• Title/Summary/Keyword: dual culture

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Evaluation of Bio-Control Efficacy of Trichoderma Strains against Alternaria alternata Causing Leaf Blight of Ashwagandha [Withania somnifera (L.) Dunal]

  • Rahman, Md. Ahsanur;Rahman, Md. Arifur;Moni, Zakiah Rahman;Rahman, Mohammad Anisur
    • Journal of Forest and Environmental Science
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    • v.36 no.3
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    • pp.207-218
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    • 2020
  • Ashwagandha is an important ancient medicinal crops, being affected with many diseases, among which leaf blight caused by Alternaria alternata has become the constraint resulting in huge yield losses. Continuous usage of chemical methods leads to environment, soil and water pollution. Whereas biological control of diseases is long lasting, inexpensive, eco-friendly and harmless to target organisms. In this context, it is aimed to evaluate five Trichoderma strains viz. Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433 and T. harzianum IMI-392434 as bio-control efficacy against A. alternata and growth promoting effect in Ashwagandha. All the Trichoderma strains had varied antagonistic effects against the pathogen. In dual culture technique, the strain T. harzianum IMI-392433 showed maximum percentage inhibition of mycelial growth (54.89%) followed by T. harzianum IMI-392432 (53.83%), T. harzianum IMI-392434 (48.94%) and T. virens IMI-392430, (43.62%) against the pathogen, while the least inhibition percentage was observed with the T. pseudokoningii IMI-392431 (36.60%). The culture filtrate of the Trichoderma strain, T. harzianum IMI-392433 recorded highest inhibition on the mycelial growth (39.05%) and spore germination (80.77%) of pathogen and the lowest was recorded in T. pseudokoningii IMI-392431 (20.45 and 50%). Moreover, seeds treated with spore suspension of the strain T. harzianum IMI-392433 reduced the percentages of disease severity index significantly. The strain T. harzianum IMI-392433 also significantly increased seed germination %, seedling vigor and growth of Ashwagandha. The correlation matrix showed that root yield per plant of Ashwagandha had significant and positive correlation with plant height (r=0.726⁎⁎), number of leaf (r=0.514⁎⁎), number of primary branch (r=0.820⁎⁎), number of secondary branch (r=0.829⁎⁎), fresh plant weight (r=0.887⁎⁎), plant dry weight (r=0.613⁎⁎), root length (r=0.824⁎⁎), root diameter (r=0.786⁎⁎), root dry weight (r=0.739⁎⁎) and fresh root weight (r=0.731⁎⁎). The significant and negative correlation (r=-0.336⁎⁎) was observed with the root yield and percentages of disease severity index. The study recognized that the T. harzianum IMI-392433 strain performed well in inhibiting the mycelial growth and reduced the percentages of disease severity index of pathogen as well as increased the plant growth in Ashwagandha.

IAA-Producing Penicillium sp. NICS01 Triggers Plant Growth and Suppresses Fusarium sp.-Induced Oxidative Stress in Sesame (Sesamum indicum L.)

  • Radhakrishnan, Ramalingam;Shim, Kang-Bo;Lee, Byeong-Won;Hwang, Chung-Dong;Pae, Suk-Bok;Park, Chang-Hwan;Kim, Sung-Up;Lee, Choon-Ki;Baek, In-Youl
    • Journal of Microbiology and Biotechnology
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    • v.23 no.6
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    • pp.856-863
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    • 2013
  • Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growth-promoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.

Archeological Quest on the Origin and Formation of the Stone Needle in the Korean Peninsula (폄석(砭石)의 한반도 기원과 형성에 관한 연구)

