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Species-Specific Duplex PCR for Detecting the Important Fish Pathogens Vibrio anguillarum and Edwardsiella tarda

  • Jo, Geon-A;Kwon, Sae-Bom;Kim, Na-Kyeong;Hossain, Muhammad Tofazzal;Kim, Yu-Ri;Kim, Eun-Young;Kong, In-Soo
    • Fisheries and Aquatic Sciences
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    • 제16권4호
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    • pp.273-277
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    • 2013
  • Vibriosis caused by Vibrio anguillarum and edwardsiellosis caused by Edwardsiella tarda are septicemic diseases of many commercially important freshwater and marine fishes, and threaten the aquaculture industry in Korea. Early diagnosis and accurate identification of these two bacterial species could help to prevent these diseases and minimize the damage to cultured marine species. This study designed a duplex polymerase chain reaction (PCR) method for the simultaneous detection of two major fish pathogens: V. anguillarum and E. tarda. Each pair of oligonucleotide primers exclusively amplified the target groEL gene of the specific microorganism. Twenty-two Vibrio and ten non-Vibrio enteric species were used to check the specificity of the primers, which were found to be highly specific for the target species, even among closely related species. The detection limit was 400 pg for V. anguillarum and 4 ng for E. tarda when mixed purified DNA was used as the template. This assay showed high specificity and sensitivity in the simultaneous detection of V. anguillarum and E. tarda from artificially inoculated seawater and fish.

이메일기록관리를 위한 메타데이터 표준요소선정에 관한 연구 (A Study on Standardized Metadata Elements to Manage e-Mail Records)

  • 김용;김늘봄;이태영
    • 정보관리학회지
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    • 제25권4호
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    • pp.87-113
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    • 2008
  • 본 연구는 호주 이메일메타데이터표준 및 한국기록관리메타데이터 표준에 기반하여 국제표준을 준용하는 이메일기록에 대한 표준화된 메타데이터요소를 개발하는데 있다. 이러한 목표를 달성하기 위하여 본 연구에서는 기록관리 및 기록물 메타데이터와 관련된 국제표준인 ISO 15489와 ISO 23081을 분석 및 조사하였다. 두번째로, 국내 공공기관에서 생산된 이메일기록에 대한 특징을 추출하기 위하여 사례연구를 수행하였다. 또한, 호주의 이메일 메타데이터표준에 대한 상대평가 연구를 수행하였다. 위의 과정을 통하여 추출된 결과와 함께, 본 연구에서는 이메일기록에 대한 관리를 위한 필수 및 선택적 메타데이터요소를 제안하였다. 또한 제안된 이메일 메타데이터요소에 적용 가능한 XML DTD를 개발하였다. 한편, 제안된 이메일 메타데이터요소는 한국의 지방자치단체의 행정정보시스템에서 생산된 실제 이메일기록에 적용하였다.

국내 OEM/ODM 화장품 제조기업의 생산계획 및 효율화를 위한 분산형 MRP시스템 개발 (Development of Distributed MRP System for Production Planning and Operation in Korean OEM/ODM Cosmetics Manufacturing Company)

  • 장동민;신문수
    • 산업경영시스템학회지
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    • 제43권4호
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    • pp.133-141
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    • 2020
  • Up to date cosmetic OEM/ODM (original equipment manufacturing/original development manufacturing) industry receives attention as a future growth engine due to steady growth. However, because of limited research and development capability, many companies have employed commercial management platforms specialized for large-sized companies; thus, overall system effectiveness and efficiency is low. Especially, MRP (material requirement planning) system introduced originally in 1970s is employed to calculate the requirement of the parts. However, dynamic nature of production lead time usually results in incorrect requirements. In addition, its algorithm does not consider the capability of the production resources. Also, because the commercial MRP system calculates all subcomponent for fixed period, the more goods have subcomponent, the slower calculation is. Therefore, conventional MRP system cannot respond complicated situation in time. In this study, we will suggest a new method that can respond to complicated situations resulting from short lead time and urgent production order in Korean cosmetic market. In particular, a distributed MRP system is proposed, that consists of multi-functional and operational modules, based on the characteristic of the BOM (bill of material). The distributed MRP system divides components (i.e. products and parts) into several fields and decrease the problem size; thus, we can respond to dynamically changed data any time. Through this solution, we can order components quickly, adjust schedules and planned quantity, and manage stocks reasonably. In addition, a prototype of the distributed MRP system is presented in this paper, in which ERP (enterprise resource planning) sever data is associated with an excel spreadsheet via MSsql. System user interface is implemented by a VBA (visual basic for applications) tool. According to a case study, response rate for delivery and planning achievement rate were enhanced about 20%, and inventory turnover was also decreased. Consequently, the proposed system improves overall profit.

