• Biomedical Science Letters
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• v.18 no.1
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• pp.29-34
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• 2012

Streptomycetes are gram-positive filamentous bacteria that are well-known for producing a vast array of bioactive compounds, including more than 70 % of commercially important antibiotics. For the research about Streptomyces sp., the protoplast and electroporation transformation method have been the general techniques for the construction of transformants. However, these techniques have low efficiency and are time-consuming. Another option is intergenic conjugation, which is used for DNA transfer using methylation-deficient E. coli as a DNA donor to avoid the methylated-DNA-dependent restriction systems of actinomycetes. This conjugation method has been widely improved and applied to many other actinomycetes. In this research, an effective transformation procedure for the construction of expression vector by using gateway system was established to avoid limit of restriction enzyme site for cloning of target gene based on transconjugation by Escherichia coli ET12567/pUZ8002 with a pSET152 integration vector.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2002.10a
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• pp.393-398
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• 2002

This study proposes a method to reduce error scanning data of laser scanner. The method co of 3 stages. First, there is an error indu difference of the distance between the prob the object. It is possible to reduce the e planning a scanning strategy: object settin path. Second, the scan data loss of the tooli affects calculating the tooling ball con z-direction compensation is given to calculat accurate registration points. Third, three p used to determine a transformation matrix on frame. As merging, the maximum error usually on the third tooling ball in the conven method, which select a point among three po randomly. We find the centroid of 3 points apply it to determine a new transformation mat

• Molecules and Cells
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• v.21 no.2
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• pp.167-173
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• 2006

The literature provides strong precedent for both pro-tumorigenic and tumor suppressor roles for the c-Jun N-terminal kinases (JNKs) in the setting of oncogenesis. Clearly, JNKs are activated by numerous oncogenes and growth factors and the literature documents a role for these MAP kinases in cell proliferation and transformation. By contrast, JNKs mediate signals from diverse stimuli that result in cell death or differentiation and a role for JNKs as tumor suppressors has emerged. This enigmatic nature of the JNKs in the setting of oncogenesis is considered herein. Further illumination of the complex and context-dependent functions of the JNKs in cancer cells is of obvious importance for the rational use of small molecule JNK inhibitors as therapeutics.

• Journal of Microbiology and Biotechnology
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• v.21 no.7
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• pp.675-678
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• 2011

DNA methylation in Streptomyces griseus IFO 13350 was analyzed by high-performance liquid chromatographic analysis and bisulfite-based analysis to reveal two methylation sites, 5'-$GC^{5m}$ CGGC-3' and 5'-$GAG^{5m}$ CTC-3'. The methylation was reconstituted in Escherichia coli by simultaneous expression of S. griseus SGR4675 and S. achromogenes M.SacI. The E. coli cells produced plasmids that mimicked the methylation profile of S. griseus DNA, which was readily introduced into S. griseus. The results of this study raise the possibility of a promising approach to establish efficient transformation in several streptomycetes.

• ALGAE
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• v.33 no.4
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• pp.351-358
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• 2018

The ability to transform the chloroplast of Cyanidioschyzon merolae was limited by lack of confirmed and reliable promoter sequences (among other reasons), capable of delivering stable or modulated DNA transcription followed by protein synthesis. Our research has confirmed the applicability of three selected chloroplast promoters in C. merolae chloroplast overexpression of the exogenous protein (i.e., chloramphenicol acetyltransferase) and genetic transformation. These results might facilitate further research on genetically modified strains of C. merolae to envisage yet unknown aspect of cellular and plastic physiology as well as C. merolae potential applications as bio-factories or sources of useful chemicals.

• Communications of the Korean Mathematical Society
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• v.37 no.1
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• pp.213-228
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• 2022

In the present paper, we aim to study Yamabe soliton and Riemann soliton on Lorentzian para-Sasakian manifold. First, we proved, if the scalar curvature of an 𝜂-Einstein Lorentzian para-Sasakian manifold M is constant, then either 𝜏 = n(n-1) or, 𝜏 = n-1. Also we constructed an example to justify this. Next, it is proved that, if a three dimensional Lorentzian para-Sasakian manifold admits a Yamabe soliton for V is an infinitesimal contact transformation and tr 𝜑 is constant, then the soliton is expanding. Also we proved that, suppose a 3-dimensional Lorentzian para-Sasakian manifold admits a Yamabe soliton, if tr 𝜑 is constant and scalar curvature 𝜏 is harmonic (i.e., ∆𝜏 = 0), then the soliton constant λ is always greater than zero with either 𝜏 = 2, or 𝜏 = 6, or λ = 6. Finally, we proved that, if an 𝜂-Einstein Lorentzian para-Sasakian manifold M represents a Riemann soliton for the potential vector field V has constant divergence then either, M is of constant curvature 1 or, V is a strict infinitesimal contact transformation.