  • Yim, Yong-Soo;Sohn, In-Chul;Kang, Yeon-Seok;Kim, Seong-Chul;Kim, Jae-Hyo
    • Korean Journal of Oriental Medicine
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    • v.15 no.2
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    • pp.51-61
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    • 2009
  • Objective: Much has been known that the systematical theory of acupuncture was developed from stone needle as therapeutic tools for orthopedic diseases in ancient times. Stone needle is very old therapeutic method like moxibustion and has been recognized that it was developed since the Stone Age in China so far. In the present study, it was examined for the origination and formation of stone needle based on stone relics of the Stone Age in Korean Peninsular, the medical and geographical literatures. Materials and methods: The facts of stone needle was examined and arranged on the ancient medical or geographical literatures such as The Yellow Emperor's Canon Internal Medicine, Shanhaijing as an ancient geographical book, etc. The clan societies and family related to an origination of stone needle was chased together with their cultural characteristics and origination. The stone relics which have been digged out of historic sites in the North-East Asia were examined for a relevance to stone needle. Results: In The Yellow Emperor's Canon Internal Medicine, it was referred to the stone needle that originated from a fishery zone related to the east coast district in North-East Asia. Through the examination of Shan Hai Jing as an ancient geographical book and its historical reviews, a Go-yi clan society who keep Go's family tree dealt well with the stone needle and jewels including jade in the North-East Asia before the publication periods of The Yellow Emperor's Canon Internal Medicine, and is comprised in the culture of Dong-yi clan society but not the Chinese culture. The obsidian stones, which have been digged out of historical sites in the North-East Asia since the Stone Age, are originated from volcanic areas combined with seashore that seems to be Baekdu mountain district in Korea and Kyushu district in Japan. Furthermore, obsidian stone tools which were found out at Laodung peninsula and the Korean peninsula are archeologically similar to the stone needle with regards to the shape, size and dual-use. In addition, specific obsidian stone tools have been used in orthopedic surgery as well-crafted obsidian blades have a cutting edge up to five times sharper than high-quality steel surgical scalpels. Conclusion: The origin of obsidian stone needle is well corresponded to the explanation about that of the stone needle. It is suggested that the stone needle which influenced in completion of acupuncture and Meridian theory in China seems to be an obsidian stone, and distribution of obsidian stone needle has been closely connected to Dong-yi clan society which are lived in the North-East Asia including Baekdu-mountain district.

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An Ethnographic Research Study on Childbearing Process of Mother with Children in Korea (자녀를 둔 어머니의 출산과정 경험)

  • Kim, Young-Hee
    • Women's Health Nursing
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    • v.7 no.3
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    • pp.271-283
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    • 2001
  • The childbearing process is a sociocultural phenomenon of a woman who gives birth to a child as well as a biological phenomenon. The purpose of this ethnographic research study was to explore the experience of childbearing process of mothers with children from pregnancy to the 3 months postpartum in Korea and to understand deeply the perspectives of childbearing women reflected on Korean sociocultural values. A convenient sample of 10 childbearing women were observed from January to October 2000 through field work in Seoul, Korea. Data analysis was accomplished under ongoing process. The results of this study were as follows : The mothers with children experienced self-reflection, family relation, and physical adaptation during pregnancy. In self-reflection, all mothers experienced universality and diversity in their self-discovering process. The universal experiences were maturation, life with family and priority on maternal value between being a mother and a woman. The diverse experiences were taking a dual role of working mother, emotional drift of a resigned mother, and disheartened life of a mother who has two daughters. In family relation, the foundation of the new marital relationship were attained during childbearing process and sexual life were changed for the benefit of a healthy mother and a healthy baby. All mothers established friendly relations with their mothers, but established friendly or conflicting or constraining relations with their mother-in-laws due to husband based family culture. In physical adaptation, the informants endured well the physical discomfort and recognized general appearance change. Also maternal-fetal interaction occurred and mothers realistically felt motherhood and accepted themselves as mother-to-be. The mothers prepared for the best delivery, look for a safe childbirth center, newborn goods, endorsed family coping during hospitalization and responded labor pain to make it more endurable, less painful, fast passed owing to labor recognition of the natural process to be a mother. After childbirth, they felt emancipation, satisfaction, accomplishment, more easiness, actually feeling as mother-to-be, emptiness, and showed response to the sex of newborn. Their Sanhujori practice was different according to the Sanhujori environment including provider, place, time in postpartum and reflected on Sanhubyung. The mothers felt actually mother-to-be and happiness during lactation regardless of feeding pattern. These mothers had a different maternal image about rearing subjecthood through their child-rearing experience. But all mothers felt need for family support and social support. The universal rearing response were actual feeling of mother-to-be, a strenuous experience, a pride on child-rearing, confusion, reflecting marital relationship, and wondering rivalry among children. In conclusion, mother of all with children went through self-discovery, self-reflection and made connections with the family as a mother and as a woman simultaneously during the childbearing process. Therefore it is suggested when harmony and balance between a mother and a woman is accomplished, the woman will lead a healthy and high quality of life. Also, this study sought to confirm the sociocultural factors affecting the childbearing process from the perspectives of the women with children. Therefore health care providers must understand deeply the childbearing women with children based on this finding of and try a integrative approach with new ideology of maternity with biocultural perspectives in a clinical setting.

