• Journal of Embryo Transfer
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• v.25 no.2
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• pp.103-110
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• 2010

The first morphogenetic event of preimplantation development, compaction, was required efficient production of porcine embryos in vitro. Compaction of the porcine embryo, which takes place at post 4-cell stage, is dependent upon the adhesion molecule E-cadherin. The E-cadherin through ${\beta}$-catenin contributes to stable cell-cell adhesion. Rho-associated kinase (ROCK) signaling was found to support the integrity of E-cadherin based cell contacts. In this study, we traced the effects of ROCK-1 on early embryonic development and structural integrity of blastocysts in pigs. Then, in order to gain new insights into the process of compaction, we also examined whether ROCK-1 signaling is involved in the regulation of the compaction mediated by E-cadherin of cellular adhesion molecules. As a result, real-time RT-PCR analysis showed that the expression of ROCK-1 mRNA was presented throughout porcine preimplantation stages, but not expressed as consistent levels. Thus, we investigated the blastocyst formation of porcine embryos treated with LPA and Y27632. Blastocysts formation and their qualities in LPA treated group increased significantly compared to those in the Y27632-treated group (p < 0.05). Then, to determine whether ROCK-1 associates embryonic compaction, we explored the effect of activator and/or inhibitor of ROCK-1 on compaction of embryos in pigs. The rate of compacted morula in LPA treated group was increased compared to that in the Y27632-treated group (39.7 vs 12.0%). Furthermore, we investigated the localization and expression pattern of E-cadherin at 4-cell stage porcine embryos in both LPA- and Y27632-treated groups by immunocytochemical analysis and Western blot analysis. The expression of E-cadherin was increased in LPA-treated group compared to that in the Y27632-treated group. The localization of E-cadherin in LPA-treated group was enriched in part of blastomere contacts compared to that Y27632-treated group. ROCK-1 as a crucial mediator of embryo compaction may plays an important role in regulating compaction through E-cadherin of the cell adhesion during the porcine preimplantation embryo. We concluded that ROCK-1 gene may affect the developmental potential of porcine blastocysts through regulating embryonic compaction.

• Journal of the Society of Disaster Information
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• v.10 no.1
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• pp.33-39
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• 2014

Chemical accidents are the cause of the accident site during the initial responders to quickly and easily see materials and concentration method for the U.S. Environmental Protection Agency(EPA) is widely used in the initial response team direct reading detection equipment used. Ministry of the tubular gas detection equipment to detect direct reading detection equipment used in the event of an accident scene, and shell-and-tube gas detector for rapid detection and identification and precise analysis of causative pollutants before about strategically can identify the quantitative and qualitative useful equipment. However, those who initially respond to the scene of a direct reading detection equipment and a simple lack of understanding of how to use the numbers only because of the way you want to check the accuracy of detection results have been raising questions about the increase. The scene of the accident in order to obtain an accurate detection results used in this paper, the Ministry of Environment of gas detectors detect tubular Kitagawa and Draeger detector tube to check the reactivity of the material on-site detection of early response of those who were to raise the accuracy of the results.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.10 no.1
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• pp.59-66
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• 2010