잦은 감기나 만성 비염을 동반한 마른 체형 소아에 대한 보폐성장증보탕 (補肺成長增補湯)의 체중개선 효과 - 의무기록 분석 - (The Weight Gain Effects of the Bofesungjangjeungbo-tang (Bǔfèichéngzhǎngzēngbǔ-tang) on Under-weight Korean Preschool Children with Frequent Common Cold or Chronic Rhinitis - Analysis of Medical Records -)

  • 김기준;이준석;윤지현;류봉하;백희영
    • 대한한방소아과학회지
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    • 제26권4호
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    • pp.44-50
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    • 2012
  • Objectives: We investigated weight gain effects of the Bofesungjangjeungbo-tang ($B\check{u}f\grave{e}ich\acute{e}ngzh\check{a}ngz\bar{e}ngb\check{u}$-tang) on thin Korean preschool children with frequent common cold or chronic rhinitis Methods Bofesungjangjeungbo-tang was empirically composed of 14 herbs for treating indigestion, and common cold or chronic rhinitis. We analyzed 60 preschool children who have visited Kim Kijoon Oriental Clinic BOM from Jan. 1, 2006 to Aug. 31, 2011 with three criteria: 1) 2~5yrs of age and BMI<50th percentile 2) children those who had taken the Bofesungjangjeungbo-tang for 1~3 month (s), 3) Availability of records on before/after treatment within 3 months. Weight gain of the children after treatment was evaluated by the changes of BMI percentile compared to pre-treatment. Results Bofesungjangjeungbo-tang significantly improved BMI percentile in all groups (1 month: $21.33{\pm}10.04$ vs $26.43{\pm}13.16$, p<0.001; 2 months: $21.90{\pm}10.28$ vs $29.03{\pm}15.06$, p<0.001; 3 months: $19.06{\pm}10.04$ vs $35.71{\pm}17.40$, p=0.001). Gender, age and pre-treatment BMI had no significant effects on the effectiveness of Bofesungjangjeungbo-tang. Conclusions: Bofesungjangjeungbo-tang positively affected weight gain in 2~5yrs Korean preschool children with BMI below 50 percentile within 1~3 month (s) of treatment. However, additional studies on functional dyspepsia or the change of energy intake are needed to understand the factors related to weight gains.

자동차 부품의 리사이클성을 고려한 제품구조.구성기 구현에 관한 연구 (Development of Product Structure and Configuration Management System for Recycling of Automobile Parts)

  • 추헌진;이건상;이강수
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2001년도 춘계학술대회논문집C
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    • pp.229-234
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    • 2001
  • In order to care about preserving the environment for future generations, each country has been required to make the laws of the environment. So car manufacturers must design, manufacture and sell cars and trucks that meet customer's need and must do this while meeting the needs of the environment. In this research we have developed product structure and configuration management system that treats electronic catalogs for recycling of automobile parts. This research focused on two system. One is XML-based electronic catalog that give the information about recycling of automobile parts. The other is product structure and configuration management system based on electronic catalog.

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Active Targeting of Tumor Cells using Light Emitting Bacteria

  • Moon Sung-Min;Min Jung-Joon;Hong, Yeong-Jin;Kim, Hyun-Ju;Le, Uyenchi N.;Rhee Joon-Haeng;Song, Ho-Chun;Heo Young-Jun;Bom, Hee-Seung;Choy Hyon-E.
    • 대한핵의학회:학술대회논문집
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    • 대한핵의학회 2004년도 추계학술대회 및 총회
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    • pp.392.2-392.2
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    • 2004
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갑상선 유두암 환자의 갑상선엽절제술 후유증에 대한 증례 보고 3례 (Three Cases of Sequela following Thyroid Lobectomy for Thyroid Cancer)

  • 조수연;안뜰에봄;김동철
    • 대한한방부인과학회지
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    • 제31권3호
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    • pp.141-151
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    • 2018
  • Objectives: This paper aims to report the effects of Korean medical treatment on three patients who underwent thyroid lobectomy for thyroid cancer. Methods: Three patients were hospitalized at hospital to treat pain and general weakness after thyroid lobectomy. The patients took Korean medical treatment including herbal medicine and acupuncture. Numerical Rating Scale (NRS) of symptoms was measured every morning. Results: After the treatment, the pain and general weakness following surgery decreased, and general conditions improved. Conclusions: This cases show that Korean medical treatment is effective for a patients who underwent thyroid lobectomy for thyroid cancer.

익위승양탕가감방을 통해 월경통이 호전된 자궁선근증 환자 1례 (Yikwiseungyang-tang-gagam-bang Improved Dysmenorrhea in Patient with Adenomyosis: A Case Report)

  • 안뜰에봄;정현정
    • 대한암한의학회지
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    • 제22권2호
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    • pp.33-38
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    • 2017
  • Objective: The purpose of this study is to report the effect of Yikwiseungyang-tang-gagam-bang on Dysmenorrhea with Adenomyosis. Methods: The patient was a secondary dysmenorrhea with adenomyosis accompanied by menorrhagia. She took 2 packs of herbal medicine, Yikwiseungyang-tang-gagam-bang, a day, and received acupuncture treatment at point of CV 4, EX-CA1, CV 12, LI 4, LR 3, SP 6, ST 36 three times a week for two menstrual cycle. Results: After treatment, menstrual pain was reduced from VAS 10 to 1. In addition, menstrual fluid volume also were reduced from VAS 10 to 6. The quality of life is also improved, so sleep and digestion problems improved. Conclusion: This study suggests that Oriental medicine treatment including Yikwiseungyang-tang-gagam-bang may have the efficacy for the treatment of Dysmenorrhea with Adenomyosis.