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The Role of Cyber in Kim Jong Un's Byungjin Line: North Korea's Political Culture, Hackers, and Maritime Tactics (김정은의 병진노선에서 사이버의 역할: 북한의 정치문화, 해커, 해양전술)

  • Young, Benjamin R.
    • Maritime Security
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    • v.3 no.1
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    • pp.45-72
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    • 2021
  • North Korea's cyber capabilities represent a relatively new threat to global financial institutions and foreign governments, particularly the U.S and South Korean governments. Based primarily on publicly available sources, such as journalistic accounts and scholarly publications, this qualitative paper analyzes the ways in which North Korean leader Kim Jong Un has bolstered his country's asymmetric power and advanced his line of byungjin (dual development in the economy and military). Particularly by merging the cyber and maritime domains, North Korean operatives generate more revenue for the regime and helps keep the heavily sanctioned leadership in power. Despite the increased international attention to North Korean hackers, few analysts have examined the important role of cyber in the DPRK's internal political culture, specifically in advancing Kim Jong Un's byungjin line. Cyber fits into the DPRK's longstanding tradition of irregular warfare and guerilla-based armed struggle. Cyber also further advances Kim's personal reputation in the DPRK as an economic innovator and military strategist. This paper pays particular attention to the role of the DPRK's cyber operations in both ideological and maritime contexts. Recently, North Korean hackers have targeted South Korean shipbuilding industries and developed a blockchain scam, known as Marine Chain. North Korean cyber agents have increasingly paid attention to the nexus of cyber and maritime domains in their activities.

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Is Does Failure Mindset Transmitted through Generations?: The Dual Mediating Effects of Mother's Happiness and Mother-Adolescent Open Communication (실패 마인드셋은 세대 간 전승되는가?: 어머니의 행복감과 개방적 의사소통의 이중매개효과)

  • Jung, Jiyu;Kim, Hansaem;Yang, Sujin
    • Korean Journal of Culture and Social Issue
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    • v.28 no.3
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    • pp.419-439
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    • 2022
  • The purpose of this study was to examine the relationship between failure mindset of the mother and adolescent, along with the mediating effects of mother's happiness and mother-adolescents (first and second-year middle school students in Seoul) open communication. For this study, self-reported data of 270 mother-adolescent dyads were analyzed using SPSS and Process macro Model 6. The results revealed that the mother's failure mindset directly predicted their adolescent's failure mindset. The mediating effect of the mother-adolescent open communication was significant, but the mediating effect of the mother's happiness was not significant. However, the mediation effect of the mother's failure mindset on the adolescent's failure mindset through mother's happiness and mother-adolescent open communication was significant. These results suggest that "failure-is-enhancing" mindset of mothers improve their positive affect and parenting skills, which contributes to developing adolescent's belief system about failure. Putting the results together, this study is significant in that it identified the intergenerational relationship of the failure mindset. Besides, the study results serve as a foundation for intervening on the adolescent's failure mindset by verifying the developmental mechanism of the failure mindset.

The Double Mediating Effects of Ego-Resilience and Impulsivity between Smartphone Addiction and Depression among Office Workers (직장인의 스마트폰 중독과 우울감 사이에서 자아탄력성과 충동성의 이중 매개효과)

  • Kwon, Se-yong;Kim, Hyeong-ho
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.6
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    • pp.355-363
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    • 2022
  • Purpose : The purpose of this study was to investigate the double mediating effect of ego-resilience and impulsivity in the effects of smartphone addiction on depression among office workers. Research design, data and methodology : The subjects of this study were office workers working in four cities: Seoul, Daejeon, Chungnam, and Chungbuk Province, and the total number of respondents was 286. Descriptive statistics, difference test (t-test, ANOVA), correlation analysis, and double-mediated effect analysis were used for statistics. Results : The results of this study are as follows. First, as a result of the difference analysis, there was no difference in depression according to the general characteristics of the subjects of this study, such as gender, age, marital status, and salary. Second, as a result of correlation analysis, smartphone addiction showed a negative correlation with ego-resilience, and a positive correlation with impulsivity and depression. Also, ego-resilience showed negative correlation with impulsivity and depression. Impulsivity showed a positive correlation with depression. Third, as a result of dual mediating effect analysis, it was found that ego-resilience and impulsivity played double-mediated role between smartphone addiction and depression. Conclusions : Based on the results of this study, theoretical measures centered on ego-resilience were discussed and presented in order to positively induce the decrease in impulsivity and depression due to smartphone addiction among office workers.

