• 제목/요약/키워드: dye experiment

검색결과 179건 처리시간 0.023초

사람 및 생쥐 백혈병 억제인자가 소 체외성숙, 체외수정란의 발육에 미치는 효과 (Effects of Human or Mouse Leukemia Inhibitory Factors on the Development of Bovine IVM/IVF Embryos)

  • 양부근;김정익
    • 한국가축번식학회지
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    • 제18권2호
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    • pp.105-111
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    • 1994
  • The effects of human or mouse leukemia inhibitory factor(hLIF or mLIF) were examined as a means of increasing the development of in vitro matured(IVM) and in vitro fertilized (IVF) oocytes into morulae or blastocysts. Cell numbers of blastocysts were also counted using Hochest dye staining. Two-to 8-cell embryos derived from bovine IVM/IVF oocytes were cultured 5 to 6 days in CRI aa with or without mLIF or hLIF. All culture media were contained 3mg/ml bovine serum albumin. In experiment 1, the proportion of embryos developed to morulae and blastocysts in CRI aa containing 5,000U/ml mLIF(37.8%) was slightly higher than those of CRIaa containing 1,000U/ml mLIF(34.6%) and 0 U/ml mLIF(27.4%; P>0.05). In experiment 2, 0, 1,000 and 5,000U/ml of hLIF added to CR1aa media yielded 27.6%, 43.0% and 35.5% morulae and blastocysts, respectively(p>0.05). These were no significant increases in cell number among treatments(p>0.05). These results were indicating that mLIF or hLF can increase the proportion of embryos that develop into morulae and blastocysts without and increase in the cell number.

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A Study on the General Characteristics and Instrumental Analysis of Natural Omija Extract

  • Sung, Ki-Chun;Kim, Ki-Jun;Kim, Yong-Ryul;Nam, Sang-Sung
    • 한국응용과학기술학회지
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    • 제30권2호
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    • pp.225-232
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    • 2013
  • Omija component was known to possess natural odor, taste, color, and various general characteristics. Omija extraction was extracted using ethanol as a solvent. Omija extract showed a red-purple color of some viscous liquid state. Some conclusions from natural Omija extract were obtained as follow. The result of antimicrobial experiment to add Omija extract, the number of microbial population showed negative reaction from 3 days after it cultivated. This phenomenon could confirm that Omija component affected to antimicrobial effect. The result of dyeing experiment to add Omija extract, fiber dyeing effect showed with some ivory color after dyed to cotton and silk. Also, this phenomenon could confirm that Omija component affected to natural dyeing effect from observated dye state with biological microscope(BM). The result of instrumental analysis, inorganic components of K(109.60ppm), Na(3.500ppm), Ca(1.205ppm), Mg(0.900ppm), Li(0.350ppm), Si(0.380ppm), Cu(0.250ppm), Fe(0.125ppm), Zn(0.090ppm), etcs from Omija were ascertained with ICP/OES, and organic components of benzene(10.808), borny lacetate(11.289), phenol(14.183), ${\beta}$-terpinene(15.840), ${\alpha}$-terpinolene(17.616) etcs from Omija were ascertained with GC/MSD.

추적자 실험 및 3차원 수치모의를 이용한 저수지 수처리 장치의 영향반경 평가 (Assessing the Influence Radius of a Water Treatment System Installed in a Reservoir Using Tracer Experiment and 3D Numerical Simulation)

  • 박형석;이은주;지현서;최선화;정세웅
    • 한국수처리학회지
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    • 제26권6호
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    • pp.3-12
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    • 2018
  • The objective of this study was to evaluate the radius of influence of effluent of water treatment system developed for the purpose of improvement of reservoir water quality using fluorescent dye (Rhodamine-WT) tracer experiment and 3-D numerical model. The tracer experiment was carried out in a medium-sized agricultural reservoir with a storage capacity of $227,000m^3$ and an average depth of 1.6 m. A guideline with a total length of 160 m was installed at intervals of 10 m in the horizontal direction from the discharge part, and a Rhodamine measurement sensor (YSI 6130, measurement range $0-200{\mu}g/L$) was used to measure concentration changes in time, distance, and depth. Experimental design was established in advance through Jet theory and the diffusion process was simulated using ELCOM, a three dimensional hydraulic dynamics model. As a result of the study, the direct effect radius of the jet emitted from the applied water treatment system was about 50-70 m, and the radius of physical effect by the advection diffusion was judged to be 100-120 m. The numerical simulations of effluent advection-diffusion of the water treatment system using ELCOM showed very similar results to those of the impact radius analysis using the tracer experiment and jet flow empirical equations. The results provide valuable information on the spatial extent of the water quality improvement devices installed in the reservoir and the facility layout design.

Evaluation of Lac Cultivation in Two South-Western Districts of Bangladesh

  • Bahar, Habibullah;Islam, Tarikul;Islam, Monirul;Mannan, Abdul
    • International Journal of Industrial Entomology and Biomaterials
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    • 제15권1호
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    • pp.79-82
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    • 2007
  • Lac is the dermal secretion of lac insect, Kerria lacca Kerr., which is used to make expensive natural dye, burnish, coating materials, in cosmetics and jewelry industry. Though it is very perspective industry and available of host plants over the country especially in southern districts of Bangladesh, lac cultivation is confined within some northern districts of Bangladesh. Experiment was conducted to find out the possibility of lac cultivation in two southwestern districts of Bangladesh: Khulna and Satkhira compared to Chapainowabgonj, the key lac producing district in Bangladesh, during February-October, 2006. The bark thickness, bark weight of ber plant, ratio of harvested and inoculated lac sticks, harvested raw lac, and harvested processed lac were measured and compared. Statistically, similar performance of lac cultivation was found regarding all parameters in three districts. These results recommended that lac cultivation is possible at the south-western coastal part of Bangladesh.

