• Journal of Life Science
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• v.16 no.4
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• pp.626-631
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• 2006

This study was carried out to investigate the physical characteristics (height, body lenght, chest depth, head type, ear type, body color, eye type and tail type) and genetic diversity using 15 microsatellite DNA markers (PEZ 1, 5, 8, 10, 11, 12, 13, 15, 16, 17, 20, 21, FHC 2010, FHC 2054 and FHC 2079) in 44 random Miryang native dogs(6 months${\sim}$12 years old). The height, body lenght, and chest depth of Miryang native dogs were 43-55 cm(mean 49.5 cm), 45-60 cm(mean 54.3 cm), and 50-64 cm(mean 57.9 cm), respectively. Miryang native dog was medium sized. The head and eye type were reverse-triangle(100%), triangle (90.9%) and newborn moon(9.1%), respectively. Most of body color had white coat color(93.2%), light pink tongue color(100%), light black anal color(90,9%) and pink claw color(100%). The ear type showed erect ear(100%), and half-curled(56.8%), upward(34.1%), curled(9.1%) in tail type, respectively. Number of alleles observed at a single locus ranged from 2 (PEZ 21 and FHC 2010) to 14 (PEZ 13), with average number of alleles per locus of 6.13. The expected heterozygosities of 15 microsatellite loci were estimated based on gene frequencies. The highest expected heterozygosity, 0.863 was estimated in PEZ 13 locus and the lowest, 0.455 in PEZ 21 and FHC 2010 locus. And the mean expected heterozygosity of 15 microsatellite markers was calculated as 0.635. Polymorphic information content (PIC) values were ranged from 0.348 (PEZ 21 and FHC 2010) to 0.837 (PEZ 13), and the mean PIC value was calculated as 0.570. Of the 15 markers, PEZ 10, PEZ 13, PEZ 17 and FHC 2054 loci have relatively high PIC value (> 0.7) in Miryang native dog. In order to determine the efficieney of parentage control, exclusion probabilities (PE) were calculated for each allele. The highest PE 1 and PE 2 in PEZ 13 locus was caculated to 0.548 and 0.710, respectively. And the total exclusion power in PE 1 and PE 2 was calculated to 0.9895 and 0.9996, respectively. These results can give basic information for perservation and research in Miryang native dog, and phylogenetic relationships of the Korean native dog and Asian dog breeds.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.1
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• pp.111-116
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• 2002

This report describes an inhibitory effect of Tongku-tang(TKT) on mast cell-mediated immediate-type allergic reactions. TKT is an Oriental herbal prescription, which has been successfully applied for the treatment of allergic disorders, mainly skin anaphylactic diseases in eastern medicine. TKT has concentration-dependently inhibited the ear swelling response induced by intradermal injection of non-specific mast cell degranulator compound 48/80 in mice. TKT also inhibited mast cell-dependent passive cutaneous anaphylaxis activated by dinitrophenyl (DNP)-IgE antibody in rats. I studied the effect of TKT on the histamine and β-hexosaminase release from the rat peritoneal mast cells by compound 48/80. TKT did not inhibit significantly the histamine and β-hexosaminase release from the rat peritoneal mast cells by compound 48/80. However, TKT inhibited both TNF-α and IL-1β secretion induced by phorbol 12-myristate 13-acetate and A23187 respectively. These results provide evidence that TKT may be beneficial in the treatment of immediate-type allergic reaction.

• Korean Journal of Pharmacognosy
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• v.50 no.4
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• pp.277-284
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• 2019

Rumex crispus is known to have anticancer, antioxidant, antibacterial, and bone loss inhibitory activities. Mast cells are critical immune cells that induce a type 1 IgE-mediated allergic reaction. However, there are no reports of inhibitory effects of Rumex crispus on mast cells and allergic reactions. In this study, we performed some experiments to investigate whether Rumex crispus ethanol extract(RCE) has any inhibitory effect on antigen-induced type I allergic response in vitro and in vivo. RCE inhibited degranulation of IgE-mediated mast cells(IC₅₀, ~57 ㎍/ml) and cytokine production such as TNF-α and IL-4 in a dose-dependent manner. In vivo, RCE significantly inhibited passive cutaneous anaphylaxis(PCA)(ED₅₀, ~198 mg/kg) in mice. Furthermore, RCE inhibited degranulation of MCs in ear tissue of mice with PCA. Mechanism studies showed that RCE inhibited the activation of Syk and Syk-dependent pathway such as LAT, PLC-γ, Akt, and MAP Kinase. Our results demonstrate for the first time that RCE inhibits type I hypersensitive response by suppressing the activity of Syk in mast cells, thereby reducing degranulation and cytokine production. Taken together, RCE could be used as a novel therapeutic material to suppress allergic diseases.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.2
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• pp.391-401
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• 1995