Experience of Korean-Chinese Part-time Job Students in Korea - Approach to the Phenomenological Research Method of Colaizzi- (조선족 아르바이트 유학생의 차별경험 - Colaizzi의 현상학적 연구방법 접근 -)

  • Lin Zheng;WonGyu Choi
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.1
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    • pp.267-279
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    • 2023
  • The purpose of this study is to find out the experience and meaning of discrimination among Korean-Chinese part-time job international students. To this end, after in-depth interviews with nine Korean-Chinese part-time job international students from May to June 2022, the data were analyzed using Colaizzi's phenomenological research method. The findings were as follows: New challenges for living expenses and efficient adaptation, this road full of thorns, efforts to adapt beyond discrimination, growth and relationships through part-time jobs turned out to be opportunities. This experience became a process for Korean-Chinese part-time international students to grow further as well as solve economic problems. However, they are subject to multiple discrimination due to their dual status as international students and ethnic Korean-Chinese. Their Korean identity gives a more directed differential experience in the process of working part-time. But in the process, they are still overcoming difficulties. Korean-Chinese international students are growing up by accepting discrimination experiences and adapting to their international life. As a result of the above research, we need education and help to guarantee working human rights for Korean-Chinese part-time students with the development of a multicultural society in the future.

Analysis on Policy Discourse of Female Traditional Musician in Joseon Era (문화정책 관점에서의 조선시대 여악에 대한 담론 연구)

  • Kwon, Youngji;Hong, Kiwon
    • 지역과문화
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    • v.6 no.3
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    • pp.29-53
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    • 2019
  • Korean traditional women artists are placed in dual suffering from unequal rights in terms of gender and misrecognition endowed by historical legacy. There has been no clear cut definition but interchangeable adoption of various terms such as Yeo-ak, Yeo-gi, and Gisaeng even in the study of music theory and history itself. Study on female musician has been mostly performed on the basis of music theory and history so that one sided discourse on female traditional artist has survived and aggravated its connotation during the colonial ages and modernization. Envisioning traditional female artist as instrumentalizing their body and status as artist resulted in crucifying victims of sexual harassment is one recent example. This study is an attempt to collect knowledge on the various layers of discourse about the status and role of female traditional artist. This is a first stage of analysis covering the period Joseon dynasty where original and official records regarding female traditional artists remains until today. The findings are that policy discourse are to be classified as politico-ideological layer, music theoretical layer, and socio-political layer. It is to be clarified in the future which layer has the most sustaining influence to the present and why.

Exploration of the Current Status of the Career Counseling Teacher Training Course and Improvement Plans (진로 진학 상담 교사 양성과정 현황과 개선 방안 탐색)

  • Yoon Ok Han
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.6
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    • pp.151-160
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    • 2023
  • The purpose of this study is to suggest problems and directions for improvement, focusing on the master's course at the Graduate School of Education, among the career guidance counselor training programs. First, in the case of career education counseling major education graduate school fostering system, ① 3 universities in Seoul, 2 in Gyeonggi-do, 1 in Incheon, 1 in Gangwon, 1 in Daegu, 1 in Jeonbuk, 3 in Chungnam, and 1 in Chungbuk, a total of 13 Opened in 20 universities. ② Most schools operate in a dual system. ③ As a major in career counseling, there is a maximum of 26 courses and a minimum of 10 courses for the master's course in the graduate school of education. Second, what are the problems and improvement directions of the career counseling graduate school training system? ① It is necessary to prepare a standardized curriculum. ② It is necessary to operate education to strengthen connection with the local community. ③ There is a need to resolve the level gap between the 13 graduate schools of education and the lack of faculty members at some universities. ④ Career education policy department of the Ministry of Education needs to periodically evaluate 13 education graduate schools. ⑤ The role of the career policy department of the Ministry of Education is to establish a mid- too long-term plan at the national level considering the demand and supply of career guidance counselors nationwide. ⑥ It is desirable to dualize the way to obtain a career guidance counselor license as it is now.