Enzyme replacement of therapy (ERT) is one of the most promising therapeutic strategies for the treatment of lysosomal storage disorders. ERT is available in three types of Mucopolysaccharidosis (MPS): for MPS I (Aludrazyme$^{(R)}$), MPS II (Elaprase$^{(R)}$) and MPS VI (Naglazyme$^{(R)}$) patients who are over 5 years old. But recently, early diagnosis can be done by expert clinicians and even in prenatal case. We describe the case of ERT under 5 years old MPS patients. Up to June, 2010 in Samsung Medical Center, there are 6patients who were diagnosed as MPS and started ERT under 5 years old. 3 patients were MPS I, 3 patients were MPS II. 2 patient who was diagnosed as MPS I was female and others were male. Their age at diagnosis were 4 to 37month-old (4, 13, 16, 25, 27, 37 month-old) and they are now 9 to 60 month-old (9, 39, 32, 81, 60 month-old). The youngest patient was started ERT at 4 month-old and others were started at their 13 to 49 month-old (13, 29, 27, 28, 49 month-old). First manifested symptoms of patients were macrocephaly, kyphosis and coarse face appearance. Especially, in 2 of them, one was MPS I and the other was MPS II had elder brother with same disease. And the youngest one was diagnosed by the iduronate-2-sulfatase (IDS) gene analysis from chorionic villi sampling. His mother knew that she was a heterozygous carrier of IDS gene mutation because her younger brother died from MPS II. All of them confirmed as MPS by the enzyme assay in leukocytes and fibroblast skin culture. We started ERT with ${\alpha}$-L-iduronidase(Aldurazyme$^{(R)}$) to MPS I and did recombinant human iduronate-2-sulfatase (Elaprase$^{(R)}$) to MPS II patients as recommended dose as over 5 years old. But for MPS II patient who was 4 month old, we started ERT by recombinant human IDS (Elaprase$^{(R)}$) with reduced dose 0.1 mg/kg and increased dose every 2 weeks by 0.1mg/kg up to 0.5mg/kg IV infusion. During ERT, all patients had no adverse effects and the excretion of GAGs were decreased. We have evaluated other clinical symptoms such as liver/ spleen volume, heart function and neurologic evaluation. We describe a successful ERT to MPS I and MPS II patient under 5 years old without any adverse event. It indicates that ERT in young children are well tolerated and that it has several effects which may confer clinical benefits with long-term therapy.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.3-6
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• 2000

Antimicrobial resistance has been a well-recognized problem ever since the introduction of penicillin into clinical use. History of antimicrobial development can be categorized based on the major antibiotics that had been developed against emerging resistant $pathogens^1$. In the first period from 1940 to 1960, penicillin was a dominating antibiotic called as a "magic bullet", although S.aureus armed with penicillinase led antimicrobial era to the second period in 1960s and 1970s. The second stage was characterized by broad-spectrum penicillins and early generation cephalosporins. During this period, nosocomial infections due to gram-negative bacilli became more prevalent, while those caused by S.aureus declined. A variety of new antimicrobial agents with distinct mechanism of action including new generation cephalosporins, monobactams, carbapenems, ${\beta}$-lactamase inhibitors, and quinolones characterized the third period from 1980s to 1990s. However, extensive use of wide variety of antibiotics in the community and hospitals has fueled the crisis in emerging antimicrobial resistance. Newly appeared drug-resistant Streptococcus pneumoniae (DRSP), vancomycin-resistant enterococci (VRE), extended-spectrum ${\beta}$-lactamase-producing Klebsiella, and VRSA have posed a serious threat in many parts of the world. Given the recent epidemiology of antimicrobial resistance and its clinical impact, there is no greater challenge related to emerging infections than the emergence of antibiotic resistance. Problems of antimicrobial resistance can be amplified by the fact that resistant clones or genes can spread within or between the species as well as to geographically distant areas which leads to a global concern$^2$. Antimicrobial resistance is primarily generated and promoted by increased use of antimicrobial agents. Unfortunately, as many as 50 % of prescriptions for antibiotics are reported to be inappropriate$^3$. Injudicious use of antibiotics even for viral upper respiratory infections is a universal phenomenon in every part of the world. The use of large quantities of antibiotics in the animal health industry and farming is another major factor contributing to selection of antibiotic resistance. In addition to these background factors, the tremendous increase in the immunocompromised hosts, popular use of invasive medical interventions, and increase in travel and mixing of human populations are contributing to the resurgence and spread of antimicrobial resistance$^4$. Antimicrobial resistance has critical impact on modem medicine both in clinical and economic aspect. Patients with previously treatable infections may have fatal outcome due to therapeutic failure that is unusual event no more. The potential economic impact of antimicrobial resistance is actually uncountable. With the increase in the problems of resistant organisms in the 21st century, however, additional health care costs for this problem must be enormously increasing.

• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.4
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• pp.1-12
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• 2020

The extreme 2017 spring drought affected a large portion of South Korea in the Southern Gyeonggi-do and Chungcheongnam-do districts. This drought event was one of the climatologically driest spring seasons over the 1961-2016 period of record. It was characterized by exceptionally low reservoir water levels, with the average water level being 36% lower over most of western South Korea. In this study, we consider drought response methods to alleviate the shortage of agricultural water in times of drought. It could be to store water from a stream into a reservoir. There is a cyclical method for reusing water supplied from a reservoir into streams through drainage. We intended to present a decision-making plan for water supply based on the calculation of the quantity of water supply and leakage. We compared the rainfall-runoff equation with the TANK model, which is a long-term run-off model. Estimations of reservoir inflow during non-irrigation seasons applied to the Madun, Daesa, and Pungjeon reservoirs. We applied the run-off flow to the last 30 years of rainfall data to estimate reservoir storage. We calculated the available water in the river during the non-irrigation season. The daily average inflow from 2003 to 2018 was calculated from October to April. Simulation results show that an average of 67,000 tons of water is obtained during the non-irrigation season. The report shows that about 53,000 tons of water are available except during the winter season from December to February. The Madun Reservoir began in early October with a 10 percent storage rate. In the starting ratio, a simulated rate of 4 K, 6 K, and 8 K tons is predicted to be 44%, 50%, and 60%. We can estimate the amount of water needed and the timing of water pump operations during the non-irrigation season that focuses on fresh water reservoirs and improve decision making for efficient water supplies.

• Journal of Korea Water Resources Association
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• v.46 no.11
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• pp.1115-1128
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• 2013

The purpose of this study is to evaluate the suspended solids and eutrophication processes relationships in Chungju lake using CE-QUAL-W2, two-dimensional (2D) longitudinal/vertical hydrodynamic and water quality model. For water quality modeling, the lake segmentation was configured as 7 branches system according to their shape and tributary distribution. The model was calibrated (2010) and validated (2008) using 2 years of field data of water temperature, suspended solids (SS), total nitrogen (TN), total phosphorus (TP) and algae (Chl-a). The water temperature began to increase in depth from April and the stratification occurred at about 10 m early July heavy rain. The high SS concentration of the interflow density currents entering from the watershed was well simulated especially for July 2008 heavy rainfall event. The simulated concentration range of TN and TP was acceptable, but the errors might occur form the poor reflection for sedimentation velocity of nitrogen component and adsorption-sediment of phosphorus in model. The concentration of Chl-a was simulated well with the algal growth patterns in summer of 2010 and 2008, but the error of under estimation may come from the use of width-averaged velocity and concentration, not considering the actual to one side inclination by wind effect.

• Journal of Korean Association for Spatial Structures
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• v.7 no.2 s.24
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• pp.105-114
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• 2007

Large span spaces have been widely used for various purposes including sports events in other countries. Due to increasing demands on multi-purpose use of such spaces, recently built large span sport facilities such dome stadiums have been required to accommodate sport events as well as performing events. Also, retractable ceilings and/or walls were generally adopted in those spaces for providing various event conditions. It seems obvious that the openings between diffuse fields and free field may cause difficulties in the acoustic design of such spaces In the present work, the acoustic characteristics of non-diffuse field has been investigated using 1/10 acoustic scale models. It was found that RTs at low- and mid-frequencies decayed faster than those at high-frequencies as the percentage of opening area increased. The decay rate of RTs at high frequencies were not influenced by increasing the area of an opening. Also high dependence of EDT on the percentage of opening area was observed at all frequency range. D50 was not improved by increasing the area of an opening up to 12.5% and then sharply increased. The application of Schroeder integration to evaluate the reverberation charateristics of coupled spaces may not be proper, since non-exponential decay process with double or triple decays of those spaces can not be properly defined. EDT seems more appropriate to predict reverberation at the early acoustic design stage of acoustically coupled spaces.

