Browse > Article

Effect of cultured chondrocyte-seeded chondroitin-sulfate conjugated type I collagen scaffold on cartilage regeneration  

Lim, Joong Jae (Duo Plastic Surgery Clinic)
Son, Daegu (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center)
Son, Kyounghee (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center)
Yang, Eunkyung (Bioland)
Han, Kihwan (Department of Plastic and Reconstructive Surgery, Keimyung University, Dongsan Medical Center)
Publication Information
Archives of Plastic Surgery / v.34, no.4, 2007 , pp. 413-419 More about this Journal
Abstract
Purpose: In this study, porous type I collagen scaffolds were cross-linked using dehydrothermal(DHT) treatment and/or 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide(EDC), in the presence and absence of chondroitin-6-sulfate(CS) and cultured autologous chondrocytes(Chondro) for cartilage regeneration. Methods: Cartilage defects were created in the proximal part of the ear of New Zealand rabbits. Four prepared types of scaffolds(n=4) were inserted. The groups included Chondro-Collagen-DHT(Group 1), Chondro- Collagen-DHT-EDC(Group 2), Chondro-CS-Collagen- DHT(Group 3), and Chondro-CS-Collagen-DHT-EDC (Group 4). Histomorphometric analysis and cartilage-specific gene expression of the reconstructed tissues were evaluated 4, 8, and 12 weeks after implantation. Results: EDC cross-linked groups 2 and 4 regenerated more cartilage than other groups. However, calcification was observed in the 4th week after implantation. CS did not increase chondrogenesis in all groups. Cartilage-specific type II collagen mRNA expression increased in the course of time in all groups.Conclusion: EDC cross-linking methods maintain the scaffold and promote extracellular matrix production of chondrocytes.
Keywords
Collagen type I; Chondrocytes; Chondroitin sulfates;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Son DG, Lim JJ, Sohn KH, Yang EK: The effect of various methods of cross-linking in type I collagen scaffold on cartilage regeneration. J Korean Soc Plast Reconstr Surg 33: 723, 2006
2 Freed LE, Hollander AP, Martin I, Barry JR, Langer R, Vunjak-Novakovic G: Chondrogenesis in a cell-polymer-bioreactor system. Exp Cell Res 240: 58, 1998   DOI   ScienceOn
3 Rhie JW, Ahn TJ, Park JG, Sohn JY, Cho HS, Lim P: Culture of chondrocytes on scaffolds with different pore network of PLGA and PLLA. J Korean Soc Plast Reconstr Surg 30: 237, 2003
4 van Susante JL, Buma P, Schuman L, Homminga GN, van den Berg WB, Veth RP: Resurfacing potential of heterologous chondrocytes suspended in fibrin glue in large full-thickness defects of femoral articular cartilage: an experimental study in the goat. Biomaterials 20: 1167, 1999   DOI   ScienceOn
5 Pieper JS, Oosterhof A, Dijkstra PJ, Veerkamp JH, van Kuppevelt TH: Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate. Biomaterials 20: 847, 1999   DOI   ScienceOn
6 Honorati MC Cattini L, Facchini A: IL-17, IL1-beta and TNFalfa stimulate VEGF production by dedifferentiated chondrocytes. Osteoarthritis Cartilage 12: 683, 2004   DOI   ScienceOn
7 Grigolo B, Roseti L, Fiorini M, Fini M, Giavaresi G, Aldini NN, Giardino R, Facchini A: Transplantation of chondrocytes seeded on a hyaluronan derivative (Hyaff$^{\circledR}$-11) into cartilage defects in rabbits. Biomaierials 22: 2417, 2001   DOI   ScienceOn
8 Hauselmann HJ, Masuda K, Hunziker EB, Neidhart M, Mok SS, Michel BA, Thonar EJ: Adult human chondrocytes cultured in alginate from a matrix similar to native human articular cartilage. Am J Physiol 271: C742, 1996   DOI
9 van Susante JL, Pieper J, Buma P, van Kuppevelt TH, van Beuningen H, van Der Kraan PM, Veerkamp JH, van den Berg WB, Veth RPH: Linkage of chondroitin-Sulfate to type I collagen scaffolds stimulates the bioactivity of seeded chondrocytes in vitro. Biomaieriale 22: 2359, 2001
10 Breinan H, Minas T, Hsu HP, Nehrer S, Sledge CB, Spector M: Effect of cultured autologous chondrocytes on repair of chondral defects in a canine model. J Bone Jt Surg 79-A: 1439, 1997
11 Kim WS, Vacanti JP, Cima L, Mooney D, Upton J, Puelacher WC, Vacanti CA: Cartilage engineered in predetermined shapes employing cell transplantation on synthetic biodegradable polymers. Plast Reconstr Surg 94: 233, 1994   DOI   ScienceOn
12 Chvapil M, Kronenthal L, van Winkle W: Medical and surgical applications of collagen. Int Rev Connect Tissue Res 6: 1, 1973
13 Pachence JM: Collagen-based devices for soft tissue repair. J Biomed Mater Res 33: 35, 1996   DOI
14 Aigner J, Tegeler J, Hutzler P, Campoccia D, Pavesio A, Hammer C, Kastenbauer E, Naumann A: Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester. J Biomed Master Res 42: 172, 1998   DOI   ScienceOn
15 Lee CR, Grodzinsky AJ, Hsu HP, Spector M: Effects of a cultured autologous chondrocyte-seeded type II collagen scaffold on the healing of a chondral defect in a canine model. J Orthopaedic Research 21: 272, 2003   DOI   ScienceOn