• Korean Journal of Plant Tissue Culture
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• v.28 no.5
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• pp.283-287
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• 2001

Differential display based on PCR was employed to identify genes expressed during tuber-developing stage of potato (Solanum tuberosum L. cv. Irish Cobbler). An eukaryotic initiation factor 5A (eIF-5A) clone isolated from a cDNA library constructed with developing micro-tuber using a probe of PCR fragment. We isolated three positive clones and ore of them contained open reading frame. This clone revealed high sequence similarity to tomato eIF 5A cDNA. At the DNA level, there is 94.8% identity with the tomato eIF-5A4, whereas at the protein level there is a high identity with 97.5%. The potato eIF 5A clone is 716 bp in length and contains a single open reading frame from 57 to 539 bp, a 56 bp 5'-untranslated region and a 177 bp 3'-untranslated region. The deduced protein composed of 160 amino acid residues, with a predicted molecular mass of 17.4 kD and an estimated pl of 5.5. The sequence of 12 (STSKTGKHGHAK) amino acids among eIF-5A proteins is perfectly conserved from yeast to human. That sequence in potato eIF-5A protein is also conserved at position 46 to 57 amino acid. This region embeds the post-translational modification site of the lysine residue (at the seventh K) to hypusine that is crucial to eIF-5A activity. The northern blot analysis of eIF5A has shown abundant expression, mainly in flower organs (stamen, ovary, petal, sepal), fruit and stolen.

• Korean Journal of Microbiology
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• v.40 no.1
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• pp.7-11
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• 2004

eIF5B is a translation initiation factor that delivers Met-$tRNA^{Met}$ to AUG start codon and subsequently joins the small and large ribosomes. In order to study the function of eIF5B encoded by FUN12, we constructed FUN12 which lacked 5' end of its sequence. We found that this construct lacking almost all of its promoter in pRS plasmid partially complemented slow growth phenotype of fun12 deletion strain. Interestingly, this construct expressed N-terminally truncated eIF5B and its expression level was about 5% of that of wild type eIF5B. Low amount of the eIF5B expressed additionally in fun12 deletion strain played a direct role as a limiting factor for its growth. This limiting factor eIF5B in those strains also modulates activities of overall translation in vitro.

• Journal of Life Science
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• v.23 no.7
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• pp.941-946
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• 2013

Local protein synthesis at subsynaptic sites plays a key role in the regulation of the protein composition in local domains. In this study, we carried out immunocytochemistry of cultured rat hippocampal neurons in various developmental stages to investigate the expression of eIF4E and its binding protein, eIF4EBP1. Both proteins were distributed in dendrites. In addition, eIF4EBP1 was highly expressed in the nucleus throughout the development, whereas eIF4E was not expressed in the nucleus. Punctate expression of eIF4E and eIF4EBP1 was evident in DIV 3. The colocalization rates of eIF4E or eIF4EBP1 puncta with PSD95 were higher in the dendrogenic than in the mature stages. In contrast, the colocalization rates of eIF4E and eIF4EBP1 puncta were higher in the mature than in the dendrogenic stages. As eIF4E is inactive when it is bound to eIF4EBP1, these data indicate that most dendritic eIF4E's are active during development but that they are mostly under inhibition in mature neurons.

• Journal of Life Science
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• v.21 no.11
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• pp.1526-1531
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• 2011

Local protein synthesis in neuronal dendrites is important for site-specific regulation of synaptic plasticity. In this study, we investigated whether translation initiation factors (eIFs) are present at the postsynaptic sites. High resolution confocal microscopy showed that the eIF4E and eIF4G (which bind the 5'-terminal mRNA cap), eIF5 (which is important during the 3' direction scanning to find an initiation codon), eIF6 (which mediates upregulation of translation by external stimuli), and eIF5A (which mediate translation upregulation under adverse conditions) were localized to the post-synaptic sites. Immunoblot and detergent extraction experiments also indicated that these eIFs were present in the synapse in association with the postsynaptic density (PSD). Our data provide evidence for the strategic positioning of eIFs at the postsynaptic site for initiation of translation in diverse situations.

• Molecules and Cells
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• v.25 no.2
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• pp.172-177
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• 2008

Eukaryotic translation initiation factor 5B (eIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, and promotes joining of the 60S ribosomal subunit. To see whether the eIF5B proteins of other organisms function in Saccharomyces cerevisiae, we cloned the corresponding genes from Oryza sativa, Arabidopsis thaliana, Aspergillus nidulans and Candida albican and expressed them under the control of the galactose-inducible GAL promoter in the $fun12{\Delta}$ strain of Saccharomyces cerevisiae. Expression of Candida albicans eIF5B complemented the slow-growth phenotype of the $fun12{\Delta}$ strain, but that of Aspergillus nidulance did not, despite the fact that its protein was expressed better than that of Candida albicans. The Arabidopsis thaliana protein was also not functional in Saccharomyces. These results reveal that the eIF5B in Candida albicans has a close functional relationship with that of Sacharomyces cerevisiae, as also shown by a phylogenetic analysis based on the amino acid sequences of the eIF5Bs.