Several tests of the Double Cantilever Beam(DCB) were carried out for influence of the fiber orientation on the Mode I of the interlaminar fracture behavior in the Carbon/Epoxy composites. The interlaminar fracture toughness of Mode I was estimated based on the energy release rate of Mode I, $G_{I}$. The fracture toughness at crack initiation, $G_{IC}$, increases from type A to type E. The fracture toughness, $G_{IR}$ , is almost constant macroscopically for type A and type E when crack propagates. $G_{IR}$ for types B, C, D increases rapidly at the beginning of the crack growth then it decreases gradually. The fracture surface observation by SEM was also obtained the same results. Consequently the influence of the fiber orientation on the Mode I Interlaminar fracture behavior was made clear.ear.

• Archives of Plastic Surgery
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• v.34 no.4
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• pp.413-419
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• 2007

Purpose: In this study, porous type I collagen scaffolds were cross-linked using dehydrothermal(DHT) treatment and/or 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide(EDC), in the presence and absence of chondroitin-6-sulfate(CS) and cultured autologous chondrocytes(Chondro) for cartilage regeneration. Methods: Cartilage defects were created in the proximal part of the ear of New Zealand rabbits. Four prepared types of scaffolds(n=4) were inserted. The groups included Chondro-Collagen-DHT(Group 1), Chondro- Collagen-DHT-EDC(Group 2), Chondro-CS-Collagen- DHT(Group 3), and Chondro-CS-Collagen-DHT-EDC (Group 4). Histomorphometric analysis and cartilage-specific gene expression of the reconstructed tissues were evaluated 4, 8, and 12 weeks after implantation. Results: EDC cross-linked groups 2 and 4 regenerated more cartilage than other groups. However, calcification was observed in the 4th week after implantation. CS did not increase chondrogenesis in all groups. Cartilage-specific type II collagen mRNA expression increased in the course of time in all groups.Conclusion: EDC cross-linking methods maintain the scaffold and promote extracellular matrix production of chondrocytes.

• Journal of the Korean Society of Clothing and Textiles
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• v.15 no.4 s.40
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• pp.341-349
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• 1991

This study was done to provide basic data for clothing design and thus to contribute to the academic development of the clothing and textile area. The focus of this study was to charac­terize the somatotype based on each individual's lateral view. For this purpose, firstly, categorization of the subjects based on their lateral view and definition of each category, secondly, characterization of each somatotype, and thirdly, sugges­tion of the deterministic criteria of each category were complished. The subjects in this study were female college students of 18 to 26 year-old whose somatotypes were comparatively invariable after cessation of physical growth. The metrical items characterizing upper body lateral view were chosen. Data were collected through Anthropometry and Photometry and analyzed by Factor analysis. The results were as follows; 1. Each subject's side view contour was classified as straight type, lean-back type, bend­forward type, and swayback according to its position to the relative plumb line. Straight type was defined as the type in which the plumb line passes through the lobe of the ear, the shoulder joint, and the mid abdominal region laterally. Lean-back type positioned the plumb line more posteriorly than straight type. Bend-forward type positioned the plumb line more anteriorly than straight type. Swayback positioned the plumb line at about the same line as straight type. And curvature of side view contur was more prominent in this type than in straight type. 2. Seven factors were figured out. The first factor was representing upperbody volume, and the second factor was representing size, the third factor was horizontal distance from lateral view representing size view contour. The fourth factor was front body length, the fifth factor was back body length. The sixth factor was shoulder length and shoulder width representing shoulder shape. The seventh factor was the bust shape.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.312-318
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• 2014