• Journal of Microbiology
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• v.43 no.4
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• pp.337-344
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• 2005

Although pneumococcus is one of the most frequently encountered opportunistic pathogen in the world, the mechanisms responsible for its infectiveness have not yet been fully understood. In this paper, we have attempted to characterize the effects of pneumococcal transformation on the pathogenesis of the organism. We constructed three transformation-deficient pneumococcal strains, which were designated as Nos. 1d, 2d, and 22d. The construction of these altered strains was achieved via the insertion of the inactivated gene, comE, to strains 1, 2 and 22. We then conducted a comparison between the virulence of the transformation-deficient strains and that of the wild-type strains, via an evaluation of the ability of each strain to adhere to endothelial cells, and also assessed psaA mRNA expression, and the survival of hosts after bacterial challenge. Compared to what was observed with the wild-type strains, our results indicated that the ability of all of the transformation-deficient strains to adhere to the ECV304 cells had been significantly reduced (p < 0.05), the expression of psaA mRNA was reduced significantly (p < 0.05) in strains 2d and 22d, and the median survival time of mice infected with strains Id and 2d was increased significantly after intraperitoneal bacterial challenge (p < 0.05). The results of our study also clearly indicated that transformation exerts significant effects on the virulence characteristics of S. pneumoniae, although the degree to which this effect is noted appears to depend primarily on the genetic background of the bacteria.

• Journal of The Korean Astronomical Society
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• v.53 no.5
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• pp.99-102
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• 2020

I show that when the observables (π_E, t_E, θ_E, π_s, µ_s) are well measured up to a discrete degeneracy in the microlensing parallax vector π_E, the relative likelihood of the different solutions can be written in closed form P_i = KH_iB_i, where H_i is the number of stars (potential lenses) having the mass and kinematics of the inferred parameters of solution i and B_i is an additional factor that is formally derived from the Jacobian of the transformation from Galactic to microlensing parameters. Here t_E is the Einstein timescale, θ_E is the angular Einstein radius, and (π_s, µ_s) are the (parallax, proper motion) of the microlensed source. The Jacobian term B_i constitutes an explicit evaluation of the "Rich Argument", i.e., that there is an extra geometric factor disfavoring large-parallax solutions in addition to the reduced frequency of lenses given by H_i. I also discuss how this analytic expression degrades in the presence of finite errors in the measured observables.

• Journal of the Korean Society for Heat Treatment
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• v.11 no.2
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• pp.111-120
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• 1998

The effects of chemical composition and thermal cycling on the martensitic transformation characteristics in Cu-rich, equiatomic and Zr-rich CuZr binary alloys have been studied by calorimetry. Only martensite could be indentified in equiatomic $Cu_{49.9}Zr_{50.1}$ alloy, while $Cu_{10}Zr_7$ and $CuZr_2$ intermetallic compounds as well as martensite were formed by rapid cooling from the melts in Cu-rich $Cu_{52.2}Zr_{47.5}$ alloy and Zr-rich $Cu_{48.4}Zr_{51.6}$ alloy, respectively. The $M_s$ temperature of $Cu_{49.9}Zr_{50.1}$ was $156^{\circ}C$ but those of $Cu_{52.5}Zr_{47.5}$ and $Cu_{48.4}Zr_{51.6}$ alloys, being $109^{\circ}C$ and $138^{\circ}C$, were lower than that of equiatomic $Cu_{49.9}Zr_{50.1}$ alloy. In all the alloys, the $M_s$ temperature has fallen but the $A_s$ temperature has risen, resulting in widening of the transformation hysteresis with thermal cycling. The anomalous characteristics in the transformation temperature are due to the presence of the intermetallic compounds i.e. $Cu_{10}Zr_7$ and $CuZr_2$ formed by an eutectoid reaction during thermal cycling in the temperature range between $-100^{\circ}C$ < $T_c$ < $400^{\circ}C$.

• Journal of Korea Multimedia Society
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• v.7 no.9
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• pp.1195-1207
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• 2004

Schema matching is prerequisite to an automated transformation of XML documents. Because previous works about schema matching compute all semantically-possible matchings, they produce many-to-many matching relationships. Such imprecise matchings are inappropriate for an automated transformation of XML documents. This paper presents an efficient schema matching algorithm that computes precise one-to-one matchings between two schemas. The proposed algorithm consists of two steps: preliminary matching relationships between leaf nodes in the two schemas are computed and one-to-one matchings are finally extracted based on a proposed path similarity. Specifically, for a sophisticated schema matching, the proposed algorithm is based on a domain ontology as well as a lexical database that includes abbreviations and synonyms. Experimental results with real schemas from an e-commerce field show that the proposed method is superior to previous works, resulting in an accuracy of 97% in average.