Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Effect of cultured chondrocyte-seeded chondroitin-sulfate conjugated type I collagen scaffold on cartilage regeneration

콘드로이틴 환산염을 결합한 I형 콜라겐지지체와 연골세포를 이용한 연골재생

  • Lim, Joong Jae (Duo Plastic Surgery Clinic) ;
  • Son, Daegu (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center) ;
  • Son, Kyounghee (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center) ;
  • Yang, Eunkyung (Bioland) ;
  • Han, Kihwan (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center)
  • 임중재 (듀오 성형외과) ;
  • 손대구 (계명대학교 의과대학 성형외과학교실) ;
  • 손경희 (계명대학교 의과대학 성형외과학교실) ;
  • 양은경 (바이오랜드) ;
  • 한기환 (계명대학교 의과대학 성형외과학교실)
  • Received : 2006.11.13
  • Published : 2007.07.10
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Abstract

Purpose: In this study, porous type I collagen scaffolds were cross-linked using dehydrothermal(DHT) treatment and/or 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide(EDC), in the presence and absence of chondroitin-6-sulfate(CS) and cultured autologous chondrocytes(Chondro) for cartilage regeneration. Methods: Cartilage defects were created in the proximal part of the ear of New Zealand rabbits. Four prepared types of scaffolds(n=4) were inserted. The groups included Chondro-Collagen-DHT(Group 1), Chondro- Collagen-DHT-EDC(Group 2), Chondro-CS-Collagen- DHT(Group 3), and Chondro-CS-Collagen-DHT-EDC (Group 4). Histomorphometric analysis and cartilage-specific gene expression of the reconstructed tissues were evaluated 4, 8, and 12 weeks after implantation. Results: EDC cross-linked groups 2 and 4 regenerated more cartilage than other groups. However, calcification was observed in the 4th week after implantation. CS did not increase chondrogenesis in all groups. Cartilage-specific type II collagen mRNA expression increased in the course of time in all groups.Conclusion: EDC cross-linking methods maintain the scaffold and promote extracellular matrix production of chondrocytes.

Keywords

References

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