We evaluated the morphological characteristics of 156 maize inbred lines, which were developed to breeding normal maize variety at Maize Experiment Station, Gangwon Agricultural Research and Extension Services, by examining 11 quantitative and three qualitative characteristics. On the evaluation of three qualitative traits for 156 maize inbred lines, most inbred lines showed yellow (85 and 84 inbred lines) at tassel color (QL1) and silk color (QL2), and showed semi erect (105 inbred lines) at plant type (QL3). While, the evaluation of 11 quantitative traits for 156 maize inbred lines, they showed the morphological variation in days of tasseling (QN1, 56.5 to 76.0 days), days of silking (QN2, 59.0 to 85.5 days), stem thickness (QN3, 12.7 to 42.9 mm), plant height (QN4, 111.8 to 239.8 cm), ear height (QN5, 48.2 to 126.5 cm), 100 kernel weight (QN6, 14.9 to 36.4 g), ear length (QN7, 10.0 to 79.0 cm), setted kernel length (QN8, 8.0 to 70.5 cm), ear thickness (QN9, 4.0 to 22.0 cm), total kernel weight (QN10, 22.0 to 490.0 kg) and water content (QN11, 9.3 to 11.9%), respectively. As a result, 11 inbred lines (00hf3, 00hf19, 00hf30, 00hf36, 02S8069, 02S8072, 02S8090, 02S8099, 05S10011, 06S8085-6, 07S8011) in the 156 normal maize inbred lines have showed comparatively high values. While, the results of PCA (principal component analysis) indicated that the ear length (QN7), setted kernel length (QN8), ear thickness (QN9) and total kernel weight (QN10) greatly contributed in positive direction on the first principal components. And also, days of tasseling (QN1), days of silking (QN2), plant height (QN4) and ear height (QN5) contributed in negative direction on the second principal component. Thus these morphological characters, which were greatly contributed in the first and second principal components, might be considered to be useful for discrimination among 156 normal inbred lines. Specifically, this study's assessment of morphological characteristics of 156 normal inbred lines will be helpful useful for normal maize breeding programs such activities as planning crosses for hybrid and line development at Maize Experiment Station, Gangwon Agricultural Research and Extension Services.

• Journal of Environmental Science International
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• v.24 no.2
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• pp.261-266
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• 2015

We have analyzed the external morphology and the environment of the foraging site of Vespertilio sinensis. The external morphology was analyzed by twelve parameters and the environment characteristics of the foraging site was analyzed using GIS 10.1 program. The wing membrane was inserted into the ankle of the hind foot and the wing ratio was 1.42, the middle type between broad-short wing type and long-narrow wing type. The fur color was blackish brown but the guard hair color was whitish. The shape of the ear was a rounded triangle and tragus was a fan shape. This study showed that V. sinensis preferred the deciduous forest of the upper forest zone, where human interference was less. We believed that abundance of insects, depending on water system, was closely related to the use of the foraging site. This result showed that the environment characteristics was very similar to the nature preservation zone including Baekyang valley and Keumsun valley in Naejangsan National Park.

• Journal of architectural history
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• v.21 no.3
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• pp.45-54
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• 2012

This research work is to analyze architectural characteristics of Sungnyemun especially in King Yeongjo's reign in Joseon dynasty. The result of this research is summarized as following: 1. The architectural characteristics of Sungnyemun in King Yeongjo's reign are closely related with Confucian ceremonies such as Jeon-jwa and Heon-goek-rye. To perform these ceremonies, some lower walls of Sungnyemun's wooden pavilion were removed and used as ceremonial space. And after ceremony it was restored. 2. The floor type of center bay of the 1st story of wooden pavilion should have a type of floor using long and narrow fine tree plate, which is same type before the repair work of 1960's dismantlement. 3. The width of east stairway which is reached to east small gate, was changed just before Japanese's rule(1910~1945), should be broaden than present width, which is proven through the recent excavation. 4. The reason of asymmetric characteristic of locations of both east and west narrow-gate, and widths of east and west stairway, are related with order of King's ceremony. * Jeonjwa : a ceremony to see national affairs or receive royalty from officials in main hall or main gate of palace in Joseon dynasty (some times open to public) * Heon-goek-rye : a ceremony after win a war and offering to king enemy's ear or head in Joseon dynasty.

• Korean Journal of Pharmacognosy
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• v.50 no.3
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• pp.175-184
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• 2019

Mast cells are crucial as effector cells in the immediate-type allergic reaction. Lentinus edodes has been the popular edible mushroom in oriental countries and reported to have immunomodulatory, anti-tumor, anti-atherogenic, anti-viral, and anti-allergic activities. However, the roles of L. edodes in mast cell-mediated anaphylactic reaction have not been fully elucidated. In this research, we have demonstrated the effects of the methanol extract of L. edodes (MELE) on mast cell-mediated anaphylaxis-like and anaphylactic reactions. MELE suppressed systemic anaphylaxis-like reaction, plasma histamine levels, and ear swelling response in mice treated with compound 48/80. MELE also suppressed passive systemic and cutaneous anaphylaxis mediated by anti-dinitrophenyl IgE. In accordance with these findings, MELE dose-dependently decreased histamine release from RPMC evoked by compound 48/80 or the antigen-antibody reaction. To clarify the mechanism of degranulation system, intracellular cAMP levels as well as calcium influx in RPMC was evaluated. In compound 48/80-treated RPMC, MELE blocked calcium uptake into the cells. In addition, MELE elevated the intracellular cAMP content and significantly attenuated compound 48/80-induced cAMP reduction in RPMC. Taken together, we propose the clinical use of MELE in mast cell-mediated immediate-type allergic diseases.