아연 매염제를 이용한 뽕잎, 커피 천연염재의 염색특성 (The Characteristic of Natural Dyeing with Mulberry Leaf and Coffee Powder using Zinc Mordant)

  • 이종관;고재훈
    • 한국염색가공학회지
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    • 제28권2호
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    • pp.118-123
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    • 2016
  • The use of natural organic dyes obtained from renewable resources such as plants and trees has the potential for not only preserving petrochemicals but also all-endangered environment for coming next generations. In spite of inferior fastness, natural dyes can be employed in the colouration of natural as well as synthetic fibers. Recently, the potentiality of using natural dyes in colouration with additional UV-protection and antimicrobial properties has been reported. This study is aimed to investigate the effect of Zinc compounds compared to another metallic compounds as mordants on the dyeing properties of natural dye extracted from mulberry leaves and coffee. In this study, the results showed Zinc compounds was expose that is more similar than other mordants in washing fastness through a repeated experiment. Also, Zinc is the environmental impact is less material compared with other mordants, essential edibility minerals ingredient that is necessary in human body.

Effects of Reynolds Number and Shape of Manifold on Flow Rate in Separator for Polymer Electrolyte Fuel Cell (ICCAS 2004)

  • Huang, Chaii;Ozawa, Yoshikuni;Ennoji, Hisayuki;Iijima, Toshio
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 2004년도 ICCAS
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    • pp.68-71
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    • 2004
  • Recently, a great deal of research and development of a fuel cell have been carried out to solve problems on the drain of fossil fuel, air pollution and global warning. In order to improve the efficiency of a fuel cell, it is necessary to clarify the flow in separator. In this study, distributions of velocity flow rate and pressure, and streamlines are examined in detail from numerical analysis with CFD code. In the experiment the distribution of flow rate is measured and flow in the each grooves of the separator is visualized by dye method changing Reynolds number. Furthermore, effects of size of the inlet and outlet manifolds and shape of ribs near the inlet outlet on the distributions of flow and pressure are examined.

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온도 민감 형광을 이용한 마이크로 스케일 표면온도 측정 (Surface Temperature Measurements in Microscale with Temperature Sensitive Fluorescence)

  • 정운섭;김성욱;김호영;유정열
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2004년도 추계학술대회
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    • pp.1225-1230
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    • 2004
  • The effects of substrate material on the local heating performance of microheaters are studied by both numerical analysis and experiment. Transient conduction analysis shows that the substrate material with low thermal conductivity is critical to the local heating and fast response. A measurement technique for surface temperature field in microscale is newly proposed, which uses temperature sensitive fluorescent dye coated on the surface. The measured surface temperature fields on microheater arrays fabricated on different substrates are presented.

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색지 폐지의 광학적 특성에 미치는 환원 표백의 영향 (Effect of Reductive Bleaching on Optical Properties of Color Ledger)

  • 안병준;백기현
    • 펄프종이기술
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    • 제33권2호
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    • pp.68-73
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    • 2001
  • This experiment was executed to clarify the optimum condition of reductive bleaching for 100% color papers. The reductive bleaching of the color paper was desirable to be done under such conditions as high temperature over $60^{\circ}C$ and high consistency within possibly short time. The FAS bleaching like the sodium hydrosulfite required oxygen-free condition. Especially, as the mixture between pulps and chemicals was favored in case of the high consistency, it was actively recommended. Therefore, it is desirable to control the dosage with the type of the dyes, the nature, the dye content, the paper compositions, and the targeting initial and final brightness.

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천연염료를 이용한 한지염색에 관한 연구(II) -양파껍질을 중심으로- (Studies on the Dyeing of Hanji by Natural Dye-stuffs(II) -With a focus on the Onion-peelings-)

  • 전철
    • 펄프종이기술
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    • 제35권1호
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    • pp.48-53
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    • 2003
  • The objective of this study was to find in what color Korean handmade paper(Hanji) is dyed when it is dyed with a pigment extracted from waste onion peelings using different kinds of mordant, and how the paper is discolored and variety of strength under the condition of accelerated aging test. The results of this experiment are as follows. 1. Korean handmade paper was dyed in different colors according to the kinds of mordant. Mainly it was dyed in orange-brown, and sometimes in gold or khaki. 2. Korean handmade paper dyed was not discolored much, which suggests that the pigment is strong under the condition of aging. 3. As for durability, Korean handmade paper dyed in gold was strongest. Korean handmade paper, to which aluminum sulfate, iron sulfate or sodium dichromate was applied as mordant, had poor durability. Thus, these were not suitable as mordant.

Multi-Domain과 행렬 보간법을 이용한 강 하구와 연안의 2차원 수치해석 (Numerical Simulation of 2-D Estuaries and Coast by Multi-Domain and the Interpolating Matrix Method)

  • 채현식
    • 한국전산유체공학회지
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    • 제2권1호
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    • pp.21-28
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    • 1997
  • This paper presents a two-dimensional horizontal implicit model to general circulation in estuaries and coastal seas. The model is developed in non-orthogonal curvilinear coordinates system, using the Interpolating Matrix Method (IMM), in combination with a technique of multi-domain. In the propose model, the Saint-Venant equations are solved by a splitting-up technique, in the successive steps; convection, diffusion and wave propagation. The ability of the proposed model to deal with full scale nature is illustrated by the interpretation of a dye-tracing experiment in the Gironde estuary.

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