• Journal of the Korean Geographical Society
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• v.33 no.3
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• pp.447-454
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• 1998

As a widespread tephra erupted 24 to 25 KA, Aira-Tn Ash, or AT, provides an invaluable datum for stratigraphic study of late Pleistocene in Northeast Asia. AT can be identified from their tonal characteristic and morphology (the so­called bubble-wail feature), as well as typical refractive index. First reported in 1983, its presence was confirmed from many palaeolithic deposits distributed throughout Korea. It forces us to reconsider the age of prominent Pleistocene deposits. At the famous archaeological site of Chon-gok-ni, presence of AT lends a firm support that sedimentation and deposition of lithic assemblage were in fact a late Upper Pleistocene event, thus, throwing a new question at archaeologists to explain why such archaic-looking tools were continued to be made. Discovery of AT at Chongdongjin is more surprising, since the coastal terrace has been regarded by many as an early Lower Pleistocene eustatic feature. It implies strongly that we need to devise a new scheme for Quaternary stratigraphy. In the absence of reliable datum and marker for Quaternary research, efforts must be given to search for and identify AT and other widespread tephras.

• Spatial Information Research
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• v.22 no.3
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• pp.71-77
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• 2014

There are numbers of unpredictable risk factors in the disaster scenes such as fire, explosion and fail to early life-saving or holding the flames which can lead to massive damage. In particular, fire-fighters who arrive on the scene within 5 minutes after dispatching, have a limitation to get aware to the situation of scene fully, because of immediate deploy to disaster scene with limited information. This situation may lead to disturbance that fire-fighters perform effective fire-fighting activities, to put fire-fighter's life at risk by misjudge the situation. Previous domestic and International studies focused vulnerability for spatial area or features which can damage to life and property in the event of anticipated. In this study, we have been developed fire-fighting vulnerable zone model that can analyze comprehensively hindrance factors for fire-fighting activities targeting whole life cycle of fire-fighting activities from dispatch to fire suppression or life-saving. In addition, we have been given shape to finality and applicability for our model by defining the new concept of fire-fighting vulnerable zone which can be distinguished from the concept of fire vulnerable area in previous studies. The results of this study can be used to analysis fire-fighting vulnerable zone type analysis, establish fire-fighting policies and improve the performance of decision-making process.

• Journal of Convergence for Information Technology
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• v.7 no.5
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• pp.1-13
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• 2017

It is revealed that there are more registered disabled people in our country who have their disability because of acquired factors such as accidents than because of innate deformity. This phenomenon will continue and the incidence of acquired disability will increase more and more. Even though there is noticeable change, the social recognition toward the disabled is still negative. Particularly, university students are in the early adulthood according to the development of life-cycle, and this is the period that people have diverse social relationships, depart from unified frame and work in various fields, and set up their own thoughts and ideology with knowledge and skills acquired from university education. Therefore, in this study, we are going to search the recognition of the university students, who are in the previous period of entering into the society, toward the disabled, and if they have negative prejudice or attitude against the disabled, we are going to find the ways to improve on their awareness positively. The subjects of the survey were randomly selected among 230 out of total 250 students by visiting thirteen universities in Chungcheong Province, and were analyzed by using SPSS (ver. 18.0) program. T-test and One-way ANOVA were used as analytical methods to look into the difference of analysis of frequency, descriptive statistic, reliability analysis and attitudes for comprehending sociodemographic characteristics of the subjects of the survey. In conclusion, it has to be not a temporary or event-like training, but a training that makes people have positive recognition and attitude towards accurate information, knowledge, human rights, disability, and diversity. This thesis has a limitation to be generalized to the university students all over the country since the region is limited to a certain area.