• 제목/요약/키워드: early production

검색결과 2,381건 처리시간 0.025초

Effect of IPTG Induction on Production of ${\beta}$-Galactosidase-PreS2 Fusion Protein in Recombinant Escherichia coli

  • Nam, Soo-Man;Park, Young-Hoon
    • Journal of Microbiology and Biotechnology
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    • 제1권4호
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    • pp.274-280
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    • 1991
  • Effects of IPTG induction on cell growth and production of ${\beta}$-galactosidase-preS2 fusion protein (${\beta}$gal-preS2) were studied in a defined medium using a recombinant Escherichia coli JM109/pCMHB30. IPTG was added (0.2 mM) to induce the cloned-gene expression in the early-, mid-, and late-log growth phases. The most serious decreases in growth rate and plasmid stability were observed for the induction in the early-log growth phase. The expression level of ${\beta}$gal-preS2 attained by the induction in the mid-log phase was about 0.51 mg fusion protein/mg total cellular protein, which was 2- and 5-fold improvement over the levels obtained with the inductions in the early- and late-log phases. Formation of acidic byproducts including acetate and pyruvate showed different profiles during the fermentation period for each cases of induction; pyruvate was the major byproduct for the induction in the early-log phase while acetate production became more significant for the cases of inductions in the mid- and late-log phases.

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Prediction of 305 Days Milk Production from Early Records in Dairy Cattle Using an Empirical Bayes Method

  • Pereira, J.A.C.;Suzuki, M.;Hagiya, K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권11호
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    • pp.1511-1515
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    • 2001
  • A prediction of 305 d milk production from early records using an empirical Bayes method (EBM) was performed. The EBM was compared with the best predicted estimation (BPE), test interval method (TIM), and the linearized Wood's model (LWM). Daily milk yields were obtained from 606 first lactation Japanese Holstein cows in three herds. From each file of 305 daily records, 10 random test day records with an interval of approximately one month were taken. The accuracies of these methods were compared using the absolute difference (AD) and the standard deviation (SD) of the differences between the actual and the estimated 305 d milk production. The results showed that in the early stage of the lactation, EBM was superior in obtaining the prediction with high accuracy. When all the herds were analyzed jointly, the AD during the first 5 test day records were on average 373, 590, 917 and 1,042 kg for EBM, BPE, TIM, and LWM, respectively. Corresponding SD for EBM, BPE, TIM, and LWM were on average 488, 733, 747 and 1,605 kg. When the herds were analyzed separately, the EBM predictions retained high accuracy. When more information on the actual lactation was added to the prediction, TIM and LWM gradually achieved better accuracies. Finally, in the last period of the lactation, the accuracy of both of the methods exceeded EBM and BPM. The AD for the last 2 samples analyzing all the herds jointly were on average 141, 142, 164, and 214 kg for LWM, TIM, EBM, and BPE, respectively. In the current practices of collecting monthly records, early prediction of future milk production may be more accurate using EBM. Alternatively, if enough information of the actual lactation is accumulated, TIM may obtain better accuracy in the latter stage of lactation.

Domestic Production Process of Early Korean Broadcast CG Equipment

  • Nah, So-Mi
    • Journal of Multimedia Information System
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    • 제8권4호
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    • pp.285-294
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    • 2021
  • The development process of domestic CG (Computer Graphics) needs to be studied by classifying the technical part and the design part. This paper analyzes how early domestic broadcast equipment evolved and focused on what went through the development process. Domestic broadcasting companies have introduced and used overseas equipment and have begun to develop their own technology. The development process of the early domestic production technology of broadcasting stations was classified into Character Generator, On-line Real-Time Graphic and Sport Coder. It was found that the orientation of broadcasting technology was conclusively focused on visualization, with socio-cultural factors acting along with the evolution of hardware and software. This research is meaningful in reorganizing the history of the development process of domestic CG technology in the early days through previous research (primary verification), newspaper articles and news coverage (secondary verification). This paper looks at what is missing by regaining the past of CG, and argues that with the advent of new technologies today, we must develop through appropriate division of roles and collaboration between engineers and designers.

