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http://dx.doi.org/10.4014/jmb.1102.02038

Improvement of Transformation Efficiency by Strategic Circumvention of Restriction Barriers in Streptomyces griseus  

Suzuki, Hirokazu (Chemical Biology Department, Advanced Science Institute, RIKEN)
Takahashi, Shunji (Chemical Biology Department, Advanced Science Institute, RIKEN)
Osada, Hiroyuki (Chemical Biology Department, Advanced Science Institute, RIKEN)
Yoshida, Ken-Ichi (Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.7, 2011 , pp. 675-678 More about this Journal
Abstract
DNA methylation in Streptomyces griseus IFO 13350 was analyzed by high-performance liquid chromatographic analysis and bisulfite-based analysis to reveal two methylation sites, 5'-$GC^{5m}$ CGGC-3' and 5'-$GAG^{5m}$ CTC-3'. The methylation was reconstituted in Escherichia coli by simultaneous expression of S. griseus SGR4675 and S. achromogenes M.SacI. The E. coli cells produced plasmids that mimicked the methylation profile of S. griseus DNA, which was readily introduced into S. griseus. The results of this study raise the possibility of a promising approach to establish efficient transformation in several streptomycetes.
Keywords
DNA methylation; DNA restriction; host-mimicking DNA; Streptomyces griseus;
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