial UV-B radiation due to the stratospheric ozone depletion influences living matters. In this connection, we have been investigating the effects of UV-B radiation on the growth of rice cultivars (Oryza sativa L.). We report here some physiological and genetic aspects of resistance to inhibitory effects of UV-B radiation on growth of rice cultivars as described below. Elevated UV radiation containing large amount of UV-B and a small amount of UV-C inhibited the development of plant height, the photosynthetic rate and the chlorophyll content in rice plants in a phytotron. Similar results were obtained in experiments, in which elevated UV-V radiation. Similar results were obtained in experiments, in which elevated UV-B radiation (transmission down to 290 nm) was applied instead of UV-B radiation containing a small amount of UV-C. The inhibitory effects of UV radiation was alleviated by the elevated CO2 atmospheric environment or by the exposure to the high irradiance visible radiation. The latter suggested the possibility that the resistance to the effects of UV radiation was either due to a lower sensitivity to UV radiation or to a greater ability to recover from the injury caused by UV radiation through the exposure to visible radiation. The examination of cultivar differences in the resistance to UV radiation-caused injuries among 198 rice cultivars belonging to 5 Asian rice ecotypes (aus, aman, boro, bulu and tjeleh) from the Bengal region and Indonesia and to Japanese lowland and upland rice groups showed the following: Various cultivars having different sensitivities to the effects of UV radiation were involved in the same ecotype and the same group, and that the Japanese lowland rice group and the boro ecotype were more resistant. Among Japanese lowland rice cultivars, Sasanishiki (one of the leading varieties in Japan) exhibited more resistance to UV rakiation, while Norin 1 showed less resistance, although these two cultivars are closely related. It was thus indicated that the resistance to the inhibitory effects of UV radiation of rice cultivars is not simply due to the difference in the geographical situation where rice cultuvars are cultivated. Form the genetic analysis of resistance to the inhibitory effects of UV radiation on growth of rice using F2 plants generated by reciprocally crossing Sasanishiki and Norin 1 and F3 lines generated by self-fertilizing F2 plants, it was evident that the resistance to the inhibitory of elebated UV radiation in these rice plants was controlled by recessive polygenes.

• Applied Microscopy
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• 제47권3호
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• pp.157-159
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• 2017

This study concerns (i) Botanical characterization of local almond ecotype named "Beldi" based on flowering period and flowers color measurements (ii) Chemical characterization of almond oil such as acidity and peroxide index and specific UV absorbance values (K232 & K270). The color results of 100 flowers analyzed by chromameter shows two different color categories. The first is very light pink to whitish ($L^*=81.58$, $a^*=7.43$, $b^*=8.06$). The second is a dark pink ($L^*=71.64$, $a^*=18.34$, $b^*=4.97$) with a difference in color between the two categories ${\Delta}E=15.07$. Compared to Ferragnes/Ferraduel varieties, Beldi almond kernel has almost a comparable oil yield but different acidity and peroxide values which are 0.81%, $8.12meq/O_2/kg$ and 0.40%, $16.39meq/O_2/kg$ respectively.

• 생명과학회지
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• 제12권6호
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• pp.715-720
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• 2002

Arabidopsis thaliana columbia의 종자에 EMS를 처리하여 돌연변이체들을 만들었고 이중 UV-B에 감수성이 높은 돌연변이체를 골랐다. 이 UV-B 감수성 돌연변이체의 원인 유전자를 밝히기 위하여 교배 실험을 한 결과 이는 Mendel 유전법칙을 따르고 단일 유전자의 돌연변이에 의하여 나타나며 열성 유전을 하는 것으로 밝혀져 이 유전자를 uvs라 하였다. 염색체상의 uvs의 위치를 밝히기 위하여 CAPS maker를 이용한 연관분석을 하고자 하였고 이를 위하여 각각 maker의 primer 10종류를 제작하였다. 이를 이용, 각 PCR 산물에 대하여 uvs mutant와는 다른 제한효소 pattern를 갖는 Lansberg와 uvs mutant를 교배시켜서 얻은 것들로부터 DNA를 추출하여 PCR을 수행하였다. 이들과 자외선과의 감수성을 연관시켜 교차율을 계산한 결과 5번 염색체의 LFY3과 가장 가까웁게 연관되어 있었다.

• 한국잡초학회지
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• 제7권3호
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• pp.247-256
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• 1987

The ability of three Echinochloa colona (L.) Link ecotypes to compete with rice (Oryza sativa L.) was evaluated. For all the ecotypes, E. colona was shorter and produced less leaf area and dry matter than rice at 15 days after seeding (DAS), whereas the reverse was observed from 30 to 60 DAS. Neither E. colona nor rice tillered during the first 15 DAS, but E. colona had greater accumulative tiller length than rice from 15 DAS. Rice absorbed more nitrogen than E. colona at 15 DAS. E. colona absorbed greater amounts of nitrogen, phosphorus, and potassium than did rice between 30 and 60 DAS and increase in its density decreased the nutrients uptake of rice. Differences in the competitive ability of the ecotypes were related to the growth characteristics of the ecotypes and the period of competition between the two species. At the early growth stages the Pangasinan ecotype, which increased plant size rapidly and had a shorter life cycle, was more competitive against rice than the Leyte and South Cotabato ecotypes, which had a longer vegetative growth period. However, the reverse was observed at the later growth stages of the ecotypes.

• ALGAE
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• 제30권3호
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• pp.223-232
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• 2015

More than 20 and 10 clades / ecotypes of Synechococcus and Prochlorococcus, respectively, have been identified in various oceanic regions. However, their diversity has yet to be thoroughly studied in the northwest Pacific Ocean. Further, spatial distribution of Synechococcus clades in the oligotrophic oceans has been scarcely characterized. To elucidate picocyanobacterial lineage distribution in the northwest Pacific Ocean, 16S-23S internal transcribed spacer sequences of picocyanobacteria were sequenced by barcoded amplicon pyrosequencing method. Additional pyrosequencing library using a primer specific for the Synechococcus subcluster-5.1 was constructed to thoroughly understand Synechococcus diversity in the oligotrophic oceans. In warm pool area, Prochlorococcus was predominant and showed a distinct depthpartitioning between HLII and LL ecotypes. Despite low abundances, diverse Synechococcus clades appeared in the oligotrophic open ocean, showing both vertical and horizontal niche partitioning. Clade II was the predominant Synechococcus clade, especially in upper euphotic depths. In shallow and middle euphotic depths, clades UC-A, III, and CRD1 were distributed broadly. However, a distinct shift in the horizontal distribution was found at ca. $20^{\circ}N$. Conversely, clades XVII and CRD2 dominated at deep euphotic depths and constituted a higher proportion than clade II. These niche-partitioning of Synechococcus clades seemed to be related with temperature, nutrient concentration as well as iron concentration.