파종기에 따른 호밀 품종간의 사초 생산능력 (Forage Performance Evaluation of Rye Cultivars with Different Sowing Dates)

  • 김수곤;김종덕;권찬호;하종규;김동암
    • 한국초지조사료학회지
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    • 제26권1호
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    • pp.31-38
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    • 2006
  • 본 연구는 파종기에 따른 호밀 품종간의 사초생산능력을 구명하기 위하여 수행하였다. 시험배치는 동일한 포장에서 10처리 3반복의 분할구배치로 주구에는 9월 15일과 9월 30일의 파종시기를, 세구에는 'Kodiak', 'Koolgrazer', 'Danko', 'Homil22', 'Olhomi1' 품종을 두었다. 조기파종시의 건물(DM) 함량은 각각 19.7%로 만기파종시의 17.8% 보다 높았으나, 조단백질(CP) 및 가소화영양소총량 (TDN) 함량에 있어서는 만기파종시 보다 낮았다. 조생품종인 Koolgrazer 및 Olhomi1 품종의 건물함량은 다른 품종보다 건물수량은 높았으나, CP 및 TDN 함량은 다른 품종보다 낮았다. ADF, NDF 및 cellulose 함량은 조기파종이 만기파종시 보다 높았으며, ADL 및 hemicellulose 함량은 조기파종이 만기파종시 보다 낮았다. ADF 및 cellulose 함량은 조생품종인 다른 품종보다 높았으나 ADL 및 hemicellulose 함량은 만생종이 Danko가 다른 품종보다 높았다. 조기파종시의 DM, CP 및 TDN 수량은 각각 10,742, 1,774 및 6,571 kg/ha로 만기파종시의 7,770, 1,288 및 4,882 kg/ha 보다 높았으며 공시품종 간에는 조생품종이 다른 품종에 비하여 높았다. 본 시험에서 얻어진 결론은 사초용 호밀의 파종시기를 고려할 때 수량과 사료가치를 높일 수 있는 방법은 조생호밀품종에 조기파종을 조합하는 사초생산 기술이다.

남부지역 시설하우스 벼 극조기재배의 안전작기 설정 (Optimum Transplanting Time for Extremely Early Rice Greenhouse Cultivation in the Southern Area)

  • 최장수;안덕종;원종건;이승필;윤재탁;김길웅
    • 한국농림기상학회지
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    • 제5권3호
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    • pp.191-199
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    • 2003
  • Optimum transplanting time for extremely early rice cultivation as an after-crop of fruit and vegetables under greenhouse conditions in the southern area was determined. Rice was transplanted on March 10, March 20, March 30, April 10 and April 20 far three years from 1998 to 2000. Meteorological computations for rice production were high for heading between early May and early July, but they were too low for heading between late July and early August. Especially the expected yield predicted with 35,000 spikelets, the average spikelets per $m^2$ for extremely early transplanting. Computation for heading between late July and early August was low by 106 kg/10a compared with that yield at heading during the same period in the field. As the transplanting date in extremely early rice cultivation was earlier) rice growth at early stages was more retarded by low temperature. Rice growth at heading stage recovered with high temperature, showing less difference for the transplanting date. Abnormal tillers occurred by 15.5∼22.2%. The contribution of 1,000 grain weight${\times}$ripened grain ratio to yield of the extremely early rice cultivation in the greenhouse was 50.6%, indicating 16% hi일or than the degree of panicle per $m^2$ on yield. The estimated optimum transplanting time on the basis of yield for the extremely early greenhouse rice cultivation ranged from March 19 to April 28, and the estimated critical transplanting date on the basis of accumulated effective temperature was March 12. Rice reduced the amount of NO$_3$-N by 97.1% and EC by 90.5% in greenhouse soil with continuous fruit/vegetables fer more than a 10-year period, and completely removed the root-knot nematodes.