• Journal of Advanced Navigation Technology
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• v.13 no.3
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• pp.319-326
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• 2009

In this paper, the key technologies of Navigation receiver for GNSS sensor station are presented as a development result of a GNSS ground station in ETRI. A wide-band antenna and RF/IF components and SW signal processing unit to cover the GPS and Galileo signals for GNSS receiver are developed and its performance is verified by using GPS live signal and GNSS RF signal simulator from SpirentTM. We also gather GIOVE-A signal by using H/W antenna and RF/IF units in IF-level as sampling frequency and bit number, 112MHz and 8bits, respectively by using the developed wide-band antenna and RF/IF components. Data acquisition is done by using commercial data acquisition device from National Instrument TM. The gathered data is fed into SW receiver to process Galileo E1 to verify Galileo signal processing by Galileo live signal from GIOVE-A.

• Journal of Satellite, Information and Communications
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• v.4 no.1
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• pp.36-44
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• 2009

The key technologies of GNSS receiver for GNSS sensor station are under development as a part of a GNSS ground station in ETRI. This paper presents the GNSS receiver implementation and signal processing result which is implemented based on FPGA to process the Galileo E1 and E5 signal. To verify the working and performance for GNSS receiver which is implemented based on FPGA, live signal received from GIOVE-B which is second test satellite is used. We gather GIOVE-B signal by using prototyping antenna and RF/IF units including IF-component. To verify Galileo E1 and E5 signal processing function from GIOVE-B, FPGA based signal processing module is implemented as a prototyping hardware board.

• Bulletin of the Korean Mathematical Society
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• v.48 no.5
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• pp.1033-1039
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• 2011

Let R be a ring with identity 1, I(R) be the set of all idempotents in R and G be the group of all units of R. In this paper, we show that for any semiperfect ring R in which 2 = 1+1 is a unit, I(R) is closed under multiplication if and only if R is a direct sum of local rings if and only if the set of all minimal idempotents in R is closed under multiplication and eGe is contained in the group of units of eRe. In particular, for a left Artinian ring in which 2 is a unit, R is a direct sum of local rings if and only if the set of all minimal idempotents in R is closed under multiplication.

• Journal of applied mathematics & informatics
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• v.29 no.5_6
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• pp.1117-1127
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• 2011

In this paper, a class of more general delayed viral infection model with lytic immune response is proposed by Song et al.[1] ([Journal of Mathematical Analysis Application 373 (2011), 345-355). We derive the basic reproduction numbers $R_0$ and $R_0^*$ 0 for the viral infection, and establish that the global dynamics are completely determined by the values of $R_0$ and $R_0^*$. If $R_0{\leq}1$, the viral-free equilibrium $E_0$ is globally asymptotically stable; if $R_0^*{\leq}1$ < $R_0$, the immune-free equilibrium $E_1$ is globally asymptotically stable; if $R_0^*$ > 1, the chronic-infection equilibrium $E_2$ is globally asymptotically stable by using the method of Lyapunov function.

• Bulletin of the Korean Mathematical Society
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• v.40 no.2
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• pp.223-234
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• 2003

This note contains the following results for a ring A : (1) A is simple Artinian if and only if A is a prime right YJ-injective, right and left V-ring with a maximal right annihilator ; (2) if A is a left quasi-duo ring with Jacobson radical J such that $_{A}$A/J is p-injective, then the ring A/J is strongly regular ; (3) A is von Neumann regular with non-zero socle if and only if A is a left p.p.ring containing a finitely generated p-injective maximal left ideal satisfying the following condition : if e is an idempotent in A, then eA is a minimal right ideal if and only if Ae is a minimal left ideal ; (4) If A is left non-singular, left YJ-injective such that each maximal left ideal of A is either injective or a two-sided ideal of A, then A is either left self-injective regular or strongly regular : (5) A is left continuous regular if and only if A is right p-injective such that for every cyclic left A-module M, $_{A}$M/Z(M) is projective. ((5) remains valid if 《continuous》 is replaced by 《self-injective》 and 《cyclic》 is replaced by 《finitely generated》. Finally, we have the following two equivalent properties for A to be von Neumann regula. : (a) A is left non-singular such that every finitely generated left ideal is the left annihilator of an element of A and every principal right ideal of A is the right annihilator of an element of A ; (b) Change 《left non-singular》 into 《right non-singular》in (a).(a).