인공점등에 의한 조기 산란유도 꿩집단의 산란능력과 난형 (Egg Production and Egg Shape of Early Egg Laying Group Induced by Artificial Light in Korean Ring-Necked Pheasant)

  • 양영훈;김대철
    • 한국가금학회지
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    • 제22권1호
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    • pp.7-13
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    • 1995
  • To investigate the response of egg production to the artificial light to induce early egg laying. and the changes of egg weight and egg shape during the egg laying cycle, 30 Korean ring-necked pullets and their 1,284 eggs layed under artificial light control were used in this study. The first egg was ohserved at the age of 31 wk, after 4 wk of stimulating light(16 hour light: 8 hour dark). Egg production rate during seven 2-wk periods from the begining of the first egg was 43.7% and a clear peak egg production(61.7%) was shown at the 4th 2-wk period. The effects of egg production period and pullet on the egg weight, egg length, egg width and egg shape index(width/length) were significant(P<0.01). In the egg production cycle. egg weight, egg length and egg width increased steadly with time. but the value of egg shape index increased up to the 3rd 2-wk period and then decreased. Repeatabilities from intraclass correlation for the egg weight, egg length. egg width and egg shape index were 0.61. 0.53, 0.49 and 0.48, respectively.

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이탈리안 라이그라스 채종시기별 건물생산능력 및 생육특성 (Groeth Charateristics & Matter Yield Ability According to Seed Production Time of Italian Ryegrass)

  • 채재석;김영두;김호중;박태일
    • 한국초지조사료학회지
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    • 제16권1호
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    • pp.39-46
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    • 1996
  • The experiment was conducted to inxvestigate matter production ability and growth characteristic variation for Italian ryegrass seed of introduced variety and the different seed production time of the next generation in paddy field at Iksan and Kyehwa experiment field from 1987 to 1988. Germination rate is good at seed production of 35 days before and after heading with introducced variety. Also, Early growth before wintering, dead leaf rate and regenerating day is same tendendy. Panicle numbers per hill like that and heading date is for 15~20 days after heading seed is late about three days, but 35 days before and after heading seed is early about one day. Fresh and drymatter yield at the different cutting time shows decrease in 15~25 days after heading seed but 35 days before and after heading seed is same or increase trend, it's notable at Kyehwa paddy field in reclaimed land. In case of feed value according to cutting time differ little against introduced seed.

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수확기에 따른 호밀 품종간의 사초 생산능력 (Forage Yield and Quality in Rye Cultivars with Different Harvesting Dates)

  • 김수곤;김종덕;권찬호;하종규;김동암
    • 한국초지조사료학회지
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    • 제26권2호
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    • pp.97-104
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    • 2006
  • 본 연구는 수확기에 따른 호밀 품종간의 사초생산능력을 구명하기 위하여 수행하였다. 시험구는 분할구 배치법으로 주구는 4월 20일과 4월 30일의 수확시기를, 세구는 'Kodiak', 'Koolgrazer', 'Danko', 'Homil22', 'Olhomil' 품종으로 하였다. 조기수확시의 건물 함량은 각각 17.8%로 만기수확시의 20.0% 보다 낮았으나, 조단백질 및 TDN 함량에 있어서는 만기수확시 보다 높았다. 공시품종 간에는 건물, 조단백질 및 TDN 함량에 있어서 유의적인 차이가 있었다. ADF, NDF, ADL 및 cellulose 함량은 조기수확이 만기수확시 보다 낮았으나, hemi-cellulose 함량은 조기수확이 만기수확시 보다 높았다. 공시품종 간에는 조생품종의 섬유소함량이 다른 품종에 비하여 높았다. 조기수확시의 건물, 조단백질, IVDDM 및 TDN 수량은 각각 7,839, 1,468, 6,077 및 5,128kg/ha로 만기 수확시의 11,003, 1,813, 8.032 및 6,857kg/ha 보다 낮았으며 공시품종 간에는 조생품종이 다른 품종에 비하여 높았다. 시험에서 얻어진 결론은 사초용 호밀의 수확시기를 고려할 때 수량과 사료가치를 높일 수 있는 방법은 조생호밀품종에 만기수확을 조합하는 사초생산기술에 의해서 가능하다는 것이다.

콩과식물 차풀, 비수리, 싸리 종자의 채종시기별 성숙도 및 발아특성 (Germination Characteristics and Maturity by Production Time of Chamaecrista nomame, Lespedeza cuneata and Lespedeza bicolor Seed in Fabaceae Plant)

  • 강희경;이자연;송홍선
    • 한국자원식물학회지
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    • 제27권4호
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    • pp.359-364
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    • 2014
  • 충남지역에 자생하는 콩과식물 몇 종의 적정한 채종시기 판단과 발아특성을 평가한 결과는 다음과 같았다. 콩과식물 몇 종류의 종자 성숙도, 착생 종자량, 발아율, 발아일수, 탈립을 고려한 채종적기는 차풀이 10월 초중순, 비수리가 11월 초순, 싸리가 11월 초중순으로 판단되었다. 착생 종자량이 가장 많은 시기는 차풀이 10월 초순, 비수리와 싸리가 각각 11월 초순이었고, 성숙 말기 종자비율이 가장 높은 시기는 차풀이 11월 초순, 비수리와 싸리가 각각 11월 중순이었다. 채종시기별 발아율이 가장 높은 시기는 차풀(99.8%)이 10월 초순, 비수리(68.3%)가 11월 중순, 싸리(24.3%)가 11월 초순이었고, 채종시기별 발아일수가 가장 짧은 시기는 차풀이 10월 중순, 비수리와 싸리가 각각 11월 중순이었다. 공시종자의 발아적온은 차풀이 $15{\sim}35^{\circ}C$, 비수리가 $25{\sim}30^{\circ}C$이었고, 싸리는 발아율이 낮아 발아적온의 판단이 어려웠다.

풋콩 재배지에 따른 파종시기가 종자생육에 미치는 영향 (Effect of Planting Time on Seed Production of Vegetable Soybean at Different Locations)

  • 백인열;신두철;박창기;이진모;서형수
    • 한국작물학회지
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    • 제40권1호
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    • pp.44-51
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    • 1995
  • 도입에 의존하고 있는 풋콩 종자 생산체계를 확립하고자 조숙성 품종인 큰올콩과 풋콩 도입풍종인 Fukura 피두를 평야지(영남농업시험장) 및 고랭지(경남 밀양군, 사자평 :해발 850m)에 파종시기(5월 15일, 6월 15일, 7월 15일)를 달리하여 생육 및 수량과 병해립 및 저장후 발아력을 조사한 결과를 요약하면 다음과 같다. 1. 파종시기가 빠를수록 협수가 많고 100립종은 무거웠으며 수량도 높았다. 재배지별로는 평야지가 고랭지보다 증수되었고, 큰올콩이 Fukura보다 수량성이 높았다. 2. 남부지방의 산간 고랭지는 평야지에 비해 생육 및 100립종이 저하하고 수량성이 크게 떨어져 평야지보다 불리하였다. 3. 안전립 수량은 파종시기가 빠를수록 높고, 평야지 및 큰올콩품종에서 증수되었으며, 전체 립종에 대한 안전립 비율은 고랭지에서 높게 나타났다. 4. 파종시기에 따른 병해정도는 큰올콩은 뚜렷한 차이가 없었으나 Fukura는 파종기가 빠를수록 나병립율이 높았고, 미이라병은 파종기가 빠를수록 심하게 발생하였으며 자정병은 파종기가 늦을수록 많이 발생하는 경향이었다. 5. 종자의 발아율은 파종기가 늦은 것이 높았으며, 5월 15일에 파종하여 수확ㆍ저장한 종자의 발아율은 매우 낮아 문제시 되었다. 따라서 풋콩 종자생육을 위한 적정파종시기는 수파기의 고온ㆍ다습한 환경을 고려하여 6월 중순경이 좋을것으로 생각되었다.

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